Lignin blockers and uses thereof

Yang, Bin; Wyman, Charles E

Advanced Search OptionsAdvanced Search queries use a traditional Term Search. For more info, see our FAQ.

All Fields:

Patent Title:

Abstract:

Assignee:

Inventor(s):

Patent Number:

Patent Classification (CPC):

All Classifications
A - human necessities
A01 - agriculture
A21 - baking
A22 - butchering
A23 - foods or foodstuffs
A24 - tobacco
A41 - wearing apparel
A42 - headwear
A43 - footwear
A44 - haberdashery
A45 - hand or travelling articles
A46 - brushware
A47 - furniture
A61 - medical or veterinary science
A62 - life-saving
A63 - sports
A99 - subject matter not otherwise provided for in this section
B - performing operations
B01 - physical or chemical processes or apparatus in general
B02 - crushing, pulverising, or disintegrating
B03 - separation of solid materials using liquids or using pneumatic tables or jigs
B04 - centrifugal apparatus or machines for carrying-out physical or chemical processes
B05 - spraying or atomising in general
B06 - generating or transmitting mechanical vibrations in general
B07 - separating solids from solids
B08 - cleaning
B09 - disposal of solid waste
B21 - mechanical metal-working without essentially removing material
B22 - casting
B23 - machine tools
B24 - grinding
B25 - hand tools
B26 - hand cutting tools
B27 - working or preserving wood or similar material
B28 - working cement, clay, or stone
B29 - working of plastics
B30 - presses
B31 - making articles of paper, cardboard or material worked in a manner analogous to paper
B32 - layered products
B33 - additive manufacturing technology
B41 - printing
B42 - bookbinding
B43 - writing or drawing implements
B44 - decorative arts
B60 - vehicles in general
B61 - railways
B62 - land vehicles for travelling otherwise than on rails
B63 - ships or other waterborne vessels
B64 - aircraft
B65 - conveying
B66 - hoisting
B67 - opening, closing {or cleaning} bottles, jars or similar containers
B68 - saddlery
B81 - microstructural technology
B82 - nanotechnology
B99 - subject matter not otherwise provided for in this section
C - chemistry
C01 - inorganic chemistry
C02 - treatment of water, waste water, sewage, or sludge
C03 - glass
C04 - cements
C05 - fertilisers
C06 - explosives
C07 - organic chemistry
C08 - organic macromolecular compounds
C09 - dyes
C10 - petroleum, gas or coke industries
C11 - animal or vegetable oils, fats, fatty substances or waxes
C12 - biochemistry
C13 - sugar industry
C14 - skins
C21 - metallurgy of iron
C22 - metallurgy
C23 - coating metallic material
C25 - electrolytic or electrophoretic processes
C30 - crystal growth
C40 - combinatorial technology
C99 - subject matter not otherwise provided for in this section
D - textiles
D01 - natural or man-made threads or fibres
D02 - yarns
D03 - weaving
D04 - braiding
D05 - sewing
D06 - treatment of textiles or the like
D07 - ropes
D10 - indexing scheme associated with sublasses of section d, relating to textiles
D21 - paper-making
D99 - subject matter not otherwise provided for in this section
E - fixed constructions
E01 - construction of roads, railways, or bridges
E02 - hydraulic engineering
E03 - water supply
E04 - building
E05 - locks
E06 - doors, windows, shutters, or roller blinds in general
E21 - earth drilling
E99 - subject matter not otherwise provided for in this section
F - mechanical engineering
F01 - machines or engines in general
F02 - combustion engines
F03 - machines or engines for liquids
F04 - positive - displacement machines for liquids
F05 - indexing schemes relating to engines or pumps in various subclasses of classes f01-f04
F15 - fluid-pressure actuators
F16 - engineering elements and units
F17 - storing or distributing gases or liquids
F21 - lighting
F22 - steam generation
F23 - combustion apparatus
F24 - heating
F25 - refrigeration or cooling
F26 - drying
F27 - furnaces
F28 - heat exchange in general
F41 - weapons
F42 - ammunition
F99 - subject matter not otherwise provided for in this section
G - physics
G01 - measuring
G02 - optics
G03 - photography
G04 - horology
G05 - controlling
G06 - computing
G07 - checking-devices
G08 - signalling
G09 - education
G10 - musical instruments
G11 - information storage
G12 - instrument details
G16 - information and communication technology [ict] specially adapted for specific application fields
G21 - nuclear physics
G99 - subject matter not otherwise provided for in this section
H - electricity
H01 - basic electric elements
H02 - generation
H03 - basic electronic circuitry
H04 - electric communication technique
H05 - electric techniques not otherwise provided for
H99 - subject matter not otherwise provided for in this section
Y - new / cross sectional technologies
Y02 - technologies or applications for mitigation or adaptation against climate change
Y04 - information or communication technologies having an impact on other technology areas
Y10 - technical subjects covered by former uspc

More Options ...

Title: Lignin blockers and uses thereof

Abstract

Disclosed is a method for converting cellulose in a lignocellulosic biomass. The method provides for a lignin-blocking polypeptide and/or protein treatment of high lignin solids. The treatment enhances cellulase availability in cellulose conversion and allows for the determination of optimized pretreatment conditions. Additionally, ethanol yields from a Simultaneous Saccharification and Fermentation process are improved 5-25% by treatment with a lignin-blocking polypeptide and/or protein.

Inventors:: Yang, Bin; Wyman, Charles E

Issue Date:: 2013-11-12

Research Org.:: Dartmouth College (Hanover, NH)

Sponsoring Org.:: USDOE

OSTI Identifier:: 1107920

Patent Number(s):: 8580541

Application Number:: 12/962,366

Assignee:: The Trustees of Dartmouth College (Hanover, NH)

Patent Classifications (CPCs):: C - CHEMISTRY C12 - BIOCHEMISTRY C12P - FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE {

Y - NEW / CROSS SECTIONAL TECHNOLOGIES Y02 - TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE Y02E - REDUCTION OF GREENHOUSE GAS [GHG] EMISSIONS, RELATED TO ENERGY GENERATION, TRANSMISSION OR DISTRIBUTION

Show more

C - CHEMISTRY C12 - BIOCHEMISTRY C12P - FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE {
C12P19/02 - Monosaccharides
C12P7/08 - produced as by-product or from waste or cellulosic material substrate
C12P7/10 - substrate containing cellulosic material

Y - NEW / CROSS SECTIONAL TECHNOLOGIES Y02 - TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE Y02E - REDUCTION OF GREENHOUSE GAS [GHG] EMISSIONS, RELATED TO ENERGY GENERATION, TRANSMISSION OR DISTRIBUTION
Y02E50/10 - Biofuels

Show less

DOE Contract Number:: FC36-00G0010589; FC36-01G011075

Resource Type:: Patent

Country of Publication:: United States

Language:: English

Subject:: 59 BASIC BIOLOGICAL SCIENCES

Citation Formats


                    Yang, Bin, and Wyman, Charles E. Lignin blockers and uses thereof.  United States: N. p., 2013. 
        Web.

Copy to clipboard


                    Yang, Bin, & Wyman, Charles E. Lignin blockers and uses thereof.  United States.

Copy to clipboard


                    Yang, Bin, and Wyman, Charles E. Tue .  
        "Lignin blockers and uses thereof".  United States.  https://www.osti.gov/servlets/purl/1107920.

Copy to clipboard


                    
@article{osti_1107920,

  title        = {Lignin blockers and uses thereof},

  author       = {Yang, Bin and Wyman, Charles E},

  abstractNote = {Disclosed is a method for converting cellulose in a lignocellulosic biomass. The method provides for a lignin-blocking polypeptide and/or protein treatment of high lignin solids. The treatment enhances cellulase availability in cellulose conversion and allows for the determination of optimized pretreatment conditions. Additionally, ethanol yields from a Simultaneous Saccharification and Fermentation process are improved 5-25% by treatment with a lignin-blocking polypeptide and/or protein.},

  doi          = {},

  journal      = {},
number       = ,

  volume       = ,

  place        = {United States},

  year         = {2013},

  month        = {11}

}

Copy to clipboard

Patent:

Works referenced in this record:

Evaluation of the cellobiose-fermenting yeastBrettanomyces custersii in the simultaneous saccharification and fermentation of cellulose
journal, May 1992

Spindler, Diane D.; Wyman, Charles E.; Grohmann, Karel
Biotechnology Letters, Vol. 14, Issue 5
https://doi.org/10.1007/BF01021255

Fast and efficient alkaline peroxide treatment to enhance the enzymatic digestibility of steam-exploded softwood substrates
journal, January 2002

Yang, Bin; Boussaid, Abdel; Mansfield, Shawn D.
Biotechnology and Bioengineering, Vol. 77, Issue 6
https://doi.org/10.1002/bit.10159

Investigation of the Cell-Wall Loosening Protein Expansin as a Possible Additive in the Enzymatic Saccharification of Lignocellulosic Biomass
journal, January 2000

Baker, John O.; King, Michele R.; Adney, William S.
Applied Biochemistry and Biotechnology, Vol. 84-86, Issue 1-9
https://doi.org/10.1385/ABAB:84-86:1-9:217

The Reactions of Lignins with High Temperature Hydrogen Peroxide Part 2. The Oxidation of Kraft Lignin
journal, May 1999

Kadla, J. F.; Chang, H. -m.; Jameel, H.
Holzforschung, Vol. 53, Issue 3
https://doi.org/10.1515/HF.1999.047

Adsorption of high-purity endo-1,4-β-glucanases from Trichoderma reesei on components of lignocellulosic materials: Cellulose, lignin, and xylan
journal, August 1988

Chernoglazov, Vladimir M.; Ermolova, Olga V.; Klyosov, Anatole A.
Enzyme and Microbial Technology, Vol. 10, Issue 8, p. 503-507
https://doi.org/10.1016/0141-0229(88)90029-4

Process for making alcohol from cellulosic material using plural ferments
patent, February 1977

Hoge, William H.
US Patent Document 4,009,075
URL: https://image-ppubs.uspto.gov/dirsearch-public/print/downloadPdf/4009075

Continuous fermentation process for the production of metabolities using a moving filter
patent, November 1997

Choi, Cha-Yong; Yang, Young Lyeol
US Patent Document 5,688,674
URL: https://image-ppubs.uspto.gov/dirsearch-public/print/downloadPdf/5688674

Factors affecting lignin degradation in lignocellulose by Phanerochaete chrysosporium
journal, February 1984

Leisola, Matti S. A.; Ulmer, Duane C.; Fiechter, Armin
Archives of Microbiology, Vol. 137, Issue 2
https://doi.org/10.1007/BF00414462

Two-stage dilute acid prehydrolysis of biomass
patent, June 1992

Grohmann, Karel; Torget, Robert W.
US Patent Document 5,125,977
URL: https://image-ppubs.uspto.gov/dirsearch-public/print/downloadPdf/5125977

Fractionation of Lignocellulosics by Steam-Aqueous Pretreatments [and Discussion]
journal, April 1987

Overend, R. P.; Chornet, E.; Gascoigne, J. A.
Philosophical Transactions of the Royal Society A: Mathematical, Physical and Engineering Sciences, Vol. 321, Issue 1561
https://doi.org/10.1098/rsta.1987.0029

The simultaneous saccharification and fermentation of pretreated woody crops to ethanol
journal, March 1991

Spindler, Diane D.; Wyman, Charles E.; Grohmann, Karel
Applied Biochemistry and Biotechnology, Vol. 28-29, Issue 1
https://doi.org/10.1007/BF02922648

Delignification of lignocellulosic materials with peroxymonophosphoric acid
patent, June 1996

Springer, Edward L.
US Patent Document 5,529,663
URL: https://image-ppubs.uspto.gov/dirsearch-public/print/downloadPdf/5529663

Cellulase Adsorption and an Evaluation of Enzyme Recycle During Hydrolysis of Steam-Exploded Softwood Residues
journal, January 2002

Lu, Yanpin; Yang, Bin; Gregg, David
Applied Biochemistry and Biotechnology, Vol. 98-100, Issue 1-9
https://doi.org/10.1385/ABAB:98-100:1-9:641

Mechanism of surfactant effect in enzymatic hydrolysis of lignocellulose
journal, August 2002

Eriksson, Torny; Börjesson, Johan; Tjerneld, Folke
Enzyme and Microbial Technology, Vol. 31, Issue 3
https://doi.org/10.1016/S0141-0229(02)00134-5

Method the hydrolytic splitting of acid treated comminuted crude cellulose with steam
patent, November 1987

Hinger, Klaus-Jurgen
US Patent Document 4,708,746
URL: https://image-ppubs.uspto.gov/dirsearch-public/print/downloadPdf/4708746

High solids simultaneous saccharification and fermentation of pretreated wheat straw to ethanol
journal, May 1992

Mohagheghi, A.; Tucker, M.; Grohmann, K.
Applied Biochemistry and Biotechnology, Vol. 33, Issue 2
https://doi.org/10.1007/BF02950778

Simultaneous saccharification and fermentation (SSF) of lignocellulosics to ethanol under vacuum cycling and step feeding
journal, April 1984

Ghose, T. K.; Roychoudhury, P. K.; Ghosh, P.
Biotechnology and Bioengineering, Vol. 26, Issue 4
https://doi.org/10.1002/bit.260260414

Process for hydrolysis of biomass
patent, December 1985

Converse, Alvin O.; Grethlein, Hans E.
US Patent Document 4,556,430
URL: https://image-ppubs.uspto.gov/dirsearch-public/print/downloadPdf/4556430

Method of countercurrent acid hydrolysis of comminuted cellulosic fibrous material
patent, May 1987

Sherman, Michael I.
US Patent Document 4,668,340
URL: https://image-ppubs.uspto.gov/dirsearch-public/print/downloadPdf/4668340

Compositions and process for colored liquid food or drink
patent, September 1979

Danforth, Richard C.
US Patent Document 4,167,587
URL: https://image-ppubs.uspto.gov/dirsearch-public/print/downloadPdf/4167587

Increasing cellulose accessibility is more important than removing lignin: A comparison of cellulose solvent-based lignocellulose fractionation and soaking in aqueous ammonia
journal, September 2010

Rollin, Joseph A.; Zhu, Zhiguang; Sathitsuksanoh, Noppadon
Biotechnology and Bioengineering, Vol. 108, Issue 1
https://doi.org/10.1002/bit.22919

Dilute-acid pretreatment of two short-rotation herbaceous crops: Scientific Note
journal, March 1992

Torget, R.; Himmel, M.; Grohmann, K.
Applied Biochemistry and Biotechnology, Vol. 34-35, Issue 1
https://doi.org/10.1007/BF02920538

Continuous process for ethanol production from lignocellulosic materials without mechanical agitation
patent, November 1993

Lynd, Lee R.; Hogsett, David A.; Spieles, Gisbert
US Patent Document 5,258,293
URL: https://image-ppubs.uspto.gov/dirsearch-public/print/downloadPdf/5258293

Evaluation of Candida acidothermophilum in ethanol production from lignocellulosic biomass
journal, December 1997

Kadam, K. L.; Schmidt, S. L.
Applied Microbiology and Biotechnology, Vol. 48, Issue 6
https://doi.org/10.1007/s002530051120

Hydrolysis and fractionation of lignocellulosic biomass
patent, February 2000

Torget, Robert W.; Padukone, Nandan; Hatzis, Christos
US Patent Document 6,022,419
URL: https://image-ppubs.uspto.gov/dirsearch-public/print/downloadPdf/6022419

Method of treating biomass material
patent, July 1996

Brink, David L.
US Patent Document 5,536,325
URL: https://image-ppubs.uspto.gov/dirsearch-public/print/downloadPdf/5536325

Bacteria for cellulose digestion
patent, August 1989

Waterbury, John B.; Calloway, Charles B.; Turner, Ruth D.
US Patent Document 4,861,721
URL: https://image-ppubs.uspto.gov/dirsearch-public/print/downloadPdf/4861721

Thermotolerant yeast for simultaneous saccharification and fermentation of cellulose to ethanol
journal, April 1988

Spindler, Diane D.; Wyman, Charles E.; Mohagheghi, Ali
Applied Biochemistry and Biotechnology, Vol. 17, Issue 1-3, p. 279-293
https://doi.org/10.1007/BF02779163

Process for pretreating cellulosic substrates and for producing sugar therefrom
patent, December 1980

Grethlein, Hans E.
US Patent Document 4,237,226
URL: https://image-ppubs.uspto.gov/dirsearch-public/print/downloadPdf/4237226

Lignin Impact on Fiber Degradation. 3. Reversal of Inhibition of Enzymatic Hydrolysis by Chemical Modification of Lignin and by Additives
journal, May 1997

Sewalt, V. J. H.; Glasser, W. G.; Beauchemin, K. A.
Journal of Agricultural and Food Chemistry, Vol. 45, Issue 5
https://doi.org/10.1021/jf9608074

Biomass Ethanol: Technical Progress, Opportunities, and Commercial Challenges
journal, November 1999

Wyman, Charles E.
Annual Review of Energy and the Environment, Vol. 24, Issue 1, p. 189-226
https://doi.org/10.1146/annurev.energy.24.1.189

Ethanol from lignocellulosic biomass: Technology, economics, and opportunities
journal, January 1994

Wyman, Charles E.
Bioresource Technology, Vol. 50, Issue 1, p. 3-15
https://doi.org/10.1016/0960-8524(94)90214-3

Method of bioconversion of industrial or agricultural cellulose containing wastes
patent, December 2000

Lizak, Yuri
US Patent Document 6,159,510
URL: https://image-ppubs.uspto.gov/dirsearch-public/print/downloadPdf/6159510

Simultaneous saccharification and fermentation of several lignocellulosic feedstocks to fuel ethanol
journal, January 1992

Wyman, Charles E.; Spindler, Diane D.; Grohmann, Karel
Biomass and Bioenergy, Vol. 3, Issue 5
https://doi.org/10.1016/0961-9534(92)90001-7

Optimization of Steam Explosion to Enhance Hemicellulose Recovery and Enzymatic Hydrolysis of Cellulose in Softwoods
journal, January 1999

Wu, Michael M.; Chang, Kevin; Gregg, David J.
Applied Biochemistry and Biotechnology, Vol. 77, Issue 1-3
https://doi.org/10.1385/ABAB:77:1-3:47

Prehydrolysis of lignocellulose
patent, June 1995

Torget, Robert W.; Kadam, Kiran; Hsu, Teh-An
US Patent Document 5,424,417
URL: https://image-ppubs.uspto.gov/dirsearch-public/print/downloadPdf/5424417

Prehydrolysis of lignocellulose
patent, January 1998

Torget, Robert W.; Kadam, Kiran; Hsu, Teh-An
US Patent Document 5,705,369
URL: https://image-ppubs.uspto.gov/dirsearch-public/print/downloadPdf/5705369

Twenty Years of Trials, Tribulations, and Research Progress in Bioethanol Technology: Selected Key Events Along the Way
journal, January 2001

Wyman, Charles E.
Applied Biochemistry and Biotechnology, Vol. 91-93, Issue 1-9
https://doi.org/10.1385/ABAB:91-93:1-9:5

Continuous process for making ethanol
patent, November 1998

Lynd, Lee R.; South, Colin R.
US Patent Document 5,837,506
URL: https://image-ppubs.uspto.gov/dirsearch-public/print/downloadPdf/5837506

Weak Lignin-Binding Enzymes: A Novel Approach to Improve Activity of Cellulases for Hydrolysis of Lignocellulosics
journal, January 2005

Berlin, Alex; Gilkes, Neil; Kurabi, Arwa
Applied Biochemistry and Biotechnology, Vol. 121, Issue 1-3
https://doi.org/10.1385/ABAB:121:1-3:0163

Fuel Ethanol Production from Lignocellulose Using Genetically Engineered Bacteria
book, May 1997

Ingram, L. O.; Lai, X.; Moniruzzaman, M.
Fuels and Chemicals from Biomass
https://doi.org/10.1021/bk-1997-0666.ch003

Concentrated sulfuric acid hydrolysis of lignocellulosics
patent, May 2000

Hester, Roger D.; Farina, George E.
US Patent Document 6,063,204
URL: https://image-ppubs.uspto.gov/dirsearch-public/print/downloadPdf/6063204

Evaluation of pretreated herbaceous crops for the simultaneous saccharification and fermentation process
journal, March 1990

Spindler, Diana; Wyman, Charles; Grohmann, Karel
Applied Biochemistry and Biotechnology, Vol. 24-25, Issue 1
https://doi.org/10.1007/BF02920252

Alkaline peroxide delignification of agricultural residues to enhance enzymatic saccharification
journal, January 1984

Gould, J. Michael
Biotechnology and Bioengineering, Vol. 26, Issue 1
https://doi.org/10.1002/bit.260260110

Process for extracting lignin from lignocellulosic material using an aqueous organic solvent and an acid neutralizing agent
patent, May 1988

Roberts, Ronnie S.; Muzzy, John D.; Faass, George S.
US Patent Document 4,746,401
URL: https://image-ppubs.uspto.gov/dirsearch-public/print/downloadPdf/4746401

Prehydrolysis of lignocellulose
patent, April 1996

Torget, Robert W.; Kadam, Kiran; Hsu, Teh-An
US Patent Document 5,503,996
URL: https://image-ppubs.uspto.gov/dirsearch-public/print/downloadPdf/5503996

Simultaneous saccharification and fermentation of pretreated wheat straw to ethanol with selected yeast strains and β-glucosidase supplementation
journal, January 1989

Spindler, Diane D.; Wyman, Charles E.; Grohmann, Karel
Applied Biochemistry and Biotechnology, Vol. 20-21, Issue 1
https://doi.org/10.1007/BF02936507

Method for the recovery of formic acid
patent, June 2001

Rousu, Pasi; Anttila, Juha; Rousu, Esa
US Patent Document 6,252,109
URL: https://image-ppubs.uspto.gov/dirsearch-public/print/downloadPdf/6252109

Similar Records in DOE Patents and OSTI.GOV collections:

Lignin blockers and uses thereof

Patent Yang, Bin [West Lebanon, NH]; Wyman, Charles E [Norwich, VT]

Disclosed is a method for converting cellulose in a lignocellulosic biomass. The method provides for a lignin-blocking polypeptide and/or protein treatment of high lignin solids. The treatment enhances cellulase availability in cellulose conversion and allows for the determination of optimized pretreatment conditions. Additionally, ethanol yields from a Simultaneous Saccharification and Fermentation process are improved 5-25% by treatment with a lignin-blocking polypeptide and/or protein. Thus, a more efficient and economical method of processing lignin containing biomass materials utilizes a polypeptide/protein treatment step that effectively blocks lignin binding of cellulase.
Full Text Available
Methods and systems to produce lignin-modifying enzymes and uses thereof

Patent Khlystov, Nikita A.; Sattely, Elizabeth

Embodiments of the invention are generally directed to lignin-modifying enzymes and systems and methods of their manufacture. In many embodiments, the lignin-modifying enzymes are lignin-degrading enzymes capable of breaking down lignin into component parts that are usable for other purposes. Several embodiments are directed to systems for producing lignin-modifying enzymes in vivo, including in yeast and/or plant species, and certain embodiments are directed to methods of creating these systems, including transfecting the species to produce lignin-modifying enzymes.
Full Text Available
Termite enzymes and uses thereof for in vitro conversion of lignin-containing materials to fermentable products

Patent Scharf, Michael E; Boucias, Drion G; Tartar, Aurelien; ...

The disclosure provides isolated nucleic acid molecules derived from the gut of the termite R flavipes, recombinant nucleic acid molecules comprising a vector and an isolated heterologous nucleic acid molecule operably inserted therein, whereby, when transformed into an appropriate host cell system, the heterologous nucleic acid sequence is expressed as a polypeptide having an activity similar to that when expressed in the gut of the termite R. flavipes. The recombinant nucleic acid molecules can comprise more than one heterologous nucleic acid molecule such that more than one polypeptide may be expressed by the host system. The expressed polypeptides may bemore » « less
Full Text Available
Lignin-blocking treatment of biomass and uses thereof

Patent Yang, Bin [Hanover, NH]; Wyman, Charles E [Norwich, VT]

Disclosed is a method for converting cellulose in a lignocellulosic biomass. The method provides for a lignin-blocking polypeptide and/or protein treatment of high lignin solids. The treatment enhances cellulase availability in cellulose conversion. Cellulase efficiencies are improved by the protein or polypeptide treatment. The treatment may be used in combination with steam explosion and acid prehydrolysis techniques. Hydrolysis yields from lignin containing biomass are enhanced 5-20%, and enzyme utilization is increased from 10% to 50%. Thus, a more efficient and economical method of processing lignin containing biomass materials utilizes a polypeptide/protein treatment step that effectively blocks lignin binding of cellulase.
Full Text Available
Enzymes that cleave non-glycosidic ether bonds between lignins or derivatives thereof and saccharides

Patent Kravit, Nancy G.; Schmidt, Katherine A.

The patent application relates to isolated polypeptides that specifically cleave non-glycosidic ether bonds between lignins or derivatives thereof and saccharides, and to cDNAs encoding the polypeptides. The patent application also relates to nucleic acid constructs, expression vectors and host cells comprising the cDNAs, as well as methods of producing and using the isolated polypeptides for treating pulp and biomass to increase soluble saccharide yield and enrich lignin fractions.
Full Text Available

Similar Records

Title: Lignin blockers and uses thereof

Abstract

Citation Formats

Evaluation of the cellobiose-fermenting yeastBrettanomyces custersii in the simultaneous saccharification and fermentation of cellulose journal, May 1992

Fast and efficient alkaline peroxide treatment to enhance the enzymatic digestibility of steam-exploded softwood substrates journal, January 2002

Investigation of the Cell-Wall Loosening Protein Expansin as a Possible Additive in the Enzymatic Saccharification of Lignocellulosic Biomass journal, January 2000

The Reactions of Lignins with High Temperature Hydrogen Peroxide Part 2. The Oxidation of Kraft Lignin journal, May 1999

Adsorption of high-purity endo-1,4-β-glucanases from Trichoderma reesei on components of lignocellulosic materials: Cellulose, lignin, and xylan journal, August 1988

Process for making alcohol from cellulosic material using plural ferments patent, February 1977

Continuous fermentation process for the production of metabolities using a moving filter patent, November 1997

Factors affecting lignin degradation in lignocellulose by Phanerochaete chrysosporium journal, February 1984

Two-stage dilute acid prehydrolysis of biomass patent, June 1992

Fractionation of Lignocellulosics by Steam-Aqueous Pretreatments [and Discussion] journal, April 1987

The simultaneous saccharification and fermentation of pretreated woody crops to ethanol journal, March 1991

Delignification of lignocellulosic materials with peroxymonophosphoric acid patent, June 1996

Cellulase Adsorption and an Evaluation of Enzyme Recycle During Hydrolysis of Steam-Exploded Softwood Residues journal, January 2002

Mechanism of surfactant effect in enzymatic hydrolysis of lignocellulose journal, August 2002

Method the hydrolytic splitting of acid treated comminuted crude cellulose with steam patent, November 1987

High solids simultaneous saccharification and fermentation of pretreated wheat straw to ethanol journal, May 1992

Simultaneous saccharification and fermentation (SSF) of lignocellulosics to ethanol under vacuum cycling and step feeding journal, April 1984

Process for hydrolysis of biomass patent, December 1985

Method of countercurrent acid hydrolysis of comminuted cellulosic fibrous material patent, May 1987

Compositions and process for colored liquid food or drink patent, September 1979

Increasing cellulose accessibility is more important than removing lignin: A comparison of cellulose solvent-based lignocellulose fractionation and soaking in aqueous ammonia journal, September 2010

Dilute-acid pretreatment of two short-rotation herbaceous crops: Scientific Note journal, March 1992

Continuous process for ethanol production from lignocellulosic materials without mechanical agitation patent, November 1993

Evaluation of Candida acidothermophilum in ethanol production from lignocellulosic biomass journal, December 1997

Hydrolysis and fractionation of lignocellulosic biomass patent, February 2000

Method of treating biomass material patent, July 1996

Bacteria for cellulose digestion patent, August 1989

Thermotolerant yeast for simultaneous saccharification and fermentation of cellulose to ethanol journal, April 1988

Process for pretreating cellulosic substrates and for producing sugar therefrom patent, December 1980

Lignin Impact on Fiber Degradation. 3. Reversal of Inhibition of Enzymatic Hydrolysis by Chemical Modification of Lignin and by Additives journal, May 1997

Biomass Ethanol: Technical Progress, Opportunities, and Commercial Challenges journal, November 1999

Ethanol from lignocellulosic biomass: Technology, economics, and opportunities journal, January 1994

Method of bioconversion of industrial or agricultural cellulose containing wastes patent, December 2000

Simultaneous saccharification and fermentation of several lignocellulosic feedstocks to fuel ethanol journal, January 1992

Optimization of Steam Explosion to Enhance Hemicellulose Recovery and Enzymatic Hydrolysis of Cellulose in Softwoods journal, January 1999

Prehydrolysis of lignocellulose patent, June 1995

Prehydrolysis of lignocellulose patent, January 1998

Twenty Years of Trials, Tribulations, and Research Progress in Bioethanol Technology: Selected Key Events Along the Way journal, January 2001

Continuous process for making ethanol patent, November 1998

Weak Lignin-Binding Enzymes: A Novel Approach to Improve Activity of Cellulases for Hydrolysis of Lignocellulosics journal, January 2005

Fuel Ethanol Production from Lignocellulose Using Genetically Engineered Bacteria book, May 1997

Concentrated sulfuric acid hydrolysis of lignocellulosics patent, May 2000

Evaluation of pretreated herbaceous crops for the simultaneous saccharification and fermentation process journal, March 1990

Alkaline peroxide delignification of agricultural residues to enhance enzymatic saccharification journal, January 1984

Process for extracting lignin from lignocellulosic material using an aqueous organic solvent and an acid neutralizing agent patent, May 1988

Prehydrolysis of lignocellulose patent, April 1996

Simultaneous saccharification and fermentation of pretreated wheat straw to ethanol with selected yeast strains and β-glucosidase supplementation journal, January 1989

Method for the recovery of formic acid patent, June 2001

Evaluation of the cellobiose-fermenting yeastBrettanomyces custersii in the simultaneous saccharification and fermentation of cellulose
journal, May 1992

Fast and efficient alkaline peroxide treatment to enhance the enzymatic digestibility of steam-exploded softwood substrates
journal, January 2002

Investigation of the Cell-Wall Loosening Protein Expansin as a Possible Additive in the Enzymatic Saccharification of Lignocellulosic Biomass
journal, January 2000

The Reactions of Lignins with High Temperature Hydrogen Peroxide Part 2. The Oxidation of Kraft Lignin
journal, May 1999

Adsorption of high-purity endo-1,4-β-glucanases from Trichoderma reesei on components of lignocellulosic materials: Cellulose, lignin, and xylan
journal, August 1988

Process for making alcohol from cellulosic material using plural ferments
patent, February 1977

Continuous fermentation process for the production of metabolities using a moving filter
patent, November 1997

Factors affecting lignin degradation in lignocellulose by Phanerochaete chrysosporium
journal, February 1984

Two-stage dilute acid prehydrolysis of biomass
patent, June 1992

Fractionation of Lignocellulosics by Steam-Aqueous Pretreatments [and Discussion]
journal, April 1987

The simultaneous saccharification and fermentation of pretreated woody crops to ethanol
journal, March 1991

Delignification of lignocellulosic materials with peroxymonophosphoric acid
patent, June 1996

Cellulase Adsorption and an Evaluation of Enzyme Recycle During Hydrolysis of Steam-Exploded Softwood Residues
journal, January 2002

Mechanism of surfactant effect in enzymatic hydrolysis of lignocellulose
journal, August 2002

Method the hydrolytic splitting of acid treated comminuted crude cellulose with steam
patent, November 1987

High solids simultaneous saccharification and fermentation of pretreated wheat straw to ethanol
journal, May 1992

Simultaneous saccharification and fermentation (SSF) of lignocellulosics to ethanol under vacuum cycling and step feeding
journal, April 1984

Process for hydrolysis of biomass
patent, December 1985

Method of countercurrent acid hydrolysis of comminuted cellulosic fibrous material
patent, May 1987

Compositions and process for colored liquid food or drink
patent, September 1979

Increasing cellulose accessibility is more important than removing lignin: A comparison of cellulose solvent-based lignocellulose fractionation and soaking in aqueous ammonia
journal, September 2010

Dilute-acid pretreatment of two short-rotation herbaceous crops: Scientific Note
journal, March 1992

Continuous process for ethanol production from lignocellulosic materials without mechanical agitation
patent, November 1993

Evaluation of Candida acidothermophilum in ethanol production from lignocellulosic biomass
journal, December 1997

Hydrolysis and fractionation of lignocellulosic biomass
patent, February 2000

Method of treating biomass material
patent, July 1996

Bacteria for cellulose digestion
patent, August 1989

Thermotolerant yeast for simultaneous saccharification and fermentation of cellulose to ethanol
journal, April 1988

Process for pretreating cellulosic substrates and for producing sugar therefrom
patent, December 1980

Lignin Impact on Fiber Degradation. 3. Reversal of Inhibition of Enzymatic Hydrolysis by Chemical Modification of Lignin and by Additives
journal, May 1997

Biomass Ethanol: Technical Progress, Opportunities, and Commercial Challenges
journal, November 1999

Ethanol from lignocellulosic biomass: Technology, economics, and opportunities
journal, January 1994

Method of bioconversion of industrial or agricultural cellulose containing wastes
patent, December 2000

Simultaneous saccharification and fermentation of several lignocellulosic feedstocks to fuel ethanol
journal, January 1992

Optimization of Steam Explosion to Enhance Hemicellulose Recovery and Enzymatic Hydrolysis of Cellulose in Softwoods
journal, January 1999

Prehydrolysis of lignocellulose
patent, June 1995

Prehydrolysis of lignocellulose
patent, January 1998

Twenty Years of Trials, Tribulations, and Research Progress in Bioethanol Technology: Selected Key Events Along the Way
journal, January 2001

Continuous process for making ethanol
patent, November 1998

Weak Lignin-Binding Enzymes: A Novel Approach to Improve Activity of Cellulases for Hydrolysis of Lignocellulosics
journal, January 2005

Fuel Ethanol Production from Lignocellulose Using Genetically Engineered Bacteria
book, May 1997

Concentrated sulfuric acid hydrolysis of lignocellulosics
patent, May 2000

Evaluation of pretreated herbaceous crops for the simultaneous saccharification and fermentation process
journal, March 1990

Alkaline peroxide delignification of agricultural residues to enhance enzymatic saccharification
journal, January 1984

Process for extracting lignin from lignocellulosic material using an aqueous organic solvent and an acid neutralizing agent
patent, May 1988

Prehydrolysis of lignocellulose
patent, April 1996

Simultaneous saccharification and fermentation of pretreated wheat straw to ethanol with selected yeast strains and β-glucosidase supplementation
journal, January 1989

Method for the recovery of formic acid
patent, June 2001