Global to push GA events into
skip to main content

Title: Materials and methods for efficient succinate and malate production

Genetically engineered microorganisms have been constructed to produce succinate and malate in mineral salt media in pH-controlled batch fermentations without the addition of plasmids or foreign genes. The subject invention also provides methods of producing succinate and malate comprising the culture of genetically modified microorganisms.
Inventors:
; ; ; ; ;
Issue Date:
OSTI Identifier:
1129167
Assignee:
University of Florida Research Foundation, Inc. (Gainesville, FL) CHO
Patent Number(s):
8,691,539
Application Number:
12/529,826
Contract Number:
FG02-96ER20222; FG36-04GO14019
Resource Relation:
Patent File Date: 2008 Mar 19
Research Org:
Univ. of Florida, Gainesville, FL (United States)
Sponsoring Org:
USDOE
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES

Works referenced in this record:

Construction of an Escherichia coli K-12 Mutant for Homoethanologenic Fermentation of Glucose or Xylose without Foreign Genes
journal, January 2007
  • Kim, Y.; Ingram, L. O.; Shanmugam, K. T.
  • Applied and Environmental Microbiology, Vol. 73, Issue 6, p. 1766-1771
  • DOI: 10.1128/AEM.02456-06

Combining metabolic engineering and metabolic evolution to develop nonrecombinant strains of Escherichia coli C that produce succinate and malate
journal, January 2008
  • Jantama, Kaemwich; Haupt, M. J.; Svoronos, Spyros A.
  • Biotechnology and Bioengineering, Vol. 99, Issue 5, p. 1140-1153
  • DOI: 10.1002/bit.21694

Structure/function studies of phosphoryl transfer by phosphoenolpyruvate carboxykinase
journal, March 2004
  • Delbaere, Louis T. J.; Sudom, Athena M.; Prasad, Lata
  • Biochimica et Biophysica Acta (BBA) - Proteins and Proteomics, Vol. 1697, Issue 1-2, p. 271-278
  • DOI: 10.1016/j.bbapap.2003.11.030

Development of a metabolic network design and optimization framework incorporating implementation constraints: A succinate production case study
journal, January 2006
  • Cox, Steven J.; Shalel Levanon, Sagit; Sanchez, Ailen
  • Metabolic Engineering, Vol. 8, Issue 1, p. 46-57
  • DOI: 10.1016/j.ymben.2005.09.006

One-step inactivation of chromosomal genes in Escherichia coli K-12 using PCR products
journal, May 2000
  • Datsenko, K. A.; Wanner, B. L.
  • Proceedings of the National Academy of Sciences, Vol. 97, Issue 12, p. 6640-6645
  • DOI: 10.1073/pnas.120163297

Engineering Escherichia coli for efficient conversion of glucose to pyruvate
journal, February 2004
  • Causey, T. B.; Shanmugam, K. T.; Yomano, L. P.
  • Proceedings of the National Academy of Sciences, Vol. 101, Issue 8, p. 2235-2240
  • DOI: 10.1073/pnas.0308171100

The regulation of Escherichia coli methylglyoxal synthase; a new control site in glycolysis?
journal, March 1971

A new process for the continuous production of succinic acid from glucose at high yield, titer, and productivity
journal, January 2007
  • Meynial-Salles, Isabelle; Dorotyn, Sophie; Soucaille, Philippe
  • Biotechnology and Bioengineering, Vol. 99, Issue 1, p. 129-135
  • DOI: 10.1002/bit.21521

Novel pathway engineering design of the anaerobic central metabolic pathway in Escherichia coli to increase succinate yield and productivity
journal, May 2005
  • Sánchez, Ailen M.; Bennett, George N.; San, Ka-Yiu
  • Metabolic Engineering, Vol. 7, Issue 3, p. 229-239
  • DOI: 10.1016/j.ymben.2005.03.001

Functional Replacement of the Escherichia coliD-(-)-Lactate Dehydrogenase Gene (ldhA) with the L-(+)-Lactate Dehydrogenase Gene (ldhL) from Pediococcus acidilactici
journal, April 2003

13C-metabolic flux analysis of Actinobacillus succinogenes fermentative metabolism at different NaHCO3 and H2 concentrations
journal, January 2008

Reevaluation of citrate lyase from Escherichia coli
journal, May 1987
  • Quentmeier, A.; Holzenburg, A.; Mayer, F.
  • Biochimica et Biophysica Acta (BBA) - Protein Structure and Molecular Enzymology, Vol. 913, Issue 1, p. 60-65
  • DOI: 10.1016/0167-4838(87)90232-9

EcoCyc: a comprehensive database resource for Escherichia coli
journal, December 2004
  • Keseler, Ingrid M.; Collado-Vides, Julio; Gama-Castro, Socorro
  • Nucleic Acids Research, Vol. 33, Issue suppl_1, p. D334-D337
  • DOI: 10.1093/nar/gki108

Genetic Changes To Optimize Carbon Partitioning between Ethanol and Biosynthesis in Ethanologenic Escherichia coli
journal, December 2002

A 2-μm DNA-based marker recycling system for multiple gene dispruption in the yeast Saccharomyces cerevisiae
journal, March 1999

Batch culture characterization and metabolic flux analysis of succinate-producing Escherichia coli strains
journal, May 2006
  • Sánchez, Ailen M.; Bennett, George N.; San, Ka-Yiu
  • Metabolic Engineering, Vol. 8, Issue 3, p. 209-226
  • DOI: 10.1016/j.ymben.2005.11.004

Metabolic engineering of Escherichia coli and Corynebacterium glutamicum for biotechnological production of organic acids and amino acids
journal, June 2006
  • Wendisch, Volker F.; Bott, Michael; Eikmanns, Bernhard J.
  • Current Opinion in Microbiology, Vol. 9, Issue 3, p. 268-274
  • DOI: 10.1016/j.mib.2006.03.001

Effects of dissolved CO2 levels on the growth of Mannheimia succiniciproducens and succinic acid production
journal, December 2007
  • Song, Hyohak; Lee, Jeong Wook; Choi, Sol
  • Biotechnology and Bioengineering, Vol. 98, Issue 6, p. 1296-1304
  • DOI: 10.1002/bit.21530

Engineering the metabolism of Escherichia coli W3110 for the conversion of sugar to redox-neutral and oxidized products: Homoacetate production
journal, January 2003
  • Causey, T. B.; Zhou, S.; Shanmugam, K. T.
  • Proceedings of the National Academy of Sciences, Vol. 100, Issue 3, p. 825-832
  • DOI: 10.1073/pnas.0337684100