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Title: ADS genes for reducing saturated fatty acid levels in seed oils

Abstract

The present invention relates to enzymes involved in lipid metabolism. In particular, the present invention provides coding sequences for Arabidopsis Desaturases (ADS), the encoded ADS polypeptides, and methods for using the sequences and encoded polypeptides, where such methods include decreasing and increasing saturated fatty acid content in plant seed oils.

Inventors:
;
Issue Date:
Research Org.:
BNL (Brookhaven National Laboratory (BNL), Upton, NY (United States))
Sponsoring Org.:
USDOE
OSTI Identifier:
1127126
Patent Number(s):
8,674,176
Application Number:
12/686,975
Assignee:
BNL
DOE Contract Number:  
AC02-98CH10886
Resource Type:
Patent
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES

Citation Formats

Heilmann, Ingo H, and Shanklin, John. ADS genes for reducing saturated fatty acid levels in seed oils. United States: N. p., 2014. Web.
Heilmann, Ingo H, & Shanklin, John. ADS genes for reducing saturated fatty acid levels in seed oils. United States.
Heilmann, Ingo H, and Shanklin, John. Tue . "ADS genes for reducing saturated fatty acid levels in seed oils". United States. https://www.osti.gov/servlets/purl/1127126.
@article{osti_1127126,
title = {ADS genes for reducing saturated fatty acid levels in seed oils},
author = {Heilmann, Ingo H and Shanklin, John},
abstractNote = {The present invention relates to enzymes involved in lipid metabolism. In particular, the present invention provides coding sequences for Arabidopsis Desaturases (ADS), the encoded ADS polypeptides, and methods for using the sequences and encoded polypeptides, where such methods include decreasing and increasing saturated fatty acid content in plant seed oils.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {2014},
month = {3}
}

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Works referenced in this record:

Two FAD3 Desaturase Genes Control the Level of Linolenic Acid in Flax Seed
journal, August 2005

  • Vrinten, Patricia; Hu, Zhiyuan; Munchinsky, Mary-Ann
  • Plant Physiology, Vol. 139, Issue 1
  • DOI: 10.1104/pp.105.064451

Protein annotation: detective work for function prediction
journal, June 1998


Go hunting in sequence databases but watch out for the traps
journal, October 1996


Efficient functioning of plant promoters and poly(A) sites in Xenopus oocytes
journal, January 1989

  • Ballas, Nurit; Broido, Shimshon; Soreq, Hermona
  • Nucleic Acids Research, Vol. 17, Issue 19
  • DOI: 10.1093/nar/17.19.7891

Exploring the Hydroxylation−Dehydrogenation Connection:  Novel Catalytic Activity of Castor Stearoyl-ACP Δ 9 Desaturase
journal, April 2002

  • Behrouzian, Behnaz; Savile, Christopher K.; Dawson, Brian
  • Journal of the American Chemical Society, Vol. 124, Issue 13
  • DOI: 10.1021/ja012252l

Processing of the initiation methionine from proteins: properties of the Escherichia coli methionine aminopeptidase and its gene structure.
journal, February 1987


A chimaeric antibiotic resistance gene as a selectable marker for plant cell transformation
journal, July 1983

  • Bevan, Michael W.; Flavell, Richard B.; Chilton, Mary-Dell
  • Nature, Vol. 304, Issue 5922
  • DOI: 10.1038/304184a0

A Rapid Method of Total Lipid Extraction and Purification
journal, January 1959

  • Bligh, E. G.; Dyer, W. J.
  • Canadian Journal of Biochemistry and Physiology, Vol. 37, Issue 1
  • DOI: 10.1139/y59-099

Hygromycin B phosphotransferase as a selectable marker for DNA transfer experiments with higher eucaryotic cells.
journal, December 1984

  • Blochlinger, K.; Diggelmann, H.
  • Molecular and Cellular Biology, Vol. 4, Issue 12
  • DOI: 10.1128/MCB.4.12.2929

A bifunctional oleate 12‐hydroxylase: desaturase from Lesquerella fendleri
journal, January 1998


Catalytic Plasticity of Fatty Acid Modification Enzymes Underlying Chemical Diversity of Plant Lipids
journal, November 1998


Trialkylsulfonium- and Trialkylselenoniumhydroxides for the Pyrolytic Alkylation of Acidic Compounds
journal, January 1982


Randomization of genes by PCR mutagenesis.
journal, August 1992

  • Cadwell, R. C.; Joyce, G. F.
  • Genome Research, Vol. 2, Issue 1, p. 28-33
  • DOI: 10.1101/gr.2.1.28

Introns increase gene expression in cultured maize cells.
journal, December 1987

  • Callis, J.; Fromm, M.; Walbot, V.
  • Genes & Development, Vol. 1, Issue 10, p. 1183-1200
  • DOI: 10.1101/gad.1.10.1183

Organ-specific modulation of gene expression in transgenic plants using antisene RNA
journal, July 1990

  • Cannon, Maura; Platz, Jerry; O'Leary, Maureen
  • Plant Molecular Biology, Vol. 15, Issue 1
  • DOI: 10.1007/BF00017722

New RNA Polymerase from Escherichia coli infected with Bacteriophage T7
journal, October 1970

  • Chamberlin, Michael; McGrath, Janet; Waskell, Lucy
  • Nature, Vol. 228, Issue 5268, p. 227-231
  • DOI: 10.1038/228227a0

Regulatory Elements That Control Transcription Activation and Unsaturated Fatty Acid-mediated Repression of the Saccharomyces cerevisiae OLE1 Gene
journal, February 1996

  • Choi, Jae-Yeon; Stukey, Joseph; Hwang, Sue-Yun
  • Journal of Biological Chemistry, Vol. 271, Issue 7
  • DOI: 10.1074/jbc.271.7.3581

Stable Transformation of Soybean Callus by DNA-Coated Gold Particles
journal, July 1988

  • Christou, Paul; McCabe, Dennis E.; Swain, William F.
  • Plant Physiology, Vol. 87, Issue 3
  • DOI: 10.1104/pp.87.3.671

Floral dip: a simplified method for Agrobacterium-mediated transformation of Arabidopsis thaliana
journal, December 1998


Molecular evolution of an arsenate detoxification pathway by DNA shuffling
journal, May 1997

  • Crameri, Andreas; Dawes, Glenn; Rodriguez, Emilio
  • Nature Biotechnology, Vol. 15, Issue 5
  • DOI: 10.1038/nbt0597-436

Improved Green Fluorescent Protein by Molecular Evolution Using DNA Shuffling
journal, March 1996

  • Crameri, Andreas; Whitehorn, Erik A.; Tate, Emily
  • Nature Biotechnology, Vol. 14, Issue 3
  • DOI: 10.1038/nbt0396-315

Synthesis of oligonucleotides on cellulose by a phosphotriester method
journal, January 1980


Integration of foreign DNA following microinjection of tobacco mesophyll protoplasts
journal, February 1986

  • Crossway, Anne; Oakes, Janette V.; Irvine, Jonathan M.
  • MGG Molecular & General Genetics, Vol. 202, Issue 2
  • DOI: 10.1007/BF00331634

Peptides on phage: a vast library of peptides for identifying ligands
journal, August 1990

  • Cwirla, S. E.; Peters, E. A.; Barrett, R. W.
  • Proceedings of the National Academy of Sciences, Vol. 87, Issue 16, p. 6378-6382
  • DOI: 10.1073/pnas.87.16.6378

Genetically Engineered Fertile Indica-Rice Recovered from Protoplasts
journal, August 1990

  • Datta, Swapan K.; Peterhans, Alex; Datta, Karabi
  • Nature Biotechnology, Vol. 8, Issue 8
  • DOI: 10.1038/nbt0890-736

Acyl Carriers Used as Substrates by the Desaturases and Elongases Involved in Very Long-chain Polyunsaturated Fatty Acids Biosynthesis Reconstituted in Yeast
journal, June 2003

  • Domergue, Frédéric; Abbadi, Amine; Ott, Claudia
  • Journal of Biological Chemistry, Vol. 278, Issue 37
  • DOI: 10.1074/jbc.M305990200

Overlapping Destinations for Two Dual Targeted Glycyl-tRNA Synthetases in Arabidopsis thaliana and Phaseolus vulgaris
journal, February 2001

  • Duchêne, Anne-Marie; Peeters, Nemo; Dietrich, André
  • Journal of Biological Chemistry, Vol. 276, Issue 18
  • DOI: 10.1074/jbc.M011525200

Molecular Analysis of a Bifunctional Fatty Acid Conjugase/Desaturase from Tung. Implications for the Evolution of Plant Fatty Acid Diversity
journal, November 2002

  • Dyer, John M.; Chapital, Dorselyn C.; Kuan, Jui-Chang W.
  • Plant Physiology, Vol. 130, Issue 4
  • DOI: 10.1104/pp.102.010835

DNA polymerase fidelity and the polymerase chain reaction.
journal, August 1991

  • Eckert, K. A.; Kunkel, T. A.
  • Genome Research, Vol. 1, Issue 1
  • DOI: 10.1101/gr.1.1.17

Predicting Subcellular Localization of Proteins Based on their N-terminal Amino Acid Sequence
journal, July 2000

  • Emanuelsson, Olof; Nielsen, Henrik; Brunak, Søren
  • Journal of Molecular Biology, Vol. 300, Issue 4
  • DOI: 10.1006/jmbi.2000.3903

The Suitability of Lipase from Rhizopus arrhizus delemar for Analysis of Fatty Acid Distribution in Dihexosyl Diglycerides, Phospholipids and Plant Sulfolipids
journal, January 1973

  • Fischer, Werner; Heinz, Ernst; Zeus, Manfred
  • Hoppe-Seyler´s Zeitschrift für physiologische Chemie, Vol. 354, Issue 2
  • DOI: 10.1515/bchm2.1973.354.2.1115

Inheritance and Expression of Chimeric Genes in the Progeny of Transgenic Maize Plants
journal, September 1990

  • Fromm, Michael E.; Morrish, Fionnuala; Armstrong, Charles
  • Nature Biotechnology, Vol. 8, Issue 9
  • DOI: 10.1038/nbt0990-833

Characterization of  A9 Acyl-lipid Desaturase Homologues from Arabidopsis thaliana
journal, February 1998


Isolation of a ubiquitin-ribosomal protein gene (ubi3) from potato and expression of its promoter in transgenic plants
journal, January 1994

  • Garbarino, Joan E.; Belknap, William R.
  • Plant Molecular Biology, Vol. 24, Issue 1
  • DOI: 10.1007/BF00040579

Use of plant roots for phytoremediation and molecular farming
journal, May 1999

  • Gleba, D.; Borisjuk, N. V.; Borisjuk, L. G.
  • Proceedings of the National Academy of Sciences, Vol. 96, Issue 11
  • DOI: 10.1073/pnas.96.11.5973

SV40-transformed simian cells support the replication of early SV40 mutants
journal, January 1981


Effect of deletions in the cauliflower mosaic virus polyadenylation sequence on the choice of the polyadenylation sites in tobacco protoplasts
journal, April 1991

  • Guerineau, Fran�ois; Brooks, Louise; Mullineaux, Philip
  • MGG Molecular & General Genetics, Vol. 226-226, Issue 1-2
  • DOI: 10.1007/BF00273597

DGD1-independent biosynthesis of extraplastidic galactolipids after phosphate deprivation in Arabidopsis
journal, September 2000

  • Hartel, H.; Dormann, P.; Benning, C.
  • Proceedings of the National Academy of Sciences, Vol. 97, Issue 19, p. 10649-10654
  • DOI: 10.1073/pnas.180320497

Simple RNA enzymes with new and highly specific endoribonuclease activities
journal, August 1988

  • Haseloff, Jim; Gerlach, Wayne L.
  • Nature, Vol. 334, Issue 6183
  • DOI: 10.1038/334585a0

A Polyethylene Glycol-Mediated Protoplast Transformation System for Production of Fertile Transgenic Rice Plants
journal, July 1990

  • Hayashimoto, Akio; Li, Zhijian; Murai, Norimoto
  • Plant Physiology, Vol. 93, Issue 3
  • DOI: 10.1104/pp.93.3.857

Biolistic introduction of a synthetic Bt gene into elite maize
journal, February 1995


Production of Transgenic Soybean Plants Using Agrobacterium-Mediated DNA Transfer
journal, August 1988

  • Hinchee, Maud A. W.; Connor-Ward, Dannette V.; Newell, Christine A.
  • Nature Biotechnology, Vol. 6, Issue 8
  • DOI: 10.1038/nbt0888-915

New metal chelate adsorbent selective for proteins and peptides containing neighbouring histidine residues
journal, January 1987


Generation of a large combinatorial library of the immunoglobulin repertoire in phage lambda
journal, December 1989


Solid phase synthesis of polynucleotides. VIII. Synthesis of mixed oligodeoxyribonucleotides by the phosphotriester solid phase method 1
journal, January 1983

  • Ike, Yoshimasa; Ikuta, Satoshi; Sato, Moriyuki
  • Nucleic Acids Research, Vol. 11, Issue 2
  • DOI: 10.1093/nar/11.2.477

High efficiency transformation of maize (Zea mays L.) mediated by Agrobacterium tumefaciens
journal, June 1996

  • Ishida, Yuji; Saito, Hideaki; Ohta, Shozo
  • Nature Biotechnology, Vol. 14, Issue 6, p. 745-750
  • DOI: 10.1038/nbt0696-745

Expression in Escherichia coli of a chemically synthesized gene for the hormone somatostatin
journal, December 1977


Synthesis and Use of Synthetic Oligonucleotides
journal, June 1984


Regeneration of transgenic, microspore-derived, fertile barley
journal, October 1994

  • Jähne, A.; Becker, D.; Brettschneider, R.
  • Theoretical and Applied Genetics, Vol. 89, Issue 4
  • DOI: 10.1007/BF00225390

Novel seed lipid phenotypes in combinations of mutants altered in fatty acid biosynthesis inArabidopsis
journal, January 1991

  • James, D. W.; Dooner, H. K.
  • Theoretical and Applied Genetics, Vol. 82, Issue 4
  • DOI: 10.1007/BF00588591

Rapid and efficient purification of native histidine-tagged protein expressed by recombinant vaccinia virus.
journal, October 1991

  • Janknecht, R.; de Martynoff, G.; Lou, J.
  • Proceedings of the National Academy of Sciences, Vol. 88, Issue 20
  • DOI: 10.1073/pnas.88.20.8972

Putative polyadenylation signals in nuclear genes of higher plants: a compilation and analysis
journal, January 1987


An inspection of the domain between putative TATA box and translation start site in 79 plant genes
journal, January 1987


A short amino acid sequence able to specify nuclear location
journal, December 1984


Factors Influencing Gene Delivery into Zea Mays Cells by High–Velocity Microprojectiles
journal, May 1988

  • Klein, T. M.; Gradziel, T.; Fromm, M. E.
  • Nature Biotechnology, Vol. 6, Issue 5
  • DOI: 10.1038/nbt0588-559

Transformation of the developing barley endosperm by particle bombardment
journal, October 1991


Continuous cultures of fused cells secreting antibody of predefined specificity
journal, August 1975

  • Köhler, G.; Milstein, C.
  • Nature, Vol. 256, Issue 5517, p. 495-497
  • DOI: 10.1038/256495a0

The production of monoclonal antibodies from human lymphocytes
journal, March 1983


Field Performance of Elite Transgenic Maize Plants Expressing an Insecticidal Protein Derived from Bacillus thuringiensis
journal, February 1993

  • Koziel, Michael G.; Beland, Gary L.; Bowman, Cindy
  • Nature Biotechnology, Vol. 11, Issue 2
  • DOI: 10.1038/nbt0293-194

Transgenic Maize for the Control of European Corn Borer and Other Maize Insect Pests
journal, May 1996

  • Koziel, Michael G.; Carozzi, Nadine B.; Desai, Nalini
  • Annals of the New York Academy of Sciences, Vol. 792, Issue 1 Engineering P
  • DOI: 10.1111/j.1749-6632.1996.tb32502.x

In vitro transformation of plant protoplasts with Ti-plasmid DNA
journal, March 1982

  • Krens, F. A.; Molendijk, L.; Wullems, G. J.
  • Nature, Vol. 296, Issue 5852
  • DOI: 10.1038/296072a0

Predicting transmembrane protein topology with a hidden markov model: application to complete genomes11Edited by F. Cohen
journal, January 2001

  • Krogh, Anders; Larsson, Björn; von Heijne, Gunnar
  • Journal of Molecular Biology, Vol. 305, Issue 3
  • DOI: 10.1006/jmbi.2000.4315

A Mutant of Arabidopsis Deficient in Desaturation of Palmitic Acid in Leaf Lipids
journal, July 1989

  • Kunst, Ljerka; Browse, John; Somerville, Chris
  • Plant Physiology, Vol. 90, Issue 3
  • DOI: 10.1104/pp.90.3.943

Targeting of T7 RNA polymerase to tobacco nuclei mediated by an SV40 nuclear location signal
journal, August 1991

  • Lassner, Michael W.; Jones, Aubrey; Daubert, Steve
  • Plant Molecular Biology, Vol. 17, Issue 2
  • DOI: 10.1007/BF00039497

Intron enhancement of gene expression and the splicing efficiency of introns in maize cells
journal, January 1991

  • Luehrsen, KennethR.; Walbot, Virginia
  • MGG Molecular & General Genetics, Vol. 225, Issue 1
  • DOI: 10.1007/BF00282645

A desaturase-like protein from white spruce is a Δ9 desaturase
journal, August 2002

  • Marillia, Elizabeth-France; Giblin, E. Michael; Covello, Patrick S.
  • FEBS Letters, Vol. 526, Issue 1-3, p. 49-52
  • DOI: 10.1016/S0014-5793(02)03112-5

The synthesis of oligodeoxyprimidines on a polymer support
journal, January 1980


Stable Transformation of Soybean (Glycine Max) by Particle Acceleration
journal, August 1988

  • McCabe, Dennis E.; Swain, William F.; Martinell, Brian J.
  • Nature Biotechnology, Vol. 6, Issue 8
  • DOI: 10.1038/nbt0888-923

Toward a Functional Catalog of the Plant Genome. A Survey of Genes for Lipid Biosynthesis
journal, February 2000

  • Mekhedov, Sergei; de Ilárduya, Oskar Martı́nez; Ohlrogge, John
  • Plant Physiology, Vol. 122, Issue 2
  • DOI: 10.1104/pp.122.2.389

Ribozymes Targeted to Stearoyl–ACP Δ9 Desaturase mRNA Produce Heritable Increases of Stearic Acid in Transgenic Maize Leaves
journal, October 1998


Tales of poly(A): a review
journal, July 1990


Codon usage in plant genes
journal, January 1989

  • Murray, Elizabeth E.; Lotzer, Jeff; Eberle, Mary
  • Nucleic Acids Research, Vol. 17, Issue 2
  • DOI: 10.1093/nar/17.2.477

DNA synthesis
journal, January 1983


Identification of DNA sequences required for activity of the cauliflower mosaic virus 35S promoter
journal, February 1985

  • Odell, Joan T.; Nagy, Ferenc; Chua, Nam-Hai
  • Nature, Vol. 313, Issue 6005, p. 810-812
  • DOI: 10.1038/313810a0

A strategy for a convergent synthesis of N-linked glycopeptides on a solid support
journal, July 1995


Vectors for selective expression of cloned DNAs by T7 RNA polymerase
journal, January 1987


Linoleate and α-linolenate synthesis by isolated spinach (Spinacia oleracea) chloroplasts
journal, December 1979

  • Roughan, P. G.; Mudd, J. B.; McManus, T. T.
  • Biochemical Journal, Vol. 184, Issue 3
  • DOI: 10.1042/bj1840571

Metabolism of exogenous long-chain fatty acids by spinach leaves
journal, December 1987

  • Roughan, P. Grattan; Thompson, Guy A.; Cho, Sung Ho
  • Archives of Biochemistry and Biophysics, Vol. 259, Issue 2, p. 481-496
  • DOI: 10.1016/0003-9861(87)90515-7

A dissection of the cauliflower mosaic virus polyadenylation signal.
journal, January 1991

  • Sanfacon, H.; Brodmann, P.; Hohn, T.
  • Genes & Development, Vol. 5, Issue 1
  • DOI: 10.1101/gad.5.1.141

Delivery of Substances into Cells and Tissues Using a Particle Bombardment Process
journal, January 1987

  • Sanford, John C.; Klein, Theodore M.; Wolf, Edward D.
  • Particulate Science and Technology, Vol. 5, Issue 1
  • DOI: 10.1080/02726358708904533

Fertile transgenic rice plants regenerated from transformed protoplasts
journal, March 1989

  • Shimamoto, Ko; Terada, Rie; Izawa, Takeshi
  • Nature, Vol. 338, Issue 6212, p. 274-276
  • DOI: 10.1038/338274a0

Expression of a truncated tomato polygalacturonase gene inhibits expression of the endogenous gene in transgenic plants
journal, December 1990

  • Smith, C. J. S.; Watson, C. F.; Bird, C. R.
  • MGG Molecular & General Genetics, Vol. 224, Issue 3
  • DOI: 10.1007/BF00262443

Bialaphos selection of stable transformants from maize cell culture
journal, May 1990

  • Spencer, T. M.; Gordon-Kamm, W. J.; Daines, R. J.
  • Theoretical and Applied Genetics, Vol. 79, Issue 5
  • DOI: 10.1007/BF00226875

Desaturases fused to their electron donor
journal, March 2001


Transgenic Plants as Tools to Study the Molecular Organization of Plant Genes
journal, September 1987


Long regions of homologous DNA are incorporated into the tobacco plastid genome by transformation.
journal, January 1992


Rapid evolution of a protein in vitro by DNA shuffling
journal, August 1994


hpRNA-Mediated Targeting of the Arabidopsis FAD2 Gene Gives Highly Efficient and Stable Silencing
journal, July 2002

  • Stoutjesdijk, Peter A.; Singh, Surinder P.; Liu, Qing
  • Plant Physiology, Vol. 129, Issue 4
  • DOI: 10.1104/pp.006353

Isoforms of acyl carrier protein involved in seed‐specific fatty acid synthesis
journal, March 1999


Isoforms of acyl carrier protein involved in seed‐specific fatty acid synthesis
journal, March 1999


Stable transformation of plastids in higher plants.
journal, November 1990

  • Svab, Z.; Hajdukiewicz, P.; Maliga, P.
  • Proceedings of the National Academy of Sciences, Vol. 87, Issue 21
  • DOI: 10.1073/pnas.87.21.8526

High-frequency plastid transformation in tobacco by selection for a chimeric aadA gene.
journal, February 1993

  • Svab, Z.; Maliga, P.
  • Proceedings of the National Academy of Sciences, Vol. 90, Issue 3
  • DOI: 10.1073/pnas.90.3.913

Why are proteins marginally stable?
journal, December 2001

  • Taverna, Darin M.; Goldstein, Richard A.
  • Proteins: Structure, Function, and Genetics, Vol. 46, Issue 1
  • DOI: 10.1002/prot.10016

Isolation and Characterization of a Gene Affecting Fatty Acid Elongation in Saccharomyces cerevisiae
journal, August 1996


Use of paromomycin as a selective agent for oat transformation
journal, July 1995

  • Torbert, Kimberly A.; Rines, Howard W.; Somers, David A.
  • Plant Cell Reports, Vol. 14, Issue 10
  • DOI: 10.1007/BF00232728

The HMMTOP transmembrane topology prediction server
journal, September 2001


Genetically Transformed Cotton (Gossypium Hirsutum L.) Plants
journal, March 1987

  • Umbeck, Paul; Johnson, Gail; Barton, Ken
  • Bio/Technology, Vol. 5, Issue 3
  • DOI: 10.1038/nbt0387-263

Flavonoid genes in petunia: addition of a limited number of gene copies may lead to a suppression of gene expression.
journal, April 1990

  • van der Krol, A. R.; Mur, L. A.; Beld, M.
  • The Plant Cell, Vol. 2, Issue 4
  • DOI: 10.1105/tpc.2.4.291

Rapid Production of Transgenic Wheat Plants by Direct Bombardment of Cultured Immature Embryos
journal, December 1993

  • Vasil, Vimla; Srivastava, Vibha; Castillo, Ana M.
  • Nature Biotechnology, Vol. 11, Issue 12
  • DOI: 10.1038/nbt1293-1553

The pUC plasmids, an M13mp7-derived system for insertion mutagenesis and sequencing with synthetic universal primers
journal, October 1982


Photoinhibition in Mutants of Arabidopsis Deficient in Thylakoid Unsaturation
journal, May 2002

  • Vijayan, Perumal; Browse, John
  • Plant Physiology, Vol. 129, Issue 2
  • DOI: 10.1104/pp.004341

The role of enhancers in the regulation of cell-type-specific transcriptional control
journal, July 1986

  • Voss, Stephan D.; Schlokat, Uwe; Gruss, Peter
  • Trends in Biochemical Sciences, Vol. 11, Issue 7, p. 287-289
  • DOI: 10.1016/0968-0004(86)90031-9

Generation of Large Numbers of Independently Transformed Fertile Barley Plants
journal, January 1994


Rapid Production of Multiple Independent Lines of Fertile Transgenic Wheat (Triticum aestivum)
journal, August 1993

  • Weeks, J. T.; Anderson, O. D.; Blechl, A. E.
  • Plant Physiology, Vol. 102, Issue 4
  • DOI: 10.1104/pp.102.4.1077

Foreign Genes in Plants: Transfer, Structure, Expression, and Applications
journal, December 1988


Construct design for efficient, effective and high-throughput gene silencing in plants: Construct design for gene silencing
journal, September 2001


A cassette containing the bar gene of Streptomyces hygroscopicus : a selectable marker for plant transformation
journal, January 1990

  • White, Janet; Chang, Sheng-Yung P.; Bibb, Maureen J.
  • Nucleic Acids Research, Vol. 18, Issue 4
  • DOI: 10.1093/nar/18.4.1062

The structure of an antigenic determinant in a protein
journal, July 1984


Determination of double-bond positions in methylene-interrupted dienoic fatty acids by GC-MS as their dimethyl disulfide adducts
journal, November 1991

  • Yamamoto, Kohei; Shibahara, Akira; Nakayama, Takao
  • Chemistry and Physics of Lipids, Vol. 60, Issue 1, p. 39-50
  • DOI: 10.1016/0009-3084(91)90013-2

Expression of the Arabidopsis ADS1 gene in Brassica juncea results in a decreased level of total saturated fatty acids: ��9 desaturase over-expression in B. juncea
journal, April 2003


Optimization of DNA shuffling for high fidelity recombination
journal, March 1997