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Title: Xylose utilization in recombinant Zymomonas

Abstract

Zymomonas expressing xylose isomerase from A. missouriensis was found to have improved xylose utilization, growth, and ethanol production when grown in media containing xylose. Xylose isomerases related to that of A. missouriensis were identified structurally through molecular phylogenetic and Profile Hidden Markov Model analyses, providing xylose isomerases that may be used to improve xylose utilization.

Inventors:
; ; ; ;
Issue Date:
Research Org.:
E I du Pont de Nemours and Company, Wilmington, DE, USA
Sponsoring Org.:
USDOE
OSTI Identifier:
1117010
Patent Number(s):
8623623
Application Number:
13/161,749
Assignee:
E I du Pont de Nemours and Company (Wilmington, DE)
Patent Classifications (CPCs):
C - CHEMISTRY C12 - BIOCHEMISTRY C12N - MICROORGANISMS OR ENZYMES
C - CHEMISTRY C12 - BIOCHEMISTRY C12P - FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE {
DOE Contract Number:  
FC36-07GO17056
Resource Type:
Patent
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES

Citation Formats

Kahsay, Robel Y, Qi, Min, Tao, Luan, Viitanen, Paul V, and Yang, Jianjun. Xylose utilization in recombinant Zymomonas. United States: N. p., 2013. Web.
Kahsay, Robel Y, Qi, Min, Tao, Luan, Viitanen, Paul V, & Yang, Jianjun. Xylose utilization in recombinant Zymomonas. United States.
Kahsay, Robel Y, Qi, Min, Tao, Luan, Viitanen, Paul V, and Yang, Jianjun. Mon . "Xylose utilization in recombinant Zymomonas". United States. https://www.osti.gov/servlets/purl/1117010.
@article{osti_1117010,
title = {Xylose utilization in recombinant Zymomonas},
author = {Kahsay, Robel Y and Qi, Min and Tao, Luan and Viitanen, Paul V and Yang, Jianjun},
abstractNote = {Zymomonas expressing xylose isomerase from A. missouriensis was found to have improved xylose utilization, growth, and ethanol production when grown in media containing xylose. Xylose isomerases related to that of A. missouriensis were identified structurally through molecular phylogenetic and Profile Hidden Markov Model analyses, providing xylose isomerases that may be used to improve xylose utilization.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {Mon Jan 07 00:00:00 EST 2013},
month = {Mon Jan 07 00:00:00 EST 2013}
}

Works referenced in this record:

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Pentose fermentation by recombinant zymomonas
patent, January 1998


Zymomonas with improved xylose utilization
patent, August 2011


Single zymomonas mobilis strain for xylose and arabinose fermentation
patent, December 1998


Ethanol production using xylitol synthesis mutant of xylose-utilizing zymomonas
patent, June 2010


Recombinant zymomonas for pentose fermentation
patent, May 1996


Zymomonas with improved ethanol production in medium containing concentrated sugars and acetate
patent, September 2010


Ethanol production in fermentation of mixed sugars containing xylose
patent, December 2009


Restoration of a Defective Lactococcus lactis Xylose Isomerase
journal, July 2004


Zymomonas pentose-sugar fermenting strains and uses thereof
patent, May 2007


Xylose isomerase with improved properties
patent, November 2002


Xylitol synthesis mutant of xylose-utilizing zymomonas for ethanol production
patent, June 2010


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Metabolic Engineering of a Pentose Metabolism Pathway in Ethanologenic Zymomonas mobilis
journal, January 1995


High expression Zymomonas promoters
patent, August 2011


Expression of cloned Xanthomonasd-xylose catabolic genes in Zymomonas mobilis
journal, January 1988


Zymomonas with Improved Arabinose Utilization
patent-application, June 2011


Zymomonas with Improved Xylose Utilizations in Stress Conditions
patent-application, January 2011