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Title: Microbial reductive dehalogenation of vinyl chloride

Abstract

Compositions and methods are provided that relate to the bioremediation of chlorinated ethenes, particularly the bioremediation of vinyl chloride by Dehalococcoides-like organisms. An isolated strain of bacteria, Dehalococcoides sp. strain VS, that metabolizes vinyl chloride is provided; the genetic sequence of the enzyme responsible for vinyl chloride dehalogenation; methods of assessing the capability of endogenous organisms at an environmental site to metabolize vinyl chloride; and a method of using the strains of the invention for bioremediation.

Inventors:
 [1];  [2];  [3];  [4];  [5];  [1]
  1. Stanford, CA
  2. Baltimore, MD
  3. Berlin, DE
  4. Mannheim, DE
  5. Los Angeles, CA
Issue Date:
Research Org.:
The Board of Trustees of the Leland Stanford Junior University, Palo Alto, CA, USA
Sponsoring Org.:
USDOE
OSTI Identifier:
1119695
Patent Number(s):
8647824
Application Number:
13/294,927
Assignee:
The Board of Trustees of the Leland Stanford Junior University (Palo Alto, CA)
Patent Classifications (CPCs):
B - PERFORMING OPERATIONS B09 - DISPOSAL OF SOLID WASTE B09C - RECLAMATION OF CONTAMINATED SOIL
C - CHEMISTRY C12 - BIOCHEMISTRY C12N - MICROORGANISMS OR ENZYMES
DOE Contract Number:  
FG07-99ER62883
Resource Type:
Patent
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES

Citation Formats

Spormann, Alfred M, Muller, Jochen A, Rosner, Bettina M, Von Abendroth, Gregory, Meshulam-Simon, Galit, and McCarty, Perry L. Microbial reductive dehalogenation of vinyl chloride. United States: N. p., 2014. Web.
Spormann, Alfred M, Muller, Jochen A, Rosner, Bettina M, Von Abendroth, Gregory, Meshulam-Simon, Galit, & McCarty, Perry L. Microbial reductive dehalogenation of vinyl chloride. United States.
Spormann, Alfred M, Muller, Jochen A, Rosner, Bettina M, Von Abendroth, Gregory, Meshulam-Simon, Galit, and McCarty, Perry L. Tue . "Microbial reductive dehalogenation of vinyl chloride". United States. https://www.osti.gov/servlets/purl/1119695.
@article{osti_1119695,
title = {Microbial reductive dehalogenation of vinyl chloride},
author = {Spormann, Alfred M and Muller, Jochen A and Rosner, Bettina M and Von Abendroth, Gregory and Meshulam-Simon, Galit and McCarty, Perry L},
abstractNote = {Compositions and methods are provided that relate to the bioremediation of chlorinated ethenes, particularly the bioremediation of vinyl chloride by Dehalococcoides-like organisms. An isolated strain of bacteria, Dehalococcoides sp. strain VS, that metabolizes vinyl chloride is provided; the genetic sequence of the enzyme responsible for vinyl chloride dehalogenation; methods of assessing the capability of endogenous organisms at an environmental site to metabolize vinyl chloride; and a method of using the strains of the invention for bioremediation.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {2014},
month = {2}
}

Works referenced in this record:

Growth of a Dehalococcoides-Like Microorganism on Vinyl Chloride and cis-Dichloroethene as Electron Acceptors as Determined by Competitive PCR
journal, February 2003


Detoxification of vinyl chloride to ethene coupled to growth of an anaerobic bacterium
journal, July 2003


Molecular Analysis of Dehalococcoides 16S Ribosomal DNA from Chloroethene-Contaminated Sites throughout North America and Europe
journal, February 2002


Trichloroethene Reductive Dehalogenase from Dehalococcoides ethenogenes: Sequence of tceA and Substrate Range Characterization
journal, December 2000


Reductive Dechlorination of cis -1,2-Dichloroethene and Vinyl Chloride by “ Dehalococcoides ethenogenes
journal, February 2001


From genes to protein structure and function: novel applications of computational approaches in the genomic era
journal, January 2000