Recombinant clostridia that fix CO2 and CO and uses thereof
Abstract
The present invention relates a recombinant Clostridium expressing one or more heterologous Wood-Ljungdahl (WL) genes. In particular, the recombinant Clostridium produces a metabolite at an increased level. The present invention also relates to a method for producing a metabolite by the recombinant Clostridium.
- Inventors:
- Issue Date:
- Research Org.:
- University of Delaware, Newark, DE (USA).
- Sponsoring Org.:
- USDOE
- OSTI Identifier:
- 1135908
- Patent Number(s):
- 8759070
- Application Number:
- 13/229,033
- Assignee:
- University of Delaware (Newark, DE)
- Patent Classifications (CPCs):
-
C - CHEMISTRY C07 - ORGANIC CHEMISTRY C07K - PEPTIDES
C - CHEMISTRY C12 - BIOCHEMISTRY C12N - MICROORGANISMS OR ENZYMES
- Resource Type:
- Patent
- Country of Publication:
- United States
- Language:
- English
- Subject:
- 59 BASIC BIOLOGICAL SCIENCES
Citation Formats
Papoutsakis, Eleftherios T., Al-Hinai, Mohab Ali, Jones, Shawn Williams, Indurthi, Dinesh Chanukya, Mitchell, Daniel Knox, and Fast, Alan. Recombinant clostridia that fix CO2 and CO and uses thereof. United States: N. p., 2014.
Web.
Papoutsakis, Eleftherios T., Al-Hinai, Mohab Ali, Jones, Shawn Williams, Indurthi, Dinesh Chanukya, Mitchell, Daniel Knox, & Fast, Alan. Recombinant clostridia that fix CO2 and CO and uses thereof. United States.
Papoutsakis, Eleftherios T., Al-Hinai, Mohab Ali, Jones, Shawn Williams, Indurthi, Dinesh Chanukya, Mitchell, Daniel Knox, and Fast, Alan. Tue .
"Recombinant clostridia that fix CO2 and CO and uses thereof". United States. https://www.osti.gov/servlets/purl/1135908.
@article{osti_1135908,
title = {Recombinant clostridia that fix CO2 and CO and uses thereof},
author = {Papoutsakis, Eleftherios T. and Al-Hinai, Mohab Ali and Jones, Shawn Williams and Indurthi, Dinesh Chanukya and Mitchell, Daniel Knox and Fast, Alan},
abstractNote = {The present invention relates a recombinant Clostridium expressing one or more heterologous Wood-Ljungdahl (WL) genes. In particular, the recombinant Clostridium produces a metabolite at an increased level. The present invention also relates to a method for producing a metabolite by the recombinant Clostridium.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {2014},
month = {6}
}
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Works referenced in this record:
Biological production of acetic acid from waste gases with Clostridium ljungdahlii
patent, September 1998
- Gaddy, James L.
- US Patent Document 5,807,722
Biological production of products from waste gases
patent, January 2002
- Gaddy, James L.
- US Patent Document 6,340,581
Methods and Organisms for Production of 3-Hydroxypropionic Acid
patent-application, January 2010
- Burk, Mark J.; Osterhout, Robin E.
- US Patent Application 12/508547; 20100021978
Methods and Composition for Generating Sporulation Deficient Bacteria
patent-application, February 2010
- Tracy, Bryan P.; Paredes, Carlos J.; Papoutsakis, Eleftherios T.
- US Patent Application 12/485636; 20100047890
Process for the Biological Production of n-Butanol with High Yield
patent-application, April 2010
- Soucaille, Philippe
- US Patent Application 12/447726; 20100086982
Process for the Biological Production of 1.3-Propanediol from Glycerol with High Yield
patent-application, June 2010
- Sourcaille, Philippe
- US Patent Application 12/447686; 20100137655
Transformant Capable of Producing Isopropanol
patent-application, August 2010
- Yukawa, Hideaki; Inui, Masayuki
- US Patent Application 12/733366; 20100203604
Expression of plasmid-encoded aad in Clostridium acetobutylicum M5 restores vigorous butanol production.
journal, September 1994
- Nair, R. V.; Papoutsakis, E. T.
- Journal of Bacteriology, Vol. 176, Issue 18
Basic local alignment search tool
journal, October 1990
- Altschul, Stephen F.; Gish, Warren; Miller, Webb
- Journal of Molecular Biology, Vol. 215, Issue 3, p. 403-410
Systems-Level Metabolic Flux Profiling Elucidates a Complete, Bifurcated Tricarboxylic Acid Cycle in Clostridium acetobutylicum
journal, July 2010
- Amador-Noguez, D.; Feng, X. -J.; Fan, J.
- Journal of Bacteriology, Vol. 192, Issue 17
Clostridium perfringens-Escherichia coli Shuttle Vectors That Carry Single Antibiotic Resistance Determinants
journal, May 1993
- Bannam, Trudi L.; Rood, Julian I.
- Plasmid, Vol. 29, Issue 3
Distribution of Methanol Carbon Between Assimilation and Oxidation Pathways in Methanol-grown Pseudomonas C
journal, January 1980
- Ben-Bassat, A.; Goldberg, I.; Mateles, R. I.
- Microbiology, Vol. 116, Issue 1
Evidence That Ferredoxin Interfaces with an Internal Redox Shuttle in Acetyl-CoA Synthase during Reductive Activation and Catalysis
journal, January 2011
- Bender, Güneş; Ragsdale, Stephen W.
- Biochemistry, Vol. 50, Issue 2
Natural transfer of conjugative transposon Tn916 between gram-positive and gram-negative bacteria.
journal, January 1991
- Bertram, J.; Strätz, M.; Dürre, P.
- Journal of Bacteriology, Vol. 173, Issue 2
A genomic-library based discovery of a novel, possibly synthetic, acid-tolerance mechanism in Clostridium acetobutylicum involving non-coding RNAs and ribosomal RNA processing
journal, May 2010
- Borden, Jacob R.; Jones, Shawn W.; Indurthi, Dinesh
- Metabolic Engineering, Vol. 12, Issue 3, p. 268-281
The genes for butanol and acetone formation in Clostridium acetobutylicum ATCC 824 reside on a large plasmid whose loss leads to degeneration of the strain.
journal, September 1997
- Cornillot, E.; Nair, R. V.; Papoutsakis, E. T.
- Journal of Bacteriology, Vol. 179, Issue 17
Physiology of the thermophilic acetogen Moorella thermoacetica
journal, December 2004
- Drake, Harold L.; Daniel, Steven L.
- Research in Microbiology, Vol. 155, Issue 10, p. 869-883
Bioproduction of butanol from biomass: from genes to bioreactors
journal, June 2007
- Ezeji, Thaddeus Chukwuemeka; Qureshi, Nasib; Blaschek, Hans Peter
- Current Opinion in Biotechnology, Vol. 18, Issue 3, p. 220-227
Effect of nitrate on the autotrophic metabolism of the acetogens Clostridium thermoautotrophicum and Clostridium thermoaceticum.
journal, August 1996
- Fröstl, J. M.; Seifritz, C.; Drake, H. L.
- Journal of Bacteriology, Vol. 178, Issue 15
Identification and molecular genetic analysis of replication functions of the bacteriocinogenic plasmid pIP404 from Clostridium perfringens
journal, March 1988
- Garnier, Thierry; Cole, Stewart T.
- Plasmid, Vol. 19, Issue 2, p. 151-160
Metabolic pathways of clostridia for producing butanol
journal, November 2009
- Gheshlaghi, R.; Scharer, J. M.; Moo-Young, M.
- Biotechnology Advances, Vol. 27, Issue 6, p. 764-781
Fermentation characterization and flux analysis of recombinant strains of Clostridium acetobutylicum with an inactivated solR gene
journal, November 2001
- Harris, Lm; Blank, L.; Desai, Rp
- Journal of Industrial Microbiology and Biotechnology, Vol. 27, Issue 5
Characterization of recombinant strains of the Clostridium acetobutylicum butyrate kinase inactivation mutant: Need for new phenomenological models for solventogenesis and butanol inhibition?
journal, January 2000
- Harris, Latonia M.; Desai, Ruchir P.; Welker, Neil E.
- Biotechnology and Bioengineering, Vol. 67, Issue 1, p. 1-11
Construction and Characterization of a Lactose-Inducible Promoter System for Controlled Gene Expression in Clostridium perfringens
journal, November 2010
- Hartman, Andrea H.; Liu, Hualan; Melville, Stephen B.
- Applied and Environmental Microbiology, Vol. 77, Issue 2
A modular system for Clostridium shuttle plasmids
journal, July 2009
- Heap, John T.; Pennington, Oliver J.; Cartman, Stephen T.
- Journal of Microbiological Methods, Vol. 78, Issue 1, p. 79-85
PerR acts as a switch for oxygen tolerance in the strict anaerobe Clostridium acetobutylicum
journal, May 2008
- Hillmann, Falk; Fischer, Ralf-Jörg; Saint-Prix, Florence
- Molecular Microbiology, Vol. 68, Issue 4, p. 848-860
Purification and Characterization of Membrane-associated CooC Protein and Its Functional Role in the Insertion of Nickel into Carbon Monoxide Dehydrogenase from Rhodospirillum rubrum
journal, August 2001
- Jeon, Won Bae; Cheng, Jiujun; Ludden, Paul W.
- Journal of Biological Chemistry, Vol. 276, Issue 42
The transcriptional program underlying the physiology of clostridial sporulation
journal, January 2008
- Jones, Shawn W.; Paredes, Carlos J.; Tracy, Bryan
- Genome Biology, Vol. 9, Issue 7
Inactivation of F in Clostridium acetobutylicum ATCC 824 Blocks Sporulation Prior to Asymmetric Division and Abolishes E and G Protein Expression but Does Not Block Solvent Formation
journal, March 2011
- Jones, S. W.; Tracy, B. P.; Gaida, S. M.
- Journal of Bacteriology, Vol. 193, Issue 10
KEGG: Kyoto Encyclopedia of Genes and Genomes
journal, January 2000
- Kanehisa, Minoru; Goto, Susumu
- Nucleic Acids Research, Vol. 28, Issue 1, p. 27-30
Clostridium ljungdahlii represents a microbial production platform based on syngas
journal, July 2010
- Kopke, M.; Held, C.; Hujer, S.
- Proceedings of the National Academy of Sciences, Vol. 107, Issue 29, p. 13087-13092
Gas chromatography and gateway sensors for on-line state estimation of complex fermentations (butanol-acetone fermentation)
journal, August 1985
- McLaughlin, Joseph K.; Meyer, Charles L.; Papoutsakis, Eleftherios T.
- Biotechnology and Bioengineering, Vol. 27, Issue 8, p. 1246-1257
Expression of Cloned Homologous Fermentative Genes in Clostridium Acetobutylicum ATCC 824
journal, February 1992
- Mermelstein, Lee D.; Welker, Neil E.; Bennett, George N.
- Nature Biotechnology, Vol. 10, Issue 2
Sequence and properties of pIM13, a macrolide-lincosamide-streptogramin B resistance plasmid from Bacillus subtilis.
journal, July 1986
- Monod, M.; Denoya, C.; Dubnau, D.
- Journal of Bacteriology, Vol. 167, Issue 1
Genome Sequence and Comparative Analysis of the Solvent-Producing Bacterium Clostridium acetobutylicum
journal, August 2001
- Nolling, J.; Breton, G.; Omelchenko, M. V.
- Journal of Bacteriology, Vol. 183, Issue 16
Engineering solventogenic clostridia
journal, October 2008
- Papoutsakis, Eleftherios T.
- Current Opinion in Biotechnology, Vol. 19, Issue 5, p. 420-429
The complete genome sequence of Moorella thermoacetica (f. Clostridium thermoaceticum)
journal, July 2008
- Pierce, Elizabeth; Xie, Gary; Barabote, Ravi D.
- Environmental Microbiology, Vol. 10, Issue 10, p. 2550-2573
Replication properties of pIM13, a naturally occurring plasmid found in Bacillus subtilis, and of its close relative pE5, a plasmid native to Staphylococcus aureus.
journal, November 1987
- Projan, S. J.; Monod, M.; Narayanan, C. S.
- Journal of Bacteriology, Vol. 169, Issue 11
The Eastern and Western branches of the Wood/Ljungdahl pathway: how the East and West were won
journal, January 1997
- Ragsdale, Stephen W.
- BioFactors, Vol. 6, Issue 1
Acetogenesis and the Wood–Ljungdahl pathway of CO2 fixation
journal, December 2008
- Ragsdale, Stephen W.; Pierce, Elizabeth
- Biochimica et Biophysica Acta (BBA) - Proteins and Proteomics, Vol. 1784, Issue 12, p. 1873-1898
Ethanol production from H 2 and CO 2 by a newly isolated thermophilic bacterium, Moorella sp. HUC22-1
journal, October 2004
- Sakai, S.; Nakashimada, Y.; Yoshimoto, H.
- Biotechnology Letters, Vol. 26, Issue 20
Acetate and ethanol production from H2 and CO2by Moorella sp. using a repeated batch culture
journal, March 2005
- Sakai, Shinsuke; Nakashimada, Yutaka; Inokuma, Kentaro
- Journal of Bioscience and Bioengineering, Vol. 99, Issue 3
Optimization of a heterologous pathway for the production of flavonoids from glucose
journal, July 2011
- Santos, Christine Nicole S.; Koffas, Mattheos; Stephanopoulos, Gregory
- Metabolic Engineering, Vol. 13, Issue 4, p. 392-400
Isolation of mutants ofClostridium acetobutylicum ATCC 824 deficient in protease activity
journal, March 1993
- Sass, Catherine; Walter, Joe; Bennett, George N.
- Current Microbiology, Vol. 26, Issue 3
The multidrug-resistant human pathogen Clostridium difficile has a highly mobile, mosaic genome
journal, June 2006
- Sebaihia, Mohammed; Wren, Brendan W.; Mullany, Peter
- Nature Genetics, Vol. 38, Issue 7
Nitrate as a preferred electron sink for the acetogen Clostridium thermoaceticum.
journal, December 1993
- Seifritz, C.; Daniel, S. L.; Gössner, A.
- Journal of Bacteriology, Vol. 175, Issue 24
Metabolic engineering of the non-sporulating, non-solventogenic Clostridium acetobutylicum strain M5 to produce butanol without acetone demonstrate the robustness of the acid-formation pathways and the importance of the electron balance
journal, November 2008
- Sillers, Ryan; Chow, Alison; Tracy, Bryan
- Metabolic Engineering, Vol. 10, Issue 6, p. 321-332
Aldehyde-alcohol dehydrogenase and/or thiolase overexpression coupled with CoA transferase downregulation lead to higher alcohol titers and selectivity in Clostridium acetobutylicum fermentations
journal, January 2009
- Sillers, Ryan; Al-Hinai, Mohab Ali; Papoutsakis, Eleftherios T.
- Biotechnology and Bioengineering, Vol. 102, Issue 1, p. 38-49
Analysis of Degenerate Variants ofClostridium acetobutylicumATCC 824
journal, February 1996
- Stim-Herndon, K. P.; Nair, R.; Papoutsakis, E. T.
- Anaerobe, Vol. 2, Issue 1
DNA Array-Based Transcriptional Analysis of Asporogenous, Nonsolventogenic Clostridium acetobutylicum Strains SKO1 and M5
journal, July 2003
- Tomas, C. A.; Alsaker, K. V.; Bonarius, H. P. J.
- Journal of Bacteriology, Vol. 185, Issue 15
Inactivation of E and G in Clostridium acetobutylicum Illuminates Their Roles in Clostridial-Cell-Form Biogenesis, Granulose Synthesis, Solventogenesis, and Spore Morphogenesis
journal, January 2011
- Tracy, B. P.; Jones, S. W.; Papoutsakis, E. T.
- Journal of Bacteriology, Vol. 193, Issue 6
Genetic Methods in Clostridia
book, January 1999
- Young, D. I.; Evans, V. J.; Jefferies, J. R.
- Methods in Microbiology
mazF, a novel counter-selectable marker for unmarked chromosomal manipulation in Bacillus subtilis
journal, January 2006
- Zhang, X. -Z.; Yan, X.; Cui, Z. -L.
- Nucleic Acids Research, Vol. 34, Issue 9
Works referencing / citing this record:
Recombinant clostridia that fix CO2 and CO and uses thereof
patent, June 2014
- Papoutsakis, Eleftherios T.; Al-Hinai, Mohab Ali; Jones, Shawn William
- US Patent Document 8,759,070