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Title: Recombinant clostridia that fix CO2 and CO and uses thereof

Abstract

The present invention relates a recombinant Clostridium expressing one or more heterologous Wood-Ljungdahl (WL) genes. In particular, the recombinant Clostridium produces a metabolite at an increased level. The present invention also relates to a method for producing a metabolite by the recombinant Clostridium.

Inventors:
; ; ; ; ;
Issue Date:
Research Org.:
University of Delaware, Newark, DE (USA).
Sponsoring Org.:
USDOE
OSTI Identifier:
1135908
Patent Number(s):
8,759,070
Application Number:
13/229,033
Assignee:
University of Delaware (Newark, DE)
Resource Type:
Patent
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES

Citation Formats

Papoutsakis, Eleftherios T., Al-Hinai, Mohab Ali, Jones, Shawn Williams, Indurthi, Dinesh Chanukya, Mitchell, Daniel Knox, and Fast, Alan. Recombinant clostridia that fix CO2 and CO and uses thereof. United States: N. p., 2014. Web.
Papoutsakis, Eleftherios T., Al-Hinai, Mohab Ali, Jones, Shawn Williams, Indurthi, Dinesh Chanukya, Mitchell, Daniel Knox, & Fast, Alan. Recombinant clostridia that fix CO2 and CO and uses thereof. United States.
Papoutsakis, Eleftherios T., Al-Hinai, Mohab Ali, Jones, Shawn Williams, Indurthi, Dinesh Chanukya, Mitchell, Daniel Knox, and Fast, Alan. Tue . "Recombinant clostridia that fix CO2 and CO and uses thereof". United States. https://www.osti.gov/servlets/purl/1135908.
@article{osti_1135908,
title = {Recombinant clostridia that fix CO2 and CO and uses thereof},
author = {Papoutsakis, Eleftherios T. and Al-Hinai, Mohab Ali and Jones, Shawn Williams and Indurthi, Dinesh Chanukya and Mitchell, Daniel Knox and Fast, Alan},
abstractNote = {The present invention relates a recombinant Clostridium expressing one or more heterologous Wood-Ljungdahl (WL) genes. In particular, the recombinant Clostridium produces a metabolite at an increased level. The present invention also relates to a method for producing a metabolite by the recombinant Clostridium.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {2014},
month = {6}
}

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Works referenced in this record:

Expression of plasmid-encoded aad in Clostridium acetobutylicum M5 restores vigorous butanol production.
journal, September 1994


Basic local alignment search tool
journal, October 1990

  • Altschul, Stephen F.; Gish, Warren; Miller, Webb
  • Journal of Molecular Biology, Vol. 215, Issue 3, p. 403-410
  • DOI: 10.1016/S0022-2836(05)80360-2

Systems-Level Metabolic Flux Profiling Elucidates a Complete, Bifurcated Tricarboxylic Acid Cycle in Clostridium acetobutylicum
journal, July 2010

  • Amador-Noguez, D.; Feng, X. -J.; Fan, J.
  • Journal of Bacteriology, Vol. 192, Issue 17
  • DOI: 10.1128/JB.00490-10

Distribution of Methanol Carbon Between Assimilation and Oxidation Pathways in Methanol-grown Pseudomonas C
journal, January 1980


Evidence That Ferredoxin Interfaces with an Internal Redox Shuttle in Acetyl-CoA Synthase during Reductive Activation and Catalysis
journal, January 2011

  • Bender, Güneş; Ragsdale, Stephen W.
  • Biochemistry, Vol. 50, Issue 2
  • DOI: 10.1021/bi101511r

Natural transfer of conjugative transposon Tn916 between gram-positive and gram-negative bacteria.
journal, January 1991


Physiology of the thermophilic acetogen Moorella thermoacetica
journal, December 2004


Bioproduction of butanol from biomass: from genes to bioreactors
journal, June 2007

  • Ezeji, Thaddeus Chukwuemeka; Qureshi, Nasib; Blaschek, Hans Peter
  • Current Opinion in Biotechnology, Vol. 18, Issue 3, p. 220-227
  • DOI: 10.1016/j.copbio.2007.04.002

Effect of nitrate on the autotrophic metabolism of the acetogens Clostridium thermoautotrophicum and Clostridium thermoaceticum.
journal, August 1996


Metabolic pathways of clostridia for producing butanol
journal, November 2009


Fermentation characterization and flux analysis of recombinant strains of Clostridium acetobutylicum with an inactivated solR gene
journal, November 2001

  • Harris, Lm; Blank, L.; Desai, Rp
  • Journal of Industrial Microbiology and Biotechnology, Vol. 27, Issue 5
  • DOI: 10.1038/sj.jim.7000191

Construction and Characterization of a Lactose-Inducible Promoter System for Controlled Gene Expression in Clostridium perfringens
journal, November 2010

  • Hartman, Andrea H.; Liu, Hualan; Melville, Stephen B.
  • Applied and Environmental Microbiology, Vol. 77, Issue 2
  • DOI: 10.1128/AEM.01536-10

A modular system for Clostridium shuttle plasmids
journal, July 2009

  • Heap, John T.; Pennington, Oliver J.; Cartman, Stephen T.
  • Journal of Microbiological Methods, Vol. 78, Issue 1, p. 79-85
  • DOI: 10.1016/j.mimet.2009.05.004

PerR acts as a switch for oxygen tolerance in the strict anaerobe Clostridium acetobutylicum
journal, May 2008


The transcriptional program underlying the physiology of clostridial sporulation
journal, January 2008


KEGG: Kyoto Encyclopedia of Genes and Genomes
journal, January 2000

  • Kanehisa, Minoru; Goto, Susumu
  • Nucleic Acids Research, Vol. 28, Issue 1, p. 27-30
  • DOI: 10.1093/nar/28.1.27

Clostridium ljungdahlii represents a microbial production platform based on syngas
journal, July 2010

  • Kopke, M.; Held, C.; Hujer, S.
  • Proceedings of the National Academy of Sciences, Vol. 107, Issue 29, p. 13087-13092
  • DOI: 10.1073/pnas.1004716107

Gas chromatography and gateway sensors for on-line state estimation of complex fermentations (butanol-acetone fermentation)
journal, August 1985

  • McLaughlin, Joseph K.; Meyer, Charles L.; Papoutsakis, Eleftherios T.
  • Biotechnology and Bioengineering, Vol. 27, Issue 8, p. 1246-1257
  • DOI: 10.1002/bit.260270821

Expression of Cloned Homologous Fermentative Genes in Clostridium Acetobutylicum ATCC 824
journal, February 1992

  • Mermelstein, Lee D.; Welker, Neil E.; Bennett, George N.
  • Nature Biotechnology, Vol. 10, Issue 2
  • DOI: 10.1038/nbt0292-190

Sequence and properties of pIM13, a macrolide-lincosamide-streptogramin B resistance plasmid from Bacillus subtilis.
journal, July 1986


Genome Sequence and Comparative Analysis of the Solvent-Producing Bacterium Clostridium acetobutylicum
journal, August 2001


Engineering solventogenic clostridia
journal, October 2008


Genome Sequence of the Solvent-Producing Bacterium Clostridium carboxidivorans Strain P7T
journal, August 2010

  • Paul, D.; Austin, F. W.; Arick, T.
  • Journal of Bacteriology, Vol. 192, Issue 20, p. 5554-5555
  • DOI: 10.1128/JB.00877-10

The complete genome sequence of Moorella thermoacetica (f. Clostridium thermoaceticum)
journal, July 2008


The Eastern and Western branches of the Wood/Ljungdahl pathway: how the East and West were won
journal, January 1997


Acetogenesis and the Wood–Ljungdahl pathway of CO2 fixation
journal, December 2008

  • Ragsdale, Stephen W.; Pierce, Elizabeth
  • Biochimica et Biophysica Acta (BBA) - Proteins and Proteomics, Vol. 1784, Issue 12, p. 1873-1898
  • DOI: 10.1016/j.bbapap.2008.08.012

Ethanol production from H 2 and CO 2 by a newly isolated thermophilic bacterium, Moorella sp. HUC22-1
journal, October 2004


Acetate and ethanol production from H2 and CO2by Moorella sp. using a repeated batch culture
journal, March 2005

  • Sakai, Shinsuke; Nakashimada, Yutaka; Inokuma, Kentaro
  • Journal of Bioscience and Bioengineering, Vol. 99, Issue 3
  • DOI: 10.1263/jbb.99.252

Optimization of a heterologous pathway for the production of flavonoids from glucose
journal, July 2011

  • Santos, Christine Nicole S.; Koffas, Mattheos; Stephanopoulos, Gregory
  • Metabolic Engineering, Vol. 13, Issue 4, p. 392-400
  • DOI: 10.1016/j.ymben.2011.02.002

Isolation of mutants ofClostridium acetobutylicum ATCC 824 deficient in protease activity
journal, March 1993

  • Sass, Catherine; Walter, Joe; Bennett, George N.
  • Current Microbiology, Vol. 26, Issue 3
  • DOI: 10.1007/BF01577370

The multidrug-resistant human pathogen Clostridium difficile has a highly mobile, mosaic genome
journal, June 2006

  • Sebaihia, Mohammed; Wren, Brendan W.; Mullany, Peter
  • Nature Genetics, Vol. 38, Issue 7
  • DOI: 10.1038/ng1830

Nitrate as a preferred electron sink for the acetogen Clostridium thermoaceticum.
journal, December 1993


Aldehyde-alcohol dehydrogenase and/or thiolase overexpression coupled with CoA transferase downregulation lead to higher alcohol titers and selectivity in Clostridium acetobutylicum fermentations
journal, January 2009

  • Sillers, Ryan; Al-Hinai, Mohab Ali; Papoutsakis, Eleftherios T.
  • Biotechnology and Bioengineering, Vol. 102, Issue 1, p. 38-49
  • DOI: 10.1002/bit.22058

Analysis of Degenerate Variants ofClostridium acetobutylicumATCC 824
journal, February 1996


DNA Array-Based Transcriptional Analysis of Asporogenous, Nonsolventogenic Clostridium acetobutylicum Strains SKO1 and M5
journal, July 2003


mazF, a novel counter-selectable marker for unmarked chromosomal manipulation in Bacillus subtilis
journal, January 2006

  • Zhang, X. -Z.; Yan, X.; Cui, Z. -L.
  • Nucleic Acids Research, Vol. 34, Issue 9
  • DOI: 10.1093/nar/gkl358