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Title: Compositions and methods for improved protein production

Abstract

The present invention relates to the identification of novel nucleic acid sequences, designated herein as 7p, 8k, 7E, 9G, 8Q and 203, in a host cell which effect protein production. The present invention also provides host cells having a mutation or deletion of part or all of the gene encoding 7p, 8k, 7E, 9G, 8Q and 203, which are presented in FIG. 1, and are SEQ ID NOS.: 1-6, respectively. The present invention also provides host cells further comprising a nucleic acid encoding a desired heterologous protein such as an enzyme.

Inventors:
;
Issue Date:
Research Org.:
National Renewable Energy Lab. (NREL), Golden, CO (United States)
Sponsoring Org.:
USDOE
OSTI Identifier:
1133651
Patent Number(s):
8,741,654
Application Number:
13/494,889
Assignee:
Danisco US Inc. (Palo Alto, CA)
DOE Contract Number:  
AC36-99GO10337
Resource Type:
Patent
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES

Citation Formats

Bodie, Elizabeth A., and Kim, Steve Sungjin. Compositions and methods for improved protein production. United States: N. p., 2014. Web.
Bodie, Elizabeth A., & Kim, Steve Sungjin. Compositions and methods for improved protein production. United States.
Bodie, Elizabeth A., and Kim, Steve Sungjin. Tue . "Compositions and methods for improved protein production". United States. https://www.osti.gov/servlets/purl/1133651.
@article{osti_1133651,
title = {Compositions and methods for improved protein production},
author = {Bodie, Elizabeth A. and Kim, Steve Sungjin},
abstractNote = {The present invention relates to the identification of novel nucleic acid sequences, designated herein as 7p, 8k, 7E, 9G, 8Q and 203, in a host cell which effect protein production. The present invention also provides host cells having a mutation or deletion of part or all of the gene encoding 7p, 8k, 7E, 9G, 8Q and 203, which are presented in FIG. 1, and are SEQ ID NOS.: 1-6, respectively. The present invention also provides host cells further comprising a nucleic acid encoding a desired heterologous protein such as an enzyme.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {2014},
month = {6}
}

Patent:

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Works referenced in this record:

Basic local alignment search tool
journal, October 1990

  • Altschul, Stephen F.; Gish, Warren; Miller, Webb
  • Journal of Molecular Biology, Vol. 215, Issue 3, p. 403-410
  • DOI: 10.1016/S0022-2836(05)80360-2

Strategies and applications of in vitro mutagenesis
journal, September 1985


Genetic transformation of intact cells of Bacillus subtilis by electroporation
journal, January 1990


Gene transfer to plants by diverse species of bacteria
journal, February 2005

  • Broothaerts, Wim; Mitchell, Heidi J.; Weir, Brian
  • Nature, Vol. 433, Issue 7026
  • DOI: 10.1038/nature03309

Gene exchange by design
journal, February 2005


Transformation of Trichoderma harzianum by high-voltage electric pulse
journal, February 1990

  • Goldman, G. H.; Van Montagu, M.; Herrera-Estrella, A.
  • Current Genetics, Vol. 17, Issue 2, p. 169-174
  • DOI: 10.1007/BF00312863

Transformation of Aspergillus awamori by Agrobacterium tumefaciens–mediated homologous recombination
journal, June 1999

  • Gouka, Robin J.; Gerk, Casper; Hooykaas, Paul J. J.
  • Nature Biotechnology, Vol. 17, Issue 6
  • DOI: 10.1038/9915

The small, versatilepPZP family ofAgrobacterium binary vectors for plant transformation
journal, September 1994

  • Hajdukiewicz, Peter; Svab, Zora; Maliga, Pal
  • Plant Molecular Biology, Vol. 25, Issue 6, p. 989-994
  • DOI: 10.1007/BF00014672

A general method of in vitro preparation and specific mutagenesis of DNA fragments: study of protein and DNA interactions
journal, January 1988

  • Higuchi, Russell; Krummel, Barbara; Saiki, Randall
  • Nucleic Acids Research, Vol. 16, Issue 15
  • DOI: 10.1093/nar/16.15.7351

Site-directed mutagenesis by overlap extension using the polymerase chain reaction
journal, April 1989


NewAgrobacterium helper plasmids for gene transfer to plants
journal, July 1993

  • Hood, Elizabeth E.; Gelvin, Stanton B.; Melchers, Leo S.
  • Transgenic Research, Vol. 2, Issue 4
  • DOI: 10.1007/BF01977351

Engineering hybrid genes without the use of restriction enzymes: gene splicing by overlap extension
journal, April 1989


Plasmid transformation in Bacillus subtilis: Symmetry of gene conversion in transformation with a hybrid plasmid containing chromosomal DNA
journal, February 1983

  • Iglesias, A.; Trautner, T. A.
  • MGG Molecular & General Genetics, Vol. 189, Issue 1
  • DOI: 10.1007/BF00326057

Efficient isolation and mapping of Arabidopsis thaliana T-DNA insert junctions by thermal asymmetric interlaced PCR
journal, September 1995


Biolistic transformation of Trichoderma harzianum and Gliocladium virens using plasmid and genomic DNA
journal, October 1993

  • Lorito, M.; Hayes, C. K.; Di Pietro, A.
  • Current Genetics, Vol. 24, Issue 4, p. 349-356
  • DOI: 10.1007/BF00336788

Primary structure of the trpC gene from Aspergillus nidulans
journal, January 1985

  • Mullaney, Edward J.; Hamer, John E.; Roberti, Kellee A.
  • MGG Molecular & General Genetics, Vol. 199, Issue 1, p. 37-45
  • DOI: 10.1007/BF00327506

Agrobacterium -Mediated Transformation of Fusarium oxysporum : An Efficient Tool for Insertional Mutagenesis and Gene Transfer
journal, February 2001


A general method applicable to the search for similarities in the amino acid sequence of two proteins
journal, March 1970


Molecular cloning and characterization of the glucoamylase gene of Aspergillus awamori.
journal, November 1984

  • Nunberg, J. H.; Meade, J. H.; Cole, G.
  • Molecular and Cellular Biology, Vol. 4, Issue 11, p. 2306-2315
  • DOI: 10.1128/MCB.4.11.2306

A versatile transformation system for the cellulolytic filamentous fungus Trichoderma reesei
journal, January 1987


Cellulases of Trichoderma reesei
journal, January 1988


Sequence of the cloned pyr4 gene of Trichoderma reesei and its use as a homologous selectable marker for transformation
journal, January 1991

  • Smith, Jeffrey L.; Bayliss, Frank T.; Ward, Michael
  • Current Genetics, Vol. 19, Issue 1
  • DOI: 10.1007/BF00362084

Comparison of biosequences
journal, December 1981


Successive Construction of Cellulase Hyperproducers of Trichoderma Using Hyperpolyploids
journal, January 2000


Production of recombinant proteins in the filamentous fungus Trichoderma reesei
journal, January 1995


Enhanced production of cellobiohydrolases in Trichoderma reesei and evaluation of the new preparations in biofinishing of cotton
journal, March 2005

  • Miettinen-Oinonen, Arja; Paloheimo, Marja; Lantto, Raija
  • Journal of Biotechnology, Vol. 116, Issue 3, p. 305-317
  • DOI: 10.1016/j.jbiotec.2004.10.017

Filamentous fungi as cell factories for heterologous protein production
journal, May 2002


Construction of cellulase hyper-producers of Trichoderma reesei Rut C-30 for utilization of waste paper using colchicine and benomyl
journal, June 2002

  • Toyama, Hideo; Yamagishi, Naotsugu; Toyama, Nobuo
  • Journal of Molecular Catalysis B: Enzymatic, Vol. 17, Issue 3-5, p. 175-178
  • DOI: 10.1016/S1381-1177(02)00025-5