Title: Group specific internal standard technology (GSIST) for simultaneous identification and quantification of small molecules

Abstract

Reagents and methods are provided that permit simultaneous analysis of multiple diverse small molecule analytes present in a complex mixture. Samples are labeled with chemically identical but isotopically distince forms of the labeling reagent, and analyzed using mass spectrometry. A single reagent simultaneously derivatizes multiple small molecule analytes having different reactive functional groups.

Inventors:

Adamec, Jiri; Yang, Wen-Chu; Regnier, Fred E

Issue Date:

2014-01-14

Research Org.:

Purdue Research Foundation, West Lafayette, IN, USA

Sponsoring Org.:

USDOE

OSTI Identifier:

1117679

Patent Number(s):

8628977

Application Number:

12/990,560

Assignee:

Purdue Research Foundation (West Lafayette, IN)

Patent Classifications (CPCs):

G - PHYSICS G01 - MEASURING G01N - INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES

Y - NEW / CROSS SECTIONAL TECHNOLOGIES Y10 - TECHNICAL SUBJECTS COVERED BY FORMER USPC Y10T - TECHNICAL SUBJECTS COVERED BY FORMER US CLASSIFICATION

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G - PHYSICS G01 - MEASURING G01N - INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES

G01N33/6842 - {Proteomic analysis of subsets of protein mixtures with reduced complexity, e.g. membrane proteins, phosphoproteins, organelle proteins}

Y - NEW / CROSS SECTIONAL TECHNOLOGIES Y10 - TECHNICAL SUBJECTS COVERED BY FORMER USPC Y10T - TECHNICAL SUBJECTS COVERED BY FORMER US CLASSIFICATION

Y10T436/17 - Nitrogen containing
Y10T436/13 - Tracers or tags
Y10T436/143333 - Saccharide [e.g., DNA, etc.]
Y10T436/24 - Nuclear magnetic resonance, electron spin resonance or other spin effects or mass spectrometry
Y10T436/10 - Composition for standardization, calibration, simulation, stabilization, preparation or preservation

G - PHYSICS G01 - MEASURING G01N - INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES

G01N33/6845 - {Methods of identifying protein-protein interactions in protein mixtures}

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DOE Contract Number:  

FC36-07GO17059

Resource Type:

Patent

Resource Relation:

Patent File Date: 2009 May 01

Country of Publication:

United States

Language:

English

Subject:

37 INORGANIC, ORGANIC, PHYSICAL, AND ANALYTICAL CHEMISTRY