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Title: Method and apparatus for enhanced detection of toxic agents

Abstract

A biosensor based detection of toxins includes enhancing a fluorescence signal by concentrating a plurality of photosynthetic organisms in a fluid into a concentrated region using biased AC electro-osmosis. A measured photosynthetic activity of the photosynthetic organisms is obtained in the concentrated region, where chemical, biological or radiological agents reduce a nominal photosynthetic activity of the photosynthetic organisms. A presence of the chemical, biological and/or radiological agents or precursors thereof, is determined in the fluid based on the measured photosynthetic activity of the concentrated plurality of photosynthetic organisms. A lab-on-a-chip system is used for the concentrating step. The presence of agents is determined from feature vectors, obtained from processing a time dependent signal using amplitude statistics and/or time-frequency analysis, relative to a control signal. A linear discriminant method including support vector machine classification (SVM) is used to identify the agents.

Inventors:
; ; ;
Issue Date:
Research Org.:
Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States)
Sponsoring Org.:
USDOE
OSTI Identifier:
1107580
Patent Number(s):
8546073
Application Number:
13/474,183
Assignee:
UT-Battelle, LLC (Oak Ridge, TN)
Patent Classifications (CPCs):
C - CHEMISTRY C12 - BIOCHEMISTRY C12Q - MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS
DOE Contract Number:  
AC05-00OR22725
Resource Type:
Patent
Resource Relation:
Patent File Date: 2012 May 17
Country of Publication:
United States
Language:
English
Subject:
47 OTHER INSTRUMENTATION

Citation Formats

Greenbaum, Elias, Rodriguez, Jr., Miguel, Wu, Jie Jayne, and Qi, Hairong. Method and apparatus for enhanced detection of toxic agents. United States: N. p., 2013. Web.
Greenbaum, Elias, Rodriguez, Jr., Miguel, Wu, Jie Jayne, & Qi, Hairong. Method and apparatus for enhanced detection of toxic agents. United States.
Greenbaum, Elias, Rodriguez, Jr., Miguel, Wu, Jie Jayne, and Qi, Hairong. Tue . "Method and apparatus for enhanced detection of toxic agents". United States. https://www.osti.gov/servlets/purl/1107580.
@article{osti_1107580,
title = {Method and apparatus for enhanced detection of toxic agents},
author = {Greenbaum, Elias and Rodriguez, Jr., Miguel and Wu, Jie Jayne and Qi, Hairong},
abstractNote = {A biosensor based detection of toxins includes enhancing a fluorescence signal by concentrating a plurality of photosynthetic organisms in a fluid into a concentrated region using biased AC electro-osmosis. A measured photosynthetic activity of the photosynthetic organisms is obtained in the concentrated region, where chemical, biological or radiological agents reduce a nominal photosynthetic activity of the photosynthetic organisms. A presence of the chemical, biological and/or radiological agents or precursors thereof, is determined in the fluid based on the measured photosynthetic activity of the concentrated plurality of photosynthetic organisms. A lab-on-a-chip system is used for the concentrating step. The presence of agents is determined from feature vectors, obtained from processing a time dependent signal using amplitude statistics and/or time-frequency analysis, relative to a control signal. A linear discriminant method including support vector machine classification (SVM) is used to identify the agents.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {2013},
month = {10}
}

Works referenced in this record:

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Ultrasensitive magnetic biosensor for homogeneous immunoassay
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Long-Range AC Electroosmotic Trapping and Detection of Bioparticles
journal, April 2005


An AC Electrokinetic Technique for Collection and Concentration of Particles and Cells on Patterned Electrodes
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conference, January 2003


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Magnetic resonance as a technique to magnetic biosensors characterization in Neocapritermes opacus termites
journal, July 2005


Stand-off tissue-based biosensors for the detection of chemical warfare agents using photosynthetic fluorescence induction
journal, September 2001