Title: Nanoplasmonic molecular ruler for nuclease activity and DNA footprinting

Abstract

This invention provides a nanoplasmonic molecular ruler, which can perform label-free and real-time monitoring of nucleic acid (e.g., DNA) length changes and perform nucleic acid footprinting. In various embodiments the ruler comprises a nucleic acid attached to a nanoparticle, such that changes in the nucleic acid length are detectable using surface plasmon resonance. The nanoplasmonic ruler provides a fast and convenient platform for mapping nucleic acid-protein interactions, for nuclease activity monitoring, and for other footprinting related methods.

Inventors:

Chen, Fanqing Frank; Liu, Gang L; Lee, Luke P

Issue Date:

2013-10-29

Research Org.:

Lawrence Berkeley National Laboratory (LBNL), Berkeley, CA (United States)

Sponsoring Org.:

USDOE

OSTI Identifier:

1107635

Patent Number(s):

8569468

Application Number:

12/440,579

Assignee:

The Regents of the University of California (Oakland, CA)

Patent Classifications (CPCs):

C - CHEMISTRY C12 - BIOCHEMISTRY C12Q - MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS

G - PHYSICS G01 - MEASURING G01N - INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES

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C - CHEMISTRY C12 - BIOCHEMISTRY C12Q - MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS

C12Q1/6825 - Nucleic acid detection involving sensors
C12Q1/6827 - for detection of mutation or polymorphism
C12Q1/683 - involving restriction enzymes, e.g. restriction fragment length polymorphism [RFLP]
C12Q1/6834 - Enzymatic or biochemical coupling of nucleic acids to a solid phase
C12Q1/6876 - Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
C12Q2521/319 - Exonuclease
C12Q2563/155 - Particles of a defined size, e.g. nanoparticles
C12Q2565/628 - being a surface plasmon resonance spectrometer

G - PHYSICS G01 - MEASURING G01N - INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES

G01N21/554 - {detecting the surface plasmon resonance of nanostructured metals, e.g. localised surface plasmon resonance}

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DOE Contract Number:  

AC02-05CH11231

Resource Type:

Patent

Country of Publication:

United States

Language:

English

Subject:

59 BASIC BIOLOGICAL SCIENCES