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Title: Nucleic acid encoding a self-assembling split-fluorescent protein system

The invention provides a protein labeling and detection system based on self-complementing fragments of fluorescent and chromophoric proteins. The system of the invention is exemplified with various combinations of self-complementing fragments derived from Aequorea victoria Green Fluorescent Protein (GFP), which are used to detect and quantify protein solubility in multiple assay formats, both in vitro and in vivo.
Issue Date:
OSTI Identifier:
Los Alamos National Security, LLC (Los Alamos, NM) LANL
Patent Number(s):
Application Number:
Contract Number:
FG02-98ER62647; W-7405-ENG-36
Resource Relation:
Patent File Date: 2011 Mar 27
Research Org:
Los Alamos National Lab. (LANL), Los Alamos, NM (United States)
Sponsoring Org:
Country of Publication:
United States

Works referenced in this record:

An optical marker based on the UV-induced green-to-red photoconversion of a fluorescent protein
journal, September 2002
  • Ando, R.; Hama, H.; Yamamoto-Hino, M.
  • Proceedings of the National Academy of Sciences, Vol. 99, Issue 20, p. 12651-12656
  • DOI: 10.1073/pnas.202320599

Expansion of the Genetic Code Enables Design of a Novel “Gold” Class of Green Fluorescent Proteins
journal, May 2003

The production, purification and crystallization of a pocilloporin pigment from a reef-forming coral
journal, February 2003
  • Beddoe, Travis; Ling, Michael; Dove, Sophie
  • Acta Crystallographica Section D Biological Crystallography, Vol. 59, Issue 3, p. 597-599
  • DOI: 10.1107/S0907444902023466

A monomeric red fluorescent protein
journal, June 2002
  • Campbell, R. E.; Tour, O.; Palmer, A. E.
  • Proceedings of the National Academy of Sciences, Vol. 99, Issue 12, p. 7877-7882
  • DOI: 10.1073/pnas.082243699

Identification of different emitting species in the red fluorescent protein DsRed by means of ensemble and single-molecule spectroscopy
journal, November 2001
  • Cotlet, M.; Hofkens, J.; Habuchi, S.
  • Proceedings of the National Academy of Sciences, Vol. 98, Issue 25, p. 14398-14403
  • DOI: 10.1073/pnas.251532698

GFP-like chromoproteins as a source of far-red fluorescent proteins
journal, September 2001

A naturally enhanced green fluorescent protein from magnificent sea anemone (Heteractis magnifica) and its functional analysis
journal, February 2003
  • Tu, Hongbin; Xiong, Qian; Zhen, Suilan
  • Biochemical and Biophysical Research Communications, Vol. 301, Issue 4, p. 879-885
  • DOI: 10.1016/S0006-291X(03)00019-6

Visualization of Interactions among bZIP and Rel Family Proteins in Living Cells Using Bimolecular Fluorescence Complementation
journal, April 2002

Circularly permuted green fluorescent proteins engineered to sense Ca2+
journal, March 2001
  • Nagai, T.; Sawano, A.; Park, E. S.
  • Proceedings of the National Academy of Sciences, Vol. 98, Issue 6, p. 3197-3202
  • DOI: 10.1073/pnas.051636098

The Green Fluorescent Protein
journal, June 1998

Genetic screens and directed evolution for protein solubility
journal, February 2003

Rapid protein-folding assay using green fluorescent protein
journal, July 1999
  • Waldo, Geoffrey S.; Standish, Blake M.; Berendzen, Joel
  • Nature Biotechnology, Vol. 17, Issue 7, p. 691-695
  • DOI: 10.1038/10904

Protein–protein interactions monitored in mammalian cells via complementation of β-lactamase enzyme fragments
journal, March 2002
  • Wehrman, Tom; Kleaveland, Benjamin; Her, Jeng-Horng
  • Proceedings of the National Academy of Sciences, Vol. 99, Issue 6, p. 3469-3474
  • DOI: 10.1073/pnas.062043699

A far-red fluorescent protein with fast maturation and reduced oligomerization tendency from Entacmaea quadricolor (Anthozoa, Actinaria)
journal, August 2002
  • Wiedenmann, J.; Schenk, A.; Rocker, C.
  • Proceedings of the National Academy of Sciences, Vol. 99, Issue 18, p. 11646-11651
  • DOI: 10.1073/pnas.182157199

Mutants of Discosoma red fluorescent protein with a GFP-like chromophore
journal, January 2001

Combinatorial Marking of Cells and Organelles with Reconstituted Fluorescent Proteins
journal, October 2004