DOE Patents title logo U.S. Department of Energy
Office of Scientific and Technical Information

Title: Transcription factor-based biosensor

Abstract

The present invention provides for a system comprising a BmoR transcription factor, a .sigma..sup.54-RNA polymerase, and a pBMO promoter operatively linked to a reporter gene, wherein the pBMO promoter is capable of expression of the reporter gene with an activated form of the BmoR and the .sigma..sup.54-RNA polymerase.

Inventors:
;
Issue Date:
Research Org.:
Lawrence Berkeley National Laboratory (LBNL), Berkeley, CA (United States)
Sponsoring Org.:
USDOE
OSTI Identifier:
1097260
Patent Number(s):
8552169
Application Number:
12/767,649
Assignee:
The Regents of the University of California (Oakland, CA)
Patent Classifications (CPCs):
C - CHEMISTRY C12 - BIOCHEMISTRY C12N - MICROORGANISMS OR ENZYMES
DOE Contract Number:  
AC02-05CH11231
Resource Type:
Patent
Country of Publication:
United States
Language:
English
Subject:
60 APPLIED LIFE SCIENCES

Citation Formats

Dietrich, Jeffrey A, and Keasling, Jay D. Transcription factor-based biosensor. United States: N. p., 2013. Web.
Dietrich, Jeffrey A, & Keasling, Jay D. Transcription factor-based biosensor. United States.
Dietrich, Jeffrey A, and Keasling, Jay D. Tue . "Transcription factor-based biosensor". United States. https://www.osti.gov/servlets/purl/1097260.
@article{osti_1097260,
title = {Transcription factor-based biosensor},
author = {Dietrich, Jeffrey A and Keasling, Jay D},
abstractNote = {The present invention provides for a system comprising a BmoR transcription factor, a .sigma..sup.54-RNA polymerase, and a pBMO promoter operatively linked to a reporter gene, wherein the pBMO promoter is capable of expression of the reporter gene with an activated form of the BmoR and the .sigma..sup.54-RNA polymerase.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {2013},
month = {10}
}

Works referenced in this record:

The Bacterial Enhancer-Dependent sigma 54 (sigma N) Transcription Factor
journal, August 2000


Product and product-independent induction of butane oxidation in Pseudomonas butanovora
journal, September 2005


In a class of its own ? the RNA polymerase sigma factor ?; 54 (? N )
journal, December 1993


Tight regulation, modulation, and high-level expression by vectors containing the arabinose PBAD promoter.
journal, July 1995


Microbial hybrid promoters
patent, November 1985


Involvement of BmoR and BmoG in n-alkane metabolism in ‘Pseudomonas butanovora’
journal, January 2008


A Propionate-Inducible Expression System for Enteric Bacteria
journal, November 2005