Variant Hypocrea jecorina CBH2 cellulases

Aehle, Wolfgang; Goedegebuur, Frits; Dankmeyer, Lydia; Mitchinson, Colin; Neefe, Paulien; Kelemen, Bradley; Caldwell, Robert; Teunissen, Pauline

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Title: Variant Hypocrea jecorina CBH2 cellulases

Abstract

Described herein are variants of H. jecorina CBH2, a Cel6A enzyme. The present invention provides novel cellobiohydrolases that have altered thermostability.

Inventors:: Aehle, Wolfgang; Goedegebuur, Frits; Dankmeyer, Lydia; Mitchinson, Colin; Neefe, Paulien; Kelemen, Bradley; Caldwell, Robert; Teunissen, Pauline

Issue Date:: 2014-05-06

Research Org.:: National Renewable Energy Laboratory (NREL), Golden, CO (United States)

Sponsoring Org.:: USDOE

OSTI Identifier:: 1130529

Patent Number(s):: 8715997

Application Number:: 13/178,227

Assignee:: Danisco US Inc. (Palo Alto, CA)

Patent Classifications (CPCs):: C - CHEMISTRY C11 - ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES C11D - DETERGENT COMPOSITIONS

C - CHEMISTRY C12 - BIOCHEMISTRY C12N - MICROORGANISMS OR ENZYMES

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C - CHEMISTRY C11 - ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES C11D - DETERGENT COMPOSITIONS
C11D3/38645 - {containing cellulase}

C - CHEMISTRY C12 - BIOCHEMISTRY C12N - MICROORGANISMS OR ENZYMES
C12N9/2437 - {Cellulases

C - CHEMISTRY C12 - BIOCHEMISTRY C12P - FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE {
C12P19/14 - produced by the action of a carbohydrase {

C - CHEMISTRY C12 - BIOCHEMISTRY C12Y - ENZYMES
C12Y302/01091 - Cellulose 1,4-beta-cellobiosidase

Y - NEW / CROSS SECTIONAL TECHNOLOGIES Y02 - TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE Y02E - REDUCTION OF GREENHOUSE GAS [GHG] EMISSIONS, RELATED TO ENERGY GENERATION, TRANSMISSION OR DISTRIBUTION
Y02E50/10 - Biofuels

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DOE Contract Number:: AC36-99GO10337

Resource Type:: Patent

Country of Publication:: United States

Language:: English

Subject:: 59 BASIC BIOLOGICAL SCIENCES

Citation Formats


                    Aehle, Wolfgang, Goedegebuur, Frits, Dankmeyer, Lydia, Mitchinson, Colin, Neefe, Paulien, Kelemen, Bradley, Caldwell, Robert, and Teunissen, Pauline. Variant Hypocrea jecorina CBH2 cellulases.  United States: N. p., 2014. 
        Web.

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                    Aehle, Wolfgang, Goedegebuur, Frits, Dankmeyer, Lydia, Mitchinson, Colin, Neefe, Paulien, Kelemen, Bradley, Caldwell, Robert, & Teunissen, Pauline. Variant Hypocrea jecorina CBH2 cellulases.  United States.

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                    Aehle, Wolfgang, Goedegebuur, Frits, Dankmeyer, Lydia, Mitchinson, Colin, Neefe, Paulien, Kelemen, Bradley, Caldwell, Robert, and Teunissen, Pauline. Tue .  
        "Variant Hypocrea jecorina CBH2 cellulases".  United States.  https://www.osti.gov/servlets/purl/1130529.

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@article{osti_1130529,

  title        = {Variant Hypocrea jecorina CBH2 cellulases},

  author       = {Aehle, Wolfgang and Goedegebuur, Frits and Dankmeyer, Lydia and Mitchinson, Colin and Neefe, Paulien and Kelemen, Bradley and Caldwell, Robert and Teunissen, Pauline},

  abstractNote = {Described herein are variants of H. jecorina CBH2, a Cel6A enzyme. The present invention provides novel cellobiohydrolases that have altered thermostability.},

  doi          = {},

  journal      = {},
number       = ,

  volume       = ,

  place        = {United States},

  year         = {2014},

  month        = {5}

}

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Patent:

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Thomas, Steven; Laymon, Robert A.; Himmel, Michael E.
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Characterization of the secreted cellulases of Trichoderma reesei wild type and mutants during controlled fermentations
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Sheir-Neiss, G.; Montenecourt, B. S.
Applied Microbiology and Biotechnology, Vol. 20, Issue 1, p. 46-53
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A New Model For Enzymatic Hydrolysis of Cellulose Based on the Two-Domain Structure of Cellobiohydrolase I
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Ståhlberg, Jerry; Johansson, Gunnar; Pettersson, Göran
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Aromatic-aromatic interaction: a mechanism of protein structure stabilization
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Burley, S.; Petsko, G.
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Structure of Cellulases and Their Applications
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Ohmiya, Kunio; Sakka, Kazuo; Karita, Shuichi
Biotechnology and Genetic Engineering Reviews, Vol. 14, Issue 1, p. 365-414
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The plasmid replicator AMA1 in Aspergillus nidulans is an inverted duplication of a low-copy-number dispersed genomic repeat
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Aleksenko, Alexei; Clutterbuck, A. John
Molecular Microbiology, Vol. 19, Issue 3, p. 565-574
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Amino acid substitution matrices from protein blocks.
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Henikoff, S.; Henikoff, J. G.
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Protein tolerance to random amino acid change
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Proceedings of the National Academy of Sciences, Vol. 101, Issue 25, p. 9205-9210
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Applications and statistics for multiple high-scoring segments in molecular sequences.
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Karlin, S.; Altschul, S. F.
Proceedings of the National Academy of Sciences, Vol. 90, Issue 12, p. 5873-5877
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Molecular evidence that the asexual industrial fungus Trichoderma reesei is a clonal derivative of the ascomycete Hypocrea jecorina.
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Kuhls, K.; Lieckfeldt, E.; Samuels, G. J.
Proceedings of the National Academy of Sciences, Vol. 93, Issue 15, p. 7755-7760
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A versatile transformation system for the cellulolytic filamentous fungus Trichoderma reesei
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Penttilä, Merja; Nevalainen, Helena; Rättö, Marjaana
Gene, Vol. 61, Issue 2, p. 155-164
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Studies on the chromatographic fractionation of Trichoderma reesei cellulases by hydrophobic interaction
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Tomaz, Cândida T.; Queiroz, João A.
Journal of Chromatography A, Vol. 865, Issue 1-2, p. 123-128
https://doi.org/10.1016/S0021-9673(99)00851-1

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Title: Variant Hypocrea jecorina CBH2 cellulases

Abstract

Citation Formats

Ion-exchange chromatographic purification and quantitative analysis of Trichoderma reesei cellulases cellobiohydrolase I, II and endoglucanase II by fast protein liquid chromatography journal, May 1998

Studies of the cellulolytic system of Trichoderma reesei QM 9414. Analysis of domain function in two cellobiohydrolases by limited proteolysis journal, January 1988

Identification of a fungal cutinase promoter that is inducible by a plant signal via a phosphorylated trans-acting factor. journal, September 1991

Characteristics of fungal cellulases journal, January 1991

The roles and function of cellulose-binding domains journal, September 1997

Cassette mutagenesis: an efficient method for generation of multiple mutations at defined sites journal, January 1985

Regulation of cellulase gene expression in the filamentous fungus Trichoderma reesei. journal, January 1997

Engineering thermostability: lessons from thermophilic proteins journal, January 1995

Separation of endo- and exo-type cellulases using a new affinity chromatography method journal, April 1984

Characterization of cellulases by HPLC separation journal, January 1983

Kinetic parameters and mode of action of the cellobiohydrolases produced by Talaromyces emersonii journal, November 2001

Hydrolysis of cellulose using ternary mixtures of purified celluloses journal, March 1998

Method for producing ascorbic acid intermediates patent, July 2003

Complete nucleotide sequence of a gene coding for Aspergillus aculeatus cellulase (FI-CMCase) journal, January 1990

Secretion of Trichoderma reesei β-glucosidase by Saccharomyces cerevisiae journal, February 1996

Purification and characterization of a β-glucosidase from solid-state cultures of Humicola grisea var. thermoidea journal, January 1996

Transformation of Trichoderma harzianum by high-voltage electric pulse journal, February 1990

A new reaction for colorimetric determination of carbohydrates journal, May 1972

BRAGI: A comprehensive protein modeling program system journal, September 1988

Enzymes, Energy, and the Environment: A Strategic Perspective on the U.S. Department of Energy's Research and Development Activities for Bioethanol journal, October 1999

Detergent cellulase patent, March 1984

Homologous domains in Trichoderma reesei cellulolytic enzymes: Gene sequence and expression of cellobiohydrolase II journal, January 1987

Crystallographic evidence for substrate ring distortion and protein conformational changes during catalysis in cellobiohydrolase Ce16A from Trichoderma reesei journal, September 1999

ACEII, a Novel Transcriptional Activator Involved in Regulation of Cellulase and Xylanase Genes of Trichoderma reesei journal, April 2001

Randomization of genes by PCR mutagenesis. journal, August 1992

Use of Aspergillus overproducing mutants, cured for intergrated plasmid, to overproduce heterologous proteins journal, August 1993

Fractionation of cellulase and β-glucosidase in a Trichoderma reesei culture liquid by use of two-phase partitioning journal, November 1999

Methods for stonewashing fabrics using endoglucanases patent, July 1997

Contribution of hydrophobic interactions to protein stability journal, June 1988

Method for treating cotton-containing fabric with a cellulase composition containing endoglucanase components and which composition is free of exo-cellobiohydrolase I patent, September 1993

Structure and function of Humicola insolens family 6 cellulases: structure of the endoglucanase, Cel6B, at 1.6 Å resolution journal, May 2000

Crystalline cellulose degradation: new insight into the function of cellobiohydrolases journal, May 1997

Properties of cellulolytic enzyme systems journal, April 1985

Detergent compositions containing cellulase compositions deficient in CBH I type components patent, December 2000

Limited proteolysis of the cellobiohydrolase I from Trichoderma reesei: Separation of functional domains journal, August 1986

Isolation of uridine auxotrophs from Trichoderma reesei and efficient transformation with the cloned ura3 and ura5 genes journal, May 1991

W hat G ood I s S ex ? journal, September 1997

The cellulase of Fusarium solani . Purification and specificity of the β-(1→4)-glucanase and the β-d-glucosidase components journal, February 1971

DNA sequence encoding endoglucanase III cellulase patent, December 1995

Biolistic transformation of Trichoderma harzianum and Gliocladium virens using plasmid and genomic DNA journal, October 1993

Molecular Cloning of Exo–Cellobiohydrolase I Derived from Trichoderma Reesei Strain L27 journal, October 1983

Improved transformation efficiency of Aspergillus niger using the homologous niaD gene for nitrate reductase journal, July 1989

Fungal cellulase composition containing alkaline CMC-endoglucanase and essentially no cellobiohydrolase and method of making thereof patent, July 1998

Improved oligonudeotide site-directed rautagenesis using M13 vectors journal, January 1985

[79] Purification of all glycolytic enzymes from one muscle extract journal, January 1982

[57] Rethinking your purification procedure book, January 1990

Simultaneous saccharification and fermentation of lignocellulosic wastes to ethanol using a thermotolerant yeast journal, April 2001

Basic local alignment search tool journal, October 1990

Thermostable purified endoglucanas from acidothermus cellulolyticus ATCC 43068 patent, January 1994

A general method applicable to the search for similarities in the amino acid sequence of two proteins journal, March 1970

Comparison of biosequences journal, December 1981

Orpinomyces cellulase celf protein and coding sequences patent, September 2000

Expression of Aureobasidium pullulans xynA in, and secretion of the xylanase from, Saccharomyces cerevisiae. journal, January 1996

Temperature adaptation of lactate dehydrogenase Structural, functional and genetic aspects journal, February 1988

CBH1 homologs and variant CBH1 cellulases patent, November 2008

Synthesis and secretion of wheat α-amylase in Saccharomyces cerevisiae journal, January 1987

Methods for measuring cellulase activities journal, January 1988

Systems for the mass production of proteins or peptides by microorganisms of the genus humicola patent, June 2002

Variant Hypocrea jecorina CBH2 cellulases patent, August 2011

Cloning and relational analysis of 15 novel fungal endoglucanases from family 12 glycosyl hydrolase journal, May 2002

Efficient secretion of two fungal cellobiohydrolases by Saccharomyces cerevisiae journal, March 1988

Cellulase families and their genes journal, September 1987

Structural Changes of the Active Site Tunnel of Humicola insolens Cellobiohydrolase, Cel6A, upon Oligosaccharide Binding † , ‡ journal, July 1999

Crystal structure of a bacterial family-III cellulose-binding domain: a general mechanism for attachment to cellulose. journal, November 1996

Homologous expression and characterization of Cel61A (EG IV) of Trichoderma reesei journal, December 2001

Trichoderma reesei cellulases and their core domains in the hydrolysis and modification of chemical pulp journal, June 2000

Genetic transformation of intact cells of Bacillus subtilis by electroporation journal, January 1990

Structural and Genetic Analysis of Protein Stability journal, June 1993

Detergent composition for clothing incorporating a cellulase patent, April 1989

Fungal cellulase composition containing alkaline CMC-endoglucanase and essentially no cellobiohydrolase patent, November 1997

Cellulase enzymes and systems for their expressions patent, January 1999

A Rapid Method for the Determination of Diastatic Power journal, January 1984

Construction of mutant and chimeric genes using the polymerase chain reaction journal, January 1989

Antibodies specific for the human retinoblastoma protein identify a family of related polypeptides journal, November 1991

Gene Transfer and Expression book, January 1990

Analytical separation of Trichoderma reesei cellulases by ion-exchange fast protein liquid chromatography journal, January 1987

A simple colorimetric method for the determination of sugars in fruit and vegetables journal, September 1980

Ion-exchange chromatographic purification and quantitative analysis of Trichoderma reesei cellulases cellobiohydrolase I, II and endoglucanase II by fast protein liquid chromatography
journal, May 1998

Studies of the cellulolytic system of Trichoderma reesei QM 9414. Analysis of domain function in two cellobiohydrolases by limited proteolysis
journal, January 1988

Identification of a fungal cutinase promoter that is inducible by a plant signal via a phosphorylated trans-acting factor.
journal, September 1991

Characteristics of fungal cellulases
journal, January 1991

The roles and function of cellulose-binding domains
journal, September 1997

Cassette mutagenesis: an efficient method for generation of multiple mutations at defined sites
journal, January 1985

Regulation of cellulase gene expression in the filamentous fungus Trichoderma reesei.
journal, January 1997

Engineering thermostability: lessons from thermophilic proteins
journal, January 1995

Separation of endo- and exo-type cellulases using a new affinity chromatography method
journal, April 1984

Characterization of cellulases by HPLC separation
journal, January 1983

Kinetic parameters and mode of action of the cellobiohydrolases produced by Talaromyces emersonii
journal, November 2001

Hydrolysis of cellulose using ternary mixtures of purified celluloses
journal, March 1998

Method for producing ascorbic acid intermediates
patent, July 2003

Complete nucleotide sequence of a gene coding for Aspergillus aculeatus cellulase (FI-CMCase)
journal, January 1990

Secretion of Trichoderma reesei β-glucosidase by Saccharomyces cerevisiae
journal, February 1996

Purification and characterization of a β-glucosidase from solid-state cultures of Humicola grisea var. thermoidea
journal, January 1996

Transformation of Trichoderma harzianum by high-voltage electric pulse
journal, February 1990

A new reaction for colorimetric determination of carbohydrates
journal, May 1972

BRAGI: A comprehensive protein modeling program system
journal, September 1988

Enzymes, Energy, and the Environment: A Strategic Perspective on the U.S. Department of Energy's Research and Development Activities for Bioethanol
journal, October 1999

Detergent cellulase
patent, March 1984

Homologous domains in Trichoderma reesei cellulolytic enzymes: Gene sequence and expression of cellobiohydrolase II
journal, January 1987

Crystallographic evidence for substrate ring distortion and protein conformational changes during catalysis in cellobiohydrolase Ce16A from Trichoderma reesei
journal, September 1999

ACEII, a Novel Transcriptional Activator Involved in Regulation of Cellulase and Xylanase Genes of Trichoderma reesei
journal, April 2001

Randomization of genes by PCR mutagenesis.
journal, August 1992

Use of Aspergillus overproducing mutants, cured for intergrated plasmid, to overproduce heterologous proteins
journal, August 1993

Fractionation of cellulase and β-glucosidase in a Trichoderma reesei culture liquid by use of two-phase partitioning
journal, November 1999

Methods for stonewashing fabrics using endoglucanases
patent, July 1997

Contribution of hydrophobic interactions to protein stability
journal, June 1988

Method for treating cotton-containing fabric with a cellulase composition containing endoglucanase components and which composition is free of exo-cellobiohydrolase I
patent, September 1993

Structure and function of Humicola insolens family 6 cellulases: structure of the endoglucanase, Cel6B, at 1.6 Å resolution
journal, May 2000

Crystalline cellulose degradation: new insight into the function of cellobiohydrolases
journal, May 1997

Properties of cellulolytic enzyme systems
journal, April 1985

Detergent compositions containing cellulase compositions deficient in CBH I type components
patent, December 2000

Limited proteolysis of the cellobiohydrolase I from Trichoderma reesei: Separation of functional domains
journal, August 1986

Isolation of uridine auxotrophs from Trichoderma reesei and efficient transformation with the cloned ura3 and ura5 genes
journal, May 1991

W hat G ood I s S ex ?
journal, September 1997

The cellulase of Fusarium solani . Purification and specificity of the β-(1→4)-glucanase and the β-d-glucosidase components
journal, February 1971

DNA sequence encoding endoglucanase III cellulase
patent, December 1995

Biolistic transformation of Trichoderma harzianum and Gliocladium virens using plasmid and genomic DNA
journal, October 1993

Molecular Cloning of Exo–Cellobiohydrolase I Derived from Trichoderma Reesei Strain L27
journal, October 1983

Improved transformation efficiency of Aspergillus niger using the homologous niaD gene for nitrate reductase
journal, July 1989

Fungal cellulase composition containing alkaline CMC-endoglucanase and essentially no cellobiohydrolase and method of making thereof
patent, July 1998

Improved oligonudeotide site-directed rautagenesis using M13 vectors
journal, January 1985

[79] Purification of all glycolytic enzymes from one muscle extract
journal, January 1982

[57] Rethinking your purification procedure
book, January 1990

Simultaneous saccharification and fermentation of lignocellulosic wastes to ethanol using a thermotolerant yeast
journal, April 2001

Basic local alignment search tool
journal, October 1990

Thermostable purified endoglucanas from acidothermus cellulolyticus ATCC 43068
patent, January 1994

A general method applicable to the search for similarities in the amino acid sequence of two proteins
journal, March 1970

Comparison of biosequences
journal, December 1981

Orpinomyces cellulase celf protein and coding sequences
patent, September 2000

Expression of Aureobasidium pullulans xynA in, and secretion of the xylanase from, Saccharomyces cerevisiae.
journal, January 1996

Temperature adaptation of lactate dehydrogenase Structural, functional and genetic aspects
journal, February 1988

CBH1 homologs and variant CBH1 cellulases
patent, November 2008

Synthesis and secretion of wheat α-amylase in Saccharomyces cerevisiae
journal, January 1987

Methods for measuring cellulase activities
journal, January 1988

Systems for the mass production of proteins or peptides by microorganisms of the genus humicola
patent, June 2002

Variant Hypocrea jecorina CBH2 cellulases
patent, August 2011

Cloning and relational analysis of 15 novel fungal endoglucanases from family 12 glycosyl hydrolase
journal, May 2002

Efficient secretion of two fungal cellobiohydrolases by Saccharomyces cerevisiae
journal, March 1988

Cellulase families and their genes
journal, September 1987

Structural Changes of the Active Site Tunnel of Humicola insolens Cellobiohydrolase, Cel6A, upon Oligosaccharide Binding ^† ^, ^‡
journal, July 1999

Crystal structure of a bacterial family-III cellulose-binding domain: a general mechanism for attachment to cellulose.
journal, November 1996

Homologous expression and characterization of Cel61A (EG IV) of Trichoderma reesei
journal, December 2001

Trichoderma reesei cellulases and their core domains in the hydrolysis and modification of chemical pulp
journal, June 2000

Genetic transformation of intact cells of Bacillus subtilis by electroporation
journal, January 1990

Structural and Genetic Analysis of Protein Stability
journal, June 1993

Detergent composition for clothing incorporating a cellulase
patent, April 1989

Fungal cellulase composition containing alkaline CMC-endoglucanase and essentially no cellobiohydrolase
patent, November 1997

Cellulase enzymes and systems for their expressions
patent, January 1999

A Rapid Method for the Determination of Diastatic Power
journal, January 1984

Construction of mutant and chimeric genes using the polymerase chain reaction
journal, January 1989

Antibodies specific for the human retinoblastoma protein identify a family of related polypeptides
journal, November 1991

Gene Transfer and Expression
book, January 1990

Analytical separation of Trichoderma reesei cellulases by ion-exchange fast protein liquid chromatography
journal, January 1987

A simple colorimetric method for the determination of sugars in fruit and vegetables
journal, September 1980

Kinetic characterization of a beta-glucosidase from a yeast, Candida wickerhamii
journal, May 1993

Cloning and Overexpression of the avi2 Gene Encoding a Major Cellulase Produced by Humicola insolens FERM BP-5977
journal, January 2003

Cellulases of Trichoderma reesei
journal, January 1988