Promoters and proteins from Clostridium thermocellum and uses thereof
Abstract
The present invention relates to an inducible and a high expression nucleic acid promoter isolated from Clostridium thermocellum. These promoters are useful for directing expression of a protein or polypeptide encoded by a nucleic acid molecule operably associated with the nucleic acid promoters. The present invention also relates to nucleic acid constructs including the C. thermocellum promoters, and expression vectors and hosts containing such nucleic acid constructs. The present invention also relates to protein isolated from Clostridium thermocellum, including a repressor protein. The present invention also provides methods of using the isolated promoters and proteins from Clostridium thermocellum, including methods for directing inducible in vitro and in vivo expression of a protein or polypeptide in a host, and methods of producing ethanol from a cellulosic biomass.
- Inventors:
- Issue Date:
- Research Org.:
- Univ. of Rochester, NY (United States)
- Sponsoring Org.:
- USDOE
- OSTI Identifier:
- 1080416
- Patent Number(s):
- 8309324
- Application Number:
- 11/271,287
- Assignee:
- University of Rochester (Rochester, NY)
- Patent Classifications (CPCs):
-
C - CHEMISTRY C07 - ORGANIC CHEMISTRY C07K - PEPTIDES
C - CHEMISTRY C12 - BIOCHEMISTRY C12P - FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE {
- DOE Contract Number:
- FG02-94ER20155
- Resource Type:
- Patent
- Country of Publication:
- United States
- Language:
- English
- Subject:
- 59 BASIC BIOLOGICAL SCIENCES
Citation Formats
Wu, J. H. David, and Newcomb, Michael. Promoters and proteins from Clostridium thermocellum and uses thereof. United States: N. p., 2012.
Web.
Wu, J. H. David, & Newcomb, Michael. Promoters and proteins from Clostridium thermocellum and uses thereof. United States.
Wu, J. H. David, and Newcomb, Michael. Tue .
"Promoters and proteins from Clostridium thermocellum and uses thereof". United States. https://www.osti.gov/servlets/purl/1080416.
@article{osti_1080416,
title = {Promoters and proteins from Clostridium thermocellum and uses thereof},
author = {Wu, J. H. David and Newcomb, Michael},
abstractNote = {The present invention relates to an inducible and a high expression nucleic acid promoter isolated from Clostridium thermocellum. These promoters are useful for directing expression of a protein or polypeptide encoded by a nucleic acid molecule operably associated with the nucleic acid promoters. The present invention also relates to nucleic acid constructs including the C. thermocellum promoters, and expression vectors and hosts containing such nucleic acid constructs. The present invention also relates to protein isolated from Clostridium thermocellum, including a repressor protein. The present invention also provides methods of using the isolated promoters and proteins from Clostridium thermocellum, including methods for directing inducible in vitro and in vivo expression of a protein or polypeptide in a host, and methods of producing ethanol from a cellulosic biomass.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {Tue Nov 13 00:00:00 EST 2012},
month = {Tue Nov 13 00:00:00 EST 2012}
}
Works referenced in this record:
Lic16A of Clostridium thermocellum, a non-cellulosomal, highly complex endo- -1,3-glucanase bound to the outer cell surface
journal, April 2003
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Transcription of Clostridium thermocellum endoglucanase genes celF and celD.
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- Journal of Bacteriology, Vol. 173, Issue 1
Nucleotide Sequence of celC307 Encoding Endoglucanase C307 of Clostridium sp. Strain F1
journal, February 1991
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Cellulase, Clostridia, and Ethanol
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