Title: Nucleic acid encoding a self-assembling split-fluorescent protein system

Abstract

The invention provides a protein labeling and detection system based on self-complementing fragments of fluorescent and chromophoric proteins. The system of the invention is exemplified with various combinations of self-complementing fragments derived from Aequorea victoria Green Fluorescent Protein (GFP), which are used to detect and quantify protein solubility in multiple assay formats, both in vitro and in vivo.

Inventors:

Waldo, Geoffrey S.; Cabantous, Stephanie

Issue Date:

Tue Apr 01 00:00:00 EDT 2014

Research Org.:

Los Alamos National Laboratory (LANL), Los Alamos, NM (United States)

Sponsoring Org.:

USDOE

OSTI Identifier:

1129021

Patent Number(s):

8685667

Application Number:

13/118,174

Assignee:

Los Alamos National Security, LLC (Los Alamos, NM)

Patent Classifications (CPCs):

C - CHEMISTRY C07 - ORGANIC CHEMISTRY C07K - PEPTIDES

C - CHEMISTRY C12 - BIOCHEMISTRY C12N - MICROORGANISMS OR ENZYMES

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C - CHEMISTRY C07 - ORGANIC CHEMISTRY C07K - PEPTIDES

C07K14/43595 - {from coelenteratae, e.g. medusae}
C07K2319/735 - containing a domain for self-assembly, e.g. a viral coat protein

C - CHEMISTRY C12 - BIOCHEMISTRY C12N - MICROORGANISMS OR ENZYMES

C12N15/1058 - {Directional evolution of libraries, e.g. evolution of libraries is achieved by mutagenesis and screening or selection of mixed population of organisms}
C12N15/1065 - {Preparation or screening of tagged libraries, e.g. tagged microorganisms by STM-mutagenesis, tagged polynucleotides, gene tags}

C - CHEMISTRY C12 - BIOCHEMISTRY C12Q - MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS

C12Q1/6897 - involving reporter genes operably linked to promoters

G - PHYSICS G01 - MEASURING G01N - INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES

G01N33/542 - with steric inhibition or signal modification, e.g. fluorescent quenching
G01N33/582 - {with fluorescent label}

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DOE Contract Number:  

FG02-98ER62647; W-7405-ENG-36

Resource Type:

Patent

Resource Relation:

Patent File Date: 2011 Mar 27

Country of Publication:

United States

Language:

English

Subject:

59 BASIC BIOLOGICAL SCIENCES