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Title: Microbial reductive dehalogenation of vinyl chloride

Abstract

Compositions and methods are provided that relate to the bioremediation of chlorinated ethenes, particularly the bioremediation of vinyl chloride by Dehalococcoides-like organisms. An isolated strain of bacteria, Dehalococcoides sp. strain VS, that metabolizes vinyl chloride is provided; the genetic sequence of the enzyme responsible for vinyl chloride dehalogenation; methods of assessing the capability of endogenous organisms at an environmental site to metabolize vinyl chloride; and a method of using the strains of the invention for bioremediation.

Inventors:
 [1];  [2];  [3];  [4];  [5];  [1]
  1. Stanford, CA
  2. Baltimore, MD
  3. Berlin, DE
  4. Mannheim, DE
  5. Los Angeles, CA
Issue Date:
Research Org.:
The Board of Trustees of the Leland Stanford Junior University, Palo Alto, CA, USA
Sponsoring Org.:
USDOE
OSTI Identifier:
1119695
Patent Number(s):
8,647,824
Application Number:
13/294,927
Assignee:
The Board of Trustees of the Leland Stanford Junior University (Palo Alto, CA)
DOE Contract Number:  
FG07-99ER62883
Resource Type:
Patent
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES

Citation Formats

Spormann, Alfred M, Muller, Jochen A, Rosner, Bettina M, Von Abendroth, Gregory, Meshulam-Simon, Galit, and McCarty, Perry L. Microbial reductive dehalogenation of vinyl chloride. United States: N. p., 2014. Web.
Spormann, Alfred M, Muller, Jochen A, Rosner, Bettina M, Von Abendroth, Gregory, Meshulam-Simon, Galit, & McCarty, Perry L. Microbial reductive dehalogenation of vinyl chloride. United States.
Spormann, Alfred M, Muller, Jochen A, Rosner, Bettina M, Von Abendroth, Gregory, Meshulam-Simon, Galit, and McCarty, Perry L. Tue . "Microbial reductive dehalogenation of vinyl chloride". United States. https://www.osti.gov/servlets/purl/1119695.
@article{osti_1119695,
title = {Microbial reductive dehalogenation of vinyl chloride},
author = {Spormann, Alfred M and Muller, Jochen A and Rosner, Bettina M and Von Abendroth, Gregory and Meshulam-Simon, Galit and McCarty, Perry L},
abstractNote = {Compositions and methods are provided that relate to the bioremediation of chlorinated ethenes, particularly the bioremediation of vinyl chloride by Dehalococcoides-like organisms. An isolated strain of bacteria, Dehalococcoides sp. strain VS, that metabolizes vinyl chloride is provided; the genetic sequence of the enzyme responsible for vinyl chloride dehalogenation; methods of assessing the capability of endogenous organisms at an environmental site to metabolize vinyl chloride; and a method of using the strains of the invention for bioremediation.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {2014},
month = {2}
}

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Works referenced in this record:

Growth of a Dehalococcoides-Like Microorganism on Vinyl Chloride and cis-Dichloroethene as Electron Acceptors as Determined by Competitive PCR
journal, February 2003


Detoxification of vinyl chloride to ethene coupled to growth of an anaerobic bacterium
journal, July 2003

  • He, Jianzhong; Ritalahti, Kirsti M.; Yang, Kun-Lin
  • Nature, Vol. 424, Issue 6944
  • DOI: 10.1038/nature01717

Molecular Analysis of Dehalococcoides 16S Ribosomal DNA from Chloroethene-Contaminated Sites throughout North America and Europe
journal, February 2002


Trichloroethene Reductive Dehalogenase from Dehalococcoides ethenogenes: Sequence of tceA and Substrate Range Characterization
journal, December 2000


Isolation of a Bacterium That Reductively Dechlorinates Tetrachloroethene to Ethene
journal, June 1997


Reductive Dechlorination of cis -1,2-Dichloroethene and Vinyl Chloride by “ Dehalococcoides ethenogenes
journal, February 2001

  • Maymó-Gatell, Xavier; Nijenhuis, Ivonne; Zinder, Stephen H.
  • Environmental Science & Technology, Vol. 35, Issue 3
  • DOI: 10.1021/es001285i

From genes to protein structure and function: novel applications of computational approaches in the genomic era
journal, January 2000