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Title: Detection of bioagents using a shear horizontal surface acoustic wave biosensor

Abstract

A biosensor combining the sensitivity of surface acoustic waves (SAW) generated at a frequency of 325 MHz with the specificity provided by antibodies and other ligands for the detection of viral agents. In a preferred embodiment, a lithium tantalate based SAW transducer with silicon dioxide waveguide sensor platform featuring three test and one reference delay lines was used to adsorb antibodies directed against Coxsackie virus B4 or the negative-stranded category A bioagent Sin Nombre virus (SNV). Rapid detection of increasing concentrations of viral particles was linear over a range of order of magnitude for both viruses, and the sensor's selectivity for its target was not compromised by the presence of confounding Herpes Simplex virus type 1 The biosensor was able to delect SNV at doses lower than the load of virus typically found in a human patient suffering from hantavirus cardiopulmonary syndrome (HCPS).

Inventors:
; ; ; ; ; ; ; ;
Issue Date:
Research Org.:
Sandia National Lab. (SNL-NM), Albuquerque, NM (United States)
Sponsoring Org.:
USDOE
OSTI Identifier:
1130065
Patent Number(s):
8709791
Application Number:
12/069,284
Assignee:
STC.UNM (Albuquerque, NM); Sandia National Laboratories (Albuquerque, NM)
Patent Classifications (CPCs):
G - PHYSICS G01 - MEASURING G01N - INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
C - CHEMISTRY C12 - BIOCHEMISTRY C12Q - MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS
DOE Contract Number:  
AC04-94AL85000
Resource Type:
Patent
Resource Relation:
Patent File Date: 2008 Feb 08
Country of Publication:
United States
Language:
English
Subject:
47 OTHER INSTRUMENTATION

Citation Formats

Larson, Richard S, Hjelle, Brian, Hall, Pam R, Brown, David C, Bisoffi, Marco, Brozik, Susan M, Branch, Darren W, Edwards, Thayne L, and Wheeler, David. Detection of bioagents using a shear horizontal surface acoustic wave biosensor. United States: N. p., 2014. Web.
Larson, Richard S, Hjelle, Brian, Hall, Pam R, Brown, David C, Bisoffi, Marco, Brozik, Susan M, Branch, Darren W, Edwards, Thayne L, & Wheeler, David. Detection of bioagents using a shear horizontal surface acoustic wave biosensor. United States.
Larson, Richard S, Hjelle, Brian, Hall, Pam R, Brown, David C, Bisoffi, Marco, Brozik, Susan M, Branch, Darren W, Edwards, Thayne L, and Wheeler, David. Tue . "Detection of bioagents using a shear horizontal surface acoustic wave biosensor". United States. https://www.osti.gov/servlets/purl/1130065.
@article{osti_1130065,
title = {Detection of bioagents using a shear horizontal surface acoustic wave biosensor},
author = {Larson, Richard S and Hjelle, Brian and Hall, Pam R and Brown, David C and Bisoffi, Marco and Brozik, Susan M and Branch, Darren W and Edwards, Thayne L and Wheeler, David},
abstractNote = {A biosensor combining the sensitivity of surface acoustic waves (SAW) generated at a frequency of 325 MHz with the specificity provided by antibodies and other ligands for the detection of viral agents. In a preferred embodiment, a lithium tantalate based SAW transducer with silicon dioxide waveguide sensor platform featuring three test and one reference delay lines was used to adsorb antibodies directed against Coxsackie virus B4 or the negative-stranded category A bioagent Sin Nombre virus (SNV). Rapid detection of increasing concentrations of viral particles was linear over a range of order of magnitude for both viruses, and the sensor's selectivity for its target was not compromised by the presence of confounding Herpes Simplex virus type 1 The biosensor was able to delect SNV at doses lower than the load of virus typically found in a human patient suffering from hantavirus cardiopulmonary syndrome (HCPS).},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {2014},
month = {4}
}

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Works referenced in this record:

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