Production of carrier-peptide conjugates using chemically reactive unnatural amino acids
Abstract
Provided are methods of making carrier polypeptide that include incorporating a first unnatural amino acid into a carrier polypeptide variant, incorporating a second unnatural amino acid into a target polypeptide variant, and reacting the first and second unnatural amino acids to produce the conjugate. Conjugates produced using the provided methods are also provided. In addition, orthogonal translation systems in methylotrophic yeast and methods of using these systems to produce carrier and target polypeptide variants comprising unnatural amino acids are provided.
- Inventors:
- Issue Date:
- Research Org.:
- The Scripps Research Institute, La Jolla, CA, USA
- Sponsoring Org.:
- USDOE
- OSTI Identifier:
- 1117872
- Patent Number(s):
- 8637306
- Application Number:
- 12/653,274
- Assignee:
- The Scripps Research Institute (La Jolla, CA)
- Patent Classifications (CPCs):
-
C - CHEMISTRY C07 - ORGANIC CHEMISTRY C07K - PEPTIDES
C - CHEMISTRY C12 - BIOCHEMISTRY C12N - MICROORGANISMS OR ENZYMES
- DOE Contract Number:
- FG03-00ER46051
- Resource Type:
- Patent
- Resource Relation:
- Patent File Date: 2009 Dec 09
- Country of Publication:
- United States
- Language:
- English
- Subject:
- 37 INORGANIC, ORGANIC, PHYSICAL, AND ANALYTICAL CHEMISTRY
Citation Formats
Young, Travis, and Schultz, Peter G. Production of carrier-peptide conjugates using chemically reactive unnatural amino acids. United States: N. p., 2014.
Web.
Young, Travis, & Schultz, Peter G. Production of carrier-peptide conjugates using chemically reactive unnatural amino acids. United States.
Young, Travis, and Schultz, Peter G. Tue .
"Production of carrier-peptide conjugates using chemically reactive unnatural amino acids". United States. https://www.osti.gov/servlets/purl/1117872.
@article{osti_1117872,
title = {Production of carrier-peptide conjugates using chemically reactive unnatural amino acids},
author = {Young, Travis and Schultz, Peter G},
abstractNote = {Provided are methods of making carrier polypeptide that include incorporating a first unnatural amino acid into a carrier polypeptide variant, incorporating a second unnatural amino acid into a target polypeptide variant, and reacting the first and second unnatural amino acids to produce the conjugate. Conjugates produced using the provided methods are also provided. In addition, orthogonal translation systems in methylotrophic yeast and methods of using these systems to produce carrier and target polypeptide variants comprising unnatural amino acids are provided.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {2014},
month = {1}
}
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