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Title: Nanoplasmonic molecular ruler for nuclease activity and DNA footprinting

Abstract

This invention provides a nanoplasmonic molecular ruler, which can perform label-free and real-time monitoring of nucleic acid (e.g., DNA) length changes and perform nucleic acid footprinting. In various embodiments the ruler comprises a nucleic acid attached to a nanoparticle, such that changes in the nucleic acid length are detectable using surface plasmon resonance. The nanoplasmonic ruler provides a fast and convenient platform for mapping nucleic acid-protein interactions, for nuclease activity monitoring, and for other footprinting related methods.

Inventors:
; ;
Issue Date:
Research Org.:
Lawrence Berkeley National Laboratory (LBNL), Berkeley, CA (United States)
Sponsoring Org.:
USDOE
OSTI Identifier:
1107635
Patent Number(s):
8569468
Application Number:
12/440,579
Assignee:
The Regents of the University of California (Oakland, CA)
Patent Classifications (CPCs):
C - CHEMISTRY C12 - BIOCHEMISTRY C12Q - MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS
G - PHYSICS G01 - MEASURING G01N - INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
DOE Contract Number:  
AC02-05CH11231
Resource Type:
Patent
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES

Citation Formats

Chen, Fanqing Frank, Liu, Gang L, and Lee, Luke P. Nanoplasmonic molecular ruler for nuclease activity and DNA footprinting. United States: N. p., 2013. Web.
Chen, Fanqing Frank, Liu, Gang L, & Lee, Luke P. Nanoplasmonic molecular ruler for nuclease activity and DNA footprinting. United States.
Chen, Fanqing Frank, Liu, Gang L, and Lee, Luke P. Tue . "Nanoplasmonic molecular ruler for nuclease activity and DNA footprinting". United States. https://www.osti.gov/servlets/purl/1107635.
@article{osti_1107635,
title = {Nanoplasmonic molecular ruler for nuclease activity and DNA footprinting},
author = {Chen, Fanqing Frank and Liu, Gang L and Lee, Luke P},
abstractNote = {This invention provides a nanoplasmonic molecular ruler, which can perform label-free and real-time monitoring of nucleic acid (e.g., DNA) length changes and perform nucleic acid footprinting. In various embodiments the ruler comprises a nucleic acid attached to a nanoparticle, such that changes in the nucleic acid length are detectable using surface plasmon resonance. The nanoplasmonic ruler provides a fast and convenient platform for mapping nucleic acid-protein interactions, for nuclease activity monitoring, and for other footprinting related methods.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {2013},
month = {10}
}

Works referenced in this record:

Branched Coordination Multilayers on Gold
journal, November 2005


Magneitc-nanoparticle conjugates and methods of use
patent-application, May 2003


Conformation of Oligonucleotides Attached to Gold Nanocrystals Probed by Gel Electrophoresis
journal, January 2003


Enzyme-mediated individual nanoparticle release assay
journal, June 2006


Label-Free Detection of Peptide Nucleic Acid−DNA Hybridization Using Localized Surface Plasmon Resonance Based Optical Biosensor
journal, September 2005


One-Pot Colorimetric Differentiation of Polynucleotides with Single Base Imperfections Using Gold Nanoparticle Probes
journal, March 1998


A molecular ruler based on plasmon coupling of single gold and silver nanoparticles
journal, May 2005


Organization of 'nanocrystal molecules' using DNA
journal, August 1996


Sensitivity of Transmission Surface Plasmon Resonance (T-SPR) Spectroscopy: Self-Assembled Multilayers on Evaporated Gold Island Films
journal, September 2005


Nanoparticles having oligonucleotides attached thereto and uses therefor
patent, July 2007


Biosensing using surface plasmon resonance
patent, June 2003


Electrophoretic Isolation of Discrete Au Nanocrystal/DNA Conjugates
journal, January 2001


Comparison of DNA detection methods using nanoparticles and silver enhancement
journal, January 2005


Scanometric DNA Array Detection with Nanoparticle Probes
journal, September 2000