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Title: Selective incorporation of 5-hydroxytryptophan into proteins in mammalian cells

Abstract

This invention provides methods and compositions for incorporation of an unnatural amino acid into a peptide using an orthogonal aminoacyl tRNA synthetase/tRNA pair. In particular, an orthogonal pair is provided to incorporate 5-hydroxy-L-tryptophan in a position encoded by an opal mutation.

Inventors:
; ;
Issue Date:
Research Org.:
The Scripps Research Institute, La Jolla, CA, USA
Sponsoring Org.:
USDOE
OSTI Identifier:
1126420
Patent Number(s):
8658395
Application Number:
13/546,582
Assignee:
The Scripps Research Institute (La Jolla, CA)
Patent Classifications (CPCs):
C - CHEMISTRY C12 - BIOCHEMISTRY C12N - MICROORGANISMS OR ENZYMES
C - CHEMISTRY C07 - ORGANIC CHEMISTRY C07K - PEPTIDES
DOE Contract Number:  
FG03-00ER45812
Resource Type:
Patent
Country of Publication:
United States
Language:
English
Subject:
60 APPLIED LIFE SCIENCES

Citation Formats

Zhang, Zhiwen, Alfonta, Lital, and Schultz, Peter G. Selective incorporation of 5-hydroxytryptophan into proteins in mammalian cells. United States: N. p., 2014. Web.
Zhang, Zhiwen, Alfonta, Lital, & Schultz, Peter G. Selective incorporation of 5-hydroxytryptophan into proteins in mammalian cells. United States.
Zhang, Zhiwen, Alfonta, Lital, and Schultz, Peter G. Tue . "Selective incorporation of 5-hydroxytryptophan into proteins in mammalian cells". United States. https://www.osti.gov/servlets/purl/1126420.
@article{osti_1126420,
title = {Selective incorporation of 5-hydroxytryptophan into proteins in mammalian cells},
author = {Zhang, Zhiwen and Alfonta, Lital and Schultz, Peter G},
abstractNote = {This invention provides methods and compositions for incorporation of an unnatural amino acid into a peptide using an orthogonal aminoacyl tRNA synthetase/tRNA pair. In particular, an orthogonal pair is provided to incorporate 5-hydroxy-L-tryptophan in a position encoded by an opal mutation.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {2014},
month = {2}
}

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Works referenced in this record:

Twenty-first aminoacyl-tRNA synthetase-suppressor tRNA pairs for possible use in site-specific incorporation of amino acid analogues into proteins in eukaryotes and in eubacteria
journal, January 2001


An Expanded Eukaryotic Genetic Code
journal, August 2003


Aminoacyl-tRNA synthetases
journal, December 1997


Adding Amino Acids with Novel Reactivity to the Genetic Code of Saccharomyces Cerevisiae
journal, October 2003

  • Deiters, Alexander; Cropp, T. Ashton; Mukherji, Mridul
  • Journal of the American Chemical Society, Vol. 125, Issue 39, p. 11782-11783
  • https://doi.org/10.1021/ja0370037

Protein tolerance to random amino acid change
journal, June 2004


The renaissance of aminoacyl-tRNA synthesis
journal, May 2001


A "Silent" Polymorphism in the MDR1 Gene Changes Substrate Specificity
journal, January 2007


Import of amber and ochre suppressor tRNAs into mammalian cells: A general approach to site-specific insertion of amino acid analogues into proteins
journal, November 2001


Progress toward the evolution of an organism with an expanded genetic code
journal, April 1999


Comparative Protein Structure Modeling of Genes and Genomes
journal, June 2000


A New Orthogonal Suppressor tRNA/Aminoacyl-tRNA Synthetase Pair for Evolving an Organism with an Expanded Genetic Code
journal, September 2000


Site-specific incorporation of an unnatural amino acid into proteins in mammalian cells
journal, November 2002


From genes to protein structure and function: novel applications of computational approaches in the genomic era
journal, January 2000


Identity elements and aminoacylation of plant tRNATrp
journal, November 1998


A general approach for the generation of orthogonal tRNAs
journal, September 2001