Fatty acid-producing hosts

Pfleger, Brian F; Lennen, Rebecca M

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Title: Fatty acid-producing hosts

Abstract

Described are hosts for overproducing a fatty acid product such as a fatty acid. The hosts include an exogenous nucleic acid encoding a thioesterase and, optionally, an exogenous nucleic acid encoding an acetyl-CoA carboxylase, wherein an acyl-CoA synthetase in the hosts are functionally delected. The hosts prefereably include the nucleic acid encoding the thioesterase at an intermediate copy number. The hosts are preferably recominantly stable and growth-competent at 37.degree. C. Methods of producing a fatty acid product comprising culturing such hosts at 37.degree. C. are also described.

Inventors:: Pfleger, Brian F; Lennen, Rebecca M

Issue Date:: 2013-12-31

Research Org.:: Wisconsin Alumni Research Foundation, Madison, WI, USA

Sponsoring Org.:: USDOE

OSTI Identifier:: 1113235

Patent Number(s):: 8617856

Application Number:: 12/984,343

Assignee:: Wisconsin Alumni Research Foundation (Madison, WI)

Patent Classifications (CPCs):: C - CHEMISTRY C12 - BIOCHEMISTRY C12N - MICROORGANISMS OR ENZYMES

C - CHEMISTRY C12 - BIOCHEMISTRY C12P - FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE {

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C - CHEMISTRY C12 - BIOCHEMISTRY C12N - MICROORGANISMS OR ENZYMES
C12N15/8247 - {involving modified lipid metabolism, e.g. seed oil composition}
C12N9/16 - acting on ester bonds
C12N9/93 - {Ligases

C - CHEMISTRY C12 - BIOCHEMISTRY C12P - FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE {
C12P7/6409 - {Fatty acids}

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DOE Contract Number:: FC02-07ER64494; FG02-03ER15468

Resource Type:: Patent

Country of Publication:: United States

Language:: English

Subject:: 59 BASIC BIOLOGICAL SCIENCES

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                    Pfleger, Brian F, and Lennen, Rebecca M. Fatty acid-producing hosts.  United States: N. p., 2013. 
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                    Pfleger, Brian F, & Lennen, Rebecca M. Fatty acid-producing hosts.  United States.

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                    Pfleger, Brian F, and Lennen, Rebecca M. Tue .  
        "Fatty acid-producing hosts".  United States.  https://www.osti.gov/servlets/purl/1113235.

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@article{osti_1113235,

  title        = {Fatty acid-producing hosts},

  author       = {Pfleger, Brian F and Lennen, Rebecca M},

  abstractNote = {Described are hosts for overproducing a fatty acid product such as a fatty acid. The hosts include an exogenous nucleic acid encoding a thioesterase and, optionally, an exogenous nucleic acid encoding an acetyl-CoA carboxylase, wherein an acyl-CoA synthetase in the hosts are functionally delected. The hosts prefereably include the nucleic acid encoding the thioesterase at an intermediate copy number. The hosts are preferably recominantly stable and growth-competent at 37.degree. C. Methods of producing a fatty acid product comprising culturing such hosts at 37.degree. C. are also described.},

  doi          = {},

  journal      = {},
number       = ,

  volume       = ,

  place        = {United States},

  year         = {2013},

  month        = {12}

}

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This present invention provides for a genetically modified host cell, or a cell-free reaction system, and related methods and materials for the biocatalytic production of an alkyl lactones from a hydroxy fatty acid, or natural alkyl lactones and esters from sugars using non-natural combinations of enzymes.
Full Text Available
Methods of refining and producing isomerized fatty acid esters and fatty acids from natural oil feedstocks

Patent Snead, Thomas E.; Cohen, Steven A.; Gildon, Demond L.; ...

Methods are provided for refining natural oil feedstocks and producing isomerized esters and acids. The methods comprise providing a C4-C18 unsaturated fatty ester or acid, and isomerizing the fatty acid ester or acid in the presence of heat or an isomerization catalyst to form an isomerized fatty ester or acid. In some embodiments, the methods comprise forming a dibasic ester or dibasic acid prior to the isomerizing step. In certain embodiments, the methods further comprise hydrolyzing the dibasic ester to form a dibasic acid. In certain embodiments, the olefin is formed by reacting the feedstock in the presence of amore » « less
Full Text Available
Yeast host cells and methods for producing fatty alcohols

Patent D'Espaux, Leopold; Keasling, Jay D.

The present invention provides for a genetically modified yeast cell comprising at least six or more of the following modifications: increased expression of Mus musculus fatty acid reductase, acetyl-CoA carboxylase, fatty acid synthase 1, fatty acid synthase 2, a mutant of the bottleneck enzyme encoded by ACC1 insensitive to post-transcriptional and post-translational repression, and/or a desaturase encoded by OLE1, and reduced expression of DGA1, HFD1, ADH6, and/or GDH1. The present invention provides a method for constructing the genetically modified yeast cell, and a method for producing a fatty alcohol from the genetically modified yeast cell.
Full Text Available
Use of plant fatty acyl hydroxylases to produce hydroxylated fatty acids and derivatives in plants

Patent Somerville, Chris [Portola Valley, CA]; van de Loo, Frank [Lexington, KY]

The present invention relates to the identification of nucleic acid sequences and constructs, and methods related thereto, and the use of these sequences and constructs to produce genetically modified plants for the purpose of altering the composition of plant oils, waxes and related compounds.
Full Text Available
Use of plant fatty acyl hydroxylases to produce hydroxylated fatty acids and derivatives in plants

Patent Somerville, Chris [Portola Valley, CA]; van de Loo, Frank [Lexington, KY]

The present invention relates to the identification of nucleic acid sequences and constructs, and methods related thereto, and the use of these sequences and constructs to produce genetically modified plants for the purpose of altering the composition of plant oils, waxes and related compounds.
Full Text Available

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Title: Fatty acid-producing hosts

Abstract

Citation Formats

Inhibition of Escherichia coli Acetyl Coenzyme A Carboxylase by Acyl-Acyl Carrier Protein journal, February 2001

Construction and characterization of amplifiable multicopy DNA cloning vehicles derived from the P15A cryptic miniplasmid. journal, January 1978

Copy‐number control of the Escherichia coli chromosome: a plasmidologist's view journal, May 2006

Regulation of Fatty Acid Elongation and Initiation by Acyl-Acyl Carrier Protein in Escherichia coli journal, January 1996

Tuning genetic control through promoter engineering journal, August 2005

Use of baculovirus early promoters for expression of foreign genes in stably transformed insect cells patent, December 1991

Factors affecting the expression of foreign proteins inEscherichia coli journal, February 1987

Broad host range vectors for stable genomic library construction journal, January 2006

E. coli Genome Manipulation by P1 Transduction journal, May 2014

Transformation of Kluyveromyces fragilis journal, June 1984

Alteration of Acyl-Acyl Carrier Protein Pools and Acetyl-CoA Carboxylase Expression in Escherichia coli by a Plant Medium-Chain Acyl-Acyl Carrier Protein Thioesterase journal, February 1995

Growth rate regulation of Escherichia coli acetyl coenzyme A carboxylase, which catalyzes the first committed step of lipid biosynthesis. journal, January 1993

High-level production of amorpha-4,11-diene in a two-phase partitioning bioreactor of metabolically engineeredEscherichia coli journal, January 2006

A Role for the umuDC Gene Products of Escherichia coli in Increasing Resistance to DNA Damage in Stationary Phase by Inhibiting the Transition to Exponential Growth journal, February 2000

Influence of glucose and other substrates on electric field and polyethylene glycol-mediated transformation of intact yeast cells journal, August 1994

In Vivo Functional Analyses of the Type II Acyl Carrier Proteins of Fatty Acid Biosynthesis journal, May 2007

Solubility of long-chain fatty acids in phosphate buffer at pH 7.4 journal, June 1992

Transmembrane Movement of Exogenous Long-Chain Fatty Acids: Proteins, Enzymes, and Vectorial Esterification journal, September 2003

High Efficiency transformation of Saccharomyces cerevisiae by electroporation journal, January 1993

Transformation of yeast. journal, April 1978

Microdiesel: Escherichia coli engineered for fuel production journal, September 2006

Amino terminus of the yeast GAL4 gene product is sufficient for nuclear localization. journal, October 1984

Overproduction of free fatty acids in E. coli: Implications for biodiesel production journal, November 2008

Functionally homologous cell cycle control genes in budding and fission yeast journal, December 1982

Isolation of the yeast regulatory gene GAL4 and analysis of its dosage effects on the galactose/melibiose regulon. journal, November 1982

Transformation of the industrially important yeastsCandida maltosa andPichia guilliermondii journal, January 1985

Low-Copy Plasmids can Perform as Well as or Better Than High-Copy Plasmids for Metabolic Engineering of Bacteria journal, October 2000

Microbial Biosynthesis of Alkanes journal, July 2010

Minimal essential origin of plasmid pSC101 replication: requirement of a region downstream of iterons journal, September 1993

A Rapid Method of Total Lipid Extraction and Purification journal, August 1959

A family of arabinose-inducible Escherichia coli expression vectors having pBR322 copy control journal, March 2006

Fate of transforming DNA following uptake by competent Bacillus subtilis: I. Formation and properties of the donor-recipient complex journal, March 1971

Characterization and cloning of a stearoyl/oleoyl specific fatty acyl-acyl carrier protein thioesterase from the seeds of Madhuca longifolia (latifolia) journal, December 2007

Expression of Lauroyl–Acyl Carrier Protein Thioesterase in Brassica napus Seeds Induces Pathways for Both Fatty Acid Oxidation and Biosynthesis and Implies a Set Point for Triacylglycerol Accumulation journal, April 1998

Pichia pastoris as a host system for transformations journal, December 1985

Ethanol production by recombinant hosts patent, June 1995

Quorum sensing: the many languages of bacteria journal, January 2006

Overproduction of Acetyl-CoA Carboxylase Activity Increases the Rate of Fatty Acid Biosynthesis in Escherichia coli journal, July 2000

Escherichia coli Unsaturated Fatty Acid Synthesis: COMPLEX TRANSCRIPTION OF THE fabA GENE AND IN VIVO IDENTIFICATION OF THE ESSENTIAL REACTION CATALYZED BY FabB journal, August 2009

Transformation of the Methylotrophic Yeast Hansenula polymorpha journal, December 1986

In vivo sequence requirements of the SV40 early promoter region journal, March 1981

Transformation of the methylotrophic yeast Hansenula polymorpha by autonomous replication and integration vectors journal, February 1986

Transformation of Kluyveromyces lactis by killer plasmid DNA journal, May 1983

Use of a two-phase partitioning bioreactor for degrading polycyclic aromatic hydrocarbons by a Sphingomonas sp. journal, July 2002

Integrative transformation of Candida albicans, using a cloned Candida ADE2 gene. journal, January 1986

Cloning, characterization and structural model of a FatA-type thioesterase from sunflower seeds (Helianthus annuus L.) journal, April 2005

Fatty acid biosynthesis redirected to medium chains in transgenic oilseed plants journal, July 1992

Functional relationships between transcriptional control signals of the thymidine kinase gene of herpes simplex virus journal, December 1982

[33] Expression and secretion vectors for yeast book, January 1987

Catalytic Deoxygenation of Fatty Acids and Their Derivatives journal, January 2007

Cloning immunoglobulin variable domains for expression by the polymerase chain reaction. journal, May 1989

Isolation and molecular characterization of a novel broad-host-range plasmid from Bordetella bronchiseptica with sequence similarities to plasmids from Gram-positive organisms journal, July 1992

Recombinant glucose uptake system patent, February 1997

Kinetic modeling analysis of maleic acid-catalyzed hemicellulose hydrolysis in corn stover journal, December 2008

Continuous decarboxylation of lauric acid over Pd/C catalyst journal, December 2008

Autonomous replication sequences for yeast strains of the genus pichia patent, June 1989

Powerful and versatile enhancer-promoter unit for mammalian expression vectors journal, January 1986

Improved M13 phage cloning vectors and host strains: nucleotide sequences of the M13mpl8 and pUC19 vectors journal, January 1985

Ethanol production using engineered mutant E. coli patent, July 1991

Ethanol production by Escherichia coli strains co-expressing Zymomonas PDC and ADH genes patent, March 1991

DNA sequences, vectors and fusion polypeptides to increase secretion of desired polypeptides from filamentous fungi patent, October 1997

Cloning of the nitrate reductase gene (niaD) of Aspergillus nidulans and its use for transformation of Fusarium oxysporum journal, May 1989

Bacillus subtilis (natto) plasmid pLS20 mediates interspecies plasmid transfer. journal, November 1987

Transmembrane passage of hydrophobic compounds through a protein channel wall journal, February 2009

The copy-number of plasmids and other genetic elements can be determined by SYBR-Green-based quantitative real-time PCR journal, June 2006

PHYSIOLOGICAL AND GENETIC FACTORS AFFECTING TRANSFORMATION OF BACILLUS SUBTILIS journal, May 1961

One-step inactivation of chromosomal genes in Escherichia coli K-12 using PCR products journal, May 2000

Tight regulation, modulation, and high-level expression by vectors containing the arabinose PBAD promoter. journal, July 1995

Pichia transformation patent, May 1990

Multiple transformation of Saccharomyces cerevisiae by protoplast fusion journal, December 1992

Kluyveromyces as a Host for Heterologous Gene Expression: Expression and Secretion of Prochymosin journal, February 1990

Ethanol production in Gram-positive microbes patent, January 1996

High frequency transformation of Bacillus subtilis protoplasts by plasmid DNA journal, January 1979

Cloning and functional expression of an acyl-ACP thioesterase FatB type from Diploknema (Madhuca) butyracea seeds in Escherichia coli journal, November 2006

Regulation of quorum sensing in Pseudomonas journal, March 2006

Tolerance of bacteria to organic solvents journal, June 2002

Bacterial Small-Molecule Signaling Pathways journal, February 2006

Inhibition of Escherichia coli Acetyl Coenzyme A Carboxylase by Acyl-Acyl Carrier Protein
journal, February 2001

Construction and characterization of amplifiable multicopy DNA cloning vehicles derived from the P15A cryptic miniplasmid.
journal, January 1978

Copy‐number control of the Escherichia coli chromosome: a plasmidologist's view
journal, May 2006

Regulation of Fatty Acid Elongation and Initiation by Acyl-Acyl Carrier Protein in Escherichia coli
journal, January 1996

Tuning genetic control through promoter engineering
journal, August 2005

Use of baculovirus early promoters for expression of foreign genes in stably transformed insect cells
patent, December 1991

Factors affecting the expression of foreign proteins inEscherichia coli
journal, February 1987

Broad host range vectors for stable genomic library construction
journal, January 2006

E. coli Genome Manipulation by P1 Transduction
journal, May 2014

Transformation of Kluyveromyces fragilis
journal, June 1984

Alteration of Acyl-Acyl Carrier Protein Pools and Acetyl-CoA Carboxylase Expression in Escherichia coli by a Plant Medium-Chain Acyl-Acyl Carrier Protein Thioesterase
journal, February 1995

Growth rate regulation of Escherichia coli acetyl coenzyme A carboxylase, which catalyzes the first committed step of lipid biosynthesis.
journal, January 1993

High-level production of amorpha-4,11-diene in a two-phase partitioning bioreactor of metabolically engineeredEscherichia coli
journal, January 2006

A Role for the umuDC Gene Products of Escherichia coli in Increasing Resistance to DNA Damage in Stationary Phase by Inhibiting the Transition to Exponential Growth
journal, February 2000

Influence of glucose and other substrates on electric field and polyethylene glycol-mediated transformation of intact yeast cells
journal, August 1994

In Vivo Functional Analyses of the Type II Acyl Carrier Proteins of Fatty Acid Biosynthesis
journal, May 2007

Solubility of long-chain fatty acids in phosphate buffer at pH 7.4
journal, June 1992

Transmembrane Movement of Exogenous Long-Chain Fatty Acids: Proteins, Enzymes, and Vectorial Esterification
journal, September 2003

High Efficiency transformation of Saccharomyces cerevisiae by electroporation
journal, January 1993

Transformation of yeast.
journal, April 1978

Microdiesel: Escherichia coli engineered for fuel production
journal, September 2006

Amino terminus of the yeast GAL4 gene product is sufficient for nuclear localization.
journal, October 1984

Overproduction of free fatty acids in E. coli: Implications for biodiesel production
journal, November 2008

Functionally homologous cell cycle control genes in budding and fission yeast
journal, December 1982

Isolation of the yeast regulatory gene GAL4 and analysis of its dosage effects on the galactose/melibiose regulon.
journal, November 1982

Transformation of the industrially important yeastsCandida maltosa andPichia guilliermondii
journal, January 1985

Low-Copy Plasmids can Perform as Well as or Better Than High-Copy Plasmids for Metabolic Engineering of Bacteria
journal, October 2000

Microbial Biosynthesis of Alkanes
journal, July 2010

Minimal essential origin of plasmid pSC101 replication: requirement of a region downstream of iterons
journal, September 1993

A Rapid Method of Total Lipid Extraction and Purification
journal, August 1959

A family of arabinose-inducible Escherichia coli expression vectors having pBR322 copy control
journal, March 2006

Fate of transforming DNA following uptake by competent Bacillus subtilis: I. Formation and properties of the donor-recipient complex
journal, March 1971

Characterization and cloning of a stearoyl/oleoyl specific fatty acyl-acyl carrier protein thioesterase from the seeds of Madhuca longifolia (latifolia)
journal, December 2007

Expression of Lauroyl–Acyl Carrier Protein Thioesterase in Brassica napus Seeds Induces Pathways for Both Fatty Acid Oxidation and Biosynthesis and Implies a Set Point for Triacylglycerol Accumulation
journal, April 1998

Pichia pastoris as a host system for transformations
journal, December 1985

Ethanol production by recombinant hosts
patent, June 1995

Quorum sensing: the many languages of bacteria
journal, January 2006

Overproduction of Acetyl-CoA Carboxylase Activity Increases the Rate of Fatty Acid Biosynthesis in Escherichia coli
journal, July 2000

Escherichia coli Unsaturated Fatty Acid Synthesis: COMPLEX TRANSCRIPTION OF THE fabA GENE AND IN VIVO IDENTIFICATION OF THE ESSENTIAL REACTION CATALYZED BY FabB
journal, August 2009

Transformation of the Methylotrophic Yeast Hansenula polymorpha
journal, December 1986

In vivo sequence requirements of the SV40 early promoter region
journal, March 1981

Transformation of the methylotrophic yeast Hansenula polymorpha by autonomous replication and integration vectors
journal, February 1986

Transformation of Kluyveromyces lactis by killer plasmid DNA
journal, May 1983

Use of a two-phase partitioning bioreactor for degrading polycyclic aromatic hydrocarbons by a Sphingomonas sp.
journal, July 2002

Integrative transformation of Candida albicans, using a cloned Candida ADE2 gene.
journal, January 1986

Cloning, characterization and structural model of a FatA-type thioesterase from sunflower seeds (Helianthus annuus L.)
journal, April 2005

Fatty acid biosynthesis redirected to medium chains in transgenic oilseed plants
journal, July 1992

Functional relationships between transcriptional control signals of the thymidine kinase gene of herpes simplex virus
journal, December 1982

[33] Expression and secretion vectors for yeast
book, January 1987

Catalytic Deoxygenation of Fatty Acids and Their Derivatives
journal, January 2007

Cloning immunoglobulin variable domains for expression by the polymerase chain reaction.
journal, May 1989

Isolation and molecular characterization of a novel broad-host-range plasmid from Bordetella bronchiseptica with sequence similarities to plasmids from Gram-positive organisms
journal, July 1992

Recombinant glucose uptake system
patent, February 1997

Kinetic modeling analysis of maleic acid-catalyzed hemicellulose hydrolysis in corn stover
journal, December 2008

Continuous decarboxylation of lauric acid over Pd/C catalyst
journal, December 2008

Autonomous replication sequences for yeast strains of the genus pichia
patent, June 1989

Powerful and versatile enhancer-promoter unit for mammalian expression vectors
journal, January 1986

Improved M13 phage cloning vectors and host strains: nucleotide sequences of the M13mpl8 and pUC19 vectors
journal, January 1985

Ethanol production using engineered mutant E. coli
patent, July 1991

Ethanol production by Escherichia coli strains co-expressing Zymomonas PDC and ADH genes
patent, March 1991

DNA sequences, vectors and fusion polypeptides to increase secretion of desired polypeptides from filamentous fungi
patent, October 1997

Cloning of the nitrate reductase gene (niaD) of Aspergillus nidulans and its use for transformation of Fusarium oxysporum
journal, May 1989

Bacillus subtilis (natto) plasmid pLS20 mediates interspecies plasmid transfer.
journal, November 1987

Transmembrane passage of hydrophobic compounds through a protein channel wall
journal, February 2009

The copy-number of plasmids and other genetic elements can be determined by SYBR-Green-based quantitative real-time PCR
journal, June 2006

PHYSIOLOGICAL AND GENETIC FACTORS AFFECTING TRANSFORMATION OF BACILLUS SUBTILIS
journal, May 1961

One-step inactivation of chromosomal genes in Escherichia coli K-12 using PCR products
journal, May 2000

Tight regulation, modulation, and high-level expression by vectors containing the arabinose PBAD promoter.
journal, July 1995

Pichia transformation
patent, May 1990

Multiple transformation of Saccharomyces cerevisiae by protoplast fusion
journal, December 1992

Kluyveromyces as a Host for Heterologous Gene Expression: Expression and Secretion of Prochymosin
journal, February 1990

Ethanol production in Gram-positive microbes
patent, January 1996

High frequency transformation of Bacillus subtilis protoplasts by plasmid DNA
journal, January 1979

Cloning and functional expression of an acyl-ACP thioesterase FatB type from Diploknema (Madhuca) butyracea seeds in Escherichia coli
journal, November 2006

Regulation of quorum sensing in Pseudomonas
journal, March 2006

Tolerance of bacteria to organic solvents
journal, June 2002

Bacterial Small-Molecule Signaling Pathways
journal, February 2006

Integrative transformation of the yeast Yarrowia lipolytica
journal, October 1985

Modification of the substrate specificity of an acyl-acyl carrier protein thioesterase by protein engineering.
journal, November 1995

Fatty Acid Degradation in Escherichia coli: An Inducible Acyl-CoA Synthetase, the Mapping of old-Mutations, and the Isolation of Regulatory Mutants
journal, February 1969