DOE Patents title logo U.S. Department of Energy
Office of Scientific and Technical Information

Title: Method of detecting genetic deletions identified with chromosomal abnormalities

Abstract

Methods and compositions for staining based upon nucleic acid sequence that employ nucleic acid probes are provided. Said methods produce staining patterns that can be tailored for specific cytogenetic analyzes. Said probes are appropriate for in situ hybridization and stain both interphase and metaphase chromosomal material with reliable signals. The nucleic acids probes are typically of a complexity greater tha 50 kb, the complexity depending upon the cytogenetic application. Methods and reagents are provided for the detection of genetic rearrangements. Probes and test kits are provided for use in detecting genetic rearrangements, particlularly for use in tumor cytogenetics, in the detection of disease related loci, specifically cancer, such as chronic myelogenous leukemia (CML) and for biological dosimetry. Methods and reagents are described for cytogenetic research, for the differentiation of cytogenetically similar ut genetically different diseases, and for many prognostic and diagnostic applications.

Inventors:
; ;
Issue Date:
Research Org.:
Lawrence Berkeley National Lab. (LBNL), Berkeley, CA (United States)
Sponsoring Org.:
USDOE
OSTI Identifier:
1109099
Patent Number(s):
8592155
Application Number:
10/608,092
Assignee:
The Regents of the University of California (Oakland, CA)
Patent Classifications (CPCs):
C - CHEMISTRY C12 - BIOCHEMISTRY C12Q - MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS
DOE Contract Number:  
W-7405-ENG-48
Resource Type:
Patent
Country of Publication:
United States
Language:
English
Subject:
60 APPLIED LIFE SCIENCES

Citation Formats

Gray, Joe W, Pinkel, Daniel, and Tkachuk, Douglas. Method of detecting genetic deletions identified with chromosomal abnormalities. United States: N. p., 2013. Web.
Gray, Joe W, Pinkel, Daniel, & Tkachuk, Douglas. Method of detecting genetic deletions identified with chromosomal abnormalities. United States.
Gray, Joe W, Pinkel, Daniel, and Tkachuk, Douglas. Tue . "Method of detecting genetic deletions identified with chromosomal abnormalities". United States. https://www.osti.gov/servlets/purl/1109099.
@article{osti_1109099,
title = {Method of detecting genetic deletions identified with chromosomal abnormalities},
author = {Gray, Joe W and Pinkel, Daniel and Tkachuk, Douglas},
abstractNote = {Methods and compositions for staining based upon nucleic acid sequence that employ nucleic acid probes are provided. Said methods produce staining patterns that can be tailored for specific cytogenetic analyzes. Said probes are appropriate for in situ hybridization and stain both interphase and metaphase chromosomal material with reliable signals. The nucleic acids probes are typically of a complexity greater tha 50 kb, the complexity depending upon the cytogenetic application. Methods and reagents are provided for the detection of genetic rearrangements. Probes and test kits are provided for use in detecting genetic rearrangements, particlularly for use in tumor cytogenetics, in the detection of disease related loci, specifically cancer, such as chronic myelogenous leukemia (CML) and for biological dosimetry. Methods and reagents are described for cytogenetic research, for the differentiation of cytogenetically similar ut genetically different diseases, and for many prognostic and diagnostic applications.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {2013},
month = {11}
}

Works referenced in this record:

Altered Transcription of an Oncogene in Chronic Myeloid Leukaemia
journal, March 1984


Localization of the c-abl oncogene adjacent to a translocation break point in chronic myelocytic leukaemia
journal, November 1983


bcr-abl RNA in patients with chronic myelogenous leukemia
journal, March 1987


Rearrangement and amplification of c-abl sequences in the human chronic myelogenous leukemia cell line K-562.
journal, August 1983


Detection of the Philadelphia chromosome in interphase nuclei
journal, January 1990


Fluorescence in situ hybridization with human chromosome-specific libraries: detection of trisomy 21 and translocations of chromosome 4.
journal, December 1988


The Chronic Myelogenous Leukemia-Specific P210 Protein Is the Product of the bcr / abl Hybrid Gene
journal, July 1986


Diagnosis of chronic myeloid and acute lymphocytic leukemias by detection of leukemia-specific mRNA sequences amplified in vitro.
journal, August 1988


Modified nucleotides and methods of preparing and using same
patent, December 1987


Translocation of c-abl oncogene correlates with the presence of a Philadelphia chromosome in chronic myelocytic leukaemia
journal, November 1983


The chronic myelocytic cell line K562 contains a breakpoint in bcr and produces a chimeric bcr/c-abl transcript
journal, February 1986


Undetectablebcr-abl rearrangements in some CML patients are due to a deletion mutation in thebcr gene
journal, May 1988


Philadelphia chromosomal breakpoints are clustered within a limited region, bcr, on chromosome 22
journal, January 1984


Cell lines and clinical isolates derived from Ph1-positive chronic myelogenous leukemia patients express c-abl proteins with a common structural alteration.
journal, March 1985


A cellular oncogene is translocated to the Philadelphia chromosome in chronic myelocytic leukaemia
journal, December 1982


Chemically cleavable nucleotides
patent, September 1988


Bi-color detection of two target DNAs by non-radioactive in situ hybridization
journal, January 1986


Utility of molecular genetic analysis of bcr rearrangement in the diagnosis of chronic myeloid leukemia
journal, November 1987


Cytogenetic analysis using quantitative, high-sensitivity, fluorescence hybridization.
journal, May 1986


Persistence of chronic myelocytic leukemia despite deletion of rearranged bcr/c-abl sequences in blast crisis.
journal, November 1986


A simple method of reducing the fading of immunofluorescence during microscopy
journal, June 1981


Evidence for the Involvement of GM-CSF and FMS in the Deletion (5q) in Myeloid Disorders
journal, February 1986


Use of whole cosmid cloned genomic sequences for chromosomal localization by non-radioactive in situ hybridization
journal, December 1987


Unique fusion of bcr and c-abl genes in Philadelphia chromosome positive acute lymphoblastic leukemia
journal, October 1987


An 8-kilobase abl RNA transcript in chronic myelogenous leukemia.
journal, September 1984


The first BCR gene intron contains breakpoints in Philadelphla chromosome positive leukemia
journal, January 1988


Expression of a distinctive BCR-ABL oncogene in Ph1-positive acute lymphocytic leukemia (ALL)
journal, February 1988


Process for amplifying, detecting, and/or-cloning nucleic acid sequences
patent, July 1987


Molecular detection of deletions involving band q14 of chromosome 13 in retinoblastomas.
journal, October 1986


Long-range mapping of the Philadelphia chromosome by pulsed-field gel electrophoresis
journal, March 1988


Alternative splicing of RNAs transcribed from the human abl gene and from the bcr-abl fused gene
journal, October 1986


A new fused transcript in Philadelphia chromosome positive acute lymphocytic leukaemia
journal, November 1987


Methods for chromosome-specific staining
patent, September 1995


Structural organization of the bcr gene and its role in the Ph′ translocation
journal, June 1985


Cytogenetic and molecular analysis in Philadelphia negative CML
journal, March 1989


Multiple fusion probes
patent, July 2002