Materials and methods for efficient succinate and malate production

Jantama, Kaemwich; Haupt, Mark John; Zhang, Xueli; Moore, Jonathan C; Shanmugam, Keelnatham T; Ingram, Lonnie O'Neal

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Title: Materials and methods for efficient succinate and malate production

Abstract

Genetically engineered microorganisms have been constructed to produce succinate and malate in mineral salt media in pH-controlled batch fermentations without the addition of plasmids or foreign genes. The subject invention also provides methods of producing succinate and malate comprising the culture of genetically modified microorganisms.

Inventors:: Jantama, Kaemwich; Haupt, Mark John; Zhang, Xueli; Moore, Jonathan C; Shanmugam, Keelnatham T; Ingram, Lonnie O'Neal

Issue Date:: 2014-04-08

Research Org.:: Univ. of Florida, Gainesville, FL (United States)

Sponsoring Org.:: USDOE

OSTI Identifier:: 1129167

Patent Number(s):: 8691539

Application Number:: 12/529,826

Assignee:: University of Florida Research Foundation, Inc. (Gainesville, FL)

Patent Classifications (CPCs):: C - CHEMISTRY C12 - BIOCHEMISTRY C12N - MICROORGANISMS OR ENZYMES

C - CHEMISTRY C12 - BIOCHEMISTRY C12P - FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE {

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C - CHEMISTRY C12 - BIOCHEMISTRY C12N - MICROORGANISMS OR ENZYMES
C12N15/52 - Genes encoding for enzymes or proenzymes

C - CHEMISTRY C12 - BIOCHEMISTRY C12P - FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE {
C12P7/46 - Dicarboxylic acids having four or less carbon atoms, e.g. fumaric acid, maleic acid

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DOE Contract Number:: FG02-96ER20222; FG36-04GO14019

Resource Type:: Patent

Resource Relation:: Patent File Date: 2008 Mar 19

Country of Publication:: United States

Language:: English

Subject:: 59 BASIC BIOLOGICAL SCIENCES

Citation Formats


                    Jantama, Kaemwich, Haupt, Mark John, Zhang, Xueli, Moore, Jonathan C, Shanmugam, Keelnatham T, and Ingram, Lonnie O'Neal. Materials and methods for efficient succinate and malate production.  United States: N. p., 2014. 
        Web.

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                    Jantama, Kaemwich, Haupt, Mark John, Zhang, Xueli, Moore, Jonathan C, Shanmugam, Keelnatham T, & Ingram, Lonnie O'Neal. Materials and methods for efficient succinate and malate production.  United States.

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                    Jantama, Kaemwich, Haupt, Mark John, Zhang, Xueli, Moore, Jonathan C, Shanmugam, Keelnatham T, and Ingram, Lonnie O'Neal. Tue .  
        "Materials and methods for efficient succinate and malate production".  United States.  https://www.osti.gov/servlets/purl/1129167.

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@article{osti_1129167,

  title        = {Materials and methods for efficient succinate and malate production},

  author       = {Jantama, Kaemwich and Haupt, Mark John and Zhang, Xueli and Moore, Jonathan C and Shanmugam, Keelnatham T and Ingram, Lonnie O'Neal},

  abstractNote = {Genetically engineered microorganisms have been constructed to produce succinate and malate in mineral salt media in pH-controlled batch fermentations without the addition of plasmids or foreign genes. The subject invention also provides methods of producing succinate and malate comprising the culture of genetically modified microorganisms.},

  doi          = {},

  journal      = {},
number       = ,

  volume       = ,

  place        = {United States},

  year         = {2014},

  month        = {4}

}

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Patent:

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Zhang, Xueli; Jantama, Kaemwich; Moore, J. C.
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Method for construction of bacterial strains with increased succinic acid production

Patent Donnelly, Mark I [Warrenville, IL]; Sanville-Millard, Cynthia [Plainfield, IL]; Chatterjee, Ranjini [Park Ridge, IL]

A fermentation process for producing succinic acid is provided comprising selecting a bacterial strain that does not produce succinic acid in high yield, disrupting the normal regulation of sugar metabolism of said bacterial strain, and combining the mutant bacterial strain and selected sugar in anaerobic conditions to facilitate production of succinic acid. Also provided is a method for changing low yield succinic acid producing bacteria to high yield succinic acid producing bacteria comprising selecting a bacterial strain having a phosphotransferase system and altering the phosphotransferase system so as to allow the bacterial strain to simultaneously metabolize different sugars.
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Materials and methods for efficient lactic acid production

Patent Zhou, Shengde; Ingram, Lonnie O&#x27;Neal; Shanmugam, Keelnatham T; ...

The present invention provides derivatives of Escherichia coli constructed for the production of lactic acid. The transformed E. coli of the invention are prepared by deleting the genes that encode competing pathways followed by a growth-based selection for mutants with improved performance. These transformed E. coli are useful for providing an increased supply of lactic acid for use in food and industrial applications.
Full Text Available
Materials and methods for efficient lactic acid production

Patent Zhou, Shengde [Sycamore, IL]; Ingram, Lonnie O'Neal [Gainesville, FL]; Shanmugam, Keelnatham T [Gainesville, FL]; ...

The present invention provides derivatives of ethanologenic Escherichia coli K011 constructed for the production of lactic acid. The transformed E. coli of the invention are prepared by deleting the genes that encode competing pathways followed by a growth-based selection for mutants with improved performance. These transformed E. coli are useful for providing an increased supply of lactic acid for use in food and industrial applications.
Full Text Available
L-malate production by metabolically engineered escherichia coli

Patent Zhang, Xueli; Wang, Xuan; Shanmugam, Keelnatham T.; ...

A process for the production of malic acid in commercially significant quantities from the carbon compounds by genetically modified bacterial strains (GMBS; also referred to as biocatalysts or genetically modified microorganisms) is disclosed. Microorganisms suitable for the production of malic acid can be cultured in one or two-step processes as disclosed herein.
Full Text Available
Engineering the pathway for succinate production

Patent Zhang, Xueli; Jantama, Kaemwich; Moore, Jonathan C.; ...

This invention relates to biocatalysts for the efficient production of succinic acid and/or other products from renewable biological feedstocks. The biocatalysts have a very high efficiency for the growth-coupled production of succinic acid and/or other products from carbohydrate feed stocks as a result of both genetic manipulation and metabolic evolution. More specifically, certain biocatalysts of the present invention produce succinic acid at high titers and yields in mineral salts media during simple pH-controlled batch fermentation without the addition of any exogenous genetic material. The genetic manipulations of the present invention are concerned with energy-conserving strategies coupled with the elimination ofmore » « less
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Title: Materials and methods for efficient succinate and malate production

Abstract

Citation Formats

Recombination activating gene deficient mouse patent, December 1996

Process for making succinic acid, microorganisms for use in the process and methods of obtaining the microorganisms patent, March 1998

Method for the production of dicarboxylic acids patent, February 1999

Method for construction of bacterial strains with increased succinic acid production patent, December 2000

Method for high-density microarray medicated gene expression profiling patent, August 2003

Process for the fermentative preparation of D-pantothenic acid using coryneform bacteria patent, June 2005

Production of chemicals from lignocellulose, biomass or sugars patent, August 2006

Efficient method of preventing growth of microbial genetic transformant after transformation patent, December 2006

Mutant E. coli strain with increased succinic acid production patent, May 2007

Materials and methods for the efficient production of acetate and other products patent-application, August 2004

Aerobic succinate production in bacteria patent-application, August 2005

Materials and methods for efficient lactic acid production patent-application, February 2007

Evolved micro-organisms for the production of 1,2-propanediol patent-application, March 2007

Materials and methods for the efficient production of xylitol patent-application, March 2007

Materials and Methods for the Efficient Production of Acetate and Other Products patent-application, June 2009

Homo-Succinic Acid Producing Microorganism Variant and Process for Preparing Succinic Acid Using the Same patent-application, June 2010

In vitro binding of the response regulator CitB and of its carboxy-terminal domain to A+T-rich DNA target sequences in the control region of the divergent citC and citS operons of Klebsiella pneumoniae 1 1Edited by J. Karn journal, July 1997

Construction of an Escherichia coli K-12 Mutant for Homoethanologenic Fermentation of Glucose or Xylose without Foreign Genes journal, January 2007

Eliminating side products and increasing succinate yields in engineered strains of Escherichia coli C journal, December 2008

Combining metabolic engineering and metabolic evolution to develop nonrecombinant strains of Escherichia coli C that produce succinate and malate journal, January 2008

Determination of the Escherichia coli S -Nitrosoglutathione Response Network Using Integrated Biochemical and Systems Analysis journal, December 2007

Enzymatic Fixation of Carbon Dioxide in -Ketoglutaric Acid journal, November 1948

Ethanol production from hemicellulose hydrolysates of agricultural residues using genetically engineeredEscherichia coli strain KO11 journal, January 1996

A Global Regulatory Role of Gluconeogenic Genes in Escherichia coli Revealed by Transcriptome Network Analysis journal, August 2005

Structure/function studies of phosphoryl transfer by phosphoenolpyruvate carboxykinase journal, March 2004

Development of a metabolic network design and optimization framework incorporating implementation constraints: A succinate production case study journal, January 2006

One-step inactivation of chromosomal genes in Escherichia coli K-12 using PCR products journal, May 2000

Phosphoenolpyruvate carboxylase from Escherichia coli book, January 1969

Engineering Escherichia coli for efficient conversion of glucose to pyruvate journal, February 2004

The regulation of Escherichia coli methylglyoxal synthase; a new control site in glycolysis? journal, March 1971

Methylglyoxal Bypass Identified as Source of Chiral Contamination in l(+) and d(−)-lactate Fermentations by Recombinant Escherichia coli journal, July 2006

Metabolic Analysis of Escherichia coli in the Presence and Absence of the Carboxylating Enzymes Phosphoenolpyruvate Carboxylase and Pyruvate Carboxylase journal, May 2000

Novel keto acid formate-lyase and propionate kinase enzymes are components of an anaerobic pathway in Escherichia coli that degrades L-threonine to propionate journal, February 1998

Succinic acid production from wheat using a biorefining strategy journal, July 2007

Effect of Overexpression of Actinobacillus succinogenes Phosphoenolpyruvate Carboxykinase on Succinate Production in Escherichia coli journal, February 2004

Fermentative Production of Chemicals That Can Be Used for Polymer Synthesis journal, March 2004

Metabolic Engineering of Escherichia coli for Enhanced Production of Succinic Acid, Based on Genome Comparison and In Silico Gene Knockout Simulation journal, December 2005

Genome-Based Metabolic Engineering of Mannheimia succiniciproducens for Succinic Acid Production journal, March 2006

Metabolic engineering of aerobic succinate production systems in Escherichia coli to improve process productivity and achieve the maximum theoretical succinate yield journal, March 2005

A Highly Efficient Escherichia coli-Based Chromosome Engineering System Adapted for Recombinogenic Targeting and Subcloning of BAC DNA journal, April 2001

Chemostat culture characterization of Escherichia coli mutant strains metabolically engineered for aerobic succinate production: A study of the modified metabolic network based on metabolite profile, enzyme activity, and gene expression profile journal, September 2005

Purification and properties of citrate lyase from Escherichia coli journal, September 1983

Low salt medium for lactate and ethanol production by recombinant Escherichia coli B journal, December 2006

A new process for the continuous production of succinic acid from glucose at high yield, titer, and productivity journal, January 2007

Prospects for a bio-based succinate industry journal, July 2007

Global Expression Profiling of Acetate-grown Escherichia coli journal, January 2002

Effect of Different Carbon Sources on the Production of Succinic Acid Using Metabolically Engineered Escherichia coli journal, April 2007

Novel pathway engineering design of the anaerobic central metabolic pathway in Escherichia coli to increase succinate yield and productivity journal, May 2005

Functional Replacement of the Escherichia coliD-(-)-Lactate Dehydrogenase Gene (ldhA) with the L-(+)-Lactate Dehydrogenase Gene (ldhL) from Pediococcus acidilactici journal, April 2003

Effect of carbon sources differing in oxidation state and transport route on succinate production in metabolically engineered Escherichia coli journal, March 2005

13C-metabolic flux analysis of Actinobacillus succinogenes fermentative metabolism at different NaHCO3 and H2 concentrations journal, January 2008

Extracellular melibiose and fructose are intermediates in raffinose catabolism during fermentation to ethanol by engineered enteric bacteria. journal, March 1997

Insights into Actinobacillus succinogenes Fermentative Metabolism in a Chemically Defined Growth Medium journal, November 2005

Reevaluation of citrate lyase from Escherichia coli journal, May 1987

An expanded genome-scale model of Escherichia coli K-12 (iJR904 GSM/GPR) journal, August 2003

EcoCyc: a comprehensive database resource for Escherichia coli journal, December 2004

Production of organic acids by Corynebacterium glutamicum under oxygen deprivation journal, January 2005

Biotechnology of succinic acid production and markets for derived industrial products journal, May 1999

Betaine Tripled the Volumetric Productivity of d(-)-lactate by Escherichia coli Strain SZ132 in Mineral Salts Medium journal, May 2006

Biosynthesis of Glutamate, Aspartate, Asparagine, L-Alanine, and D-Alanine journal, December 2004

C4-Dicarboxylate Degradation in Aerobic and Anaerobic Growth journal, December 2004

A 2-μm DNA-based marker recycling system for multiple gene dispruption in the yeast Saccharomyces cerevisiae journal, March 1999

Batch culture characterization and metabolic flux analysis of succinate-producing Escherichia coli strains journal, May 2006

Efficient Succinic Acid Production from Glucose through Overexpression of Pyruvate Carboxylase in an Escherichia coli Alcohol Dehydrogenase and Lactate Dehydrogenase Mutant journal, January 2005

Enhancement of lactate and succinate formation in adhE or pta-ackA mutants of NADH dehydrogenase-deficient Escherichia coli journal, December 2005

Development of Industrial-Medium-Required Elimination of the 2,3-Butanediol Fermentation Pathway To Maintain Ethanol Yield in an Ethanologenic Strain of Klebsiella oxytoca journal, October 2005

Effects of Growth Mode and Pyruvate Carboxylase on Succinic Acid Production by Metabolically Engineered Strains of Escherichia coli journal, April 2002

Metabolic engineering of Escherichia coli and Corynebacterium glutamicum for biotechnological production of organic acids and amino acids journal, June 2006

Environmental and physiological factors affecting the succinate product ratio during carbohydrate fermentation by Actinobacillus sp. 130Z journal, May 1997

Effects of dissolved CO2 levels on the growth of Mannheimia succiniciproducens and succinic acid production journal, December 2007

Lack of Protective Osmolytes Limits Final Cell Density and Volumetric Productivity of Ethanologenic Escherichia coli KO11 during Xylose Fermentation journal, May 2004

Recombineering: Genetic Engineering in Bacteria Using Homologous Recombination journal, April 2014

The kinetic properties of phosphoenolpyruvate carboxykinase of Escherichia coli journal, April 1980

Engineering the metabolism of Escherichia coli W3110 for the conversion of sugar to redox-neutral and oxidized products: Homoacetate production journal, January 2003

Production of Optically Pure D-Lactic Acid in Mineral Salts Medium by Metabolically Engineered Escherichia coli W3110 journal, January 2003

Multidimensional annotation of the Escherichia coli K-12 genome journal, November 2007

Production of l-alanine by metabolically engineered Escherichia coli journal, September 2007

Recombination activating gene deficient mouse
patent, December 1996

Process for making succinic acid, microorganisms for use in the process and methods of obtaining the microorganisms
patent, March 1998

Method for the production of dicarboxylic acids
patent, February 1999

Method for construction of bacterial strains with increased succinic acid production
patent, December 2000

Method for high-density microarray medicated gene expression profiling
patent, August 2003

Process for the fermentative preparation of D-pantothenic acid using coryneform bacteria
patent, June 2005

Production of chemicals from lignocellulose, biomass or sugars
patent, August 2006

Efficient method of preventing growth of microbial genetic transformant after transformation
patent, December 2006

Mutant E. coli strain with increased succinic acid production
patent, May 2007

Materials and methods for the efficient production of acetate and other products
patent-application, August 2004

Aerobic succinate production in bacteria
patent-application, August 2005

Materials and methods for efficient lactic acid production
patent-application, February 2007

Evolved micro-organisms for the production of 1,2-propanediol
patent-application, March 2007

Materials and methods for the efficient production of xylitol
patent-application, March 2007

Materials and Methods for the Efficient Production of Acetate and Other Products
patent-application, June 2009

Homo-Succinic Acid Producing Microorganism Variant and Process for Preparing Succinic Acid Using the Same
patent-application, June 2010

In vitro binding of the response regulator CitB and of its carboxy-terminal domain to A+T-rich DNA target sequences in the control region of the divergent citC and citS operons of Klebsiella pneumoniae 1 1Edited by J. Karn
journal, July 1997

Construction of an Escherichia coli K-12 Mutant for Homoethanologenic Fermentation of Glucose or Xylose without Foreign Genes
journal, January 2007

Eliminating side products and increasing succinate yields in engineered strains of Escherichia coli C
journal, December 2008

Combining metabolic engineering and metabolic evolution to develop nonrecombinant strains of Escherichia coli C that produce succinate and malate
journal, January 2008

Determination of the Escherichia coli S -Nitrosoglutathione Response Network Using Integrated Biochemical and Systems Analysis
journal, December 2007

Enzymatic Fixation of Carbon Dioxide in -Ketoglutaric Acid
journal, November 1948

Ethanol production from hemicellulose hydrolysates of agricultural residues using genetically engineeredEscherichia coli strain KO11
journal, January 1996

A Global Regulatory Role of Gluconeogenic Genes in Escherichia coli Revealed by Transcriptome Network Analysis
journal, August 2005

Structure/function studies of phosphoryl transfer by phosphoenolpyruvate carboxykinase
journal, March 2004

Development of a metabolic network design and optimization framework incorporating implementation constraints: A succinate production case study
journal, January 2006

One-step inactivation of chromosomal genes in Escherichia coli K-12 using PCR products
journal, May 2000

Phosphoenolpyruvate carboxylase from Escherichia coli
book, January 1969

Engineering Escherichia coli for efficient conversion of glucose to pyruvate
journal, February 2004

The regulation of Escherichia coli methylglyoxal synthase; a new control site in glycolysis?
journal, March 1971

Methylglyoxal Bypass Identified as Source of Chiral Contamination in l(+) and d(−)-lactate Fermentations by Recombinant Escherichia coli
journal, July 2006

Metabolic Analysis of Escherichia coli in the Presence and Absence of the Carboxylating Enzymes Phosphoenolpyruvate Carboxylase and Pyruvate Carboxylase
journal, May 2000

Novel keto acid formate-lyase and propionate kinase enzymes are components of an anaerobic pathway in Escherichia coli that degrades L-threonine to propionate
journal, February 1998

Succinic acid production from wheat using a biorefining strategy
journal, July 2007

Effect of Overexpression of Actinobacillus succinogenes Phosphoenolpyruvate Carboxykinase on Succinate Production in Escherichia coli
journal, February 2004

Fermentative Production of Chemicals That Can Be Used for Polymer Synthesis
journal, March 2004

Metabolic Engineering of Escherichia coli for Enhanced Production of Succinic Acid, Based on Genome Comparison and In Silico Gene Knockout Simulation
journal, December 2005

Genome-Based Metabolic Engineering of Mannheimia succiniciproducens for Succinic Acid Production
journal, March 2006

Metabolic engineering of aerobic succinate production systems in Escherichia coli to improve process productivity and achieve the maximum theoretical succinate yield
journal, March 2005

A Highly Efficient Escherichia coli-Based Chromosome Engineering System Adapted for Recombinogenic Targeting and Subcloning of BAC DNA
journal, April 2001

Chemostat culture characterization of Escherichia coli mutant strains metabolically engineered for aerobic succinate production: A study of the modified metabolic network based on metabolite profile, enzyme activity, and gene expression profile
journal, September 2005

Purification and properties of citrate lyase from Escherichia coli
journal, September 1983

Low salt medium for lactate and ethanol production by recombinant Escherichia coli B
journal, December 2006

A new process for the continuous production of succinic acid from glucose at high yield, titer, and productivity
journal, January 2007

Prospects for a bio-based succinate industry
journal, July 2007

Global Expression Profiling of Acetate-grown Escherichia coli
journal, January 2002

Effect of Different Carbon Sources on the Production of Succinic Acid Using Metabolically Engineered Escherichia coli
journal, April 2007

Novel pathway engineering design of the anaerobic central metabolic pathway in Escherichia coli to increase succinate yield and productivity
journal, May 2005

Functional Replacement of the Escherichia coliD-(-)-Lactate Dehydrogenase Gene (ldhA) with the L-(+)-Lactate Dehydrogenase Gene (ldhL) from Pediococcus acidilactici
journal, April 2003

Effect of carbon sources differing in oxidation state and transport route on succinate production in metabolically engineered Escherichia coli
journal, March 2005

¹³C-metabolic flux analysis of Actinobacillus succinogenes fermentative metabolism at different NaHCO₃ and H₂ concentrations
journal, January 2008

Extracellular melibiose and fructose are intermediates in raffinose catabolism during fermentation to ethanol by engineered enteric bacteria.
journal, March 1997

Insights into Actinobacillus succinogenes Fermentative Metabolism in a Chemically Defined Growth Medium
journal, November 2005

Reevaluation of citrate lyase from Escherichia coli
journal, May 1987

An expanded genome-scale model of Escherichia coli K-12 (iJR904 GSM/GPR)
journal, August 2003

EcoCyc: a comprehensive database resource for Escherichia coli
journal, December 2004

Production of organic acids by Corynebacterium glutamicum under oxygen deprivation
journal, January 2005

Biotechnology of succinic acid production and markets for derived industrial products
journal, May 1999

Betaine Tripled the Volumetric Productivity of d(-)-lactate by Escherichia coli Strain SZ132 in Mineral Salts Medium
journal, May 2006

Biosynthesis of Glutamate, Aspartate, Asparagine, L-Alanine, and D-Alanine
journal, December 2004

C4-Dicarboxylate Degradation in Aerobic and Anaerobic Growth
journal, December 2004

A 2-μm DNA-based marker recycling system for multiple gene dispruption in the yeast Saccharomyces cerevisiae
journal, March 1999

Batch culture characterization and metabolic flux analysis of succinate-producing Escherichia coli strains
journal, May 2006

Efficient Succinic Acid Production from Glucose through Overexpression of Pyruvate Carboxylase in an Escherichia coli Alcohol Dehydrogenase and Lactate Dehydrogenase Mutant
journal, January 2005

Enhancement of lactate and succinate formation in adhE or pta-ackA mutants of NADH dehydrogenase-deficient Escherichia coli
journal, December 2005

Development of Industrial-Medium-Required Elimination of the 2,3-Butanediol Fermentation Pathway To Maintain Ethanol Yield in an Ethanologenic Strain of Klebsiella oxytoca
journal, October 2005

Effects of Growth Mode and Pyruvate Carboxylase on Succinic Acid Production by Metabolically Engineered Strains of Escherichia coli
journal, April 2002

Metabolic engineering of Escherichia coli and Corynebacterium glutamicum for biotechnological production of organic acids and amino acids
journal, June 2006

Environmental and physiological factors affecting the succinate product ratio during carbohydrate fermentation by Actinobacillus sp. 130Z
journal, May 1997

Effects of dissolved CO₂ levels on the growth of Mannheimia succiniciproducens and succinic acid production
journal, December 2007

Lack of Protective Osmolytes Limits Final Cell Density and Volumetric Productivity of Ethanologenic Escherichia coli KO11 during Xylose Fermentation
journal, May 2004

Recombineering: Genetic Engineering in Bacteria Using Homologous Recombination
journal, April 2014

The kinetic properties of phosphoenolpyruvate carboxykinase of Escherichia coli
journal, April 1980

Engineering the metabolism of Escherichia coli W3110 for the conversion of sugar to redox-neutral and oxidized products: Homoacetate production
journal, January 2003

Production of Optically Pure D-Lactic Acid in Mineral Salts Medium by Metabolically Engineered Escherichia coli W3110
journal, January 2003

Multidimensional annotation of the Escherichia coli K-12 genome
journal, November 2007

Production of l-alanine by metabolically engineered Escherichia coli
journal, September 2007