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Title: Nucleic acid encoding a self-assembling split-fluorescent protein system

Abstract

The invention provides a protein labeling and detection system based on self-complementing fragments of fluorescent and chromophoric proteins. The system of the invention is exemplified with various combinations of self-complementing fragments derived from Aequorea victoria Green Fluorescent Protein (GFP), which are used to detect and quantify protein solubility in multiple assay formats, both in vitro and in vivo.

Inventors:
;
Issue Date:
Research Org.:
Los Alamos National Lab. (LANL), Los Alamos, NM (United States)
Sponsoring Org.:
USDOE
OSTI Identifier:
1129021
Patent Number(s):
8,685,667
Application Number:
13/118,174
Assignee:
Los Alamos National Security, LLC (Los Alamos, NM)
DOE Contract Number:  
FG02-98ER62647; W-7405-ENG-36
Resource Type:
Patent
Resource Relation:
Patent File Date: 2011 Mar 27
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES

Citation Formats

Waldo, Geoffrey S., and Cabantous, Stephanie. Nucleic acid encoding a self-assembling split-fluorescent protein system. United States: N. p., 2014. Web.
Waldo, Geoffrey S., & Cabantous, Stephanie. Nucleic acid encoding a self-assembling split-fluorescent protein system. United States.
Waldo, Geoffrey S., and Cabantous, Stephanie. Tue . "Nucleic acid encoding a self-assembling split-fluorescent protein system". United States. https://www.osti.gov/servlets/purl/1129021.
@article{osti_1129021,
title = {Nucleic acid encoding a self-assembling split-fluorescent protein system},
author = {Waldo, Geoffrey S. and Cabantous, Stephanie},
abstractNote = {The invention provides a protein labeling and detection system based on self-complementing fragments of fluorescent and chromophoric proteins. The system of the invention is exemplified with various combinations of self-complementing fragments derived from Aequorea victoria Green Fluorescent Protein (GFP), which are used to detect and quantify protein solubility in multiple assay formats, both in vitro and in vivo.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {2014},
month = {4}
}

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