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Title: Real-time, single-step bioassay using nanoplasmonic resonator with ultra-high sensitivity

A nanoplasmonic resonator (NPR) comprising a metallic nanodisk with alternating shielding layer(s), having a tagged biomolecule conjugated or tethered to the surface of the nanoplasmonic resonator for highly sensitive measurement of enzymatic activity. NPRs enhance Raman signals in a highly reproducible manner, enabling fast detection of protease and enzyme activity, such as Prostate Specific Antigen (paPSA), in real-time, at picomolar sensitivity levels. Experiments on extracellular fluid (ECF) from paPSA-positive cells demonstrate specific detection in a complex bio-fluid background in real-time single-step detection in very small sample volumes.
Inventors:
; ; ; ; ;
Issue Date:
OSTI Identifier:
1129019
Assignee:
The Regents of the University of California (Oakland, CA) LBNL
Patent Number(s):
8,685,743
Application Number:
12/772,118
Contract Number:
AC02-05CH11231
Resource Relation:
Patent File Date: 2010 Apr 30
Research Org:
Lawrence Berkeley National Lab. (LBNL), Berkeley, CA (United States)
Sponsoring Org:
USDOE
Country of Publication:
United States
Language:
English
Subject:
47 OTHER INSTRUMENTATION

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