Methods of increasing secretion of polypeptides having biological activity

Merino, Sandra

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Title: Methods of increasing secretion of polypeptides having biological activity

Abstract

The present invention relates to methods for producing a secreted polypeptide having biological activity, comprising: (a) transforming a fungal host cell with a fusion protein construct encoding a fusion protein, which comprises: (i) a first polynucleotide encoding a signal peptide; (ii) a second polynucleotide encoding at least a catalytic domain of an endoglucanase or a portion thereof; and (iii) a third polynucleotide encoding at least a catalytic domain of a polypeptide having biological activity; wherein the signal peptide and at least the catalytic domain of the endoglucanase increases secretion of the polypeptide having biological activity compared to the absence of at least the catalytic domain of the endoglucanase; (b) cultivating the transformed fungal host cell under conditions suitable for production of the fusion protein; and (c) recovering the fusion protein, a component thereof, or a combination thereof, having biological activity, from the cultivation medium.

Inventors:: Merino, Sandra

Issue Date:: 2014-05-27

Research Org.:: National Renewable Energy Laboratory (NREL), Golden, CO (United States)

Sponsoring Org.:: USDOE

OSTI Identifier:: 1132779

Patent Number(s):: 8735549

Application Number:: 14/024,292

Assignee:: Novozymes, Inc. (Davis, CA)

Patent Classifications (CPCs):: C - CHEMISTRY C07 - ORGANIC CHEMISTRY C07K - PEPTIDES

C - CHEMISTRY C12 - BIOCHEMISTRY C12N - MICROORGANISMS OR ENZYMES

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C - CHEMISTRY C07 - ORGANIC CHEMISTRY C07K - PEPTIDES
C07K1/00 - General methods for the preparation of peptides {, i.e. processes for the organic chemical preparation of peptides or proteins of any length}
C07K2319/00 - Fusion polypeptide
C07K2319/036 - targeting to the medium outside of the cell, e.g. type III secretion

C - CHEMISTRY C12 - BIOCHEMISTRY C12N - MICROORGANISMS OR ENZYMES
C12N15/80 - for fungi
C12N9/2434 - {acting on beta-1,4-glucosidic bonds}
C12N9/2437 - {Cellulases
C12N9/2445 - {Beta-glucosidase

C - CHEMISTRY C12 - BIOCHEMISTRY C12P - FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE {
C12P19/02 - Monosaccharides
C12P19/14 - produced by the action of a carbohydrase {
C12P21/02 - having a known sequence of two or more amino acids, e.g. glutathione

C - CHEMISTRY C12 - BIOCHEMISTRY C12Y - ENZYMES
C12Y302/01004 - Cellulase

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DOE Contract Number:: AC36-98GO10337

Resource Type:: Patent

Resource Relation:: Patent File Date: 2013 Sep 11

Country of Publication:: United States

Language:: English

Subject:: 59 BASIC BIOLOGICAL SCIENCES

Citation Formats


                    Merino, Sandra. Methods of increasing secretion of polypeptides having biological activity.  United States: N. p., 2014. 
        Web.

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                    Merino, Sandra. Methods of increasing secretion of polypeptides having biological activity.  United States.

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                    Merino, Sandra. Tue .  
        "Methods of increasing secretion of polypeptides having biological activity".  United States.  https://www.osti.gov/servlets/purl/1132779.

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@article{osti_1132779,

  title        = {Methods of increasing secretion of polypeptides having biological activity},

  author       = {Merino, Sandra},

  abstractNote = {The present invention relates to methods for producing a secreted polypeptide having biological activity, comprising: (a) transforming a fungal host cell with a fusion protein construct encoding a fusion protein, which comprises: (i) a first polynucleotide encoding a signal peptide; (ii) a second polynucleotide encoding at least a catalytic domain of an endoglucanase or a portion thereof; and (iii) a third polynucleotide encoding at least a catalytic domain of a polypeptide having biological activity; wherein the signal peptide and at least the catalytic domain of the endoglucanase increases secretion of the polypeptide having biological activity compared to the absence of at least the catalytic domain of the endoglucanase; (b) cultivating the transformed fungal host cell under conditions suitable for production of the fusion protein; and (c) recovering the fusion protein, a component thereof, or a combination thereof, having biological activity, from the cultivation medium.},

  doi          = {},

  journal      = {},
number       = ,

  volume       = ,

  place        = {United States},

  year         = {2014},

  month        = {5}

}

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Works referenced in this record:

Methods for producing secreted polypeptides
patent, July 2008

Maiyuran, Suchindra; Fidantsef, Ana; Brody, Howard
US Patent Document 7,393,664
URL: https://image-ppubs.uspto.gov/dirsearch-public/print/downloadPdf/7393664

High-throughput screening of expressed DNA libraries in filamentous fungi
patent-application, August 2003

Emalfarb, Mark A.; Punt, Peter J.; Zeijl, Cornelia van
US Patent Application 09/834434; 20030162218
URL: https://image-ppubs.uspto.gov/dirsearch-public/print/downloadPdf/20030162218

Production of functional antibodies in filamentous fungi
patent-application, January 2004

Power, Scott D.; Wang, Huaming; Ward, Michael
US Patent Application 10/418836; 20040018573
URL: https://image-ppubs.uspto.gov/dirsearch-public/print/downloadPdf/20040018573

Methods for degrading lignocellulosic materials
patent-application, July 2005

Vlasenko, Elena; Cherry, Joel; Xu, Feng
US Patent Application 11/036871; 20050164355
URL: https://image-ppubs.uspto.gov/dirsearch-public/print/downloadPdf/20050164355

Methods of producing mutant polynucleotides
patent-application, January 2006

Hansen, Peter Kamp; Bjoernvad, Mads Eskelund; Cherry, Joel
US Patent Application 11/186641; 20060019301
URL: https://image-ppubs.uspto.gov/dirsearch-public/print/downloadPdf/20060019301

Enzyme fusion proteins and their use
patent-application, October 2007

Alapuranen, Marika; Vasltakari, Leena; Kallio, Jarno
US Patent Application 11/404065; 20070244020
URL: https://image-ppubs.uspto.gov/dirsearch-public/print/downloadPdf/20070244020

Strategies for improving heterologous protein production from filamentous fungi
journal, January 1994

Archer, David B.; Jeenes, David J.; Mackenzie, Donald A.
Antonie van Leeuwenhoek, Vol. 65, Issue 3
https://doi.org/10.1007/BF00871952

Cyclic fed-batch culture for production of human serum albumin in Pichia pastoris
journal, June 2003

Bushell, M. E.; Rowe, M.; Avignone-Rossa, C. A.
Biotechnology and Bioengineering, Vol. 82, Issue 6
https://doi.org/10.1002/bit.10616

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Similar Records

Title: Methods of increasing secretion of polypeptides having biological activity

Abstract

Citation Formats

Methods for producing secreted polypeptides patent, July 2008

High-throughput screening of expressed DNA libraries in filamentous fungi patent-application, August 2003

Production of functional antibodies in filamentous fungi patent-application, January 2004

Methods for degrading lignocellulosic materials patent-application, July 2005

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Strategies for improving heterologous protein production from filamentous fungi journal, January 1994

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patent, July 2008

High-throughput screening of expressed DNA libraries in filamentous fungi
patent-application, August 2003

Production of functional antibodies in filamentous fungi
patent-application, January 2004

Methods for degrading lignocellulosic materials
patent-application, July 2005

Methods of producing mutant polynucleotides
patent-application, January 2006

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patent-application, October 2007

Strategies for improving heterologous protein production from filamentous fungi
journal, January 1994

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