Genetically modified yeast species, and fermentation processes using genetically modified yeast

Rajgarhia, Vineet; Koivuranta, Kari; Penttila, Merja; Ilmen, Marja; Suominen, Pirkko; Aristidou, Aristos; Miller, Christopher Kenneth; Olson, Stacey; Ruohonen, Laura

Title: Genetically modified yeast species, and fermentation processes using genetically modified yeast

Abstract

Yeast cells are transformed with an exogenous xylose isomerase gene. Additional genetic modifications enhance the ability of the transformed cells to ferment xylose to ethanol or other desired fermentation products. Those modifications include deletion of non-specific aldose reductase gene(s), deletion of xylitol dehydrogenase gene(s) and/or overexpression of xylulokinase.

Inventors:

Rajgarhia, Vineet ^[1]; Koivuranta, Kari ^[2]; Penttila, Merja ^[2]; Ilmen, Marja ^[2]; Suominen, Pirkko ^[3]; Aristidou, Aristos ^[3]; Miller, Christopher Kenneth ^[4]; Olson, Stacey ^[5]; Ruohonen, Laura ^[2]

Kingsport, TN
Helsinki, FI
Maple Grove, MN
Cottage Grove, MN
St. Bonifacius, MN

Issue Date:: 2014-01-07

Research Org.:: Cargill Incorporated, Wayzata, MN, USA

Sponsoring Org.:: USDOE

OSTI Identifier:: 1113979

Patent Number(s):: 8623633

Application Number:: 13/107,882

Assignee:: Cargill Incorporated (Wayzata, MN)

Patent Classifications (CPCs):: C - CHEMISTRY C12 - BIOCHEMISTRY C12N - MICROORGANISMS OR ENZYMES

C - CHEMISTRY C12 - BIOCHEMISTRY C12P - FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE {

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C - CHEMISTRY C12 - BIOCHEMISTRY C12N - MICROORGANISMS OR ENZYMES
C12N9/248 - {Xylanases}

C - CHEMISTRY C12 - BIOCHEMISTRY C12P - FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE {
C12P7/56 - Lactic acid

C - CHEMISTRY C12 - BIOCHEMISTRY C12Y - ENZYMES
C12Y101/01009 - D-Xylulose reductase
C12Y401/01001 - Pyruvate decarboxylase

C - CHEMISTRY C12 - BIOCHEMISTRY C12N - MICROORGANISMS OR ENZYMES
C12N15/81 - for yeasts
C12N9/0006 - {acting on CH-OH groups as donors

Y - NEW / CROSS SECTIONAL TECHNOLOGIES Y02 - TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE Y02E - REDUCTION OF GREENHOUSE GAS [GHG] EMISSIONS, RELATED TO ENERGY GENERATION, TRANSMISSION OR DISTRIBUTION
Y02E50/10 - Biofuels

C - CHEMISTRY C12 - BIOCHEMISTRY C12N - MICROORGANISMS OR ENZYMES
C12N9/88 - Lyases

C - CHEMISTRY C12 - BIOCHEMISTRY C12P - FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE {
C12P7/14 - Multiple stages of fermentation

C - CHEMISTRY C12 - BIOCHEMISTRY C12Y - ENZYMES
C12Y207/02003 - Phosphoglycerate kinase
C12Y207/01017 - Xylulokinase

C - CHEMISTRY C12 - BIOCHEMISTRY C12P - FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE {
C12P7/06 - Ethanol, i.e. non-beverage

C - CHEMISTRY C12 - BIOCHEMISTRY C12N - MICROORGANISMS OR ENZYMES
C12N9/92 - Glucose isomerase {

C - CHEMISTRY C12 - BIOCHEMISTRY C12Y - ENZYMES
C12Y501/03002 - UDP-glucose 4-epimerase

C - CHEMISTRY C12 - BIOCHEMISTRY C12N - MICROORGANISMS OR ENZYMES
C12N1/16 - Yeasts

C - CHEMISTRY C12 - BIOCHEMISTRY C12Y - ENZYMES
C12Y302/01037 - Xylan 1,4-beta-xylosidase

C - CHEMISTRY C12 - BIOCHEMISTRY C12N - MICROORGANISMS OR ENZYMES
C12N9/1205 - {Phosphotransferases with an alcohol group as acceptor

Y - NEW / CROSS SECTIONAL TECHNOLOGIES Y02 - TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE Y02P - CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
Y02P20/52 - using catalysts, e.g. selective catalysts

C - CHEMISTRY C12 - BIOCHEMISTRY C12N - MICROORGANISMS OR ENZYMES
C12N9/1217 - {Phosphotransferases with a carboxyl group as acceptor
C12N15/815 - {for yeasts other than Saccharomyces}

C - CHEMISTRY C12 - BIOCHEMISTRY C12Y - ENZYMES
C12Y503/01005 - Xylose isomerase

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DOE Contract Number:: FC07-021D14349

Resource Type:: Patent

Resource Relation:: Patent File Date: 2011 May 14

Country of Publication:: United States

Language:: English

Subject:: 60 APPLIED LIFE SCIENCES

Citation Formats


                    Rajgarhia, Vineet, Koivuranta, Kari, Penttila, Merja, Ilmen, Marja, Suominen, Pirkko, Aristidou, Aristos, Miller, Christopher Kenneth, Olson, Stacey, and Ruohonen, Laura. Genetically modified yeast species, and fermentation processes using genetically modified yeast.  United States: N. p., 2014. 
        Web.

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                    Rajgarhia, Vineet, Koivuranta, Kari, Penttila, Merja, Ilmen, Marja, Suominen, Pirkko, Aristidou, Aristos, Miller, Christopher Kenneth, Olson, Stacey, & Ruohonen, Laura. Genetically modified yeast species, and fermentation processes using genetically modified yeast.  United States.

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                    Rajgarhia, Vineet, Koivuranta, Kari, Penttila, Merja, Ilmen, Marja, Suominen, Pirkko, Aristidou, Aristos, Miller, Christopher Kenneth, Olson, Stacey, and Ruohonen, Laura. Tue .  
        "Genetically modified yeast species, and fermentation processes using genetically modified yeast".  United States.  https://www.osti.gov/servlets/purl/1113979.

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@article{osti_1113979,

  title        = {Genetically modified yeast species, and fermentation processes using genetically modified yeast},

  author       = {Rajgarhia, Vineet and Koivuranta, Kari and Penttila, Merja and Ilmen, Marja and Suominen, Pirkko and Aristidou, Aristos and Miller, Christopher Kenneth and Olson, Stacey and Ruohonen, Laura},

  abstractNote = {Yeast cells are transformed with an exogenous xylose isomerase gene. Additional genetic modifications enhance the ability of the transformed cells to ferment xylose to ethanol or other desired fermentation products. Those modifications include deletion of non-specific aldose reductase gene(s), deletion of xylitol dehydrogenase gene(s) and/or overexpression of xylulokinase.},

  doi          = {},

  journal      = {},
number       = ,

  volume       = ,

  place        = {United States},

  year         = {2014},

  month        = {1}

}

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Works referenced in this record:

Deletion of the GRE3 Aldose Reductase Gene and Its Influence on Xylose Metabolism in Recombinant Strains of Saccharomyces cerevisiae Expressing the xylA and XKS1 Genes
journal, December 2001

Traff, K. L.; Otero Cordero, R. R.; van Zyl, W. H.
Applied and Environmental Microbiology, Vol. 67, Issue 12
https://doi.org/10.1128/AEM.67.12.5668-5674.2001

Yeast vectors for integration at the HO locus
journal, June 2001

Voth, W. P.
Nucleic Acids Research, Vol. 29, Issue 12
https://doi.org/10.1093/nar/29.12.e59

High-level functional expression of a fungal xylose isomerase: the key to efficient ethanolic fermentation of xylose by ?
journal, October 2003

Kuyper, M.; Harhangi, H.; Stave, A.
FEMS Yeast Research, Vol. 4, Issue 1
https://doi.org/10.1016/S1567-1356(03)00141-7

Minimal metabolic engineering of for efficient anaerobic xylose fermentation a proof of principle
journal, March 2004

Kuyper, Marco; Winkler, Aaron; van Dijken, Johannes
FEMS Yeast Research, Vol. 4, Issue 6, p. 655-664
https://doi.org/10.1016/j.femsyr.2004.01.003

Xylose isomerase activity influences xylose fermentation with recombinant Saccharomyces cerevisiae strains expressing mutated xylA from Thermus thermophilus
journal, April 2003

Lönn, A.; Träff-Bjerre, K. L.; Cordero Otero, R. R.
Enzyme and Microbial Technology, Vol. 32, Issue 5
https://doi.org/10.1016/S0141-0229(03)00024-3

Xylulokinase Overexpression in Two Strains of Saccharomyces cerevisiae Also Expressing Xylose Reductase and Xylitol Dehydrogenase and Its Effect on Fermentation of Xylose and Lignocellulosic Hydrolysate
journal, September 2001

Johansson, B.; Christensson, C.; Hobley, T.
Applied and Environmental Microbiology, Vol. 67, Issue 9
https://doi.org/10.1128/AEM.67.9.4249-4255.2001

Amino Acid Substitutions in the Yeast Pichia Stipitis Xylitol Dehydrogenase Coenzyme-Binding Domain Affect the Coenzyme Specificity
journal, February 1995

Metzger, Markus H.; Hollenberg, Cornells P.
European Journal of Biochemistry, Vol. 228, Issue 1
https://doi.org/10.1111/j.1432-1033.1995.0050o.x

Xylose metabolism in the anaerobic fungus Piromyces sp. strain E2 follows the bacterial pathway
journal, June 2003

Harhangi, Harry R.; Akhmanova, Anna S.; Emmens, Roul
Archives of Microbiology, Vol. 180, Issue 2
https://doi.org/10.1007/s00203-003-0565-0

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Title: Genetically modified yeast species, and fermentation processes using genetically modified yeast

Abstract

Citation Formats

Deletion of the GRE3 Aldose Reductase Gene and Its Influence on Xylose Metabolism in Recombinant Strains of Saccharomyces cerevisiae Expressing the xylA and XKS1 Genes journal, December 2001

Yeast vectors for integration at the HO locus journal, June 2001

High-level functional expression of a fungal xylose isomerase: the key to efficient ethanolic fermentation of xylose by ? journal, October 2003

Minimal metabolic engineering of for efficient anaerobic xylose fermentation a proof of principle journal, March 2004

Xylose isomerase activity influences xylose fermentation with recombinant Saccharomyces cerevisiae strains expressing mutated xylA from Thermus thermophilus journal, April 2003

Xylulokinase Overexpression in Two Strains of Saccharomyces cerevisiae Also Expressing Xylose Reductase and Xylitol Dehydrogenase and Its Effect on Fermentation of Xylose and Lignocellulosic Hydrolysate journal, September 2001

Amino Acid Substitutions in the Yeast Pichia Stipitis Xylitol Dehydrogenase Coenzyme-Binding Domain Affect the Coenzyme Specificity journal, February 1995

Xylose metabolism in the anaerobic fungus Piromyces sp. strain E2 follows the bacterial pathway journal, June 2003

Deletion of the GRE3 Aldose Reductase Gene and Its Influence on Xylose Metabolism in Recombinant Strains of Saccharomyces cerevisiae Expressing the xylA and XKS1 Genes
journal, December 2001

Yeast vectors for integration at the HO locus
journal, June 2001

High-level functional expression of a fungal xylose isomerase: the key to efficient ethanolic fermentation of xylose by ?
journal, October 2003

Minimal metabolic engineering of for efficient anaerobic xylose fermentation a proof of principle
journal, March 2004

Xylose isomerase activity influences xylose fermentation with recombinant Saccharomyces cerevisiae strains expressing mutated xylA from Thermus thermophilus
journal, April 2003

Xylulokinase Overexpression in Two Strains of Saccharomyces cerevisiae Also Expressing Xylose Reductase and Xylitol Dehydrogenase and Its Effect on Fermentation of Xylose and Lignocellulosic Hydrolysate
journal, September 2001

Amino Acid Substitutions in the Yeast Pichia Stipitis Xylitol Dehydrogenase Coenzyme-Binding Domain Affect the Coenzyme Specificity
journal, February 1995

Xylose metabolism in the anaerobic fungus Piromyces sp. strain E2 follows the bacterial pathway
journal, June 2003