Oxygen-resistant hydrogenases and methods for designing and making same

King, Paul; Ghirardi, Maria Lucia; Seibert, Michael

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Title: Oxygen-resistant hydrogenases and methods for designing and making same

Abstract

The invention provides oxygen-resistant iron-hydrogenases ([Fe]-hydrogenases) for use in the production of H.sub.2. Methods used in the design and engineering of the oxygen-resistant [Fe]-hydrogenases are disclosed, as are the methods of transforming and culturing appropriate host cells with the oxygen-resistant [Fe]-hydrogenases. Finally, the invention provides methods for utilizing the transformed, oxygen insensitive, host cells in the bulk production of H.sub.2 in a light catalyzed reaction having water as the reactant.

Inventors:: King, Paul; Ghirardi, Maria Lucia; Seibert, Michael

Issue Date:: Tue Mar 04 00:00:00 EST 2014

Research Org.:: National Renewable Energy Laboratory (NREL), Golden, CO (United States)

Sponsoring Org.:: USDOE

OSTI Identifier:: 1127083

Patent Number(s):: 8663958

Application Number:: 12/360,756

Assignee:: Alliance for Sustainable Energy, LLC (Golden, CO)

Patent Classifications (CPCs):: C - CHEMISTRY C12 - BIOCHEMISTRY C12N - MICROORGANISMS OR ENZYMES

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C - CHEMISTRY C12 - BIOCHEMISTRY C12N - MICROORGANISMS OR ENZYMES
C12N9/001 - {acting on the CH-CH group of donors
C12N9/0067 - {acting on hydrogen as donor

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DOE Contract Number:: AC36-08GO28308

Resource Type:: Patent

Country of Publication:: United States

Language:: English

Subject:: 59 BASIC BIOLOGICAL SCIENCES

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                    King, Paul, Ghirardi, Maria Lucia, and Seibert, Michael. Oxygen-resistant hydrogenases and methods for designing and making same.  United States: N. p., 2014. 
        Web.

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                    King, Paul, Ghirardi, Maria Lucia, & Seibert, Michael. Oxygen-resistant hydrogenases and methods for designing and making same.  United States.

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                    King, Paul, Ghirardi, Maria Lucia, and Seibert, Michael. Tue .  
        "Oxygen-resistant hydrogenases and methods for designing and making same".  United States.  https://www.osti.gov/servlets/purl/1127083.

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@article{osti_1127083,

  title        = {Oxygen-resistant hydrogenases and methods for designing and making same},

  author       = {King, Paul and Ghirardi, Maria Lucia and Seibert, Michael},

  abstractNote = {The invention provides oxygen-resistant iron-hydrogenases ([Fe]-hydrogenases) for use in the production of H.sub.2. Methods used in the design and engineering of the oxygen-resistant [Fe]-hydrogenases are disclosed, as are the methods of transforming and culturing appropriate host cells with the oxygen-resistant [Fe]-hydrogenases. Finally, the invention provides methods for utilizing the transformed, oxygen insensitive, host cells in the bulk production of H.sub.2 in a light catalyzed reaction having water as the reactant.},

  doi          = {},

  journal      = {},
number       = ,

  volume       = ,

  place        = {United States},

  year         = {Tue Mar 04 00:00:00 EST 2014},

  month        = {Tue Mar 04 00:00:00 EST 2014}

}

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