Cyanine-based probe\tag-peptide pair fluorescence protein imaging and fluorescence protein imaging methods
Abstract
A molecular probe comprises two arsenic atoms and at least one cyanine based moiety. A method of producing a molecular probe includes providing a molecule having a first formula, treating the molecule with HgOAc, and subsequently transmetallizing with AsCl.sub.3. The As is liganded to ethanedithiol to produce a probe having a second formula. A method of labeling a peptide includes providing a peptide comprising a tag sequence and contacting the peptide with a biarsenical molecular probe. A complex is formed comprising the tag sequence and the molecular probe. A method of studying a peptide includes providing a mixture containing a peptide comprising a peptide tag sequence, adding a biarsenical probe to the mixture, and monitoring the fluorescence of the mixture.
- Inventors:
- Issue Date:
- Research Org.:
- Pacific Northwest National Laboratory (PNNL), Richland, WA (United States)
- Sponsoring Org.:
- USDOE
- OSTI Identifier:
- 1082850
- Patent Number(s):
- 8354499
- Application Number:
- 12/773,707
- Assignee:
- Battelle Memorial Institute (Richland, WA)
- Patent Classifications (CPCs):
-
C - CHEMISTRY C07 - ORGANIC CHEMISTRY C07K - PEPTIDES
C - CHEMISTRY C09 - DYES C09B - ORGANIC DYES OR CLOSELY-RELATED COMPOUNDS FOR PRODUCING DYES {, e.g. PIGMENTS},
- DOE Contract Number:
- AC0676RLO1830
- Resource Type:
- Patent
- Country of Publication:
- United States
- Language:
- English
- Subject:
- 60 APPLIED LIFE SCIENCES
Citation Formats
Mayer-Cumblidge, M. Uljana, and Cao, Haishi. Cyanine-based probe\tag-peptide pair fluorescence protein imaging and fluorescence protein imaging methods. United States: N. p., 2013.
Web.
Mayer-Cumblidge, M. Uljana, & Cao, Haishi. Cyanine-based probe\tag-peptide pair fluorescence protein imaging and fluorescence protein imaging methods. United States.
Mayer-Cumblidge, M. Uljana, and Cao, Haishi. Tue .
"Cyanine-based probe\tag-peptide pair fluorescence protein imaging and fluorescence protein imaging methods". United States. https://www.osti.gov/servlets/purl/1082850.
@article{osti_1082850,
title = {Cyanine-based probe\tag-peptide pair fluorescence protein imaging and fluorescence protein imaging methods},
author = {Mayer-Cumblidge, M. Uljana and Cao, Haishi},
abstractNote = {A molecular probe comprises two arsenic atoms and at least one cyanine based moiety. A method of producing a molecular probe includes providing a molecule having a first formula, treating the molecule with HgOAc, and subsequently transmetallizing with AsCl.sub.3. The As is liganded to ethanedithiol to produce a probe having a second formula. A method of labeling a peptide includes providing a peptide comprising a tag sequence and contacting the peptide with a biarsenical molecular probe. A complex is formed comprising the tag sequence and the molecular probe. A method of studying a peptide includes providing a mixture containing a peptide comprising a peptide tag sequence, adding a biarsenical probe to the mixture, and monitoring the fluorescence of the mixture.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {2013},
month = {1}
}
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Works referenced in this record:
Specific Covalent Labeling of Recombinant Protein Molecules Inside Live Cells
journal, July 1998
- Griffin, B. Albert; Adams, Stephen R.; Tsien, Roger Y.
- Science, Vol. 281, Issue 5374, p. 269-272
A Fusion of Disciplines: Chemical Approaches to Exploit Fusion Proteins for Functional Genomics
journal, September 2003
- Johnsson, Nils; Johnsson, Kai
- ChemBioChem, Vol. 4, Issue 9
Synthetic molecules that specifically react with target sequences
patent, February 2004
- Tsien, Roger Y.; Griffin, B. Albert
- US Patent Document 6,686,458
New Biarsenical Ligands and Tetracysteine Motifs for Protein Labeling in Vitro and in Vivo: Synthesis and Biological Applications
journal, May 2002
- Adams, Stephen R.; Campbell, Robert E.; Gross, Larry A.
- Journal of the American Chemical Society, Vol. 124, Issue 21
Reagents and procedures for high-specificity labeling
patent, November 2006
- Ebright, Richard H.; Ebright, Yon W.
- US Patent Document 7,141,655