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Title: Selective incorporation of 5-hydroxytryptophan into proteins in mammalian cells

This invention provides methods and compositions for incorporation of an unnatural amino acid into a peptide using an orthogonal aminoacyl tRNA synthetase/tRNA pair. In particular, an orthogonal pair is provided to incorporate 5-hydroxy-L-tryptophan in a position encoded by an opal mutation.
Inventors:
; ;
Issue Date:
OSTI Identifier:
1126420
Assignee:
The Scripps Research Institute (La Jolla, CA) OAK
Patent Number(s):
8,658,395
Application Number:
13/546,582
Contract Number:
FG03-00ER45812
Research Org:
The Scripps Research Institute, La Jolla, CA, USA
Sponsoring Org:
USDOE
Country of Publication:
United States
Language:
English
Subject:
60 APPLIED LIFE SCIENCES

Other works cited in this record:

In vivo incorporation of unnatural amino acids
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In vivo incorporation of unnatural amino acids
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Methods and compositions for the production of orthogonal tRNA-aminoacyltRNA synthetase pairs
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Import of amber and ochre suppressor tRNAs into mammalian cells: A general approach to site-specific insertion of amino acid analogues into proteins
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A New Orthogonal Suppressor tRNA/Aminoacyl-tRNA Synthetase Pair for Evolving an Organism with an Expanded Genetic Code
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Site-specific incorporation of an unnatural amino acid into proteins in mammalian cells
journal, November 2002
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From genes to protein structure and function: novel applications of computational approaches in the genomic era
journal, January 2000

A general approach for the generation of orthogonal tRNAs
journal, September 2001

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