High density growth of T7 expression strains with auto-induction option

Studier, F. William

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Title: High density growth of T7 expression strains with auto-induction option

Abstract

A method for promoting and suppressing auto-induction of transcription of a cloned gene 1 of bacteriophage T7 in cultures of bacterial cells grown batchwise is disclosed. The transcription is under the control of a promoter whose activity can be induced by an exogenous inducer whose ability to induce said promoter is dependent on the metabolic state of said bacterial cells.

Inventors:: Studier, F. William

Issue Date:: Tue Mar 19 00:00:00 EDT 2013

Research Org.:: Brookhaven National Laboratory (BNL), Upton, NY (United States)

Sponsoring Org.:: USDOE

OSTI Identifier:: 1083033

Patent Number(s):: 8399217

Application Number:: 11/741,282

Assignee:: Brookhaven Science Associates, LLC (Upton, NY)

Patent Classifications (CPCs):: C - CHEMISTRY C12 - BIOCHEMISTRY C12N - MICROORGANISMS OR ENZYMES

C - CHEMISTRY C12 - BIOCHEMISTRY C12P - FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE {

Show more

C - CHEMISTRY C12 - BIOCHEMISTRY C12N - MICROORGANISMS OR ENZYMES
C12N1/20 - Bacteria {
C12N15/72 - Expression systems using regulatory sequences derived from the lac-operon

C - CHEMISTRY C12 - BIOCHEMISTRY C12P - FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE {
C12P19/34 - Polynucleotides, e.g. nucleic acids, oligoribonucleotides

Show less

DOE Contract Number:: AC02-98CH10886

Resource Type:: Patent

Country of Publication:: United States

Language:: English

Subject:: 59 BASIC BIOLOGICAL SCIENCES

Citation Formats


                    Studier, F. William. High density growth of T7 expression strains with auto-induction option.  United States: N. p., 2013. 
        Web.

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                    Studier, F. William. High density growth of T7 expression strains with auto-induction option.  United States.

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                    Studier, F. William. Tue .  
        "High density growth of T7 expression strains with auto-induction option".  United States.  https://www.osti.gov/servlets/purl/1083033.

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@article{osti_1083033,

  title        = {High density growth of T7 expression strains with auto-induction option},

  author       = {Studier, F. William},

  abstractNote = {A method for promoting and suppressing auto-induction of transcription of a cloned gene 1 of bacteriophage T7 in cultures of bacterial cells grown batchwise is disclosed. The transcription is under the control of a promoter whose activity can be induced by an exogenous inducer whose ability to induce said promoter is dependent on the metabolic state of said bacterial cells.},

  doi          = {},

  journal      = {},
number       = ,

  volume       = ,

  place        = {United States},

  year         = {Tue Mar 19 00:00:00 EDT 2013},

  month        = {Tue Mar 19 00:00:00 EDT 2013}

}

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Works referenced in this record:

Spontaneous cAMP-dependent derepression of gene expression in stationary phase plays a role in recombinant expression instability
journal, March 1998

Grossman, Trudy H.; Kawasaki, Ernest S.; Punreddy, Sandhya R.
Gene, Vol. 209, Issue 1-2
https://doi.org/10.1016/S0378-1119(98)00020-1

Novel escherichia coli strain allows efficient recombinant protein production using lactose as inducer
journal, June 2003

Menzella, Hugo G.; Ceccarelli, Eduardo A.; Gramajo, Hugo C.
Biotechnology and Bioengineering, Vol. 82, Issue 7, p. 809-817
https://doi.org/10.1002/bit.10630

Enhanced Bacterial Protein Expression During Auto-Induction Obtained by Alteration of Lac Repressor Dosage and Medium Composition
journal, January 2007

Blommel, Paul G.; Becker, Katie J.; Duvnjak, Petar
Biotechnology Progress, Vol. 23, Issue 3
https://doi.org/10.1021/bp070011x

Controlling basal expression in an inducible T7 expression system by blocking the target T7 promoter with lac repressor
journal, May 1991

Dubendorf, John W.; Studier, F. William
Journal of Molecular Biology, Vol. 219, Issue 1
https://doi.org/10.1016/0022-2836(91)90856-2

Maximizing the expression of a recombinant gene in Escherichia coli by manipulation of induction time using lactose as inducer
journal, March 1992

Neubauer, P.; Hofmann, K.; Holst, O.
Applied Microbiology and Biotechnology, Vol. 36, Issue 6
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Recombinant protein expression and solubility screening in Escherichia coli : a comparative study
journal, September 2006

Berrow, Nick S.; Büssow, K.; Coutard, B.
Acta Crystallographica Section D Biological Crystallography, Vol. 62, Issue 10
https://doi.org/10.1107/S0907444906031337

The Overnight Express Autoinduction System: High-density cell growth and protein expression while you sleep
journal, March 2005

Grabski, Anthony; Mehler, Mark; Drott, Don
Nature Methods, Vol. 2, Issue 3
https://doi.org/10.1038/nmeth0305-233

Protein production in Escherichia coli for structural studies by X-ray crystallography
journal, April 2003

Goulding, Celia W.; Jeanne Perry, L.
Journal of Structural Biology, Vol. 142, Issue 1
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cAMP receptor protein-cAMP plays a crucial role in glucose-lactose diauxie by activating the major glucose transporter gene in Escherichia coli
journal, November 1997

Kimata, K.; Takahashi, H.; Inada, T.
Proceedings of the National Academy of Sciences, Vol. 94, Issue 24
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The General Stress Sigma Factor ς ^S of Escherichia coli Is Induced during Diauxic Shift from Glucose to Lactose
journal, December 1998

Fischer, Daniela; Teich, Antje; Neubauer, Peter
Journal of Bacteriology, Vol. 180, Issue 23
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Lactose Fed-Batch Overexpression of Recombinant Metalloproteins in Escherichia coli BL21(DE3): Process Control Yielding High Levels of Metal-Incorporated, Soluble Protein
journal, October 1995

Hoffman, B. J.; Broadwater, J. A.; Johnson, P.
Protein Expression and Purification, Vol. 6, Issue 5
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Auto-induction medium for the production of [U-15N]- and [U-13C, U-15N]-labeled proteins for NMR screening and structure determination
journal, April 2005

Tyler, Robert C.; Sreenath, Hassan K.; Singh, Shanteri
Protein Expression and Purification, Vol. 40, Issue 2
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Protein production by auto-induction in high-density shaking cultures
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Studier, F. William
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Use of bacteriophage T7 RNA polymerase to direct selective high-level expression of cloned genes
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Studier, F. William; Moffatt, Barbara A.
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[6] Use of T7 RNA polymerase to direct expression of cloned genes
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Expression, purification and crystallization of native and selenomethionine labeled Mycobacterium tuberculosis FGD1 (Rv0407) using a Mycobacterium smegmatis expression system
journal, July 2007

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Protocols for production of selenomethionine-labeled proteins in 2-L polyethylene terephthalate bottles using auto-induction medium
journal, April 2005

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High density growth of T7 expression strains with auto-induction option

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Disclosed is a method for promoting auto-induction of transcription of cloned DNA in cultures of bacterial cells grown batchwise, the transcription being under the control of a promoter whose activity can be induced by an exogenous inducer whose ability to induce said promoter is dependent on the metabolic state of said bacterial cells. Initially, a culture media is provided which includes: i) an inducer that causes induction of transcription from said promoter in said bacterial cells; and ii) a metabolite that prevents induction by said inducer, the concentration of said metabolite being adjusted so as to substantially preclude induction bymore » « less
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Similar Records

Title: High density growth of T7 expression strains with auto-induction option

Abstract

Citation Formats

Spontaneous cAMP-dependent derepression of gene expression in stationary phase plays a role in recombinant expression instability journal, March 1998

Novel escherichia coli strain allows efficient recombinant protein production using lactose as inducer journal, June 2003

Enhanced Bacterial Protein Expression During Auto-Induction Obtained by Alteration of Lac Repressor Dosage and Medium Composition journal, January 2007

Controlling basal expression in an inducible T7 expression system by blocking the target T7 promoter with lac repressor journal, May 1991

Maximizing the expression of a recombinant gene in Escherichia coli by manipulation of induction time using lactose as inducer journal, March 1992

Recombinant protein expression and solubility screening in Escherichia coli : a comparative study journal, September 2006

The Overnight Express Autoinduction System: High-density cell growth and protein expression while you sleep journal, March 2005

Protein production in Escherichia coli for structural studies by X-ray crystallography journal, April 2003

cAMP receptor protein-cAMP plays a crucial role in glucose-lactose diauxie by activating the major glucose transporter gene in Escherichia coli journal, November 1997

The General Stress Sigma Factor ς S of Escherichia coli Is Induced during Diauxic Shift from Glucose to Lactose journal, December 1998

Lactose Fed-Batch Overexpression of Recombinant Metalloproteins in Escherichia coli BL21(DE3): Process Control Yielding High Levels of Metal-Incorporated, Soluble Protein journal, October 1995

Auto-induction medium for the production of [U-15N]- and [U-13C, U-15N]-labeled proteins for NMR screening and structure determination journal, April 2005

Protein production by auto-induction in high-density shaking cultures journal, May 2005

Use of bacteriophage T7 RNA polymerase to direct selective high-level expression of cloned genes journal, May 1986

[6] Use of T7 RNA polymerase to direct expression of cloned genes book, January 1990

Fermentation conditions for high-level expression of the tac -promoter-controlled calf prochymosin cDNA in Escherichia coli HB101: FERMENTATION CONDITIONS FOR HIGH-LEVEL EXPRESSION journal, January 1991

Heterologous expression of proteins from Plasmodium falciparum: Results from 1000 genes journal, August 2006

Expression, purification and crystallization of native and selenomethionine labeled Mycobacterium tuberculosis FGD1 (Rv0407) using a Mycobacterium smegmatis expression system journal, July 2007

Protocols for production of selenomethionine-labeled proteins in 2-L polyethylene terephthalate bottles using auto-induction medium journal, April 2005

Spontaneous cAMP-dependent derepression of gene expression in stationary phase plays a role in recombinant expression instability
journal, March 1998

Novel escherichia coli strain allows efficient recombinant protein production using lactose as inducer
journal, June 2003

Enhanced Bacterial Protein Expression During Auto-Induction Obtained by Alteration of Lac Repressor Dosage and Medium Composition
journal, January 2007

Controlling basal expression in an inducible T7 expression system by blocking the target T7 promoter with lac repressor
journal, May 1991

Maximizing the expression of a recombinant gene in Escherichia coli by manipulation of induction time using lactose as inducer
journal, March 1992

Recombinant protein expression and solubility screening in Escherichia coli : a comparative study
journal, September 2006

The Overnight Express Autoinduction System: High-density cell growth and protein expression while you sleep
journal, March 2005

Protein production in Escherichia coli for structural studies by X-ray crystallography
journal, April 2003

cAMP receptor protein-cAMP plays a crucial role in glucose-lactose diauxie by activating the major glucose transporter gene in Escherichia coli
journal, November 1997

The General Stress Sigma Factor ς ^S of Escherichia coli Is Induced during Diauxic Shift from Glucose to Lactose
journal, December 1998

Lactose Fed-Batch Overexpression of Recombinant Metalloproteins in Escherichia coli BL21(DE3): Process Control Yielding High Levels of Metal-Incorporated, Soluble Protein
journal, October 1995

Auto-induction medium for the production of [U-15N]- and [U-13C, U-15N]-labeled proteins for NMR screening and structure determination
journal, April 2005

Protein production by auto-induction in high-density shaking cultures
journal, May 2005

Use of bacteriophage T7 RNA polymerase to direct selective high-level expression of cloned genes
journal, May 1986

[6] Use of T7 RNA polymerase to direct expression of cloned genes
book, January 1990

Fermentation conditions for high-level expression of the tac -promoter-controlled calf prochymosin cDNA in Escherichia coli HB101: FERMENTATION CONDITIONS FOR HIGH-LEVEL EXPRESSION
journal, January 1991

Heterologous expression of proteins from Plasmodium falciparum: Results from 1000 genes
journal, August 2006

Expression, purification and crystallization of native and selenomethionine labeled Mycobacterium tuberculosis FGD1 (Rv0407) using a Mycobacterium smegmatis expression system
journal, July 2007

Protocols for production of selenomethionine-labeled proteins in 2-L polyethylene terephthalate bottles using auto-induction medium
journal, April 2005