Genetically modified yeast species, and fermentation processes using genetically modified yeast
Abstract
Yeast cells are transformed with an exogenous xylose isomerase gene. Additional genetic modifications enhance the ability of the transformed cells to ferment xylose to ethanol or other desired fermentation products. Those modifications include deletion of non-specific or specific aldose reductase gene(s), deletion of xylitol dehydrogenase gene(s) and/or overexpression of xylulokinase.
- Inventors:
- Issue Date:
- Research Org.:
- Cargill, Incorporated (Wayzata, MN)
- Sponsoring Org.:
- USDOE
- OSTI Identifier:
- 1084065
- Patent Number(s):
- 8440451
- Application Number:
- 13/107,881
- Assignee:
- Cargill, Incorporated (Wayzata, MN)
- Patent Classifications (CPCs):
-
C - CHEMISTRY C12 - BIOCHEMISTRY C12N - MICROORGANISMS OR ENZYMES
C - CHEMISTRY C12 - BIOCHEMISTRY C12P - FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE {
- DOE Contract Number:
- FC07-02ID14349
- Resource Type:
- Patent
- Country of Publication:
- United States
- Language:
- English
- Subject:
- 59 BASIC BIOLOGICAL SCIENCES
Citation Formats
Rajgarhia, Vineet, Koivuranta, Kari, Penttila, Merja, Ilmen, Marja, Suominen, Pirkko, Aristidou, Aristos, Miller, Christopher Kenneth, Olson, Stacey, and Ruohonen, Laura. Genetically modified yeast species, and fermentation processes using genetically modified yeast. United States: N. p., 2013.
Web.
Rajgarhia, Vineet, Koivuranta, Kari, Penttila, Merja, Ilmen, Marja, Suominen, Pirkko, Aristidou, Aristos, Miller, Christopher Kenneth, Olson, Stacey, & Ruohonen, Laura. Genetically modified yeast species, and fermentation processes using genetically modified yeast. United States.
Rajgarhia, Vineet, Koivuranta, Kari, Penttila, Merja, Ilmen, Marja, Suominen, Pirkko, Aristidou, Aristos, Miller, Christopher Kenneth, Olson, Stacey, and Ruohonen, Laura. Tue .
"Genetically modified yeast species, and fermentation processes using genetically modified yeast". United States. https://www.osti.gov/servlets/purl/1084065.
@article{osti_1084065,
title = {Genetically modified yeast species, and fermentation processes using genetically modified yeast},
author = {Rajgarhia, Vineet and Koivuranta, Kari and Penttila, Merja and Ilmen, Marja and Suominen, Pirkko and Aristidou, Aristos and Miller, Christopher Kenneth and Olson, Stacey and Ruohonen, Laura},
abstractNote = {Yeast cells are transformed with an exogenous xylose isomerase gene. Additional genetic modifications enhance the ability of the transformed cells to ferment xylose to ethanol or other desired fermentation products. Those modifications include deletion of non-specific or specific aldose reductase gene(s), deletion of xylitol dehydrogenase gene(s) and/or overexpression of xylulokinase.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {Tue May 14 00:00:00 EDT 2013},
month = {Tue May 14 00:00:00 EDT 2013}
}
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Works referenced in this record:
Transformation in fungi.
journal, January 1989
- Fincham, J. R.
- Microbiological Reviews, Vol. 53, Issue 1
Processes for preparing tertiary aminoalkylorganometallic compounds
patent, November 2002
- Schwindeman, James A.; Hall, Randy W.; Stryker, Sonia S.
- US Patent Document 6,482,944
Molecular and industrial aspects of glucose isomerase.
journal, January 1996
- Bhosale, S. H.; Rao, M. B.; Deshpande, V. V.
- Microbiological reviews, Vol. 60, Issue 2
Xylulokinase Overexpression in Two Strains of Saccharomyces cerevisiae Also Expressing Xylose Reductase and Xylitol Dehydrogenase and Its Effect on Fermentation of Xylose and Lignocellulosic Hydrolysate
journal, September 2001
- Johansson, B.; Christensson, C.; Hobley, T.
- Applied and Environmental Microbiology, Vol. 67, Issue 9
Genetically engineered yeast and mutants thereof for the efficient fermentation of lignocellulose hydrolysates
patent, June 2002
- Träff, K. L.; Cordero Otero, R. R.; Van Zyl, W. H.
- US Patent Document 6,410,302
Xylose isomerase activity influences xylose fermentation with recombinant Saccharomyces cerevisiae strains expressing mutated xylA from Thermus thermophilus
journal, April 2003
- Lönn, A.; Träff-Bjerre, K. L.; Cordero Otero, R. R.
- Enzyme and Microbial Technology, Vol. 32, Issue 5
Minimal metabolic engineering of for efficient anaerobic xylose fermentation a proof of principle
journal, March 2004
- Kuyper, Marco; Winkler, Aaron; van Dijken, Johannes
- FEMS Yeast Research, Vol. 4, Issue 6, p. 655-664
Xylose utilization by recombinant yeasts
patent, February 1999
- Hallborn, Johan; Penttila, Merja; Ojamo, Heikki
- US Patent Document 5,866,382
Xylose metabolism in the anaerobic fungus Piromyces sp. strain E2 follows the bacterial pathway
journal, June 2003
- Harhangi, Harry R.; Akhmanova, Anna S.; Emmens, Roul
- Archives of Microbiology, Vol. 180, Issue 2
Yeast vectors for integration at the HO locus
journal, June 2001
- Voth, W. P.
- Nucleic Acids Research, Vol. 29, Issue 12
Xylose isomerase with improved properties
patent, November 2002
- Otero, Ricardo Román Cordero; Gárdonyi, Márk; Hahn-Hägerdal, Bärbel
- US Patent Document 6,475,768
Role of D-ribose as a cometabolite in D-xylose metabolism by Saccharomyces cerevisiae
journal, May 1993
- van Zyl, C.; Prior, B. A.; Kilian, S. G.
- Applied and Environmental Microbiology, Vol. 59, Issue 5
Isolation and characterization of acetic acid-tolerant galactose-fermenting strains of Saccharomyces cerevisiae from a spent sulfite liquor fermentation plant.
journal, January 1992
- Lindén, T.; Peetre, J.; Hahn-Hägerdal, B.
- Applied and Environmental Microbiology, Vol. 58, Issue 5
Amino Acid Substitutions in the Yeast Pichia Stipitis Xylitol Dehydrogenase Coenzyme-Binding Domain Affect the Coenzyme Specificity
journal, February 1995
- Metzger, Markus H.; Hollenberg, Cornells P.
- European Journal of Biochemistry, Vol. 228, Issue 1
High-level functional expression of a fungal xylose isomerase: the key to efficient ethanolic fermentation of xylose by ?
journal, October 2003
- Kuyper, M.; Harhangi, H.; Stave, A.
- FEMS Yeast Research, Vol. 4, Issue 1
Deletion of the GRE3 Aldose Reductase Gene and Its Influence on Xylose Metabolism in Recombinant Strains of Saccharomyces cerevisiae Expressing the xylA and XKS1 Genes
journal, December 2001
- Traff, K. L.; Otero Cordero, R. R.; van Zyl, W. H.
- Applied and Environmental Microbiology, Vol. 67, Issue 12