Method and apparatus for sustaining viability of biological cells on a substrate

McKnight, Timothy E.; Melechko, Anatoli V.; Simpson, Michael L.

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Title: Method and apparatus for sustaining viability of biological cells on a substrate

Abstract

A method for the transient transformation of a living biological cell having an intact cell membrane defining an intracellular domain, and an apparatus for the transient transformation of biological cells. The method and apparatus include introducing a compartmentalized extracellular component fixedly attached to a cellular penetrant structure to the intracellular domain of the cell, wherein the cell is fixed in a predetermined location and wherein the component is expressed within in the cell while being retained within the compartment and wherein the compartment restricts the mobility and interactions of the component within the cell and prevents transference of the component to the cell.

Inventors:: McKnight, Timothy E.; Melechko, Anatoli V.; Simpson, Michael L.

Issue Date:: Tue Jan 01 00:00:00 EST 2013

Research Org.:: ORO (Oak Ridge Office, Oak Ridge, TN (United States))

Sponsoring Org.:: USDOE

OSTI Identifier:: 1082935

Patent Number(s):: 8343766

Application Number:: 13/305,134

Assignee:: UT-Battle, LLC (Oak Ridge, TN)

Patent Classifications (CPCs):: B - PERFORMING OPERATIONS B01 - PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL B01L - CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE

Y - NEW / CROSS SECTIONAL TECHNOLOGIES Y10 - TECHNICAL SUBJECTS COVERED BY FORMER USPC Y10S - TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS

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B - PERFORMING OPERATIONS B01 - PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL B01L - CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
B01L3/02 - Burettes

Y - NEW / CROSS SECTIONAL TECHNOLOGIES Y10 - TECHNICAL SUBJECTS COVERED BY FORMER USPC Y10S - TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
Y10S977/876 - Nanotube tip

Show less

DOE Contract Number:: AC05-00OR22725

Resource Type:: Patent

Country of Publication:: United States

Language:: English

Subject:: 60 APPLIED LIFE SCIENCES

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                    McKnight, Timothy E., Melechko, Anatoli V., and Simpson, Michael L. Method and apparatus for sustaining viability of biological cells on a substrate.  United States: N. p., 2013. 
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                    McKnight, Timothy E., Melechko, Anatoli V., & Simpson, Michael L. Method and apparatus for sustaining viability of biological cells on a substrate.  United States.

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                    McKnight, Timothy E., Melechko, Anatoli V., and Simpson, Michael L. Tue .  
        "Method and apparatus for sustaining viability of biological cells on a substrate".  United States.  https://www.osti.gov/servlets/purl/1082935.

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@article{osti_1082935,

  title        = {Method and apparatus for sustaining viability of biological cells on a substrate},

  author       = {McKnight, Timothy E. and Melechko, Anatoli V. and Simpson, Michael L.},

  abstractNote = {A method for the transient transformation of a living biological cell having an intact cell membrane defining an intracellular domain, and an apparatus for the transient transformation of biological cells. The method and apparatus include introducing a compartmentalized extracellular component fixedly attached to a cellular penetrant structure to the intracellular domain of the cell, wherein the cell is fixed in a predetermined location and wherein the component is expressed within in the cell while being retained within the compartment and wherein the compartment restricts the mobility and interactions of the component within the cell and prevents transference of the component to the cell.},

  doi          = {},

  journal      = {},
number       = ,

  volume       = ,

  place        = {United States},

  year         = {Tue Jan 01 00:00:00 EST 2013},

  month        = {Tue Jan 01 00:00:00 EST 2013}

}

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Similar Records

Title: Method and apparatus for sustaining viability of biological cells on a substrate

Abstract

Citation Formats

Direct haplotyping of kilobase-size DNA using carbon nanotube probes journal, July 2000

Fabrication and Characterization of Vertically Aligned Carbon Nanotubes on Silicon Substrates Using Porous Alumina Nanotemplates journal, April 2002

Effects of spatial separation on the growth of vertically aligned carbon nanofibers produced by plasma-enhanced chemical vapor deposition journal, January 2002

Carbon nanotube array-based biosensor journal, January 2003

Major limitations in the use of cationic liposomes for DNA delivery journal, March 1998

Nanoneedle chips and the production thereof patent-application, April 2004

DNA-bridged carbon nanotube arrays patent, October 2005

Microarrays of cells expressing defined cDNAs journal, May 2001

Block and Graft Copolymers and Nanogel™ Copolymer Networks for DNA Delivery into Cell journal, January 2000

The use of Chinese hamster ovary cells to quantify specific locus mutation and to determine mutagenicity of chemicals: A report of the GENE-TOX program journal, March 1981

Micromachined interfaces: new approaches in cell immunoisolation and biomolecular separation journal, October 2000

In Vivo Imaging of Quantum Dots Encapsulated in Phospholipid Micelles journal, November 2002

Construction of a genetic toggle switch in Escherichia coli journal, January 2000

Tracking Gene Expression after DNA Delivery Using Spatially Indexed Nanofiber Arrays journal, July 2004

Field-emission studies of smooth and nanostructured carbon films journal, August 1999

Rapid synthesis of carbon nanotubes and carbon encapsulated metal nanoparticles by a displacement reaction patent, November 2002

Cell-manipulating apparatus and method of using the same patent-application, December 2003

Gene expression using an ultrathin needle enabling accurate displacement and low invasiveness journal, July 2005

Fabrication of Poly(ethylene glycol) Hydrogel Microstructures Using Photolithography journal, September 2001

Covalently functionalized nanotubes as nanometre- sized probes in chemistry and biology journal, July 1998

Nanoparticle delivery system patent-application, April 2004

Nanotransfer and nanoreplication using deterministically grown sacrificial nanotemplates patent-application, August 2005

Parallel macromolecular delivery and biochemical/electrochemical interface to cells employing nanostructures patent-application, October 2004

Microfabricated Microneedles for Gene and Drug Delivery journal, August 2000

Synthesis of Large Arrays of Well-Aligned Carbon Nanotubes on Glass journal, November 1998

Microprojection array immunization patch and method patent-application, December 2002

Nanotube Nanotweezers journal, December 1999

Combinatorial Synthesis of Genetic Networks journal, May 2002

Temporally resolved catecholamine spikes correspond to single vesicle release from individual chromaffin cells. journal, December 1991

Remote electronic control of DNA hybridization through inductive coupling to an attached metal nanocrystal antenna journal, January 2002

A galinstan expansion femtosyringe for microinjection of eukaryotic organelles and prokaryotes journal, September 1999

Activation of the NADPH oxidase in human fibroblasts by mechanical intrusion of a single cell with an ultramicroelectrode journal, March 1997

Gated fabrication of nanostructure field emission cathode material within a device patent-application, November 2008

Structural and functional imaging with carbon nanotube AFM probes journal, January 2001

Laterally Stabilized Complexes of DNA with Linear Reducible Polycations: Strategy for Triggered Intracellular Activation of DNA Delivery Vectors journal, January 2002

Electrochemically induced adsorption of radio-labeled DNA on gold and HOPG substrates for STM investigations journal, December 1991

The emerging field of nanotube biotechnology journal, January 2003

DNA-functionalized single-walled carbon nanotubes journal, September 2002

Modelling cellular behaviour journal, January 2001

Carbon nanotube-containing catalysts, methods of making, and reactions catalyzed over nanotube catalysts patent, March 2004

Quantum Dot Bioconjugates for Ultrasensitive Nonisotopic Detection journal, September 1998

Engineered gene circuits journal, November 2002

Natural alleles at a tomato fruit size quantitative trait locus differ by heterochronic regulatory mutations journal, October 2002

Semiconductor Nanocrystals as Fluorescent Biological Labels patent, September 1998

Shaping carbon nanostructures by controlling the synthesis process journal, August 2001

Large-Scale Synthesis of Aligned Carbon Nanotubes journal, December 1996

Needle array and method of introducing biological substances into living cells using the needle array patent, October 1995

Synthetic DNA delivery systems journal, January 2000

Patterned growth of individual and multiple vertically aligned carbon nanofibers journal, June 2000

Animal cell line useful for expression of exogenous genes patent, October 1993

Preparation of Nucleic Acid Functionalized Carbon Nanotube Arrays journal, October 2002

A molecular delivery system by using AFM and nanoneedle journal, April 2005

Individually addressable vertically aligned carbon nanofiber-based electrochemical probes journal, March 2002

Field emission properties of different forms of carbon journal, June 2001

A synthetic oscillatory network of transcriptional regulators journal, January 2000

Carbon nanotube-enhanced electrochemical DNA biosensor for DNA hybridization detection journal, January 2003

Alignment mechanism of carbon nanofibers produced by plasma-enhanced chemical-vapor deposition journal, October 2001

Direct haplotyping of kilobase-size DNA using carbon nanotube probes
journal, July 2000

Fabrication and Characterization of Vertically Aligned Carbon Nanotubes on Silicon Substrates Using Porous Alumina Nanotemplates
journal, April 2002

Effects of spatial separation on the growth of vertically aligned carbon nanofibers produced by plasma-enhanced chemical vapor deposition
journal, January 2002

Carbon nanotube array-based biosensor
journal, January 2003

Major limitations in the use of cationic liposomes for DNA delivery
journal, March 1998

Nanoneedle chips and the production thereof
patent-application, April 2004

DNA-bridged carbon nanotube arrays
patent, October 2005

Microarrays of cells expressing defined cDNAs
journal, May 2001

Block and Graft Copolymers and Nanogel™ Copolymer Networks for DNA Delivery into Cell
journal, January 2000

The use of Chinese hamster ovary cells to quantify specific locus mutation and to determine mutagenicity of chemicals: A report of the GENE-TOX program
journal, March 1981

Micromachined interfaces: new approaches in cell immunoisolation and biomolecular separation
journal, October 2000

In Vivo Imaging of Quantum Dots Encapsulated in Phospholipid Micelles
journal, November 2002

Construction of a genetic toggle switch in Escherichia coli
journal, January 2000

Tracking Gene Expression after DNA Delivery Using Spatially Indexed Nanofiber Arrays
journal, July 2004

Field-emission studies of smooth and nanostructured carbon films
journal, August 1999

Rapid synthesis of carbon nanotubes and carbon encapsulated metal nanoparticles by a displacement reaction
patent, November 2002

Cell-manipulating apparatus and method of using the same
patent-application, December 2003

Gene expression using an ultrathin needle enabling accurate displacement and low invasiveness
journal, July 2005

Fabrication of Poly(ethylene glycol) Hydrogel Microstructures Using Photolithography
journal, September 2001

Covalently functionalized nanotubes as nanometre- sized probes in chemistry and biology
journal, July 1998

Nanoparticle delivery system
patent-application, April 2004

Nanotransfer and nanoreplication using deterministically grown sacrificial nanotemplates
patent-application, August 2005

Parallel macromolecular delivery and biochemical/electrochemical interface to cells employing nanostructures
patent-application, October 2004

Microfabricated Microneedles for Gene and Drug Delivery
journal, August 2000

Synthesis of Large Arrays of Well-Aligned Carbon Nanotubes on Glass
journal, November 1998

Microprojection array immunization patch and method
patent-application, December 2002

Nanotube Nanotweezers
journal, December 1999

Combinatorial Synthesis of Genetic Networks
journal, May 2002

Temporally resolved catecholamine spikes correspond to single vesicle release from individual chromaffin cells.
journal, December 1991

Remote electronic control of DNA hybridization through inductive coupling to an attached metal nanocrystal antenna
journal, January 2002

A galinstan expansion femtosyringe for microinjection of eukaryotic organelles and prokaryotes
journal, September 1999

Activation of the NADPH oxidase in human fibroblasts by mechanical intrusion of a single cell with an ultramicroelectrode
journal, March 1997

Gated fabrication of nanostructure field emission cathode material within a device
patent-application, November 2008

Structural and functional imaging with carbon nanotube AFM probes
journal, January 2001

Laterally Stabilized Complexes of DNA with Linear Reducible Polycations: Strategy for Triggered Intracellular Activation of DNA Delivery Vectors
journal, January 2002

Electrochemically induced adsorption of radio-labeled DNA on gold and HOPG substrates for STM investigations
journal, December 1991

The emerging field of nanotube biotechnology
journal, January 2003

DNA-functionalized single-walled carbon nanotubes
journal, September 2002

Modelling cellular behaviour
journal, January 2001

Carbon nanotube-containing catalysts, methods of making, and reactions catalyzed over nanotube catalysts
patent, March 2004

Quantum Dot Bioconjugates for Ultrasensitive Nonisotopic Detection
journal, September 1998

Engineered gene circuits
journal, November 2002

Natural alleles at a tomato fruit size quantitative trait locus differ by heterochronic regulatory mutations
journal, October 2002

Semiconductor Nanocrystals as Fluorescent Biological Labels
patent, September 1998

Shaping carbon nanostructures by controlling the synthesis process
journal, August 2001

Large-Scale Synthesis of Aligned Carbon Nanotubes
journal, December 1996

Needle array and method of introducing biological substances into living cells using the needle array
patent, October 1995

Synthetic DNA delivery systems
journal, January 2000

Patterned growth of individual and multiple vertically aligned carbon nanofibers
journal, June 2000

Animal cell line useful for expression of exogenous genes
patent, October 1993

Preparation of Nucleic Acid Functionalized Carbon Nanotube Arrays
journal, October 2002

A molecular delivery system by using AFM and nanoneedle
journal, April 2005

Individually addressable vertically aligned carbon nanofiber-based electrochemical probes
journal, March 2002

Field emission properties of different forms of carbon
journal, June 2001

A synthetic oscillatory network of transcriptional regulators
journal, January 2000

Carbon nanotube-enhanced electrochemical DNA biosensor for DNA hybridization detection
journal, January 2003

Alignment mechanism of carbon nanofibers produced by plasma-enhanced chemical-vapor deposition
journal, October 2001