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Title: Cyanine-based probe\tag-peptide pair fluorescence protein imaging and fluorescence protein imaging methods

Abstract

A molecular probe comprises two arsenic atoms and at least one cyanine based moiety. A method of producing a molecular probe includes providing a molecule having a first formula, treating the molecule with HgOAc, and subsequently transmetallizing with AsCl.sub.3. The As is liganded to ethanedithiol to produce a probe having a second formula. A method of labeling a peptide includes providing a peptide comprising a tag sequence and contacting the peptide with a biarsenical molecular probe. A complex is formed comprising the tag sequence and the molecular probe. A method of studying a peptide includes providing a mixture containing a peptide comprising a peptide tag sequence, adding a biarsenical probe to the mixture, and monitoring the fluorescence of the mixture.

Inventors:
;
Issue Date:
Research Org.:
Pacific Northwest National Lab. (PNNL), Richland, WA (United States)
Sponsoring Org.:
USDOE
OSTI Identifier:
1082850
Patent Number(s):
8354499
Application Number:
12/773,707
Assignee:
Battelle Memorial Institute (Richland, WA)
Patent Classifications (CPCs):
C - CHEMISTRY C09 - DYES C09B - ORGANIC DYES OR CLOSELY-RELATED COMPOUNDS FOR PRODUCING DYES {, e.g. PIGMENTS},
C - CHEMISTRY C07 - ORGANIC CHEMISTRY C07K - PEPTIDES
DOE Contract Number:  
AC0676RLO1830
Resource Type:
Patent
Country of Publication:
United States
Language:
English
Subject:
60 APPLIED LIFE SCIENCES

Citation Formats

Mayer-Cumblidge, M. Uljana, and Cao, Haishi. Cyanine-based probe\tag-peptide pair fluorescence protein imaging and fluorescence protein imaging methods. United States: N. p., 2013. Web.
Mayer-Cumblidge, M. Uljana, & Cao, Haishi. Cyanine-based probe\tag-peptide pair fluorescence protein imaging and fluorescence protein imaging methods. United States.
Mayer-Cumblidge, M. Uljana, and Cao, Haishi. Tue . "Cyanine-based probe\tag-peptide pair fluorescence protein imaging and fluorescence protein imaging methods". United States. https://www.osti.gov/servlets/purl/1082850.
@article{osti_1082850,
title = {Cyanine-based probe\tag-peptide pair fluorescence protein imaging and fluorescence protein imaging methods},
author = {Mayer-Cumblidge, M. Uljana and Cao, Haishi},
abstractNote = {A molecular probe comprises two arsenic atoms and at least one cyanine based moiety. A method of producing a molecular probe includes providing a molecule having a first formula, treating the molecule with HgOAc, and subsequently transmetallizing with AsCl.sub.3. The As is liganded to ethanedithiol to produce a probe having a second formula. A method of labeling a peptide includes providing a peptide comprising a tag sequence and contacting the peptide with a biarsenical molecular probe. A complex is formed comprising the tag sequence and the molecular probe. A method of studying a peptide includes providing a mixture containing a peptide comprising a peptide tag sequence, adding a biarsenical probe to the mixture, and monitoring the fluorescence of the mixture.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {2013},
month = {1}
}

Works referenced in this record:

Specific Covalent Labeling of Recombinant Protein Molecules Inside Live Cells
journal, July 1998


New Biarsenical Ligands and Tetracysteine Motifs for Protein Labeling in Vitro and in Vivo:  Synthesis and Biological Applications
journal, May 2002