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Title: Production of carrier-peptide conjugates using chemically reactive unnatural amino acids

Abstract

Provided are methods of making carrier polypeptide that include incorporating a first unnatural amino acid into a carrier polypeptide variant, incorporating a second unnatural amino acid into a target polypeptide variant, and reacting the first and second unnatural amino acids to produce the conjugate. Conjugates produced using the provided methods are also provided. In addition, orthogonal translation systems in methylotrophic yeast and methods of using these systems to produce carrier and target polypeptide variants comprising unnatural amino acids are provided.

Inventors:
;
Issue Date:
Research Org.:
The Scripps Research Institute, La Jolla, CA, USA
Sponsoring Org.:
USDOE
OSTI Identifier:
1111109
Patent Number(s):
8609383
Application Number:
13/133,629
Assignee:
The Scripps Research Institute (La Jolla, CA)
Patent Classifications (CPCs):
C - CHEMISTRY C07 - ORGANIC CHEMISTRY C07K - PEPTIDES
C - CHEMISTRY C12 - BIOCHEMISTRY C12N - MICROORGANISMS OR ENZYMES
DOE Contract Number:  
FG03-00ER46051
Resource Type:
Patent
Country of Publication:
United States
Language:
English
Subject:
37 INORGANIC, ORGANIC, PHYSICAL, AND ANALYTICAL CHEMISTRY

Citation Formats

Young, Travis, and Schultz, Peter G. Production of carrier-peptide conjugates using chemically reactive unnatural amino acids. United States: N. p., 2013. Web.
Young, Travis, & Schultz, Peter G. Production of carrier-peptide conjugates using chemically reactive unnatural amino acids. United States.
Young, Travis, and Schultz, Peter G. Tue . "Production of carrier-peptide conjugates using chemically reactive unnatural amino acids". United States. https://www.osti.gov/servlets/purl/1111109.
@article{osti_1111109,
title = {Production of carrier-peptide conjugates using chemically reactive unnatural amino acids},
author = {Young, Travis and Schultz, Peter G},
abstractNote = {Provided are methods of making carrier polypeptide that include incorporating a first unnatural amino acid into a carrier polypeptide variant, incorporating a second unnatural amino acid into a target polypeptide variant, and reacting the first and second unnatural amino acids to produce the conjugate. Conjugates produced using the provided methods are also provided. In addition, orthogonal translation systems in methylotrophic yeast and methods of using these systems to produce carrier and target polypeptide variants comprising unnatural amino acids are provided.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {2013},
month = {12}
}

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