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Title: Isolated fungal promoters and gene transcription terminators and methods of protein and chemical production in a fungus

Abstract

The present invention encompasses isolated gene regulatory elements and gene transcription terminators that are differentially expressed in a native fungus exhibiting a first morphology relative to the native fungus exhibiting a second morphology. The invention also encompasses a method of utilizing a fungus for protein or chemical production. A transformed fungus is produced by transforming a fungus with a recombinant polynucleotide molecule. The recombinant polynucleotide molecule contains an isolated polynucleotide sequence linked operably to another molecule comprising a coding region of a gene of interest. The gene regulatory element and gene transcription terminator may temporally and spatially regulate expression of particular genes for optimum production of compounds of interest in a transgenic fungus.

Inventors:
; ;
Issue Date:
Research Org.:
Pacific Northwest National Lab. (PNNL), Richland, WA (United States)
Sponsoring Org.:
USDOE
OSTI Identifier:
1132775
Patent Number(s):
8,735,562
Application Number:
12/257,261
Assignee:
Battelle Memorial Institute (Richland, WA)
DOE Contract Number:  
AC06-76RL01830
Resource Type:
Patent
Resource Relation:
Patent File Date: 2008 Oct 23
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES

Citation Formats

Dai, Ziyu, Lasure, Linda L, and Magnuson, Jon K. Isolated fungal promoters and gene transcription terminators and methods of protein and chemical production in a fungus. United States: N. p., 2014. Web.
Dai, Ziyu, Lasure, Linda L, & Magnuson, Jon K. Isolated fungal promoters and gene transcription terminators and methods of protein and chemical production in a fungus. United States.
Dai, Ziyu, Lasure, Linda L, and Magnuson, Jon K. Tue . "Isolated fungal promoters and gene transcription terminators and methods of protein and chemical production in a fungus". United States. https://www.osti.gov/servlets/purl/1132775.
@article{osti_1132775,
title = {Isolated fungal promoters and gene transcription terminators and methods of protein and chemical production in a fungus},
author = {Dai, Ziyu and Lasure, Linda L and Magnuson, Jon K},
abstractNote = {The present invention encompasses isolated gene regulatory elements and gene transcription terminators that are differentially expressed in a native fungus exhibiting a first morphology relative to the native fungus exhibiting a second morphology. The invention also encompasses a method of utilizing a fungus for protein or chemical production. A transformed fungus is produced by transforming a fungus with a recombinant polynucleotide molecule. The recombinant polynucleotide molecule contains an isolated polynucleotide sequence linked operably to another molecule comprising a coding region of a gene of interest. The gene regulatory element and gene transcription terminator may temporally and spatially regulate expression of particular genes for optimum production of compounds of interest in a transgenic fungus.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {2014},
month = {5}
}

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Works referenced in this record:

Identification of an essential upstream element in the nopaline synthase promoter by stable and transient assays
journal, August 1987

  • Ebert, P. R.; Ha, S. B.; An, G.
  • Proceedings of the National Academy of Sciences, Vol. 84, Issue 16
  • DOI: 10.1073/pnas.84.16.5745

Identification of Genes Associated with Morphology in Aspergillus niger by Using Suppression Subtractive Hybridization
journal, April 2004