High processivity polymerases
Abstract
Chimeric proteins comprising a sequence nonspecific single-stranded nucleic-acid-binding domain joined to a catalytic nucleic-acid-modifying domain are provided. Methods comprising contacting a nucleic acid molecule with a chimeric protein, as well as systems comprising a nucleic acid molecule, a chimeric protein, and an aqueous solution are also provided. The joining of sequence nonspecific single-stranded nucleic-acid-binding domain and a catalytic nucleic-acid-modifying domain in chimeric proteins, among other things, may prevent the separation of the two domains due to their weak association and thereby enhances processivity while maintaining fidelity.
- Inventors:
- Issue Date:
- Research Org.:
- William Marsh Rice University, Houston, TX (USA)
- Sponsoring Org.:
- USDOE
- OSTI Identifier:
- 1134259
- Patent Number(s):
- 8748147
- Application Number:
- 11/945,877
- Assignee:
- William Marsh Rice University (Houston, TX)
- Patent Classifications (CPCs):
-
C - CHEMISTRY C12 - BIOCHEMISTRY C12Q - MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS
C - CHEMISTRY C07 - ORGANIC CHEMISTRY C07K - PEPTIDES
- DOE Contract Number:
- W-31-109-ENG-38
- Resource Type:
- Patent
- Resource Relation:
- Patent File Date: 2007 Nov 27
- Country of Publication:
- United States
- Language:
- English
- Subject:
- 59 BASIC BIOLOGICAL SCIENCES
Citation Formats
Shamoo, Yousif, and Sun, Siyang. High processivity polymerases. United States: N. p., 2014.
Web.
Shamoo, Yousif, & Sun, Siyang. High processivity polymerases. United States.
Shamoo, Yousif, and Sun, Siyang. Tue .
"High processivity polymerases". United States. https://www.osti.gov/servlets/purl/1134259.
@article{osti_1134259,
title = {High processivity polymerases},
author = {Shamoo, Yousif and Sun, Siyang},
abstractNote = {Chimeric proteins comprising a sequence nonspecific single-stranded nucleic-acid-binding domain joined to a catalytic nucleic-acid-modifying domain are provided. Methods comprising contacting a nucleic acid molecule with a chimeric protein, as well as systems comprising a nucleic acid molecule, a chimeric protein, and an aqueous solution are also provided. The joining of sequence nonspecific single-stranded nucleic-acid-binding domain and a catalytic nucleic-acid-modifying domain in chimeric proteins, among other things, may prevent the separation of the two domains due to their weak association and thereby enhances processivity while maintaining fidelity.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {2014},
month = {6}
}
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