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Title: Compositions of orthogonal lysyl-tRNA and aminoacyl-tRNA synthetase pairs and uses thereof

Abstract

Compositions and methods of producing components of protein biosynthetic machinery that include orthogonal lysyl-tRNAs, orthogonal lysyl-aminoacyl-tRNA synthetases, and orthogonal pairs of lysyl-tRNAs/synthetases, which incorporate homoglutamines into proteins are provided in response to a four base codon. Methods for identifying these orthogonal pairs are also provided along with methods of producing proteins with homoglutamines using these orthogonal pairs.

Inventors:
; ; ;
Issue Date:
Research Org.:
The Scripps Research Institute, La Jolla, CA, USA
Sponsoring Org.:
USDOE
OSTI Identifier:
1126706
Patent Number(s):
8669073
Application Number:
13/246,490
Assignee:
The Scripps Research Institute (La Jolla, CA)
Patent Classifications (CPCs):
C - CHEMISTRY C12 - BIOCHEMISTRY C12N - MICROORGANISMS OR ENZYMES
C - CHEMISTRY C12 - BIOCHEMISTRY C12P - FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE {
DOE Contract Number:  
ER45812
Resource Type:
Patent
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES

Citation Formats

Anderson, J. Christopher, Wu, Ning, Santoro, Stephen, and Schultz, Peter G. Compositions of orthogonal lysyl-tRNA and aminoacyl-tRNA synthetase pairs and uses thereof. United States: N. p., 2014. Web.
Anderson, J. Christopher, Wu, Ning, Santoro, Stephen, & Schultz, Peter G. Compositions of orthogonal lysyl-tRNA and aminoacyl-tRNA synthetase pairs and uses thereof. United States.
Anderson, J. Christopher, Wu, Ning, Santoro, Stephen, and Schultz, Peter G. Tue . "Compositions of orthogonal lysyl-tRNA and aminoacyl-tRNA synthetase pairs and uses thereof". United States. https://www.osti.gov/servlets/purl/1126706.
@article{osti_1126706,
title = {Compositions of orthogonal lysyl-tRNA and aminoacyl-tRNA synthetase pairs and uses thereof},
author = {Anderson, J. Christopher and Wu, Ning and Santoro, Stephen and Schultz, Peter G},
abstractNote = {Compositions and methods of producing components of protein biosynthetic machinery that include orthogonal lysyl-tRNAs, orthogonal lysyl-aminoacyl-tRNA synthetases, and orthogonal pairs of lysyl-tRNAs/synthetases, which incorporate homoglutamines into proteins are provided in response to a four base codon. Methods for identifying these orthogonal pairs are also provided along with methods of producing proteins with homoglutamines using these orthogonal pairs.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {2014},
month = {3}
}

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Works referenced in this record:

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