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Title: Methods for targetted mutagenesis in gram-positive bacteria

The present invention provides a method of targeted mutagenesis in Gram-positive bacteria. In particular, the present invention provides a method that effectively integrates a suicide integrative vector into a target gene in the chromosome of a Gram-positive bacterium, resulting in inactivation of the target gene.
Inventors:
Issue Date:
OSTI Identifier:
1132802
Assignee:
UT-Battelle, LLC (Oak Ridge, TN) ORNL
Patent Number(s):
8,735,160
Application Number:
12/233,806
Contract Number:
AC05-000R22725
Resource Relation:
Patent File Date: 2008 Sep 19
Research Org:
Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States)
Sponsoring Org:
USDOE
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES

Works referenced in this record:

Trans-complementation-dependent replication of a low molecular weight origin fragment from plasmid R6K
journal, December 1978

Microbial Cellulose Utilization: Fundamentals and Biotechnology
journal, September 2002
  • Lynd, L. R.; Weimer, P. J.; van Zyl, W. H.
  • Microbiology and Molecular Biology Reviews, Vol. 66, Issue 3, p. 506-577
  • DOI: 10.1128/MMBR.66.3.506-577.2002