Variant Hypocrea jercorina CBH1 cellulases

Day, Anthony; Goedegebuur, Frits; Gualfetti, Peter; Mitchinson, Colin; Neefe, Paulien; Sandgren, Mats; Shaw, Andrew; Stahlberg, Jerry

Title: Variant Hypocrea jercorina CBH1 cellulases

Abstract

Described herein are variants of H. jecorina CBH I, a Cel7 enzyme. The present invention provides novel cellobiohydrolases that have improved thermostability and reversibility.

Inventors:: Day, Anthony; Goedegebuur, Frits; Gualfetti, Peter; Mitchinson, Colin; Neefe, Paulien; Sandgren, Mats; Shaw, Andrew; Stahlberg, Jerry

Issue Date:: 2014-01-28

Research Org.:: Danisco US Inc., Palo Alto, CA (United States)

Sponsoring Org.:: USDOE

OSTI Identifier:: 1117874

Patent Number(s):: 8637294

Application Number:: 11/728,219

Assignee:: Danisco US Inc. (Palo Alto, CA)

Patent Classifications (CPCs):: C - CHEMISTRY C11 - ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES C11D - DETERGENT COMPOSITIONS

C - CHEMISTRY C12 - BIOCHEMISTRY C12N - MICROORGANISMS OR ENZYMES

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C - CHEMISTRY C11 - ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES C11D - DETERGENT COMPOSITIONS
C11D3/38645 - {containing cellulase}

C - CHEMISTRY C12 - BIOCHEMISTRY C12N - MICROORGANISMS OR ENZYMES
C12N9/2437 - {Cellulases

C - CHEMISTRY C12 - BIOCHEMISTRY C12P - FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE {
C12P19/02 - Monosaccharides
C12P19/14 - produced by the action of a carbohydrase {
C12P7/10 - substrate containing cellulosic material

C - CHEMISTRY C12 - BIOCHEMISTRY C12Y - ENZYMES
C12Y302/01091 - Cellulose 1,4-beta-cellobiosidase

D - TEXTILES D21 - PAPER-MAKING D21C - PRODUCTION OF CELLULOSE BY REMOVING NON-CELLULOSE SUBSTANCES FROM CELLULOSE-CONTAINING MATERIALS
D21C5/005 - {Treatment of cellulose-containing material with microorganisms or enzymes}

Y - NEW / CROSS SECTIONAL TECHNOLOGIES Y02 - TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE Y02E - REDUCTION OF GREENHOUSE GAS [GHG] EMISSIONS, RELATED TO ENERGY GENERATION, TRANSMISSION OR DISTRIBUTION
Y02E50/10 - Biofuels

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DOE Contract Number:: AC36-99GO10337

Resource Type:: Patent

Resource Relation:: Patent File Date: 2007 Mar 22

Country of Publication:: United States

Language:: English

Subject:: 59 BASIC BIOLOGICAL SCIENCES

Citation Formats


                    Day, Anthony, Goedegebuur, Frits, Gualfetti, Peter, Mitchinson, Colin, Neefe, Paulien, Sandgren, Mats, Shaw, Andrew, and Stahlberg, Jerry. Variant Hypocrea jercorina CBH1 cellulases.  United States: N. p., 2014. 
        Web.

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                    Day, Anthony, Goedegebuur, Frits, Gualfetti, Peter, Mitchinson, Colin, Neefe, Paulien, Sandgren, Mats, Shaw, Andrew, & Stahlberg, Jerry. Variant Hypocrea jercorina CBH1 cellulases.  United States.

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                    Day, Anthony, Goedegebuur, Frits, Gualfetti, Peter, Mitchinson, Colin, Neefe, Paulien, Sandgren, Mats, Shaw, Andrew, and Stahlberg, Jerry. Tue .  
        "Variant Hypocrea jercorina CBH1 cellulases".  United States.  https://www.osti.gov/servlets/purl/1117874.

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@article{osti_1117874,

  title        = {Variant Hypocrea jercorina CBH1 cellulases},

  author       = {Day, Anthony and Goedegebuur, Frits and Gualfetti, Peter and Mitchinson, Colin and Neefe, Paulien and Sandgren, Mats and Shaw, Andrew and Stahlberg, Jerry},

  abstractNote = {Described herein are variants of H. jecorina CBH I, a Cel7 enzyme. The present invention provides novel cellobiohydrolases that have improved thermostability and reversibility.},

  doi          = {},

  journal      = {},
number       = ,

  volume       = ,

  place        = {United States},

  year         = {2014},

  month        = {1}

}

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Production of Antigen-Specific Human Antibodies from Mice Engineered with Human Heavy and Light Chain YACs
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Jakobovits, Aya; Green, Larry L.; Hardy, Margaret C.
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Site-directed mutagenesis of the putative catalytic residues of Trichoderma reesci cellobiohydrolase I and endoglucanase I
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Mitsuishi, Yasushi; Nitisinprasert, Sunee; Saloheimo, Markku
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Comparison of family 12 glycoside hydrolases and recruited substitutions important for thermal stability
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Sandgren, Mats; Gualfetti, Peter J.; Shaw, Andrew
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Determinants of Enzyme Thermostability Observed in the Molecular Structure of Thermus aquaticus d -Glyceraldehyde-3-phosphate Dehydrogenase at 2.5 Å Resolution
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Tanner, John J.; Hecht, Ralph M.; Krause, Kurt L.
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Isolation of uridine auxotrophs from Trichoderma reesei and efficient transformation with the cloned ura3 and ura5 genes
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Bergès, Thierry; Barreau, Christian
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Saloheimo, M.; Lehtovaara, P.; Penttilä, M.
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Zuber, H.
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Molecular Biology of Cellulolytic Fungi
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Nevalainen, K. M. H.; Penttilä, M. E.
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Goedegebuur, Frits; Fowler, Timothy; Phillips, Jay
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Penttilä, Merja E.; André, Lars; Lehtovaara, Päivi
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Knowles, Jonathan; Lehtovaara, Päivi; Teeri, Tuula
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Urea and Thermal Equilibrium Denaturation Studies on the Dimerization Domain of Escherichia coli Trp Repressor
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Gloss, Lisa M.; Matthews, C. Robert
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Method for producing ascorbic acid intermediates
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Burley, S.; Petsko, G.
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Shoemaker, S. P.; Brown, R. D.
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Tomaz, Cândida T.; Queiroz, João A.
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Described herein are variants of H. jecorina CBH I, a Cel7 enzyme. The present invention provides novel cellobiohydrolases that have improved thermostability and reversibility.
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Disclosed are a number of homologs and variants of Hypocrea jecorina Cel7A (formerly Trichoderma reesei cellobiohydrolase I or CBH1), nucleic acids encoding the same and methods for producing the same. The homologs and variant cellulases have the amino acid sequence of a glycosyl hydrolase of family 7A wherein one or more amino acid residues are substituted and/or deleted.
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Title: Variant Hypocrea jercorina CBH1 cellulases

Abstract

Citation Formats

Ion-exchange chromatographic purification and quantitative analysis of Trichoderma reesei cellulases cellobiohydrolase I, II and endoglucanase II by fast protein liquid chromatography journal, May 1998

DNA sequence encoding endoglucanase III cellulase patent, December 1995

Identification of a fungal cutinase promoter that is inducible by a plant signal via a phosphorylated trans-acting factor. journal, September 1991

The roles and function of cellulose-binding domains journal, September 1997

Reshaping human antibodies: grafting an antilysozyme activity journal, March 1988

High-resolution crystal structures reveal how a cellulose chain is bound in the 50 Å long tunnel of cellobiohydrolase I from Trichoderma reesei journal, January 1998

Genomic organization of a cellulase gene family in Phanerochaete chrysosporium journal, November 1992

Production of fully human antibodies by transgenic mice journal, January 1995

Enhanced protein thermostability from site-directed mutations that decrease the entropy of unfolding. journal, October 1987

CelS: a novel endoglucanase identified from Erwinia carotovora subsp. carotovora journal, May 1990

Complete nucleotide sequence of a gene coding for Aspergillus aculeatus cellulase (FI-CMCase) journal, January 1990

Cloning and sequencing of cellulase cDNA from Aspergillus kawachii and its expression in Saccharomyces cerevisiae journal, April 1995

Response of a protein structure to cavity-creating mutations and its relation to the hydrophobic effect journal, January 1992

Secretion of Trichoderma reesei β-glucosidase by Saccharomyces cerevisiae journal, February 1996

Homologous domains in Trichoderma reesei cellulolytic enzymes: Gene sequence and expression of cellobiohydrolase II journal, January 1987

ACEII, a Novel Transcriptional Activator Involved in Regulation of Cellulase and Xylanase Genes of Trichoderma reesei journal, April 2001

Codon optimization of xylanase gene xynB from the thermophilic bacterium Dictyoglomus thermophilum for expression in the filamentous fungus Trichoderma reesei journal, September 2000

Exploitation of the cellulase enzyme complex of neurospora patent, September 1999

Methods for reducing the harshness of a cotton-containing fabric patent, July 1997

Fractionation of cellulase and β-glucosidase in a Trichoderma reesei culture liquid by use of two-phase partitioning journal, November 1999

Crystalline cellulose degradation: new insight into the function of cellobiohydrolases journal, May 1997

Properties of cellulolytic enzyme systems journal, April 1985

Specific quantification of trichoderma reesei cellulases in reconstituted mixtures and its application to cellulase-cellulose binding studies journal, October 1994

Cloning and Sequencing of a Rhodothermus marinus Gene, bglA, Coding for a Thermostable beta-Glucanase and its Expression in Escherichia coli journal, September 1994

Methods for stonewashing fabrics using endoglucanases patent, July 1997

Cloning and sequencing of the cDNA encoding β-glucosidase 1 from Aspergillus aculeatus journal, September 1996

Reshaping human antibodies for therapy journal, March 1988

Molecular Cloning of Exo–Cellobiohydrolase I Derived from Trichoderma Reesei Strain L27 journal, October 1983

Transformation of Aspergillus nidulans by using a trpC plasmid. journal, March 1984

Improved transformation efficiency of Aspergillus niger using the homologous niaD gene for nitrate reductase journal, July 1989

Penicillopepsin-JT2, a recombinant enzyme from Penicillium janthinellum and the contribution of a hydrogen bond in subsite S 3 to k cat journal, January 2000

Improved oligonudeotide site-directed rautagenesis using M13 vectors journal, January 1985

Fungal cellulase composition containing alkaline CMC-endoglucanase and essentially no cellobiohydrolase and method of making thereof patent, July 1998

[57] Rethinking your purification procedure book, January 1990

Continuous cultures of fused cells secreting antibody of predefined specificity journal, August 1975

Simultaneous saccharification and fermentation of lignocellulosic wastes to ethanol using a thermotolerant yeast journal, April 2001

Basic local alignment search tool journal, October 1990

Cloning, sequencing and overexpression of a Rhodothermus marinus gene encoding a thermostable cellulase of glycosyl hydrolase family 12 journal, March 1998

Comparison of biosequences journal, December 1981

Trichoderma reesei cellobiohydrolase I with an endoglucanase cellulose-binding domain: action on bacterial microcrystalline cellulose journal, September 1997

Synthesis and secretion of wheat α-amylase in Saccharomyces cerevisiae journal, January 1987

Detection of a Stable Intermediate in the Thermal Unfolding of a Cysteine-Free Form of Dihydrofolate Reductase from Escherichia Coli journal, August 1995

Trichoderma reesei cellulases and their core domains in the hydrolysis and modification of chemical pulp journal, June 2000

Structural and Genetic Analysis of Protein Stability journal, June 1993

Construction of mutant and chimeric genes using the polymerase chain reaction journal, January 1989

Method for treating cotton-containing fabric with a cellulase composition containing endoglucanase components and which composition is free of exo-cellobiohydrolase I patent, September 1993

Recombinant immunoglobin preparations patent, March 1989

Multiple Proline Substitutions Cumulatively Thermostabilize Bacillus Cereus ATCC7064 Oligo-1,6-Glucosidase. Irrefragable Proof Supporting the Proline Rule journal, December 1994

A New Model For Enzymatic Hydrolysis of Cellulose Based on the Two-Domain Structure of Cellobiohydrolase I journal, March 1991

Advanced Bioethanol Production Technologies: A Perspective book, May 1997

Structure of Cellulases and Their Applications journal, April 1997

Amino acid substitution matrices from protein blocks. journal, November 1992

Molecular evidence that the asexual industrial fungus Trichoderma reesei is a clonal derivative of the ascomycete Hypocrea jecorina. journal, July 1996

Nucleotide sequence of the cellobiohydrolase gene from Trichoderma viride journal, January 1990

Detergent composition for clothing incorporating a cellulase patent, April 1989

A versatile transformation system for the cellulolytic filamentous fungus Trichoderma reesei journal, January 1987

Studies of the cellulolytic system of Trichoderma reesei QM 9414. Analysis of domain function in two cellobiohydrolases by limited proteolysis journal, January 1988

Characteristics of fungal cellulases journal, January 1991

Fungal cellulase composition containing alkaline CMC-endoglucanase and essentially no cellobiohydrolase patent, November 1997

Cassette mutagenesis: an efficient method for generation of multiple mutations at defined sites journal, January 1985

Conjugative transfer of a shuttle plasmid for Escherichia coli to Mycobacterium smegmatis journal, May 1990

Engineering thermostability: lessons from thermophilic proteins journal, January 1995

Separation of endo- and exo-type cellulases using a new affinity chromatography method journal, April 1984

Characterization of cellulases by HPLC separation journal, January 1983

Cellulase enzymes and systems for their expressions patent, January 1999

Engineering of a glycosidase Family 7 cellobiohydrolase to more alkaline pH optimum: the pH behaviour of Trichoderma reesei Cel7A and its E223S/ A224H/L225V/T226A/D262G mutant journal, May 2001

Detergent cellulase patent, March 1984

Purification and characterization of a β-glucosidase from solid-state cultures of Humicola grisea var. thermoidea journal, January 1996

Transformation of Trichoderma harzianum by high-voltage electric pulse journal, February 1990

Enzymes, Energy, and the Environment: A Strategic Perspective on the U.S. Department of Energy's Research and Development Activities for Bioethanol journal, October 1999

Randomization of genes by PCR mutagenesis. journal, August 1992

Antibodies specific for the human retinoblastoma protein identify a family of related polypeptides. journal, November 1991

Detergent compositions containing cellulase compositions deficient in CBH I type components patent, December 2000

Oligosaccharide specificity of a family 7 endoglucanase: insertion of potential sugar-binding subsites journal, September 1997

Use of Aspergillus overproducing mutants, cured for intergrated plasmid, to overproduce heterologous proteins journal, August 1993

Expression of two Trichoderma reesei endoglucanases in the yeast Saccharomyces cerevisiae journal, September 1987

Contribution of hydrophobic interactions to protein stability journal, June 1988

Ion-exchange chromatographic purification and quantitative analysis of Trichoderma reesei cellulases cellobiohydrolase I, II and endoglucanase II by fast protein liquid chromatography
journal, May 1998

DNA sequence encoding endoglucanase III cellulase
patent, December 1995

Identification of a fungal cutinase promoter that is inducible by a plant signal via a phosphorylated trans-acting factor.
journal, September 1991

The roles and function of cellulose-binding domains
journal, September 1997

Reshaping human antibodies: grafting an antilysozyme activity
journal, March 1988

High-resolution crystal structures reveal how a cellulose chain is bound in the 50 Å long tunnel of cellobiohydrolase I from Trichoderma reesei
journal, January 1998

Genomic organization of a cellulase gene family in Phanerochaete chrysosporium
journal, November 1992

Production of fully human antibodies by transgenic mice
journal, January 1995

Enhanced protein thermostability from site-directed mutations that decrease the entropy of unfolding.
journal, October 1987

CelS: a novel endoglucanase identified from Erwinia carotovora subsp. carotovora
journal, May 1990

Complete nucleotide sequence of a gene coding for Aspergillus aculeatus cellulase (FI-CMCase)
journal, January 1990

Cloning and sequencing of cellulase cDNA from Aspergillus kawachii and its expression in Saccharomyces cerevisiae
journal, April 1995

Response of a protein structure to cavity-creating mutations and its relation to the hydrophobic effect
journal, January 1992

Secretion of Trichoderma reesei β-glucosidase by Saccharomyces cerevisiae
journal, February 1996

Homologous domains in Trichoderma reesei cellulolytic enzymes: Gene sequence and expression of cellobiohydrolase II
journal, January 1987

ACEII, a Novel Transcriptional Activator Involved in Regulation of Cellulase and Xylanase Genes of Trichoderma reesei
journal, April 2001

Codon optimization of xylanase gene xynB from the thermophilic bacterium Dictyoglomus thermophilum for expression in the filamentous fungus Trichoderma reesei
journal, September 2000

Exploitation of the cellulase enzyme complex of neurospora
patent, September 1999

Methods for reducing the harshness of a cotton-containing fabric
patent, July 1997

Fractionation of cellulase and β-glucosidase in a Trichoderma reesei culture liquid by use of two-phase partitioning
journal, November 1999

Crystalline cellulose degradation: new insight into the function of cellobiohydrolases
journal, May 1997

Properties of cellulolytic enzyme systems
journal, April 1985

Specific quantification of trichoderma reesei cellulases in reconstituted mixtures and its application to cellulase-cellulose binding studies
journal, October 1994

Cloning and Sequencing of a Rhodothermus marinus Gene, bglA, Coding for a Thermostable beta-Glucanase and its Expression in Escherichia coli
journal, September 1994

Methods for stonewashing fabrics using endoglucanases
patent, July 1997

Cloning and sequencing of the cDNA encoding β-glucosidase 1 from Aspergillus aculeatus
journal, September 1996

Reshaping human antibodies for therapy
journal, March 1988

Molecular Cloning of Exo–Cellobiohydrolase I Derived from Trichoderma Reesei Strain L27
journal, October 1983

Transformation of Aspergillus nidulans by using a trpC plasmid.
journal, March 1984

Improved transformation efficiency of Aspergillus niger using the homologous niaD gene for nitrate reductase
journal, July 1989

Penicillopepsin-JT2, a recombinant enzyme from Penicillium janthinellum and the contribution of a hydrogen bond in subsite S ₃ to k _cat
journal, January 2000

Improved oligonudeotide site-directed rautagenesis using M13 vectors
journal, January 1985

Fungal cellulase composition containing alkaline CMC-endoglucanase and essentially no cellobiohydrolase and method of making thereof
patent, July 1998

[57] Rethinking your purification procedure
book, January 1990

Continuous cultures of fused cells secreting antibody of predefined specificity
journal, August 1975

Simultaneous saccharification and fermentation of lignocellulosic wastes to ethanol using a thermotolerant yeast
journal, April 2001

Basic local alignment search tool
journal, October 1990

Cloning, sequencing and overexpression of a Rhodothermus marinus gene encoding a thermostable cellulase of glycosyl hydrolase family 12
journal, March 1998

Comparison of biosequences
journal, December 1981

Trichoderma reesei cellobiohydrolase I with an endoglucanase cellulose-binding domain: action on bacterial microcrystalline cellulose
journal, September 1997

Synthesis and secretion of wheat α-amylase in Saccharomyces cerevisiae
journal, January 1987

Detection of a Stable Intermediate in the Thermal Unfolding of a Cysteine-Free Form of Dihydrofolate Reductase from Escherichia Coli
journal, August 1995

Trichoderma reesei cellulases and their core domains in the hydrolysis and modification of chemical pulp
journal, June 2000

Structural and Genetic Analysis of Protein Stability
journal, June 1993

Construction of mutant and chimeric genes using the polymerase chain reaction
journal, January 1989

Method for treating cotton-containing fabric with a cellulase composition containing endoglucanase components and which composition is free of exo-cellobiohydrolase I
patent, September 1993

Recombinant immunoglobin preparations
patent, March 1989

Multiple Proline Substitutions Cumulatively Thermostabilize Bacillus Cereus ATCC7064 Oligo-1,6-Glucosidase. Irrefragable Proof Supporting the Proline Rule
journal, December 1994

A New Model For Enzymatic Hydrolysis of Cellulose Based on the Two-Domain Structure of Cellobiohydrolase I
journal, March 1991

Advanced Bioethanol Production Technologies: A Perspective
book, May 1997

Structure of Cellulases and Their Applications
journal, April 1997

Amino acid substitution matrices from protein blocks.
journal, November 1992

Molecular evidence that the asexual industrial fungus Trichoderma reesei is a clonal derivative of the ascomycete Hypocrea jecorina.
journal, July 1996

Nucleotide sequence of the cellobiohydrolase gene from Trichoderma viride
journal, January 1990

Detergent composition for clothing incorporating a cellulase
patent, April 1989

A versatile transformation system for the cellulolytic filamentous fungus Trichoderma reesei
journal, January 1987

Studies of the cellulolytic system of Trichoderma reesei QM 9414. Analysis of domain function in two cellobiohydrolases by limited proteolysis
journal, January 1988

Characteristics of fungal cellulases
journal, January 1991

Fungal cellulase composition containing alkaline CMC-endoglucanase and essentially no cellobiohydrolase
patent, November 1997

Cassette mutagenesis: an efficient method for generation of multiple mutations at defined sites
journal, January 1985

Conjugative transfer of a shuttle plasmid for Escherichia coli to Mycobacterium smegmatis
journal, May 1990

Engineering thermostability: lessons from thermophilic proteins
journal, January 1995

Separation of endo- and exo-type cellulases using a new affinity chromatography method
journal, April 1984

Characterization of cellulases by HPLC separation
journal, January 1983

Cellulase enzymes and systems for their expressions
patent, January 1999

Engineering of a glycosidase Family 7 cellobiohydrolase to more alkaline pH optimum: the pH behaviour of Trichoderma reesei Cel7A and its E223S/ A224H/L225V/T226A/D262G mutant
journal, May 2001

Detergent cellulase
patent, March 1984

Purification and characterization of a β-glucosidase from solid-state cultures of Humicola grisea var. thermoidea
journal, January 1996

Transformation of Trichoderma harzianum by high-voltage electric pulse
journal, February 1990

Enzymes, Energy, and the Environment: A Strategic Perspective on the U.S. Department of Energy's Research and Development Activities for Bioethanol
journal, October 1999

Randomization of genes by PCR mutagenesis.
journal, August 1992

Antibodies specific for the human retinoblastoma protein identify a family of related polypeptides.
journal, November 1991

Detergent compositions containing cellulase compositions deficient in CBH I type components
patent, December 2000

Oligosaccharide specificity of a family 7 endoglucanase: insertion of potential sugar-binding subsites
journal, September 1997

Use of Aspergillus overproducing mutants, cured for intergrated plasmid, to overproduce heterologous proteins
journal, August 1993

Expression of two Trichoderma reesei endoglucanases in the yeast Saccharomyces cerevisiae
journal, September 1987

Contribution of hydrophobic interactions to protein stability
journal, June 1988

Rapid and efficient site-specific mutagenesis without phenotypic selection.
journal, January 1985

Improved tools for biological sequence comparison.
journal, April 1988

Production of Antigen-Specific Human Antibodies from Mice Engineered with Human Heavy and Light Chain YACs
journal, September 1995