Expanding the eukaryotic genetic code
Abstract
This invention provides compositions and methods for producing translational components that expand the number of genetically encoded amino acids in eukaryotic cells. The components include orthogonal tRNAs, orthogonal aminoacyl-tRNA synthetases, orthogonal pairs of tRNAs/synthetases and unnatural amino acids. Proteins and methods of producing proteins with unnatural amino acids in eukaryotic cells are also provided.
- Inventors:
- Issue Date:
- Research Org.:
- The Scripps Research Inst., La Jolla, CA (United States)
- Sponsoring Org.:
- USDOE
- OSTI Identifier:
- 1082728
- Patent Number(s):
- 8357532
- Application Number:
- 13/443,800
- Assignee:
- The Scripps Research Institute (La Jolla, CA)
- Patent Classifications (CPCs):
-
A - HUMAN NECESSITIES A61 - MEDICAL OR VETERINARY SCIENCE A61K - PREPARATIONS FOR MEDICAL, DENTAL, OR TOILET PURPOSES
A - HUMAN NECESSITIES A61 - MEDICAL OR VETERINARY SCIENCE A61P - SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- DOE Contract Number:
- FG03-00ER45812
- Resource Type:
- Patent
- Country of Publication:
- United States
- Language:
- English
- Subject:
- 60 APPLIED LIFE SCIENCES
Citation Formats
Chin, Jason W., Cropp, T. Ashton, Anderson, J. Christopher, and Schultz, Peter G. Expanding the eukaryotic genetic code. United States: N. p., 2013.
Web.
Chin, Jason W., Cropp, T. Ashton, Anderson, J. Christopher, & Schultz, Peter G. Expanding the eukaryotic genetic code. United States.
Chin, Jason W., Cropp, T. Ashton, Anderson, J. Christopher, and Schultz, Peter G. Tue .
"Expanding the eukaryotic genetic code". United States. https://www.osti.gov/servlets/purl/1082728.
@article{osti_1082728,
title = {Expanding the eukaryotic genetic code},
author = {Chin, Jason W. and Cropp, T. Ashton and Anderson, J. Christopher and Schultz, Peter G.},
abstractNote = {This invention provides compositions and methods for producing translational components that expand the number of genetically encoded amino acids in eukaryotic cells. The components include orthogonal tRNAs, orthogonal aminoacyl-tRNA synthetases, orthogonal pairs of tRNAs/synthetases and unnatural amino acids. Proteins and methods of producing proteins with unnatural amino acids in eukaryotic cells are also provided.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {Tue Jan 22 00:00:00 EST 2013},
month = {Tue Jan 22 00:00:00 EST 2013}
}
Works referenced in this record:
Glycoprotein synthesis
patent-application, July 2007
- Schultz, Peter; Wang, Lei; Zhang, Zhiwen
- US Patent Application 11/580203; 20070172915
Site specific incorporation of keto amino acids into proteins
patent-application, September 2008
- Schultz, Peter G.; Wang, Lei
- US Patent Application 11/978182; 20080227153
Engineering Chemical Reactivity on Cell Surfaces Through Oligosaccharide Biosynthesis
journal, May 1997
- Mahal, Lara K.; Yarema, Kevin J.; Bertozzi, Carolyn R.
- Science, Vol. 276, Issue 5315, p. 1125-1128
Unnatural reactive amino acid genetic code additions
patent-application, December 2004
- Deiters, Alexander; Cropp, T. Ashton; Chin, Jason W.
- US Patent Application 10/826919; 20040265952
Site specific incorporation of keto amino acids into proteins
patent-application, March 2006
- Schultz, Peter G.; Wang, Lei
- US Patent Application 10/530421; 20060068478
Glycoprotein synthesis
patent, February 2007
- Schultz, Peter G.; Wang, Lei; Zhang, Zhiwen
- US Patent Document 7,183,082
Selective incorporation of 5-hydroxytryptophan into proteins in mammalian cells
patent-application, May 2007
- Zhang, Zhiwen; Alfonta, Lital; Schultz, Peter
- US Patent Application 10/580987; 20070111193
Site-Specific Protein Modification Using a Ketone Handle
journal, January 1996
- Cornish, Virginia W.; Hahn, Klaus M.; Schultz, Peter G.
- Journal of the American Chemical Society, Vol. 118, Issue 34, p. 8150-8151
Incorporation of azides into recombinant proteins for chemoselective modification by the Staudinger ligation
journal, December 2001
- Kiick, K. L.; Saxon, E.; Tirrell, D. A.
- Proceedings of the National Academy of Sciences, Vol. 99, Issue 1, p. 19-24
Bioconjugation by Copper(I)-Catalyzed Azide-Alkyne [3 + 2] Cycloaddition
journal, March 2003
- Wang, Qian; Chan, Timothy R.; Hilgraf, Robert
- Journal of the American Chemical Society, Vol. 125, Issue 11, p. 3192-3193
Expanding the eukaryotic genetic code
patent-application, January 2005
- Chin, Jason W.; Cropp, T. Ashton; Anderson, J. Christopher
- US Patent Application 10/825867; 20050009049
An engineered Escherichia coli tyrosyl-tRNA synthetase for site-specific incorporation of an unnatural amino acid into proteins in eukaryotic translation and its application in a wheat germ cell-free system
journal, July 2002
- Kiga, D.; Sakamoto, K.; Kodama, K.
- Proceedings of the National Academy of Sciences, Vol. 99, Issue 15, p. 9715-9720
Compositions of orthogonal leucyl-trna and aminoacyl-trna synthetase pairs and uses thereof
patent-application, July 2006
- Anderson, J. Christopher; Schultz, Peter G.
- US Patent Application 10/563655; 20060160175
Addition of p-Azido-L -phenylalanine to the Genetic Code of Escherichia coli
journal, July 2002
- Chin, Jason W.; Santoro, Stephen W.; Martin, Andrew B.
- Journal of the American Chemical Society, Vol. 124, Issue 31, p. 9026-9027
An Expanded Eukaryotic Genetic Code
journal, August 2003
- Chin, Jason W.; Cropp, T. Ashton; Anderson, J. Christopher
- Science, Vol. 301, Issue 5635, p. 964-967
A New Strategy for the Site-Specific Modification of Proteins in Vivo
journal, June 2003
- Zhang, Zhiwen; Smith, Brian A. C.; Wang, Lei
- Biochemistry, Vol. 42, Issue 22, p. 6735-6746
Site-specific labeling of proteins for NMR studies
patent-application, May 2006
- Deiters, Alexander; Geierstanger, Bernhard H.; Schultz, Peter G.
- US Patent Application 11/233466; 20060110784
Site-specific incorporation of biotinylated amino acids to identify surface-exposed residues in integral membrane proteins
journal, October 1997
- Gallivan, Justin P.; Lester, Henry A.; Dougherty, Dennis A.
- Chemistry & Biology, Vol. 4, Issue 10, p. 739-749
Site-specific incorporation of redox active amino acids into proteins
patent-application, October 2005
- Alfonta, Lital; Schultz, Peter G.; Zhang, Zhiwen
- US Patent Application 10/965218; 20050227318
Selective posttranslational modification of phage-displayed polypeptides
patent-application, August 2007
- Tsao, Meng-Lin; Tian, Feng; Schultz, Peter
- US Patent Application 11/580223; 20070178448
Misacylation of Yeast Amber Suppressor tRNATyr by E. coli Lysyl-tRNA Synthetase and Its Effective Repression by Genetic Engineering of the tRNA Sequence
journal, April 2006
- Fukunaga, Jun-ichi; Yokogawa, Takashi; Ohno, Satoshi
- The Journal of Biochemistry, Vol. 139, Issue 4
Expanding the eukaryotic genetic code
patent, November 2009
- Chin, Jason W.; Cropp, T. Ashton; Anderson, J. Christopher
- US Patent Document 7,618,775
Glycoprotein synthesis
patent, April 2007
- Shultz, Peter G.; Wang, Lei; Zhang, Zhiwen
- US Patent Document 7,199,222
Site specific incorporation of heavy atom-containing unnatural amino acids into proteins for structure determination
patent-application, December 2005
- Xie, Jianming; Wang, Lei; Wu, Ning
- US Patent Application 11/137850; 20050272121
Chemoselective ligation reactions with proteins, oligosaccharides and cells
journal, December 1998
- Lemieux, George A.; Bertozzi, Carolyn R.
- Trends in Biotechnology, Vol. 16, Issue 12, p. 506-513
Glycoprotein synthesis
patent, November 2010
- Schultz, Peter G.; Wang, Lei; Zhang, Zhiwen
- US Patent Document 7,834,159
Site-specific incorporation of redox active amino acids into proteins
patent-application, January 2007
- Alfonta, Lital; Schultz, Peter G.; Zhang, Zhiwen
- US Patent Application 10/575991; 20070009990
Expanding the eukaryotic genetic code
patent-application, July 2007
- Chin, Jason; Cropp, T. Ashton; Anderson, J. Christopher
- US Patent Application 11/710672; 20070166791
Intermolecular 1,3-Dipolar Cycloadditions
book, January 1991
- Padwa, Albert
- Comprehensive Organic Synthesis
Compositions of orthogonal lysyl-trna and aminoacyl-trna synthetase pairs and uses thereof
patent-application, February 2007
- Anderson, J. Christopher; Wu, Ning; Santoro, Stephen
- US Patent Application 10/563686; 20070042461
Aliphatic amino azides as key building blocks for efficient polyamine syntheses
journal, July 1993
- Carboni, Bertrand; Benalil, Aziza; Vaultier, Michel
- The Journal of Organic Chemistry, Vol. 58, Issue 14
Partition of tRNA synthetases into two classes based on mutually exclusive sets of sequence motifs
journal, September 1990
- Eriani, Gilbert; Delarue, Marc; Poch, Olivier
- Nature, Vol. 347, Issue 6289, p. 203-206
Adding l-3-(2-Naphthyl)alanine to the Genetic Code of E. coli
journal, March 2002
- Wang, Lei; Brock, Ansgar; Schultz, Peter G.
- Journal of the American Chemical Society, Vol. 124, Issue 9, p. 1836-1837
Recent advances in technology for measuring and manipulating cell signals
journal, June 2000
- Zacharias, David A.; Baird, Geoffrey S.; Tsien, Roger Y.
- Current Opinion in Neurobiology, Vol. 10, Issue 3, p. 416-421
Methods and composition for the production of orthogonal tRNA-aminoacyltRNA synthetase pairs
patent-application, October 2006
- Schultz, Peter; Wang, Lei; Anderson, John Christopher
- US Patent Application 11/254170; 20060234367
In vivo incorporation of alkynyl amino acids into proteins in eubacteria
patent-application, April 2006
- Deiters, Alexander; Schultz, Peter
- US Patent Application 11/232425; 20060073507
patent-application, October 2004
- Schultz, Peter G.; Wang, Lei
- US Patent Application 10/744899; 20040198637
Expanding tRNA recognition of a tRNA synthetase by a single amino acid change
journal, May 2003
- Feng, L.; Tumbula-Hansen, D.; Toogood, H.
- Proceedings of the National Academy of Sciences, Vol. 100, Issue 10, p. 5676-5681
Addition of the keto functional group to the genetic code of Escherichia coli
journal, December 2002
- Wang, L.; Zhang, Z.; Brock, A.
- Proceedings of the National Academy of Sciences, Vol. 100, Issue 1, p. 56-61
Unnatural amino acids as probes of protein structure and function
journal, December 2000
- Dougherty, D.
- Current Opinion in Chemical Biology, Vol. 4, Issue 6, p. 645-652
Methods and composition for the production of orthogonal tRNA-aminoacyltRNA synthetase pairs
patent-application, March 2006
- Schultz, Peter; Wang, Lei; Anderson, John Christopher
- US Patent Application 11/002387; 20060063244
Progress toward the evolution of an organism with an expanded genetic code
journal, April 1999
- Liu, David R.; Schultz, Peter G.
- Proceedings of the National Academy of Sciences, Vol. 96, Issue 9, p. 4780-4785
Decomposition of Protein Tryptophan Fluorescence Spectra into Log-Normal Components. II. The Statistical Proof of Discreteness of Tryptophan Classes in Proteins
journal, September 2001
- Reshetnyak, Yana K.; Burstein, Edward A.
- Biophysical Journal, Vol. 81, Issue 3, p. 1710-1734
Specific Covalent Labeling of Recombinant Protein Molecules Inside Live Cells
journal, July 1998
- Griffin, B. Albert; Adams, Stephen R.; Tsien, Roger Y.
- Science, Vol. 281, Issue 5374, p. 269-272
Exploring the Limits of Codon and Anticodon Size
journal, February 2002
- Anderson, J. Christopher; Magliery, Thomas J.; Schultz, Peter G.
- Chemistry & Biology, Vol. 9, Issue 2, p. 237-244
Structurally Modified Firefly Luciferase. Effects of Amino Acid Substitution at Position 286
journal, November 1997
- Arslan, Tuncer; Mamaev, Sergey V.; Mamaeva, Natalia V.
- Journal of the American Chemical Society, Vol. 119, Issue 45, p. 10877-10887
Glycoprotein synthesis
patent, August 2005
- Schultz, Peter G.; Wang, Lei; Zhang, Zhiwen
- US Patent Document 6,927,042
Regulated phosphorylation and dephosphorylation of GAL4, a transcriptional activator.
journal, August 1989
- Mylin, L. M.; Bhat, J. P.; Hopper, J. E.
- Genes & Development, Vol. 3, Issue 8, p. 1157-1165
Protein Tyrosine Kinase Csk-Catalyzed Phosphorylation of Src Containing Unnatural Tyrosine Analogues
journal, September 2001
- Wang, Dongxia; Cole, Philip A.
- Journal of the American Chemical Society, Vol. 123, Issue 37, p. 8883-8886
Compositions of orthogonal lysyl-tRNA and aminoacyl-tRNA synthetase pairs and uses thereof
patent-application, August 2006
- Anderson, J. Christopher; Wu, Ning; Santoro, Stephen
- US Patent Application 11/326970; 20060177900
Expanding the eukaryotic genetic code
patent-application, July 2007
- Chin, Jason W.; Cropp, T. Ashton; Anderson, J. Christopher
- US Patent Application 11/710655; 20070154952
Methods and composition for the production of orthogonal tRNA-aminoacyltRNA synthetase pairs
patent-application, September 2005
- Schultz, Peter; Wang, Lei; Anderson, John Christopher
- US Patent Application 11/009635; 20050208536
Glycoprotein synthesis
patent, May 2007
- Schultz, Peter G.; Wang, Lei; Zhang, Zhiwen
- US Patent Document 7,217,809
Methods and compositions for the production of orthogonal tRNA-aminoacyl tRNA synthetase pairs
patent, August 2006
- Schultz, Peter G.; Wang, Lei; Anderson, John Christopher
- US Patent Document 7,083,970
Activity-Based Protein Profiling in Vivo Using a Copper(I)-Catalyzed Azide-Alkyne [3 + 2] Cycloaddition
journal, April 2003
- Speers, Anna E.; Adam, Gregory C.; Cravatt, Benjamin F.
- Journal of the American Chemical Society, Vol. 125, Issue 16, p. 4686-4687
Peptidotriazoles on Solid Phase [1,2,3]-Triazoles by Regiospecific Copper(I)-Catalyzed 1,3-Dipolar Cycloadditions of Terminal Alkynes to Azides
journal, May 2002
- Tornøe, Christian W.; Christensen, Caspar; Meldal, Morten
- The Journal of Organic Chemistry, Vol. 67, Issue 9, p. 3057-3064
Addition of a photocrosslinking amino acid to the genetic code of Escherichia coli
journal, August 2002
- Chin, J. W.; Martin, A. B.; King, D. S.
- Proceedings of the National Academy of Sciences, Vol. 99, Issue 17, p. 11020-11024
Glycoprotein synthesis
patent-application, August 2007
- Schultz, Peter; Wang, Lei; Zhang, Zhiwen
- US Patent Application 11/520849; 20070184517
Expanding the genetic code: selection of efficient suppressors of four-base codons and identification of “shifty” four-base codons with a library approach in Escherichia coli
journal, March 2001
- Magliery, Thomas J.; Anderson, J. Christopher; Schultz, Peter G.
- Journal of Molecular Biology, Vol. 307, Issue 3, p. 755-769
Glycoprotein synthesis
patent, July 2007
- Schultz, Peter G.; Wang, Lei; Zhang, Zhiwen
- US Patent Document 7,238,510
Aminoacyl-tRNA synthetases: Versatile players in the changing theater of translation
journal, November 2002
- Francklyn, Christopher; Perona, John J.; Puetz, Joern
- RNA, Vol. 8, Issue 11
Imaging biochemistry inside cells
journal, May 2001
- Wouters, Fred S.; Verveer, Peter J.; Bastiaens, Philippe I.H.
- Trends in Cell Biology, Vol. 11, Issue 5, p. 203-211
Adding photoregulated amino acids to the genetic code
patent-application, June 2006
- Deiters, Alexander; Wu, Ning; Schultz, Peter G.
- US Patent Application 11/233508; 20060134746
Glycoprotein synthesis
patent, October 2006
- Schultz, Peter G.; Wang, Lei; Zhang, Zhiwen
- US Patent Document 7,129,333
Composition of orthogonal glutamyl-trna and aminoacyl-trna synthetase pairs and uses thereof
patent-application, January 2007
- Anderson, J. Christopher; Schultz, Peter G.; Santoro, Stephen
- US Patent Application 10/563656; 20070020634
Preparation of biological labels with acetylenic linker arms
journal, January 1997
- Crisp, Geoffrey T.; Gore, Jeffrey
- Tetrahedron, Vol. 53, Issue 4, p. 1505-1522
Glycoprotein synthesis
patent, August 2007
- Schultz, Peter G.; Wang, Lei; Zhang, Zhiwen
- US Patent Document 7,262,040
A bacterial amber suppressor in Saccharomyces cerevisiae is selectively recognized by a bacterial aminoacyl-tRNA synthetase
journal, April 1990
- Edwards, H.; Schimmel, P.
- Molecular and Cellular Biology, Vol. 10, Issue 4
A Mutant Escherichia coli Tyrosyl-tRNA Synthetase Utilizes the Unnatural Amino Acid Azatyrosine More Efficiently than Tyrosine
journal, September 2000
- Hamano-Takaku, Fumie; Iwama, Toshiharu; Saito-Yano, Shio
- Journal of Biological Chemistry, Vol. 275, Issue 51, p. 40324-40328
Selective incorporation of 5-hydroxytryptophan into proteins in mammalian cells
patent-application, June 2005
- Zhang, Zhiwen; Alfonta, Lital; Schultz, Peter
- US Patent Application 11/016348; 20050136513
Adding Amino Acids with Novel Reactivity to the Genetic Code of Saccharomyces Cerevisiae
journal, October 2003
- Deiters, Alexander; Cropp, T. Ashton; Mukherji, Mridul
- Journal of the American Chemical Society, Vol. 125, Issue 39, p. 11782-11783
Expanding the genetic code
journal, December 2001
- Wang, Lei; Schultz, Peter G.
- Chemical Communications, Issue 1
In vivo incorporation of unnatural amino acids
patent, May 2006
- Schultz, Peter G.; Wang, Lei; Anderson, John Christopher
- US Patent Document 7,045,337
Genetically programmed expression of proteins containing the unnatural amino acid phenylselenocysteine
patent-application, October 2007
- Wang, Jiangyun; Schultz, Peter
- US Patent Application 11/724039; 20070238152
An expanding genetic code
journal, December 2004
- Cropp, T. Ashton; Schultz, Peter G.
- Trends in Genetics, Vol. 20, Issue 12, p. 625-630
Progress Toward an Expanded Eukaryotic Genetic Code
journal, June 2003
- Chin, Jason W.; Cropp, T. Ashton; Chu, Stephanie
- Chemistry & Biology, Vol. 10, Issue 6, p. 511-519
Cellular screening assays using fluorescence microscopy
journal, October 2001
- Blake, R.
- Current Opinion in Pharmacology, Vol. 1, Issue 5, p. 533-539
Unnatural reactive amino acid genetic code additions
patent-application, November 2006
- Deiters, Alexander; Cropp, T. Ashton; Chin, Jason W.
- US Patent Application 10/561121; 20060246509
Programming peptidomimetic syntheses by translating genetic codes designed de novo
journal, May 2003
- Forster, A. C.; Tan, Z.; Nalam, M. N. L.
- Proceedings of the National Academy of Sciences, Vol. 100, Issue 11, p. 6353-6357
Import of amber and ochre suppressor tRNAs into mammalian cells: A general approach to site-specific insertion of amino acid analogues into proteins
journal, November 2001
- Kohrer, C.; Xie, L.; Kellerer, S.
- Proceedings of the National Academy of Sciences, Vol. 98, Issue 25, p. 14310-14315
In vivo site-specific incorporation of N-acetyl-galactosamine amino acids in eubacteria
patent-application, May 2006
- Schultz, Peter G.; Hanson, Sarah R.; Xu, Ran
- US Patent Application 11/255601; 20060110796
Expanding the Genetic Code of Escherichia coli
journal, April 2001
- Wang, L.; Brock, A.; Herberich, B.
- Science, Vol. 292, Issue 5516, p. 498-500
Synthesis and Photophysical Properties of Fluorescent Derivatives of Methylmercury
journal, January 1996
- Bolletta, Fabrizio; Fabbri, Daniele; Lombardo, Marco
- Organometallics, Vol. 15, Issue 9, p. 2415-2417
Site-specific incorporation of an unnatural amino acid into proteins in mammalian cells
journal, November 2002
- Sakamoto, K.
- Nucleic Acids Research, Vol. 30, Issue 21, p. 4692-4699