skip to main content
DOE Patents title logo U.S. Department of Energy
Office of Scientific and Technical Information

Title: Compositions of orthogonal lysyl-tRNA and aminoacyl-tRNA synthetase pairs and uses thereof

Abstract

Compositions and methods of producing components of protein biosynthetic machinery that include orthogonal lysyl-tRNAs, orthogonal lysyl-aminoacyl-tRNA synthetases, and orthogonal pairs of lysyl-tRNAs/synthetases, which incorporate homoglutamines into proteins are provided in response to a four base codon. Methods for identifying these orthogonal pairs are also provided along with methods of producing proteins with homoglutamines using these orthogonal pairs.

Inventors:
; ; ;
Issue Date:
Research Org.:
The Scripps Research Institute, La Jolla, CA, USA
Sponsoring Org.:
USDOE
OSTI Identifier:
1126706
Patent Number(s):
8,669,073
Application Number:
13/246,490
Assignee:
The Scripps Research Institute (La Jolla, CA)
DOE Contract Number:  
ER45812
Resource Type:
Patent
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES

Citation Formats

Anderson, J. Christopher, Wu, Ning, Santoro, Stephen, and Schultz, Peter G. Compositions of orthogonal lysyl-tRNA and aminoacyl-tRNA synthetase pairs and uses thereof. United States: N. p., 2014. Web.
Anderson, J. Christopher, Wu, Ning, Santoro, Stephen, & Schultz, Peter G. Compositions of orthogonal lysyl-tRNA and aminoacyl-tRNA synthetase pairs and uses thereof. United States.
Anderson, J. Christopher, Wu, Ning, Santoro, Stephen, and Schultz, Peter G. Tue . "Compositions of orthogonal lysyl-tRNA and aminoacyl-tRNA synthetase pairs and uses thereof". United States. https://www.osti.gov/servlets/purl/1126706.
@article{osti_1126706,
title = {Compositions of orthogonal lysyl-tRNA and aminoacyl-tRNA synthetase pairs and uses thereof},
author = {Anderson, J. Christopher and Wu, Ning and Santoro, Stephen and Schultz, Peter G},
abstractNote = {Compositions and methods of producing components of protein biosynthetic machinery that include orthogonal lysyl-tRNAs, orthogonal lysyl-aminoacyl-tRNA synthetases, and orthogonal pairs of lysyl-tRNAs/synthetases, which incorporate homoglutamines into proteins are provided in response to a four base codon. Methods for identifying these orthogonal pairs are also provided along with methods of producing proteins with homoglutamines using these orthogonal pairs.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {2014},
month = {3}
}

Patent:

Save / Share:

Works referenced in this record:

Functional Annotation of Class I Lysyl-tRNA Synthetase Phylogeny Indicates a Limited Role for Gene Transfer
journal, August 2002


Adaptation of an Orthogonal Archaeal Leucyl-tRNA and Synthetase Pair for Four-base, Amber, and Opal Suppression
journal, August 2003

  • Anderson, J. Christopher; Schultz, Peter G.
  • Biochemistry, Vol. 42, Issue 32
  • DOI: 10.1021/bi034550w

Exploring the Limits of Codon and Anticodon Size
journal, February 2002

  • Anderson, J. Christopher; Magliery, Thomas J.; Schultz, Peter G.
  • Chemistry & Biology, Vol. 9, Issue 2, p. 237-244
  • DOI: 10.1016/S1074-5521(02)00094-7

An expanded genetic code with a functional quadruplet codon
journal, May 2004

  • Anderson, J. C.; Wu, N.; Santoro, S. W.
  • Proceedings of the National Academy of Sciences, Vol. 101, Issue 20, p. 7566-7571
  • DOI: 10.1073/pnas.0401517101

Four-base codons ACCA, ACCU and ACCC are recognized by frameshift suppressor sufJ
journal, August 1981


Properties of the lysyl-tRNA synthetase gene and product from the extreme thermophile Thermus thermophilus.
journal, May 1994


In Vivo Photocrosslinking with Unnatural Amino Acid Mutagenesis
journal, November 2002


Addition of a photocrosslinking amino acid to the genetic code of Escherichia coli
journal, August 2002

  • Chin, J. W.; Martin, A. B.; King, D. S.
  • Proceedings of the National Academy of Sciences, Vol. 99, Issue 17, p. 11020-11024
  • DOI: 10.1073/pnas.172226299

An Expanded Eukaryotic Genetic Code
journal, August 2003

  • Chin, Jason W.; Cropp, T. Ashton; Anderson, J. Christopher
  • Science, Vol. 301, Issue 5635, p. 964-967
  • DOI: 10.1126/science.1084772

Reading frame selection and transfer RNA anticodon loop stacking
journal, December 1987


Expansion of the genetic code: Site-directed p-fluoro-phenylalanine incorporation in Escherichia coli
journal, February 1998


Incorporation of non-natural amino acids into proteins
journal, December 2002


Incorporation of Nonnatural Amino Acids into Streptavidin through In Vitro Frame-Shift Suppression
journal, January 1996

  • Hohsaka, Takahiro; Ashizuka, Yuki; Murakami, Hiroshi
  • Journal of the American Chemical Society, Vol. 118, Issue 40
  • DOI: 10.1021/ja9614225

Five-base codons for incorporation of nonnatural amino acids into proteins
journal, September 2001

  • Hohsaka, Takahiro; Ashizuka, Yuki; Murakami, Hiroshi
  • Nucleic Acids Research, Vol. 29, Issue 17, p. 3646-3651
  • DOI: 10.1093/nar/29.17.3646

Incorporation of two nonnatural amino acids into proteins through extension of the genetic code
journal, November 1999

  • Hohsaka, T.; Ashizuka, Y.; Sisido, M.
  • Nucleic Acids Symposium Series, Vol. 42, Issue 1
  • DOI: 10.1093/nass/42.1.79

Substrate recognition by class I lysyl-tRNA synthetases: A molecular basis for gene displacement
journal, January 1999

  • Ibba, M.; Losey, H. C.; Kawarabayasi, Y.
  • Proceedings of the National Academy of Sciences, Vol. 96, Issue 2
  • DOI: 10.1073/pnas.96.2.418

Structural basis for orthogonal tRNA specificities of tyrosyl-tRNA synthetases for genetic code expansion
journal, May 2003

  • Kobayashi, Takatsugu; Nureki, Osamu; Ishitani, Ryuichiro
  • Nature Structural & Molecular Biology, Vol. 10, Issue 6
  • DOI: 10.1038/nsb934

Evolutionary relationship between Halobacterium cutirubrum and eukaryotes determined by use of aminoacyl-tRNA synthetases as phylogenetic probes
journal, March 1980

  • Kwok, Yau; Wong, J. Tze-Fei
  • Canadian Journal of Biochemistry, Vol. 58, Issue 3
  • DOI: 10.1139/o80-029

Expanding the genetic code: selection of efficient suppressors of four-base codons and identification of “shifty” four-base codons with a library approach in Escherichia coli
journal, March 2001

  • Magliery, Thomas J.; Anderson, J. Christopher; Schultz, Peter G.
  • Journal of Molecular Biology, Vol. 307, Issue 3, p. 755-769
  • DOI: 10.1006/jmbi.2001.4518

Generation of a Bacterium with a 21 Amino Acid Genetic Code
journal, January 2003

  • Mehl, Ryan A.; Anderson, J. Christopher; Santoro, Stephen W.
  • Journal of the American Chemical Society, Vol. 125, Issue 4, p. 935-939
  • DOI: 10.1021/ja0284153

Insertions in the anticodon loop of tRNA1Gln(sufG) and tRNALys promote quadruplet decoding of CAAA
journal, May 2002


An efficient system for the evolution of aminoacyl-tRNA synthetase specificity
journal, September 2002

  • Santoro, Stephen W.; Wang, Lei; Herberich, Brad
  • Nature Biotechnology, Vol. 20, Issue 10
  • DOI: 10.1038/nbt742

Functional convergence of two lysyl-tRNA synthetases with unrelated topologies
journal, March 2002

  • Terada, Takaho; Nureki, Osamu; Ishitani, Ryuichiro
  • Nature Structural Biology, Vol. 9, Issue 4
  • DOI: 10.1038/nsb777

A New Functional Suppressor tRNA/Aminoacyl−tRNA Synthetase Pair for the in Vivo Incorporation of Unnatural Amino Acids into Proteins
journal, May 2000

  • Wang, Lei; Magliery, Thomas J.; Liu, David R.
  • Journal of the American Chemical Society, Vol. 122, Issue 20, p. 5010-5011
  • DOI: 10.1021/ja000595y

Expanding the Genetic Code
journal, January 2005

  • Wang, Lei; Schultz, Peter G.
  • Angewandte Chemie International Edition, Vol. 44, Issue 1, p. 34-66
  • DOI: 10.1002/anie.200460627

Addition of the keto functional group to the genetic code of Escherichia coli
journal, December 2002

  • Wang, L.; Zhang, Z.; Brock, A.
  • Proceedings of the National Academy of Sciences, Vol. 100, Issue 1, p. 56-61
  • DOI: 10.1073/pnas.0234824100

Actions of the anticodon arm in translation on the phenotypes of RNA mutants
journal, November 1986