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Title: Selective posttranslational modification of phage-displayed polypeptides

The invention relates to posttranslational modification of phage-displayed polypeptides. These displayed polypeptides comprise at least one unnatural amino acid, e.g., an aryl-azide amino acid such as p-azido-L-phenylalanine, or an alkynyl-amino acid such as para-propargyloxyphenylalanine, which are incorporated into the phage-displayed fusion polypeptide at a selected position by using an in vivo orthogonal translation system comprising a suitable orthogonal aminoacyl-tRNA synthetase and a suitable orthogonal tRNA species. These unnatural amino acids advantageously provide targets for posttranslational modifications such as azide-alkyne [3+2]cycloaddition reactions and Staudinger modifications.
Inventors:
; ;
Issue Date:
OSTI Identifier:
1083220
Assignee:
The Scripps Research Institute (La Jolla, CA) DOEEE
Patent Number(s):
8,367,588
Application Number:
11/992,341
Research Org:
The Scripps Research Institute (La Jolla, CA)
Sponsoring Org:
USDOE
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES

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