Methods of combined bioprocessing and related microorganisms, thermophilic and/or acidophilic enzymes, and nucleic acids encoding said enzymes

Thompson, David N; Apel, William A; Thompson, Vicki S; Ward, Thomas E

Title: Methods of combined bioprocessing and related microorganisms, thermophilic and/or acidophilic enzymes, and nucleic acids encoding said enzymes

Abstract

A genetically modified organism comprising: at least one nucleic acid sequence and/or at least one recombinant nucleic acid isolated from Alicyclobacillus acidocaldarius and encoding a polypeptide involved in at least partially degrading, cleaving, transporting, metabolizing, or removing polysaccharides, cellulose, lignocellulose, hemicellulose, lignin, starch, sugars, sugar oligomers, carbohydrates, complex carbohydrates, chitin, heteroxylans, glycosides, xylan-, glucan-, galactan-, or mannan-decorating groups; and at least one nucleic acid sequence and/or at least one recombinant nucleic acid encoding a polypeptide involved in fermenting sugar molecules to a product. Additionally, enzymatic and/or proteinaceous extracts may be isolated from one or more genetically modified organisms. The extracts are utilized to convert biomass into a product. Further provided are methods of converting biomass into products comprising: placing the genetically modified organism and/or enzymatic extracts thereof in fluid contact with polysaccharides, cellulose, lignocellulose, hemicellulose, lignin, starch, sugars, sugar oligomers, carbohydrates, complex carbohydrates, chitin, heteroxylans, glycosides, and/or xylan-, glucan-, galactan-, or mannan-decorating groups.

Inventors:: Thompson, David N; Apel, William A; Thompson, Vicki S; Ward, Thomas E

Issue Date:: 2013-07-23

Research Org.:: Idaho National Laboratory (INL), Idaho Falls, ID (United States)

Sponsoring Org.:: USDOE

OSTI Identifier:: 1088690

Patent Number(s):: 8492114

Application Number:: 12/587,229

Assignee:: Battelle Energy Alliance, LLC (Idaho Falls, ID)

Patent Classifications (CPCs):: C - CHEMISTRY C12 - BIOCHEMISTRY C12N - MICROORGANISMS OR ENZYMES

C - CHEMISTRY C12 - BIOCHEMISTRY C12P - FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE {

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C - CHEMISTRY C12 - BIOCHEMISTRY C12N - MICROORGANISMS OR ENZYMES
C12N15/52 - Genes encoding for enzymes or proenzymes

C - CHEMISTRY C12 - BIOCHEMISTRY C12P - FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE {
C12P19/02 - Monosaccharides
C12P19/14 - produced by the action of a carbohydrase {
C12P7/065 - {with microorganisms other than yeasts}
C12P7/10 - substrate containing cellulosic material
C12P7/14 - Multiple stages of fermentation

Y - NEW / CROSS SECTIONAL TECHNOLOGIES Y02 - TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE Y02E - REDUCTION OF GREENHOUSE GAS [GHG] EMISSIONS, RELATED TO ENERGY GENERATION, TRANSMISSION OR DISTRIBUTION
Y02E50/10 - Biofuels

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DOE Contract Number:: AC07-99ID13727; AC07-05ID14517

Resource Type:: Patent

Country of Publication:: United States

Language:: English

Subject:: 59 BASIC BIOLOGICAL SCIENCES

Citation Formats


                    Thompson, David N, Apel, William A, Thompson, Vicki S, and Ward, Thomas E. Methods of combined bioprocessing and related microorganisms, thermophilic and/or acidophilic enzymes, and nucleic acids encoding said enzymes.  United States: N. p., 2013. 
        Web.

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                    Thompson, David N, Apel, William A, Thompson, Vicki S, & Ward, Thomas E. Methods of combined bioprocessing and related microorganisms, thermophilic and/or acidophilic enzymes, and nucleic acids encoding said enzymes.  United States.

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                    Thompson, David N, Apel, William A, Thompson, Vicki S, and Ward, Thomas E. Tue .  
        "Methods of combined bioprocessing and related microorganisms, thermophilic and/or acidophilic enzymes, and nucleic acids encoding said enzymes".  United States.  https://www.osti.gov/servlets/purl/1088690.

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@article{osti_1088690,

  title        = {Methods of combined bioprocessing and related microorganisms, thermophilic and/or acidophilic enzymes, and nucleic acids encoding said enzymes},

  author       = {Thompson, David N and Apel, William A and Thompson, Vicki S and Ward, Thomas E},

  abstractNote = {A genetically modified organism comprising: at least one nucleic acid sequence and/or at least one recombinant nucleic acid isolated from Alicyclobacillus acidocaldarius and encoding a polypeptide involved in at least partially degrading, cleaving, transporting, metabolizing, or removing polysaccharides, cellulose, lignocellulose, hemicellulose, lignin, starch, sugars, sugar oligomers, carbohydrates, complex carbohydrates, chitin, heteroxylans, glycosides, xylan-, glucan-, galactan-, or mannan-decorating groups; and at least one nucleic acid sequence and/or at least one recombinant nucleic acid encoding a polypeptide involved in fermenting sugar molecules to a product. Additionally, enzymatic and/or proteinaceous extracts may be isolated from one or more genetically modified organisms. The extracts are utilized to convert biomass into a product. Further provided are methods of converting biomass into products comprising: placing the genetically modified organism and/or enzymatic extracts thereof in fluid contact with polysaccharides, cellulose, lignocellulose, hemicellulose, lignin, starch, sugars, sugar oligomers, carbohydrates, complex carbohydrates, chitin, heteroxylans, glycosides, and/or xylan-, glucan-, galactan-, or mannan-decorating groups.},

  doi          = {},

  journal      = {},
number       = ,

  volume       = ,

  place        = {United States},

  year         = {2013},

  month        = {7}

}

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Schäfer, Karsten; Magnusson, Ulrika; Scheffel, Frank
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Measurement of fumonisins in corn with a fiber optic fluoroimmunosensor
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Thompson, Vicki S.; Maragos, Chris M.; Thompson, Richard B.
Advances in Fluorescence Sensing Technology III, Vol. 2980
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Purification and Characterization of a Novel Thermo-Alkali-Stable Catalase from Thermus brockianus
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Thompson, Vicki S.; Schaller, Kastli D.; Apel, William A.
Biotechnology Progress, Vol. 19, Issue 4, p. 1292-1299
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Genes encoding two different beta-glucosidases of Thermoanaerobacter brockii are clustered in a common operon.
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Breves, R.; Bronnenmeier, K.; Wild, N.
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Saha, Badal C.; Iten, Loren B.; Cotta, Michael A.
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The Path Forward for Biofuels and Biomaterials
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Ragauskas, Arthur J.; Williams, Charlotte K.; Davison, Brian H.
Science, Vol. 311, Issue 5760, p. 484-489
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Alteration and modulation of protein activity by varying post-translational modification
patent-application, August 2010

Thompson, David N.; Reed, David W.; Thompson, Vicki S.
US Patent Application 12/655993; 20100203583
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Xylanase derived from a Bacillus species, expression vectors for such xylanase and other proteins, host organisms therefor and use thereof
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De Buyl, Eric; Lahaye, Andrée; Ledoux, Pierre
US Patent Document 6,426,211
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Knappert, Diane; Grethlein, Hans; Converse, Alvin
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Hamelinck, Carlo N; Hooijdonk, Geertje van; Faaij, André PC
Biomass and Bioenergy, Vol. 28, Issue 4, p. 384-410
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Thermostable xylanases
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Gronberg, Vidar; Forster, Simon; Moody, Dean
US Patent Document 6,083,733
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Melamine Deaminase and Atrazine Chlorohydrolase: 98 Percent Identical but Functionally Different
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Seffernick, J. L.; de Souza, M. L.; Sadowsky, M. J.
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Broun, Pierre; Shanklin, John; Whittle, Ed
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Liu, Chaogang; Wyman, Charles E.
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Ward, Thomas E.; Eynard, Natalie; Teissié, Justin
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Buckholz, Richard G.
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Pajunen, M. I.
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Ooshima, Hiroshi; Ishitani, Yohsuke; Harano, Yoshio
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The acid hydrolysis of sugarcane bagasse hemicellulose to produce xylose, arabinose, glucose and other products
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Lavarack, B. P.; Griffin, G. J.; Rodman, D.
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Schulein, Martin; Outtrup, Helle; Jorgensen, Per Lina
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Ramos, Juan; González, Mercedes; Ramírez, Felipe
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Bergquist, Peter L.; Gibbs, Moreland D.; Morris, Daniel D.
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Turner, Pernilla; Mamo, Gashaw; Karlsson, Eva
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Fungal Upgrading of Wheat Straw for Straw-Thermoplastics Production
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Houghton, Tracy P.; Thompson, David N.; Hess, J. Richard
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Bailey, Michael J.; Biely, Peter; Poutanen, Kaisa
Journal of Biotechnology, Vol. 23, Issue 3, p. 257-270
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Crout, David H. G.; Vic, Gabin
Current Opinion in Chemical Biology, Vol. 2, Issue 1, p. 98-111
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Enzyme and methodology for the treatment of a biomass
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Thompson, Vicki S.; Thompson, David N.; Schaller, Kastli D.
US Patent Document 7,727,755
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Purification, Properties and Structural Aspects of a Thermoacidophilic alpha-Amylase from Alicyclobacillus Acidocaldarius Atcc 27009
journal, December 1994

Schwermann, Birgit; Pfau, Karsten; Liliensiek, Birgit
European Journal of Biochemistry, Vol. 226, Issue 3, p. 981-991
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Gene cloning, sequencing, and characterization of a family 9 endoglucanase (CelA) with an unusual pattern of activity from the thermoacidophile Alicyclobacillus acidocaldarius ATCC27009
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K., Eckert; F., Zielinski; Leggio, L. Lo
Applied Microbiology and Biotechnology, Vol. 60, Issue 4, p. 428-436
https://doi.org/10.1007/s00253-002-1131-4

Industrial Scale-Up of pH-Controlled Liquid Hot Water Pretreatment of Corn Fiber for Fuel Ethanol Production
journal, January 2005

Mosier, Nathan S.; Hendrickson, Richard; Brewer, Mark
Applied Biochemistry and Biotechnology, Vol. 125, Issue 2, p. 77-98
https://doi.org/10.1385/ABAB:125:2:077

Purification and characterization of an acidophilic xylanase from Aureobasidium pullulans var. melanigenum and sequence analysis of the encoding gene
journal, January 2001

Ohta, Kazuyoshi; Moriyama, Satoshi; Tanaka, Hidenori
Journal of Bioscience and Bioengineering, Vol. 92, Issue 3, p. 262-270
https://doi.org/10.1016/S1389-1723(01)80260-7

Purification and Properties of Two Thermostable Alkaline Xylanases from an Alkaliphilic Bacillussp
journal, September 1998

Gessesse, Amare
Applied and Environmental Microbiology, Vol. 64, Issue 9
https://doi.org/10.1128/AEM.64.9.3533-3535.1998

Deciphering the message in protein sequences: tolerance to amino acid substitutions
journal, March 1990

Bowie, J.; Reidhaar-Olson, J.; Lim, W.
Science, Vol. 247, Issue 4948, p. 1306-1310
https://doi.org/10.1126/science.2315699

Cloning and characterization of two glycosidases from the acidothermophile Alicyclobacillus acidocaldarius ATCC27009
text, January 2004

Eckert, Kelvin
Humboldt-Universität zu Berlin, Mathematisch-Naturwissenschaftliche Fakultät I
https://doi.org/10.18452/15013

Thermophilic and thermoacidophilic biopolymer-degrading genes and enzymes from alicyclobacillus acidocaldarius and related organisms, methods
patent-application, August 2009

Thompson, David N.; Apel, William A.; Thompson, Vicki S.
US Patent Apllication 12/322359; 20090203107
URL: https://image-ppubs.uspto.gov/dirsearch-public/print/downloadPdf/20090203107

Thermostable xylanase
patent, June 1999

Fukunaga, Nobuyuki; Iwasaki, Yuji; Kono, Satoko
US Patent Document 5,916,795
URL: https://image-ppubs.uspto.gov/dirsearch-public/print/downloadPdf/5916795

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Title: Methods of combined bioprocessing and related microorganisms, thermophilic and/or acidophilic enzymes, and nucleic acids encoding said enzymes

Abstract

Citation Formats

The first description of an archaeal hemicellulase: the xylanase from Thermococcus zilligii strain AN1 journal, November 1999

Expression of microbial proteins in plants for production of plants with improved properties patent-application, December 2003

Process for hydrolyzing starch without pH adjustment patent-application, July 2003

The nature of lignocellulosics and their pretreatments for enzymatic hydrolysis book, January 1982

PCR ligation mutagenesis in transformable streptococci: application and efficiency journal, April 2002

Conversion of a β-Ketoacyl Synthase to a Malonyl Decarboxylase by Replacement of the Active-Site Cysteine with Glutamine journal, September 1999

Production of a cellulase-free xylanase from agricultural waste materials by a thermotolerant Streptomyces sp. journal, October 2001

Glycosylation Engineering: Controlling personalities tame wild sugars on proteins and natural products journal, September 2006

Lignochemicals book, January 1982

Probe containing a modified nucleic acid recognizable by specific antibodies and use of this probe to detect and characterize a homologous dna sequence patent, March 1992

Thermal and acid tolerant beta-xylosidases, genes encoding, related organisms, and methods patent-application, September 2009

Thermophilic Organisms For Conversion Of Lignocellulosic Biomass To Ethanol patent-application, September 2009

Purification and Properties of Acid Stable Xylanases from Aspergillus kawachii journal, January 1992

Xylanases in bleaching: From an idea to the industry journal, March 1994

Synthesis of oligosaccharides, glycolipds, and glycoproteins using bacterial glycosyltransferases patent-application, June 2007

Isolation and characterization of a new family 42 β-galactosidase from the thermoacidophilic bacterium Alicyclobacillus acidocaldarius: Identification of the active site residues journal, February 2008

Maltose and Maltodextrin Transport in the Thermoacidophilic Gram-Positive Bacterium Alicyclobacillus acidocaldarius Is Mediated by a High-Affinity Transport System That Includes a Maltose Binding Protein Tolerant to Low pH journal, November 2000

journal, October 2003

Xylanase obtained from an anaerobic fungus patent, September 1999

Strand displacement amplification—an isothermal, in vitro DNA amplification technique journal, January 1992

Molecular and biotechnological aspects of xylanases journal, July 1999

Comparison of the economics of acid and enzymatic hydrolysis of newsprint journal, April 1978

Baculovirus systems for the expression of human gene products journal, October 1993

Multiple Chromosomes in Bacteria: The Yin and Yang of trp Gene Localization in Rhodobacter sphaeroides 2.4.1 journal, October 1999

Continuous cultures of fused cells secreting antibody of predefined specificity journal, August 1975

Genetic disease detection and DNA amplification using cloned thermostable ligase. journal, January 1991

Tomato plants that exhibit resistance to botrytis cinerea patent-application, December 2003

Thermophilic and thermoacidophilic sugar transporter genes and enzymes from alicyclobacillus acidocaldarius and related organisms, methods patent-application, October 2009

Manufacture of Xylose-Based Fermentation Media from Corncobs by Posthydrolysis of Autohydrolysis Liquors journal, January 2001

Microbial process for producing L-ascorbic acid, D-erythorbic acid, and salts thereof patent, August 2004

A thermoacidophilic endoglucanase (CelB) from Alicyclobacillus acidocaldarius displays high sequence similarity to arabinofuranosidases belonging to family 51 of glycoside hydrolases journal, August 2003

Biochemistry and genetics of microbial xylanases journal, June 1996

Analytical strategies for the use of DNA probes journal, February 1988

Plasmid, method for construction of the same, microorganism carrying the plasmid and method for cultivation of the microorganism patent, November 1986

Process for pretreating cellulosic substrates and for producing sugar therefrom patent, December 1980

High temperature and alkaline stable catalase patent-application, May 2005

Thermophilic and thermoacidophilic glycosylation genes and enzymes from Alicyclobacillus acidocaldarius and related organisms, methods patent-application, October 2009

Identification of essential genes in microorganisms patent-application, February 2004

Preliminary Investigation of Fungal Bioprocessing of Wheat Straw for Production of Straw-Thermoplastic Composites book, January 2003

Cellulase-free xylanases from Bacillus and other microorganisms journal, February 2000

Single molecule detection of RNA reporter probes by amplification with Qβ replicase journal, August 1996

Functional reconstitution of a maltose ATP-binding cassette transporter from the thermoacidophilic gram-positive bacterium Alicyclobacillus acidocaldarius journal, May 2004

Bacterial glycoproteins: Functions, biosynthesis and applications journal, April 2003

Overview and Evaluation of Fuel Ethanol from Cellulosic Biomass: Technology, Economics, the Environment, and Policy journal, November 1996

Rapid production of thermostable cellulase-free xylanase by a strain of Bacillus subtilis and its properties journal, June 2002

Microbial β-Glucosidases: Cloning, Properties, and Applications journal, January 2002

Crystallographic and mutational analyses of an extremely acidophilic and acid-stable xylanase: biased distribution of acidic residues and importance of Asp37 for catalysis at low pH journal, December 1998

Synthesis of an amplifiable reporter RNA for bioassays journal, January 1986

Characterization of a new bacteriophage which infects bacteria of the genus Acidiphilium journal, November 1993

Genetic elements, proteins, and associated methods including application of additional genetic information to gram (+) thermoacidophiles patent-application, November 2011

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Isothermal in vitro amplification of DNA by a restriction enzyme/DNA polymerase system. journal, January 1992

Autocatalytic replication of a recombinant RNA journal, December 1983

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Polymerase chain reaction journal, November 1989

Transformation of Acidiphilium by electroporation and conjugation journal, May 1992

Thermal and acid tolerant beta xylosidases, arabinofuranosidases, genes encoding, related organisms, and methods patent-application, December 2010

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Thermoacidophilic Microorganisms and their Novel Biocatalysts journal, December 2004

Method of detecting and characterizing a nucleic acid or reactant for the application of this method patent, April 1986

Products and processes for in vitro synthesis of biomolecules patent-application, September 2006

Powers and Pitfalls in Sequence Analysis: The 70% Hurdle journal, April 2000

Dilute-Sulfuric Acid Pretreatment of Corn Stover in Pilot-Scale Reactor: Investigation of Yields, Kinetics, and Enzymatic Digestibilities of Solids journal, January 2003

Purification, characterization, and gene cloning of thermopsin, a thermostable acid protease from Sulfolobus acidocaldarius. journal, January 1990

Polypeptides having haloperoxidase activity patent, January 2003

Recent advances in heterologous gene expression in Escherichia coli journal, October 1993

Compositions and methods for the synthesis and subsequent modification of uridine-5'-diphosphosulfoquinovose (UDP-SQ) patent-application, June 2007

Promoters and proteins from Clostridium thermocellum and uses thereof patent-application, May 2006

Thermophilic and thermoacidophilic metabolism genes and enzymes from alicyclobacillus acidocaldadarius and related organisms, methods patent-application, October 2009

Xylanases, xylanase families and extremophilic xylanases journal, January 2005

X-ray Structures of the Maltose–Maltodextrin-binding Protein of the Thermoacidophilic Bacterium Alicyclobacillus acidocaldarius Provide Insight into Acid Stability of Proteins journal, January 2004

Measurement of fumonisins in corn with a fiber optic fluoroimmunosensor conference, May 1997

Purification and Characterization of a Novel Thermo-Alkali-Stable Catalase from Thermus brockianus journal, January 2003

The first description of an archaeal hemicellulase: the xylanase from Thermococcus zilligii strain AN1
journal, November 1999

Expression of microbial proteins in plants for production of plants with improved properties
patent-application, December 2003

Process for hydrolyzing starch without pH adjustment
patent-application, July 2003

The nature of lignocellulosics and their pretreatments for enzymatic hydrolysis
book, January 1982

PCR ligation mutagenesis in transformable streptococci: application and efficiency
journal, April 2002

Conversion of a β-Ketoacyl Synthase to a Malonyl Decarboxylase by Replacement of the Active-Site Cysteine with Glutamine
journal, September 1999

Production of a cellulase-free xylanase from agricultural waste materials by a thermotolerant Streptomyces sp.
journal, October 2001

Glycosylation Engineering: Controlling personalities tame wild sugars on proteins and natural products
journal, September 2006

Lignochemicals
book, January 1982

Probe containing a modified nucleic acid recognizable by specific antibodies and use of this probe to detect and characterize a homologous dna sequence
patent, March 1992

Thermal and acid tolerant beta-xylosidases, genes encoding, related organisms, and methods
patent-application, September 2009

Thermophilic Organisms For Conversion Of Lignocellulosic Biomass To Ethanol
patent-application, September 2009

Purification and Properties of Acid Stable Xylanases from Aspergillus kawachii
journal, January 1992

Xylanases in bleaching: From an idea to the industry
journal, March 1994

Synthesis of oligosaccharides, glycolipds, and glycoproteins using bacterial glycosyltransferases
patent-application, June 2007

Isolation and characterization of a new family 42 β-galactosidase from the thermoacidophilic bacterium Alicyclobacillus acidocaldarius: Identification of the active site residues
journal, February 2008

Maltose and Maltodextrin Transport in the Thermoacidophilic Gram-Positive Bacterium Alicyclobacillus acidocaldarius Is Mediated by a High-Affinity Transport System That Includes a Maltose Binding Protein Tolerant to Low pH
journal, November 2000

Xylanase obtained from an anaerobic fungus
patent, September 1999

Strand displacement amplification—an isothermal, in vitro DNA amplification technique
journal, January 1992

Molecular and biotechnological aspects of xylanases
journal, July 1999

Comparison of the economics of acid and enzymatic hydrolysis of newsprint
journal, April 1978

Baculovirus systems for the expression of human gene products
journal, October 1993

Multiple Chromosomes in Bacteria: The Yin and Yang of trp Gene Localization in Rhodobacter sphaeroides 2.4.1
journal, October 1999

Continuous cultures of fused cells secreting antibody of predefined specificity
journal, August 1975

Genetic disease detection and DNA amplification using cloned thermostable ligase.
journal, January 1991

Tomato plants that exhibit resistance to botrytis cinerea
patent-application, December 2003

Thermophilic and thermoacidophilic sugar transporter genes and enzymes from alicyclobacillus acidocaldarius and related organisms, methods
patent-application, October 2009

Manufacture of Xylose-Based Fermentation Media from Corncobs by Posthydrolysis of Autohydrolysis Liquors
journal, January 2001

Microbial process for producing L-ascorbic acid, D-erythorbic acid, and salts thereof
patent, August 2004

A thermoacidophilic endoglucanase (CelB) from Alicyclobacillus acidocaldarius displays high sequence similarity to arabinofuranosidases belonging to family 51 of glycoside hydrolases
journal, August 2003

Biochemistry and genetics of microbial xylanases
journal, June 1996

Analytical strategies for the use of DNA probes
journal, February 1988

Plasmid, method for construction of the same, microorganism carrying the plasmid and method for cultivation of the microorganism
patent, November 1986

Process for pretreating cellulosic substrates and for producing sugar therefrom
patent, December 1980

High temperature and alkaline stable catalase
patent-application, May 2005

Thermophilic and thermoacidophilic glycosylation genes and enzymes from Alicyclobacillus acidocaldarius and related organisms, methods
patent-application, October 2009

Identification of essential genes in microorganisms
patent-application, February 2004

Preliminary Investigation of Fungal Bioprocessing of Wheat Straw for Production of Straw-Thermoplastic Composites
book, January 2003

Cellulase-free xylanases from Bacillus and other microorganisms
journal, February 2000

Single molecule detection of RNA reporter probes by amplification with Qβ replicase
journal, August 1996

Functional reconstitution of a maltose ATP-binding cassette transporter from the thermoacidophilic gram-positive bacterium Alicyclobacillus acidocaldarius
journal, May 2004

Bacterial glycoproteins: Functions, biosynthesis and applications
journal, April 2003

Overview and Evaluation of Fuel Ethanol from Cellulosic Biomass: Technology, Economics, the Environment, and Policy
journal, November 1996

Rapid production of thermostable cellulase-free xylanase by a strain of Bacillus subtilis and its properties
journal, June 2002

Microbial β-Glucosidases: Cloning, Properties, and Applications
journal, January 2002

Crystallographic and mutational analyses of an extremely acidophilic and acid-stable xylanase: biased distribution of acidic residues and importance of Asp37 for catalysis at low pH
journal, December 1998

Synthesis of an amplifiable reporter RNA for bioassays
journal, January 1986

Characterization of a new bacteriophage which infects bacteria of the genus Acidiphilium
journal, November 1993

Genetic elements, proteins, and associated methods including application of additional genetic information to gram (+) thermoacidophiles
patent-application, November 2011

Oxygen effects on thermophilic microbial populations in biofilters treating nitric oxide containing off-gas streams
journal, October 2001

‘Pseudomonas stutzeri’ strain and process for preparation of xylanase
patent, December 2004

Transcriptional control in alicyclobacillus acidocaldarius and associated genes, proteins, and methods
patent-application, August 2009

Thermostable .alpha.-L-arabinofuranosidase from Aureobasidium pullulans
patent, March 1999

Current applications of COS cell based transient expression systems
journal, October 1993

Isothermal in vitro amplification of DNA by a restriction enzyme/DNA polymerase system.
journal, January 1992

Autocatalytic replication of a recombinant RNA
journal, December 1983

Polypeptides of Alicyclobacillus sp.
patent-application, April 2007

Polymerase chain reaction
journal, November 1989

Transformation of Acidiphilium by electroporation and conjugation
journal, May 1992

Thermal and acid tolerant beta xylosidases, arabinofuranosidases, genes encoding, related organisms, and methods
patent-application, December 2010

An extremely thermostable xylanase from the thermophilic eubacterium Thermotoga
journal, July 1991

Thermoacidophilic Microorganisms and their Novel Biocatalysts
journal, December 2004

Method of detecting and characterizing a nucleic acid or reactant for the application of this method
patent, April 1986

Products and processes for in vitro synthesis of biomolecules
patent-application, September 2006

Powers and Pitfalls in Sequence Analysis: The 70% Hurdle
journal, April 2000

Dilute-Sulfuric Acid Pretreatment of Corn Stover in Pilot-Scale Reactor: Investigation of Yields, Kinetics, and Enzymatic Digestibilities of Solids
journal, January 2003

Purification, characterization, and gene cloning of thermopsin, a thermostable acid protease from Sulfolobus acidocaldarius.
journal, January 1990

Polypeptides having haloperoxidase activity
patent, January 2003

Recent advances in heterologous gene expression in Escherichia coli
journal, October 1993

Compositions and methods for the synthesis and subsequent modification of uridine-5'-diphosphosulfoquinovose (UDP-SQ)
patent-application, June 2007

Promoters and proteins from Clostridium thermocellum and uses thereof
patent-application, May 2006

Thermophilic and thermoacidophilic metabolism genes and enzymes from alicyclobacillus acidocaldadarius and related organisms, methods
patent-application, October 2009

Xylanases, xylanase families and extremophilic xylanases
journal, January 2005

X-ray Structures of the Maltose–Maltodextrin-binding Protein of the Thermoacidophilic Bacterium Alicyclobacillus acidocaldarius Provide Insight into Acid Stability of Proteins
journal, January 2004

Measurement of fumonisins in corn with a fiber optic fluoroimmunosensor
conference, May 1997

Purification and Characterization of a Novel Thermo-Alkali-Stable Catalase from Thermus brockianus
journal, January 2003

Genes encoding two different beta-glucosidases of Thermoanaerobacter brockii are clustered in a common operon.
journal, January 1997

Specificity of the protein secretory apparatus: secretion of the heat-labile enterotoxin B subunit pentamers by different species of Gram- bacteria
journal, January 1995

Dilute Acid Pretreatment, Enzymatic Saccharification, and Fermentation of Rice Hulls to Ethanol
journal, January 2005

Development of gas phase bioreactors for the removal of nitrogen oxides from synthetic flue gas streams
journal, October 2002