Selective incorporation of 5-hydroxytryptophan into proteins in mammalian cells

Zhang, Zhiwen; Alfonta, Lital; Schultz, Peter G

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Title: Selective incorporation of 5-hydroxytryptophan into proteins in mammalian cells

Abstract

This invention provides methods and compositions for incorporation of an unnatural amino acid into a peptide using an orthogonal aminoacyl tRNA synthetase/tRNA pair. In particular, an orthogonal pair is provided to incorporate 5-hydroxy-L-tryptophan in a position encoded by an opal mutation.

Inventors:: Zhang, Zhiwen; Alfonta, Lital; Schultz, Peter G

Issue Date:: 2014-02-25

Research Org.:: The Scripps Research Institute, La Jolla, CA, USA

Sponsoring Org.:: USDOE

OSTI Identifier:: 1126420

Patent Number(s):: 8658395

Application Number:: 13/546,582

Assignee:: The Scripps Research Institute (La Jolla, CA)

Patent Classifications (CPCs):: C - CHEMISTRY C07 - ORGANIC CHEMISTRY C07K - PEPTIDES

C - CHEMISTRY C12 - BIOCHEMISTRY C12N - MICROORGANISMS OR ENZYMES

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C - CHEMISTRY C07 - ORGANIC CHEMISTRY C07K - PEPTIDES
C07K14/47 - from mammals

C - CHEMISTRY C12 - BIOCHEMISTRY C12N - MICROORGANISMS OR ENZYMES
C12N15/67 - General methods for enhancing the expression
C12N9/93 - {Ligases

C - CHEMISTRY C12 - BIOCHEMISTRY C12P - FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE {
C12P21/02 - having a known sequence of two or more amino acids, e.g. glutathione

Show less

DOE Contract Number:: FG03-00ER45812

Resource Type:: Patent

Country of Publication:: United States

Language:: English

Subject:: 60 APPLIED LIFE SCIENCES

Citation Formats


                    Zhang, Zhiwen, Alfonta, Lital, and Schultz, Peter G. Selective incorporation of 5-hydroxytryptophan into proteins in mammalian cells.  United States: N. p., 2014. 
        Web.

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                    Zhang, Zhiwen, Alfonta, Lital, & Schultz, Peter G. Selective incorporation of 5-hydroxytryptophan into proteins in mammalian cells.  United States.

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                    Zhang, Zhiwen, Alfonta, Lital, and Schultz, Peter G. Tue .  
        "Selective incorporation of 5-hydroxytryptophan into proteins in mammalian cells".  United States.  https://www.osti.gov/servlets/purl/1126420.

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@article{osti_1126420,

  title        = {Selective incorporation of 5-hydroxytryptophan into proteins in mammalian cells},

  author       = {Zhang, Zhiwen and Alfonta, Lital and Schultz, Peter G},

  abstractNote = {This invention provides methods and compositions for incorporation of an unnatural amino acid into a peptide using an orthogonal aminoacyl tRNA synthetase/tRNA pair. In particular, an orthogonal pair is provided to incorporate 5-hydroxy-L-tryptophan in a position encoded by an opal mutation.},

  doi          = {},

  journal      = {},
number       = ,

  volume       = ,

  place        = {United States},

  year         = {2014},

  month        = {2}

}

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Works referenced in this record:

In vivo incorporation of unnatural amino acids
patent, May 2006

Schultz, Peter G.; Wang, Lei; Anderson, John Christopher
US Patent Document 7,045,337
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Protein tolerance to random amino acid change
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Characterization of aminoacyl-adenylates in B. tsubtilis tryptophanyl-tRNA synthetase, by the fluorescence of tryptophan analogs 5-hydroxytryptophan and 7-azatryptophan
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Selective incorporation of 5-hydroxytryptophan into proteins in mammalian cells

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Similar Records

Title: Selective incorporation of 5-hydroxytryptophan into proteins in mammalian cells

Abstract

Citation Formats

In vivo incorporation of unnatural amino acids patent, May 2006

In vivo incorporation of unnatural amino acids patent-application, May 2003

Methods and compositions for the production of orthogonal tRNA-aminoacyltRNA synthetase pairs patent-application, June 2003

Low brain uptake of L-[ 11 C]5-hydroxytryptophan in major depression: a positron emission tomography study on patients and healthy volunteers journal, June 1991

Twenty-first aminoacyl-tRNA synthetase-suppressor tRNA pairs for possible use in site-specific incorporation of amino acid analogues into proteins in eukaryotes and in eubacteria journal, January 2001

An Expanded Eukaryotic Genetic Code journal, August 2003

Cloning and nucleotide sequence of the structural gene coding for Bacillus subtilis tryptophanyl-tRNA synthetase journal, December 1988

Aminoacyl-tRNA synthetases journal, December 1997

Adding Amino Acids with Novel Reactivity to the Genetic Code of Saccharomyces Cerevisiae journal, October 2003

Protein tolerance to random amino acid change journal, June 2004

Characterization of aminoacyl-adenylates in B. tsubtilis tryptophanyl-tRNA synthetase, by the fluorescence of tryptophan analogs 5-hydroxytryptophan and 7-azatryptophan journal, December 1993

The renaissance of aminoacyl-tRNA synthesis journal, May 2001

A "Silent" Polymorphism in the MDR1 Gene Changes Substrate Specificity journal, January 2007

Import of amber and ochre suppressor tRNAs into mammalian cells: A general approach to site-specific insertion of amino acid analogues into proteins journal, November 2001

Progress toward the evolution of an organism with an expanded genetic code journal, April 1999

Comparative Protein Structure Modeling of Genes and Genomes journal, June 2000

A New Orthogonal Suppressor tRNA/Aminoacyl-tRNA Synthetase Pair for Evolving an Organism with an Expanded Genetic Code journal, September 2000

Site-specific incorporation of an unnatural amino acid into proteins in mammalian cells journal, November 2002

From genes to protein structure and function: novel applications of computational approaches in the genomic era journal, January 2000

Identity elements and aminoacylation of plant tRNATrp journal, November 1998

A general approach for the generation of orthogonal tRNAs journal, September 2001

In vivo incorporation of unnatural amino acids
patent, May 2006

In vivo incorporation of unnatural amino acids
patent-application, May 2003

Methods and compositions for the production of orthogonal tRNA-aminoacyltRNA synthetase pairs
patent-application, June 2003

Low brain uptake of L-[ ¹¹ C]5-hydroxytryptophan in major depression: a positron emission tomography study on patients and healthy volunteers
journal, June 1991

Twenty-first aminoacyl-tRNA synthetase-suppressor tRNA pairs for possible use in site-specific incorporation of amino acid analogues into proteins in eukaryotes and in eubacteria
journal, January 2001

An Expanded Eukaryotic Genetic Code
journal, August 2003

Cloning and nucleotide sequence of the structural gene coding for Bacillus subtilis tryptophanyl-tRNA synthetase
journal, December 1988

Aminoacyl-tRNA synthetases
journal, December 1997

Adding Amino Acids with Novel Reactivity to the Genetic Code of Saccharomyces Cerevisiae
journal, October 2003

Protein tolerance to random amino acid change
journal, June 2004

Characterization of aminoacyl-adenylates in B. tsubtilis tryptophanyl-tRNA synthetase, by the fluorescence of tryptophan analogs 5-hydroxytryptophan and 7-azatryptophan
journal, December 1993

The renaissance of aminoacyl-tRNA synthesis
journal, May 2001

A "Silent" Polymorphism in the MDR1 Gene Changes Substrate Specificity
journal, January 2007

Import of amber and ochre suppressor tRNAs into mammalian cells: A general approach to site-specific insertion of amino acid analogues into proteins
journal, November 2001

Progress toward the evolution of an organism with an expanded genetic code
journal, April 1999

Comparative Protein Structure Modeling of Genes and Genomes
journal, June 2000

A New Orthogonal Suppressor tRNA/Aminoacyl-tRNA Synthetase Pair for Evolving an Organism with an Expanded Genetic Code
journal, September 2000

Site-specific incorporation of an unnatural amino acid into proteins in mammalian cells
journal, November 2002

From genes to protein structure and function: novel applications of computational approaches in the genomic era
journal, January 2000

Identity elements and aminoacylation of plant tRNATrp
journal, November 1998

A general approach for the generation of orthogonal tRNAs
journal, September 2001