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Title: Recombinant clostridia that fix CO2 and CO and uses thereof

The present invention relates a recombinant Clostridium expressing one or more heterologous Wood-Ljungdahl (WL) genes. In particular, the recombinant Clostridium produces a metabolite at an increased level. The present invention also relates to a method for producing a metabolite by the recombinant Clostridium.
Inventors:
; ; ; ; ;
Issue Date:
OSTI Identifier:
1135908
Assignee:
University of Delaware (Newark, DE) ARPA-E
Patent Number(s):
8,759,070
Application Number:
13/229,033
Research Org:
University of Delaware, Newark, DE (USA).
Sponsoring Org:
USDOE
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES

Other works cited in this record:

Basic local alignment search tool
journal, October 1990
  • Altschul, Stephen F.; Gish, Warren; Miller, Webb
  • Journal of Molecular Biology, Vol. 215, Issue 3, p. 403-410
  • DOI: 10.1016/S0022-2836(05)80360-2

A genomic-library based discovery of a novel, possibly synthetic, acid-tolerance mechanism in Clostridium acetobutylicum involving non-coding RNAs and ribosomal RNA processing
journal, May 2010
  • Borden, Jacob R.; Jones, Shawn W.; Indurthi, Dinesh
  • Metabolic Engineering, Vol. 12, Issue 3, p. 268-281
  • DOI: 10.1016/j.ymben.2009.12.004

Physiology of the thermophilic acetogen Moorella thermoacetica
journal, December 2004

Bioproduction of butanol from biomass: from genes to bioreactors
journal, June 2007
  • Ezeji, Thaddeus Chukwuemeka; Qureshi, Nasib; Blaschek, Hans Peter
  • Current Opinion in Biotechnology, Vol. 18, Issue 3, p. 220-227
  • DOI: 10.1016/j.copbio.2007.04.002

Identification and molecular genetic analysis of replication functions of the bacteriocinogenic plasmid pIP404 from Clostridium perfringens
journal, March 1988

Metabolic pathways of clostridia for producing butanol
journal, November 2009

Characterization of recombinant strains of the Clostridium acetobutylicum butyrate kinase inactivation mutant: Need for new phenomenological models for solventogenesis and butanol inhibition?
journal, January 2000

A modular system for Clostridium shuttle plasmids
journal, July 2009
  • Heap, John T.; Pennington, Oliver J.; Cartman, Stephen T.
  • Journal of Microbiological Methods, Vol. 78, Issue 1, p. 79-85
  • DOI: 10.1016/j.mimet.2009.05.004

PerR acts as a switch for oxygen tolerance in the strict anaerobe Clostridium acetobutylicum
journal, May 2008

KEGG: Kyoto Encyclopedia of Genes and Genomes
journal, January 2000
  • Kanehisa, Minoru; Goto, Susumu
  • Nucleic Acids Research, Vol. 28, Issue 1, p. 27-30
  • DOI: 10.1093/nar/28.1.27

Clostridium ljungdahlii represents a microbial production platform based on syngas
journal, July 2010
  • Kopke, M.; Held, C.; Hujer, S.
  • Proceedings of the National Academy of Sciences, Vol. 107, Issue 29, p. 13087-13092
  • DOI: 10.1073/pnas.1004716107

Gas chromatography and gateway sensors for on-line state estimation of complex fermentations (butanol-acetone fermentation)
journal, August 1985
  • McLaughlin, Joseph K.; Meyer, Charles L.; Papoutsakis, Eleftherios T.
  • Biotechnology and Bioengineering, Vol. 27, Issue 8, p. 1246-1257
  • DOI: 10.1002/bit.260270821

Engineering solventogenic clostridia
journal, October 2008

Acetogenesis and the Wood–Ljungdahl pathway of CO2 fixation
journal, December 2008
  • Ragsdale, Stephen W.; Pierce, Elizabeth
  • Biochimica et Biophysica Acta (BBA) - Proteins and Proteomics, Vol. 1784, Issue 12, p. 1873-1898
  • DOI: 10.1016/j.bbapap.2008.08.012

Optimization of a heterologous pathway for the production of flavonoids from glucose
journal, July 2011
  • Santos, Christine Nicole S.; Koffas, Mattheos; Stephanopoulos, Gregory
  • Metabolic Engineering, Vol. 13, Issue 4, p. 392-400
  • DOI: 10.1016/j.ymben.2011.02.002

Metabolic engineering of the non-sporulating, non-solventogenic Clostridium acetobutylicum strain M5 to produce butanol without acetone demonstrate the robustness of the acid-formation pathways and the importance of the electron balance
journal, November 2008

Aldehyde-alcohol dehydrogenase and/or thiolase overexpression coupled with CoA transferase downregulation lead to higher alcohol titers and selectivity in Clostridium acetobutylicum fermentations
journal, January 2009
  • Sillers, Ryan; Al-Hinai, Mohab Ali; Papoutsakis, Eleftherios T.
  • Biotechnology and Bioengineering, Vol. 102, Issue 1, p. 38-49
  • DOI: 10.1002/bit.22058

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