Genetically programmed expression of proteins containing the unnatural amino acid phenylselenocysteine

Wang, Jiangyun; Schultz, Peter G.

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Title: Genetically programmed expression of proteins containing the unnatural amino acid phenylselenocysteine

Abstract

The invention relates to orthogonal pairs of tRNAs and aminoacyl-tRNA synthetase that can incorporate the unnatural amino acid phenylselenocysteine into proteins produced in eubacterial host cells such as E. coli. The invention provides, for example but not limited to, novel orthogonal aminoacyl-tRNA synthetases, polynucleotides encoding the novel sythetases molecules, methods for identifying and making the novel synthetases, methods for producing containing the unnatural amino acid phenylselenocysteine and translation systems. The invention further provides methods for producing modified proteins (e.g., lapidated proteins) through targeted modification of the phenylselenocysteine residue in a protein.

Inventors:: Wang, Jiangyun; Schultz, Peter G.

Issue Date:: Tue Mar 12 00:00:00 EDT 2013

Research Org.:: DOESC (USDOE Office of Science (SC) (United States))

Sponsoring Org.:: USDOE

OSTI Identifier:: 1083058

Patent Number(s):: 8394605

Application Number:: 13/492,240

Assignee:: The Scripps Research Institute (La Jolla, CA)

Patent Classifications (CPCs):: C - CHEMISTRY C12 - BIOCHEMISTRY C12N - MICROORGANISMS OR ENZYMES

C - CHEMISTRY C12 - BIOCHEMISTRY C12P - FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE {

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C - CHEMISTRY C12 - BIOCHEMISTRY C12N - MICROORGANISMS OR ENZYMES
C12N15/67 - General methods for enhancing the expression
C12N9/104 - {Aminoacyltransferases
C12N9/93 - {Ligases

C - CHEMISTRY C12 - BIOCHEMISTRY C12P - FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE {
C12P21/02 - having a known sequence of two or more amino acids, e.g. glutathione

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DOE Contract Number:: ER46051; ER45812

Resource Type:: Patent

Country of Publication:: United States

Language:: English

Subject:: 59 BASIC BIOLOGICAL SCIENCES

Citation Formats


                    Wang, Jiangyun, and Schultz, Peter G. Genetically programmed expression of proteins containing the unnatural amino acid phenylselenocysteine.  United States: N. p., 2013. 
        Web.

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                    Wang, Jiangyun, & Schultz, Peter G. Genetically programmed expression of proteins containing the unnatural amino acid phenylselenocysteine.  United States.

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                    Wang, Jiangyun, and Schultz, Peter G. Tue .  
        "Genetically programmed expression of proteins containing the unnatural amino acid phenylselenocysteine".  United States.  https://www.osti.gov/servlets/purl/1083058.

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@article{osti_1083058,

  title        = {Genetically programmed expression of proteins containing the unnatural amino acid phenylselenocysteine},

  author       = {Wang, Jiangyun and Schultz, Peter G.},

  abstractNote = {The invention relates to orthogonal pairs of tRNAs and aminoacyl-tRNA synthetase that can incorporate the unnatural amino acid phenylselenocysteine into proteins produced in eubacterial host cells such as E. coli. The invention provides, for example but not limited to, novel orthogonal aminoacyl-tRNA synthetases, polynucleotides encoding the novel sythetases molecules, methods for identifying and making the novel synthetases, methods for producing containing the unnatural amino acid phenylselenocysteine and translation systems. The invention further provides methods for producing modified proteins (e.g., lapidated proteins) through targeted modification of the phenylselenocysteine residue in a protein.},

  doi          = {},

  journal      = {},
number       = ,

  volume       = ,

  place        = {United States},

  year         = {Tue Mar 12 00:00:00 EDT 2013},

  month        = {Tue Mar 12 00:00:00 EDT 2013}

}

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Works referenced in this record:

Glycoprotein synthesis
patent-application, July 2007

Schultz, Peter; Wang, Lei; Zhang, Zhiwen
US Patent Application 11/580203; 20070172915
URL: https://image-ppubs.uspto.gov/dirsearch-public/print/downloadPdf/20070172915

A chemical toolkit for proteins — an expanded genetic code
journal, August 2006

Xie, Jianming; Schultz, Peter G.
Nature Reviews Molecular Cell Biology, Vol. 7, Issue 10
https://doi.org/10.1038/nrm2005

Unnatural reactive amino acid genetic code additions
patent-application, December 2004

Deiters, Alexander; Cropp, T. Ashton; Chin, Jason W.
US Patent Application 10/826919; 20040265952
URL: https://image-ppubs.uspto.gov/dirsearch-public/print/downloadPdf/20040265952

Convergent Synthesis of Peptide Conjugates Using Dehydroalanines for Chemoselective Ligations
journal, March 2001

Zhu, Yantao; van der Donk, Wilfred A.
Organic Letters, Vol. 3, Issue 8
https://doi.org/10.1021/ol015648a

Site specific incorporation of keto amino acids into proteins
patent-application, March 2006

Schultz, Peter G.; Wang, Lei
US Patent Application 10/530421; 20060068478
URL: https://image-ppubs.uspto.gov/dirsearch-public/print/downloadPdf/20060068478

Glycoprotein synthesis
patent, February 2007

Schultz, Peter G.; Wang, Lei; Zhang, Zhiwen
US Patent Document 7,183,082
URL: https://image-ppubs.uspto.gov/dirsearch-public/print/downloadPdf/7183082

Selective incorporation of 5-hydroxytryptophan into proteins in mammalian cells
patent-application, May 2007

Zhang, Zhiwen; Alfonta, Lital; Schultz, Peter
US Patent Application 10/580987; 20070111193
URL: https://image-ppubs.uspto.gov/dirsearch-public/print/downloadPdf/20070111193

Expanding the eukaryotic genetic code
patent-application, January 2005

Chin, Jason W.; Cropp, T. Ashton; Anderson, J. Christopher
US Patent Application 10/825867; 20050009049
URL: https://image-ppubs.uspto.gov/dirsearch-public/print/downloadPdf/20050009049

An unnatural base pair for incorporating amino acid analogs into proteins
journal, February 2002

Hirao, Ichiro; Ohtsuki, Takashi; Fujiwara, Tsuyoshi
Nature Biotechnology, Vol. 20, Issue 2
https://doi.org/10.1038/nbt0202-177

Prediction of protein function from protein sequence and structure
journal, January 1999

Whisstock, James C.; Lesk, Arthur M.
Quarterly Reviews of Biophysics, Vol. 36, Issue 3, p. 307-340
https://doi.org/10.1017/S0033583503003901

Adding amino acids to the genetic repertoire
journal, December 2005

Xie, Jianming; Schultz, Peter G.
Current Opinion in Chemical Biology, Vol. 9, Issue 6, p. 548-554
https://doi.org/10.1016/j.cbpa.2005.10.011

Methods of Incorporating Amino Acid Analogs into Proteins
patent-application, December 2005

Kwon, Inchan; Tirrell, David
US Patent Application 11/130583; 20050287639
URL: https://image-ppubs.uspto.gov/dirsearch-public/print/downloadPdf/20050287639

Compositions of orthogonal leucyl-trna and aminoacyl-trna synthetase pairs and uses thereof
patent-application, July 2006

Anderson, J. Christopher; Schultz, Peter G.
US Patent Application 10/563655; 20060160175
URL: https://image-ppubs.uspto.gov/dirsearch-public/print/downloadPdf/20060160175

Site-specific labeling of proteins for NMR studies
patent-application, May 2006

Deiters, Alexander; Geierstanger, Bernhard H.; Schultz, Peter G.
US Patent Application 11/233466; 20060110784
URL: https://image-ppubs.uspto.gov/dirsearch-public/print/downloadPdf/20060110784

Site-specific incorporation of redox active amino acids into proteins
patent-application, October 2005

Alfonta, Lital; Schultz, Peter G.; Zhang, Zhiwen
US Patent Application 10/965218; 20050227318
URL: https://image-ppubs.uspto.gov/dirsearch-public/print/downloadPdf/20050227318

Selective posttranslational modification of phage-displayed polypeptides
patent-application, August 2007

Tsao, Meng-Lin; Tian, Feng; Schultz, Peter
US Patent Application 11/580223; 20070178448
URL: https://image-ppubs.uspto.gov/dirsearch-public/print/downloadPdf/20070178448

From genes to protein structure and function: novel applications of computational approaches in the genomic era
journal, January 2000

Skolnick, Jeffrey; Fetrow, Jacquelyn S.
Trends in Biotechnology, Vol. 18, Issue 1, p. 34-39
https://doi.org/10.1016/S0167-7799(99)01398-0

Glycoprotein synthesis
patent, April 2007

Shultz, Peter G.; Wang, Lei; Zhang, Zhiwen
US Patent Document 7,199,222
URL: https://image-ppubs.uspto.gov/dirsearch-public/print/downloadPdf/7199222

Semisynthetic production of unnatural L-α-amino acids by metabolic engineering of the cysteine-biosynthetic pathway
journal, March 2003

Maier, Thomas H. P.
Nature Biotechnology, Vol. 21, Issue 4
https://doi.org/10.1038/nbt807

Site specific incorporation of heavy atom-containing unnatural amino acids into proteins for structure determination
patent-application, December 2005

Xie, Jianming; Wang, Lei; Wu, Ning
US Patent Application 11/137850; 20050272121
URL: https://image-ppubs.uspto.gov/dirsearch-public/print/downloadPdf/20050272121

Site-specific incorporation of redox active amino acids into proteins
patent-application, January 2007

Alfonta, Lital; Schultz, Peter G.; Zhang, Zhiwen
US Patent Application 10/575991; 20070009990
URL: https://image-ppubs.uspto.gov/dirsearch-public/print/downloadPdf/20070009990

Expanding the eukaryotic genetic code
patent-application, July 2007

Chin, Jason; Cropp, T. Ashton; Anderson, J. Christopher
US Patent Application 11/710672; 20070166791
URL: https://image-ppubs.uspto.gov/dirsearch-public/print/downloadPdf/20070166791

Compositions of orthogonal lysyl-trna and aminoacyl-trna synthetase pairs and uses thereof
patent-application, February 2007

Anderson, J. Christopher; Wu, Ning; Santoro, Stephen
US Patent Application 10/563686; 20070042461
URL: https://image-ppubs.uspto.gov/dirsearch-public/print/downloadPdf/20070042461

A Biosynthetic Route to Dehydroalanine-Containing Proteins
journal, September 2007

Wang, Jiangyun; Schiller, Stefan M.; Schultz, Peter G.
Angewandte Chemie International Edition, Vol. 46, Issue 36
https://doi.org/10.1002/anie.200702305

Methods and composition for the production of orthogonal tRNA-aminoacyltRNA synthetase pairs
patent-application, October 2006

Schultz, Peter; Wang, Lei; Anderson, John Christopher
US Patent Application 11/254170; 20060234367
URL: https://image-ppubs.uspto.gov/dirsearch-public/print/downloadPdf/20060234367

In vivo incorporation of alkynyl amino acids into proteins in eubacteria
patent-application, April 2006

Deiters, Alexander; Schultz, Peter
US Patent Application 11/232425; 20060073507
URL: https://image-ppubs.uspto.gov/dirsearch-public/print/downloadPdf/20060073507

patent-application, October 2004

Schultz, Peter G.; Wang, Lei
US Patent Application 10/744899; 20040198637
URL: https://image-ppubs.uspto.gov/dirsearch-public/print/downloadPdf/20040198637

Expanding tRNA recognition of a tRNA synthetase by a single amino acid change
journal, May 2003

Feng, L.; Tumbula-Hansen, D.; Toogood, H.
Proceedings of the National Academy of Sciences, Vol. 100, Issue 10, p. 5676-5681
https://doi.org/10.1073/pnas.0631525100

Methods and composition for the production of orthogonal tRNA-aminoacyltRNA synthetase pairs
patent-application, March 2006

Schultz, Peter; Wang, Lei; Anderson, John Christopher
US Patent Application 11/002387; 20060063244
URL: https://image-ppubs.uspto.gov/dirsearch-public/print/downloadPdf/20060063244

Enzymatic Phosphorylation of Unnatural Nucleosides
journal, December 2002

Wu, Yiqin; Fa, Ming; Tae, Eunju Lee
Journal of the American Chemical Society, Vol. 124, Issue 49
https://doi.org/10.1021/ja028050m

Production and fluorescence-activated cell sorting of Escherichia coli expressing a functional antibody fragment on the external surface.
journal, November 1993

Francisco, J. A.; Campbell, R.; Iverson, B. L.
Proceedings of the National Academy of Sciences, Vol. 90, Issue 22
https://doi.org/10.1073/pnas.90.22.10444

Progress toward the evolution of an organism with an expanded genetic code
journal, April 1999

Liu, David R.; Schultz, Peter G.
Proceedings of the National Academy of Sciences, Vol. 96, Issue 9, p. 4780-4785
https://doi.org/10.1073/pnas.96.9.4780

An expanding genetic code
journal, July 2005

Xie, Jianming; Schultz, Peter G.
Methods, Vol. 36, Issue 3, p. 227-238
https://doi.org/10.1016/j.ymeth.2005.04.010

A "Silent" Polymorphism in the MDR1 Gene Changes Substrate Specificity
journal, January 2007

Kimchi-Sarfaty, C.; Oh, J. M.; Kim, I.-W.
Science, Vol. 315, Issue 5811, p. 525-528
https://doi.org/10.1126/science.1135308

Glycoprotein synthesis
patent, August 2005

Schultz, Peter G.; Wang, Lei; Zhang, Zhiwen
US Patent Document 6,927,042
URL: https://image-ppubs.uspto.gov/dirsearch-public/print/downloadPdf/6927042

Compositions of orthogonal lysyl-tRNA and aminoacyl-tRNA synthetase pairs and uses thereof
patent-application, August 2006

Anderson, J. Christopher; Wu, Ning; Santoro, Stephen
US Patent Application 11/326970; 20060177900
URL: https://image-ppubs.uspto.gov/dirsearch-public/print/downloadPdf/20060177900

Expanding the eukaryotic genetic code
patent-application, July 2007

Chin, Jason W.; Cropp, T. Ashton; Anderson, J. Christopher
US Patent Application 11/710655; 20070154952
URL: https://image-ppubs.uspto.gov/dirsearch-public/print/downloadPdf/20070154952

Methods and composition for the production of orthogonal tRNA-aminoacyltRNA synthetase pairs
patent-application, September 2005

Schultz, Peter; Wang, Lei; Anderson, John Christopher
US Patent Application 11/009635; 20050208536
URL: https://image-ppubs.uspto.gov/dirsearch-public/print/downloadPdf/20050208536

Glycoprotein synthesis
patent, May 2007

Schultz, Peter G.; Wang, Lei; Zhang, Zhiwen
US Patent Document 7,217,809
URL: https://image-ppubs.uspto.gov/dirsearch-public/print/downloadPdf/7217809

Methods and compositions for the production of orthogonal tRNA-aminoacyl tRNA synthetase pairs
patent, August 2006

Schultz, Peter G.; Wang, Lei; Anderson, John Christopher
US Patent Document 7,083,970
URL: https://image-ppubs.uspto.gov/dirsearch-public/print/downloadPdf/7083970

Glycoprotein synthesis
patent-application, August 2007

Schultz, Peter; Wang, Lei; Zhang, Zhiwen
US Patent Application 11/520849; 20070184517
URL: https://image-ppubs.uspto.gov/dirsearch-public/print/downloadPdf/20070184517

Glycoprotein synthesis
patent, July 2007

Schultz, Peter G.; Wang, Lei; Zhang, Zhiwen
US Patent Document 7,238,510
URL: https://image-ppubs.uspto.gov/dirsearch-public/print/downloadPdf/7238510

Adding photoregulated amino acids to the genetic code
patent-application, June 2006

Deiters, Alexander; Wu, Ning; Schultz, Peter G.
US Patent Application 11/233508; 20060134746
URL: https://image-ppubs.uspto.gov/dirsearch-public/print/downloadPdf/20060134746

Glycoprotein synthesis
patent, October 2006

Schultz, Peter G.; Wang, Lei; Zhang, Zhiwen
US Patent Document 7,129,333
URL: https://image-ppubs.uspto.gov/dirsearch-public/print/downloadPdf/7129333

Mutational identification of an essential tryptophan in tryptophanyl-tRNA synthetase of Bacillus subtilis.
journal, May 1992

Chow, K. C.; Xue, H.; Shi, W.
Journal of Biological Chemistry, Vol. 267, Issue 13
https://doi.org/10.1016/S0021-9258(19)50401-X

Methods and compositions for the production of orthogonal tRNA-aminoacyltRNA synthetase pairs
patent-application, June 2003

Schultz, Peter; Wang, Lei; Anderson, John Christopher
US Patent Application 10/126931; 20030108885
URL: https://image-ppubs.uspto.gov/dirsearch-public/print/downloadPdf/20030108885

Composition of orthogonal glutamyl-trna and aminoacyl-trna synthetase pairs and uses thereof
patent-application, January 2007

Anderson, J. Christopher; Schultz, Peter G.; Santoro, Stephen
US Patent Application 10/563656; 20070020634
URL: https://image-ppubs.uspto.gov/dirsearch-public/print/downloadPdf/20070020634

Glycoprotein synthesis
patent, August 2007

Schultz, Peter G.; Wang, Lei; Zhang, Zhiwen
US Patent Document 7,262,040
URL: https://image-ppubs.uspto.gov/dirsearch-public/print/downloadPdf/7262040

Selective incorporation of 5-hydroxytryptophan into proteins in mammalian cells
patent-application, June 2005

Zhang, Zhiwen; Alfonta, Lital; Schultz, Peter
US Patent Application 11/016348; 20050136513
URL: https://image-ppubs.uspto.gov/dirsearch-public/print/downloadPdf/20050136513

Expanding the genetic code
journal, December 2001

Wang, Lei; Schultz, Peter G.
Chemical Communications, Issue 1
https://doi.org/10.1039/b108185n

In vivo incorporation of unnatural amino acids
patent, May 2006

Schultz, Peter G.; Wang, Lei; Anderson, John Christopher
US Patent Document 7,045,337
URL: https://image-ppubs.uspto.gov/dirsearch-public/print/downloadPdf/7045337