Multiplex detection of agricultural pathogens
Abstract
Described are kits and methods useful for detection of agricultural pathogens in a sample. Genomic sequence information from agricultural pathogens was analyzed to identify signature sequences, e.g., polynucleotide sequences useful for confirming the presence or absence of a pathogen in a sample. Primer and probe sets were designed and optimized for use in a PCR based, multiplexed Luminex assay and/or an array assay to successfully identify the presence or absence of pathogens in a sample.
- Inventors:
- Issue Date:
- Research Org.:
- Lawrence Livermore National Laboratory (LLNL), Livermore, CA (United States)
- Sponsoring Org.:
- USDOE
- OSTI Identifier:
- 1082751
- Patent Number(s):
- 8354514
- Application Number:
- 12/842,952
- Assignee:
- Lawrence Livermore National Security, LLC (Livermore, CA)
- Patent Classifications (CPCs):
-
C - CHEMISTRY C12 - BIOCHEMISTRY C12Q - MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS
- DOE Contract Number:
- W-7405-ENG-48
- Resource Type:
- Patent
- Country of Publication:
- United States
- Language:
- English
- Subject:
- 60 APPLIED LIFE SCIENCES
Citation Formats
Siezak, Thomas R., Gardner, Shea, Torres, Clinton, Vitalis, Elizabeth, and Lenhoff, Raymond J. Multiplex detection of agricultural pathogens. United States: N. p., 2013.
Web.
Siezak, Thomas R., Gardner, Shea, Torres, Clinton, Vitalis, Elizabeth, & Lenhoff, Raymond J. Multiplex detection of agricultural pathogens. United States.
Siezak, Thomas R., Gardner, Shea, Torres, Clinton, Vitalis, Elizabeth, and Lenhoff, Raymond J. Tue .
"Multiplex detection of agricultural pathogens". United States. https://www.osti.gov/servlets/purl/1082751.
@article{osti_1082751,
title = {Multiplex detection of agricultural pathogens},
author = {Siezak, Thomas R. and Gardner, Shea and Torres, Clinton and Vitalis, Elizabeth and Lenhoff, Raymond J.},
abstractNote = {Described are kits and methods useful for detection of agricultural pathogens in a sample. Genomic sequence information from agricultural pathogens was analyzed to identify signature sequences, e.g., polynucleotide sequences useful for confirming the presence or absence of a pathogen in a sample. Primer and probe sets were designed and optimized for use in a PCR based, multiplexed Luminex assay and/or an array assay to successfully identify the presence or absence of pathogens in a sample.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {Tue Jan 15 00:00:00 EST 2013},
month = {Tue Jan 15 00:00:00 EST 2013}
}
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