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Title: Selective posttranslational modification of phage-displayed polypeptides

Abstract

The invention relates to posttranslational modification of phage-displayed polypeptides. These displayed polypeptides comprise at least one unnatural amino acid, e.g., an aryl-azide amino acid such as p-azido-L-phenylalanine, or an alkynyl-amino acid such as para-propargyloxyphenylalanine, which are incorporated into the phage-displayed fusion polypeptide at a selected position by using an in vivo orthogonal translation system comprising a suitable orthogonal aminoacyl-tRNA synthetase and a suitable orthogonal tRNA species. These unnatural amino acids advantageously provide targets for posttranslational modifications such as azide-alkyne [3+2]cycloaddition reactions and Staudinger modifications.

Inventors:
; ;
Issue Date:
Research Org.:
The Scripps Research Institute (La Jolla, CA)
Sponsoring Org.:
USDOE
OSTI Identifier:
1083220
Patent Number(s):
8,367,588
Application Number:
11/992,341
Assignee:
The Scripps Research Institute (La Jolla, CA)
Resource Type:
Patent
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES

Citation Formats

Tsao, Meng-Lin, Tian, Feng, and Schultz, Peter. Selective posttranslational modification of phage-displayed polypeptides. United States: N. p., 2013. Web.
Tsao, Meng-Lin, Tian, Feng, & Schultz, Peter. Selective posttranslational modification of phage-displayed polypeptides. United States.
Tsao, Meng-Lin, Tian, Feng, and Schultz, Peter. Tue . "Selective posttranslational modification of phage-displayed polypeptides". United States. https://www.osti.gov/servlets/purl/1083220.
@article{osti_1083220,
title = {Selective posttranslational modification of phage-displayed polypeptides},
author = {Tsao, Meng-Lin and Tian, Feng and Schultz, Peter},
abstractNote = {The invention relates to posttranslational modification of phage-displayed polypeptides. These displayed polypeptides comprise at least one unnatural amino acid, e.g., an aryl-azide amino acid such as p-azido-L-phenylalanine, or an alkynyl-amino acid such as para-propargyloxyphenylalanine, which are incorporated into the phage-displayed fusion polypeptide at a selected position by using an in vivo orthogonal translation system comprising a suitable orthogonal aminoacyl-tRNA synthetase and a suitable orthogonal tRNA species. These unnatural amino acids advantageously provide targets for posttranslational modifications such as azide-alkyne [3+2]cycloaddition reactions and Staudinger modifications.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {2013},
month = {2}
}

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