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Title: Variant Hypocrea jecorina CBH1 cellulase

Described herein are variants of H. jecorina CBH I, a Cel7 enzyme. The present invention provides novel cellobiohydrolases that have improved thermostability and reversibility.
Inventors:
; ; ; ; ; ; ;
Issue Date:
OSTI Identifier:
1128547
Assignee:
Danisco US Inc. (Palo Alto, CA) NREL
Patent Number(s):
8,679,817
Application Number:
13/523,800
Contract Number:
AC36-99GO10337
Research Org:
National Renewable Energy Lab. (NREL), Golden, CO (United States)
Sponsoring Org:
USDOE
Country of Publication:
United States
Language:
English
Subject:
60 APPLIED LIFE SCIENCES

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Recombinant immunoglobin preparations
patent, March 1989

Detergent composition for clothing incorporating a cellulase
patent, April 1989

Method for treating cotton-containing fabric with a cellulase composition containing endoglucanase components and which composition is free of exo-cellobiohydrolase I
patent, September 1993

DNA sequence encoding endoglucanase III cellulase
patent, December 1995

Methods for reducing the harshness of a cotton-containing fabric
patent, July 1997

Fungal cellulase composition containing alkaline CMC-endoglucanase and essentially no cellobiohydrolase and method of making thereof
patent, July 1998

Detergent compositions containing cellulase compositions deficient in CBH I type components
patent, December 2000

Cellobiohydrolase I gene and improved variants
patent, May 2008

Basic local alignment search tool
journal, October 1990
  • Altschul, Stephen F.; Gish, Warren; Miller, Webb
  • Journal of Molecular Biology, Vol. 215, Issue 3, p. 403-410
  • DOI: 10.1016/S0022-2836(05)80360-2

ACEII, a Novel Transcriptional Activator Involved in Regulation of Cellulase and Xylanase Genes of Trichoderma reesei
journal, April 2001
  • Aro, Nina; Saloheimo, Anu; Ilmén, Marja
  • Journal of Biological Chemistry, Vol. 276, Issue 26, p. 24309-24314
  • DOI: 10.1074/jbc.M003624200

Transformation of Aspergillus nidulans by the orotidine-5′-phosphate decarboxylase gene of Neurospora crassa
journal, April 1983
  • Ballance, D. J.; Buxton, F. P.; Turner, G.
  • Biochemical and Biophysical Research Communications, Vol. 112, Issue 1, p. 284-289
  • DOI: 10.1016/0006-291X(83)91828-4

Engineering of a glycosidase Family 7 cellobiohydrolase to more alkaline pH optimum: the pH behaviour of Trichoderma reesei Cel7A and its E223S/ A224H/L225V/T226A/D262G mutant
journal, May 2001
  • Becker, Dieter; Braet, Christophe; Brumer, Harry
  • Biochemical Journal, Vol. 356, Issue 1, p. 19-30
  • DOI: 10.1042/bj3560019

Isolation of uridine auxotrophs from Trichoderma reesei and efficient transformation with the cloned ura3 and ura5 genes
journal, May 1991
  • Bergès, Thierry; Barreau, Christian
  • Current Genetics, Vol. 19, Issue 5, p. 359-365
  • DOI: 10.1007/BF00309596

Fractionation of cellulase and β-glucosidase in a Trichoderma reesei culture liquid by use of two-phase partitioning
journal, November 1999
  • Brumbauer, Anikó; Johansson, Göte; Réczey, Kati
  • Bioseparation, Vol. 7, Issue 6, p. 287-295
  • DOI: 10.1023/A:1008153418019

Improved transformation efficiency of Aspergillus niger using the homologous niaD gene for nitrate reductase
journal, July 1989
  • Campbell, Edward I.; Unkles, Shiela E.; Macro, Janet A.
  • Current Genetics, Vol. 16, Issue 1, p. 53-56
  • DOI: 10.1007/BF00411084

Oligosaccharide specificity of a family 7 endoglucanase: insertion of potential sugar-binding subsites
journal, September 1997
  • Davies, Gideon J.; Ducros, Valérie; Lewis, Richard J.
  • Journal of Biotechnology, Vol. 57, Issue 1-3, p. 91-100
  • DOI: 10.1016/S0168-1656(97)00092-8

Chapter 43 Rethinking Your Purification Procedure
book, January 2009

Analytical separation of Trichoderma reesei cellulases by ion-exchange fast protein liquid chromatography
journal, January 1987

Purification and characterization of a β-glucosidase from solid-state cultures of Humicola grisea var. thermoidea
journal, January 1996
  • Filho, Edivaldo Ximenes Ferreira
  • Canadian Journal of Microbiology, Vol. 42, Issue 1, p. 1-5
  • DOI: 10.1139/m96-001

Characterization of cellulases by HPLC separation
journal, January 1983
  • Fliess, A.; Schügerl, K.
  • European Journal of Applied Microbiology and Biotechnology, Vol. 17, Issue 5, p. 314-318
  • DOI: 10.1007/BF00508028

Urea and Thermal Equilibrium Denaturation Studies on the Dimerization Domain of Escherichia coli Trp Repressor
journal, May 1997
  • Gloss, Lisa M.; Matthews, C. Robert
  • Biochemistry, Vol. 36, Issue 19, p. 5612-5623
  • DOI: 10.1021/bi970056e

Cloning and relational analysis of 15 novel fungal endoglucanases from family 12 glycosyl hydrolase
journal, May 2002
  • Goedegebuur, Frits; Fowler, Timothy; Phillips, Jay
  • Current Genetics, Vol. 41, Issue 2, p. 89-98
  • DOI: 10.1007/s00294-002-0290-2

Characteristics of fungal cellulases
journal, January 1991

Cloning, sequencing and overexpression of a Rhodothermus marinus gene encoding a thermostable cellulase of glycosyl hydrolase family 12
journal, March 1998
  • Halldórsdóttir, S.; Thórólfsdót, E. T.; Spilliaert, R.
  • Applied Microbiology and Biotechnology, Vol. 49, Issue 3, p. 277-284
  • DOI: 10.1007/s002530051169

Antibodies specific for the human retinoblastoma protein identify a family of related polypeptides.
journal, November 1991
  • Hu, Q. J.; Bautista, C.; Edwards, G. M.
  • Molecular and Cellular Biology, Vol. 11, Issue 11, p. 5792-5799
  • DOI: 10.1128/MCB.11.11.5792

Cloning and sequencing of the cDNA encoding β-glucosidase 1 from Aspergillus aculeatus
journal, September 1996

Cellulase families and their genes
journal, September 1987
  • Knowles, Jonathan; Lehtovaara, Päivi; Teeri, Tuula
  • Trends in Biotechnology, Vol. 5, Issue 9, p. 255-261
  • DOI: 10.1016/0167-7799(87)90102-8

Continuous cultures of fused cells secreting antibody of predefined specificity
journal, August 1975
  • Köhler, G.; Milstein, C.
  • Nature, Vol. 256, Issue 5517, p. 495-497
  • DOI: 10.1038/256495a0

Simultaneous saccharification and fermentation of lignocellulosic wastes to ethanol using a thermotolerant yeast
journal, April 2001

Conjugative transfer of a shuttle plasmid for Escherichia coli to Mycobacterium smegmatis
journal, May 1990

The roles and function of cellulose-binding domains
journal, September 1997

Ion-exchange chromatographic purification and quantitative analysis of Trichoderma reesei cellulases cellobiohydrolase I, II and endoglucanase II by fast protein liquid chromatography
journal, May 1998

Site-directed mutagenesis of the putative catalytic residues of Trichoderma reesci cellobiohydrolase I and endoglucanase I
journal, November 1990
  • Mitsuishi, Yasushi; Nitisinprasert, Sunee; Saloheimo, Markku
  • FEBS Letters, Vol. 275, Issue 1-2, p. 135-138
  • DOI: 10.1016/0014-5793(90)81457-Y

A general method applicable to the search for similarities in the amino acid sequence of two proteins
journal, March 1970

Molecular Biology of Cellulolytic Fungi
book, January 1995

Specific quantification of trichoderma reesei cellulases in reconstituted mixtures and its application to cellulase-cellulose binding studies
journal, October 1994
  • Nidetzky, Bernd; Claeyssens, Marc
  • Biotechnology and Bioengineering, Vol. 44, Issue 8, p. 961-966
  • DOI: 10.1002/bit.260440812

A versatile transformation system for the cellulolytic filamentous fungus Trichoderma reesei
journal, January 1987

Efficient secretion of two fungal cellobiohydrolases by Saccharomyces cerevisiae
journal, March 1988

Synthesis and secretion of wheat α-amylase in Saccharomyces cerevisiae
journal, January 1987

Engineering thermostability: lessons from thermophilic proteins
journal, January 1995

CelS: a novel endoglucanase identified from Erwinia carotovora subsp. carotovora
journal, May 1990

Cloning and sequencing of cellulase cDNA from Aspergillus kawachii and its expression in Saccharomyces cerevisiae
journal, April 1995
  • Sakamoto, S.; Tamura, G.; Ito, K.
  • Current Genetics, Vol. 27, Issue 5, p. 435-439
  • DOI: 10.1007/BF00311212

EGIII, a new endoglucanase from Trichoderma reesei: the characterization of both gene and enzyme
journal, March 1988

Cellulases of Trichoderma reesei
journal, January 1988

[79] Purification of all glycolytic enzymes from one muscle extract
journal, January 1982

Characterization of the secreted cellulases of Trichoderma reesei wild type and mutants during controlled fermentations
journal, July 1984
  • Sheir-Neiss, G.; Montenecourt, B. S.
  • Applied Microbiology and Biotechnology, Vol. 20, Issue 1, p. 46-53
  • DOI: 10.1007/BF00254645

Enzymic activities of endo-1,4-β-d-glucanases purified from Trichoderma viride
journal, March 1978
  • Shoemaker, S. P.; Brown, R. D.
  • Biochimica et Biophysica Acta (BBA) - Enzymology, Vol. 523, Issue 1, p. 133-146
  • DOI: 10.1016/0005-2744(78)90016-5

Molecular Cloning of Exo–Cellobiohydrolase I Derived from Trichoderma Reesei Strain L27
journal, October 1983
  • Shoemaker, S.; Schweickart, V.; Ladner, M.
  • Nature Biotechnology, Vol. 1, Issue 8, p. 691-696
  • DOI: 10.1038/nbt1083-691

Comparison of biosequences
journal, December 1981

Cloning and Sequencing of a Rhodothermus marinus Gene, bglA, Coding for a Thermostable beta-Glucanase and its Expression in Escherichia coli
journal, September 1994
  • Spilliaert, Remi; Hreggvidsson, Gudmundur Oli; Kristjansson, Jakob Kristjan
  • European Journal of Biochemistry, Vol. 224, Issue 3, p. 923-930
  • DOI: 10.1111/j.1432-1033.1994.00923.x

Trichoderma reesei cellobiohydrolase I with an endoglucanase cellulose-binding domain: action on bacterial microcrystalline cellulose
journal, September 1997

A New Model For Enzymatic Hydrolysis of Cellulose Based on the Two-Domain Structure of Cellobiohydrolase I
journal, March 1991
  • Ståhlberg, Jerry; Johansson, Gunnar; Pettersson, Göran
  • Nature Biotechnology, Vol. 9, Issue 3, p. 286-290
  • DOI: 10.1038/nbt0391-286

Trichoderma reesei cellulases and their core domains in the hydrolysis and modification of chemical pulp
journal, June 2000

Crystalline cellulose degradation: new insight into the function of cellobiohydrolases
journal, May 1997

Homologous domains in Trichoderma reesei cellulolytic enzymes: Gene sequence and expression of cellobiohydrolase II
journal, January 1987

Codon optimization of xylanase gene xynB from the thermophilic bacterium Dictyoglomus thermophilum for expression in the filamentous fungus Trichoderma reesei
journal, September 2000

Studies on the chromatographic fractionation of Trichoderma reesei cellulases by hydrophobic interaction
journal, December 1999

Studies of the cellulolytic system of Trichoderma reesei QM 9414. Analysis of domain function in two cellobiohydrolases by limited proteolysis
journal, January 1988

Development of a homologous transformation system for Aspergillus niger based on the pyrG gene
journal, January 1987
  • van Hartingsveldt, Wim; Mattern, Ineke E.; van Zeijl, Cora M. J.
  • Molecular and General Genetics MGG, Vol. 206, Issue 1, p. 71-75
  • DOI: 10.1007/BF00326538

Separation of endo- and exo-type cellulases using a new affinity chromatography method
journal, April 1984
  • van Tilbeurgh, Herman; Bhikhabhai, Ramagauri; Göran Pettersson, L.
  • FEBS Letters, Vol. 169, Issue 2, p. 215-218
  • DOI: 10.1016/0014-5793(84)80321-X

Use of Aspergillus overproducing mutants, cured for intergrated plasmid, to overproduce heterologous proteins
journal, August 1993
  • Ward, Michael; Wilson, Lori J.; Kodama, Katherine H.
  • Applied Microbiology and Biotechnology, Vol. 39, Issue 6, p. 738-743
  • DOI: 10.1007/BF00164459

Cassette mutagenesis: an efficient method for generation of multiple mutations at defined sites
journal, January 1985

Methods for measuring cellulase activities
journal, January 1988

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