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Title: Real-time, single-step bioassay using nanoplasmonic resonator with ultra-high sensitivity

Abstract

A nanoplasmonic resonator (NPR) comprising a metallic nanodisk with alternating shielding layer(s), having a tagged biomolecule conjugated or tethered to the surface of the nanoplasmonic resonator for highly sensitive measurement of enzymatic activity. NPRs enhance Raman signals in a highly reproducible manner, enabling fast detection of protease and enzyme activity, such as Prostate Specific Antigen (paPSA), in real-time, at picomolar sensitivity levels. Experiments on extracellular fluid (ECF) from paPSA-positive cells demonstrate specific detection in a complex bio-fluid background in real-time single-step detection in very small sample volumes.

Inventors:
; ; ; ; ;
Issue Date:
Research Org.:
Lawrence Berkeley National Lab. (LBNL), Berkeley, CA (United States)
Sponsoring Org.:
USDOE
OSTI Identifier:
1129019
Patent Number(s):
8685743
Application Number:
12/772,118
Assignee:
The Regents of the University of California (Oakland, CA)
Patent Classifications (CPCs):
C - CHEMISTRY C12 - BIOCHEMISTRY C12Q - MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS
B - PERFORMING OPERATIONS B82 - NANOTECHNOLOGY B82Y - SPECIFIC USES OR APPLICATIONS OF NANOSTRUCTURES
DOE Contract Number:  
AC02-05CH11231
Resource Type:
Patent
Resource Relation:
Patent File Date: 2010 Apr 30
Country of Publication:
United States
Language:
English
Subject:
47 OTHER INSTRUMENTATION

Citation Formats

Zhang, Xiang, Ellman, Jonathan A, Chen, Fanqing Frank, Su, Kai-Hang, Wei, Qi-Huo, and Sun, Cheng. Real-time, single-step bioassay using nanoplasmonic resonator with ultra-high sensitivity. United States: N. p., 2014. Web.
Zhang, Xiang, Ellman, Jonathan A, Chen, Fanqing Frank, Su, Kai-Hang, Wei, Qi-Huo, & Sun, Cheng. Real-time, single-step bioassay using nanoplasmonic resonator with ultra-high sensitivity. United States.
Zhang, Xiang, Ellman, Jonathan A, Chen, Fanqing Frank, Su, Kai-Hang, Wei, Qi-Huo, and Sun, Cheng. Tue . "Real-time, single-step bioassay using nanoplasmonic resonator with ultra-high sensitivity". United States. https://www.osti.gov/servlets/purl/1129019.
@article{osti_1129019,
title = {Real-time, single-step bioassay using nanoplasmonic resonator with ultra-high sensitivity},
author = {Zhang, Xiang and Ellman, Jonathan A and Chen, Fanqing Frank and Su, Kai-Hang and Wei, Qi-Huo and Sun, Cheng},
abstractNote = {A nanoplasmonic resonator (NPR) comprising a metallic nanodisk with alternating shielding layer(s), having a tagged biomolecule conjugated or tethered to the surface of the nanoplasmonic resonator for highly sensitive measurement of enzymatic activity. NPRs enhance Raman signals in a highly reproducible manner, enabling fast detection of protease and enzyme activity, such as Prostate Specific Antigen (paPSA), in real-time, at picomolar sensitivity levels. Experiments on extracellular fluid (ECF) from paPSA-positive cells demonstrate specific detection in a complex bio-fluid background in real-time single-step detection in very small sample volumes.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {2014},
month = {4}
}

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Works referenced in this record:

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