Global to push GA events into
skip to main content

Title: Compositions of orthogonal lysyl-tRNA and aminoacyl-tRNA synthetase pairs and uses thereof

Compositions and methods of producing components of protein biosynthetic machinery that include orthogonal lysyl-tRNAs, orthogonal lysyl-aminoacyl-tRNA synthetases, and orthogonal pairs of lysyl-tRNAs/synthetases, which incorporate homoglutamines into proteins are provided in response to a four base codon. Methods for identifying these orthogonal pairs are also provided along with methods of producing proteins with homoglutamines using these orthogonal pairs.
Inventors:
; ; ;
Issue Date:
OSTI Identifier:
1126706
Assignee:
The Scripps Research Institute (La Jolla, CA) DOESC
Patent Number(s):
8,669,073
Application Number:
13/246,490
Contract Number:
ER45812
Research Org:
The Scripps Research Institute, La Jolla, CA, USA
Sponsoring Org:
USDOE
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES

Other works cited in this record:

Glycoprotein synthesis
patent, August 2005

In vivo incorporation of unnatural amino acids
patent, May 2006

Methods and compositions for the production of orthogonal tRNA-aminoacyl tRNA synthetase pairs
patent, August 2006

Glycoprotein synthesis
patent, October 2006

Glycoprotein synthesis
patent, February 2007

Glycoprotein synthesis
patent, April 2007

Glycoprotein synthesis
patent, May 2007

Glycoprotein synthesis
patent, July 2007

Glycoprotein synthesis
patent, August 2007

Methods and composition for the production of orthogonal tRNA-aminoacyltRNA synthetase pairs
patent, April 2008

In vivo incorporation of unnatural amino acids
patent, May 2008

In vivo site-specific incorporation of N-acetyl-galactosamine amino acids in eubacteria
patent, May 2008

Site specific incorporation of heavy atom-containing unnatural amino acids into proteins for structure determination
patent, July 2008

Site specific incorporation of keto amino acids into proteins
patent, October 2008

Site-specific incorporation of redox active amino acids into proteins
patent, February 2009

Site specific incorporation of keto amino acids into proteins
patent, April 2009

Compositions of orthogonal glutamyl-tRNA and aminoacyl-tRNA synthetase pairs and uses thereof
patent, May 2009

Glycoprotein synthesis
patent, July 2009

Compositions of orthogonal lysyl-tRNA and aminoacyl-tRNA synthetase pairs and uses thereof
patent, August 2009

Expanding the eukaryotic genetic code
patent, October 2009

Compositions of orthogonal lysyl-tRNA and aminoacyl-tRNA synthetase pairs and uses thereof
patent, December 2009

In vivo incorporation of unnatural amino acids
patent, December 2009

Protein arrays
patent, January 2010

Unnatural reactive amino acid genetic code additions
patent-application, December 2004

Expanding the eukaryotic genetic code
patent-application, January 2005

Selective incorporation of 5-hydroxytryptophan into proteins in mammalian cells
patent-application, June 2005

Methods and composition for the production of orthogonal tRNA-aminoacyltRNA synthetase pairs
patent-application, September 2005

In vivo incorporation of unnatural amino acids
patent-application, November 2005

Methods and composition for the production of orthogonal tRNA-aminoacyltRNA synthetase pairs
patent-application, March 2006

In vivo incorporation of alkynyl amino acids into proteins in eubacteria
patent-application, April 2006

Site-specific labeling of proteins for NMR studies
patent-application, May 2006

In vivo site-specific incorporation of N-acetyl-galactosamine amino acids in eubacteria
patent-application, May 2006

Adding photoregulated amino acids to the genetic code
patent-application, June 2006

Compositions of orthogonal leucyl-trna and aminoacyl-trna synthetase pairs and uses thereof
patent-application, July 2006

Unnatural reactive amino acid genetic code additions
patent-application, November 2006

Site-specific incorporation of redox active amino acids into proteins
patent-application, January 2007

Selective incorporation of 5-hydroxytryptophan into proteins in mammalian cells
patent-application, May 2007

Expanding the eukaryotic genetic code
patent-application, July 2007

Glycoprotein synthesis
patent-application, July 2007

Selective posttranslational modification of phage-displayed polypeptides
patent-application, August 2007

Genetically programmed expression of proteins containing the unnatural amino acid phenylselenocysteine
patent-application, October 2007

Systems for the expression of orthogonal translation components in eubacterial host cells
patent-application, December 2007

Site specific incorporation of keto amino acids into proteins
patent-application, September 2008

Exploring the Limits of Codon and Anticodon Size
journal, February 2002
  • Anderson, J. Christopher; Magliery, Thomas J.; Schultz, Peter G.
  • Chemistry & Biology, Vol. 9, Issue 2, p. 237-244
  • DOI: 10.1016/S1074-5521(02)00094-7

An expanded genetic code with a functional quadruplet codon
journal, May 2004
  • Anderson, J. C.; Wu, N.; Santoro, S. W.
  • Proceedings of the National Academy of Sciences, Vol. 101, Issue 20, p. 7566-7571
  • DOI: 10.1073/pnas.0401517101

Four-base codons ACCA, ACCU and ACCC are recognized by frameshift suppressor sufJ
journal, August 1981

In Vivo Photocrosslinking with Unnatural Amino Acid Mutagenesis
journal, November 2002

Addition of a photocrosslinking amino acid to the genetic code of Escherichia coli
journal, August 2002
  • Chin, J. W.; Martin, A. B.; King, D. S.
  • Proceedings of the National Academy of Sciences, Vol. 99, Issue 17, p. 11020-11024
  • DOI: 10.1073/pnas.172226299

An Expanded Eukaryotic Genetic Code
journal, August 2003
  • Chin, Jason W.; Cropp, T. Ashton; Anderson, J. Christopher
  • Science, Vol. 301, Issue 5635, p. 964-967
  • DOI: 10.1126/science.1084772

Expansion of the genetic code: Site-directed p-fluoro-phenylalanine incorporation in Escherichia coli
journal, February 1998

Expanding the genetic code: selection of efficient suppressors of four-base codons and identification of “shifty” four-base codons with a library approach in Escherichia coli
journal, March 2001
  • Magliery, Thomas J.; Anderson, J. Christopher; Schultz, Peter G.
  • Journal of Molecular Biology, Vol. 307, Issue 3, p. 755-769
  • DOI: 10.1006/jmbi.2001.4518

Generation of a Bacterium with a 21 Amino Acid Genetic Code
journal, January 2003
  • Mehl, Ryan A.; Anderson, J. Christopher; Santoro, Stephen W.
  • Journal of the American Chemical Society, Vol. 125, Issue 4, p. 935-939
  • DOI: 10.1021/ja0284153

A New Functional Suppressor tRNA/Aminoacyl−tRNA Synthetase Pair for the in Vivo Incorporation of Unnatural Amino Acids into Proteins
journal, May 2000
  • Wang, Lei; Magliery, Thomas J.; Liu, David R.
  • Journal of the American Chemical Society, Vol. 122, Issue 20, p. 5010-5011
  • DOI: 10.1021/ja000595y

Expanding the Genetic Code
journal, January 2005
  • Wang, Lei; Schultz, Peter G.
  • Angewandte Chemie International Edition, Vol. 44, Issue 1, p. 34-66
  • DOI: 10.1002/anie.200460627

Addition of the keto functional group to the genetic code of Escherichia coli
journal, December 2002
  • Wang, L.; Zhang, Z.; Brock, A.
  • Proceedings of the National Academy of Sciences, Vol. 100, Issue 1, p. 56-61
  • DOI: 10.1073/pnas.0234824100

Actions of the anticodon arm in translation on the phenotypes of RNA mutants
journal, November 1986

Similar records in DOepatents and OSTI.GOV collections: