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Title: Promoters and proteins from Clostridium thermocellum and uses thereof

Abstract

The present invention relates to an inducible and a high expression nucleic acid promoter isolated from Clostridium thermocellum. These promoters are useful for directing expression of a protein or polypeptide encoded by a nucleic acid molecule operably associated with the nucleic acid promoters. The present invention also relates to nucleic acid constructs including the C. thermocellum promoters, and expression vectors and hosts containing such nucleic acid constructs. The present invention also relates to protein isolated from Clostridium thermocellum, including a repressor protein. The present invention also provides methods of using the isolated promoters and proteins from Clostridium thermocellum, including methods for directing inducible in vitro and in vivo expression of a protein or polypeptide in a host, and methods of producing ethanol from a cellulosic biomass.

Inventors:
;
Issue Date:
Research Org.:
Univ. of Rochester, NY (United States)
Sponsoring Org.:
USDOE
OSTI Identifier:
1080416
Patent Number(s):
8309324
Application Number:
11/271,287
Assignee:
University of Rochester (Rochester, NY)
Patent Classifications (CPCs):
Y - NEW / CROSS SECTIONAL TECHNOLOGIES Y02 - TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE Y02E - REDUCTION OF GREENHOUSE GAS [GHG] EMISSIONS, RELATED TO ENERGY GENERATION, TRANSMISSION OR DISTRIBUTION
C - CHEMISTRY C07 - ORGANIC CHEMISTRY C07K - PEPTIDES
DOE Contract Number:  
FG02-94ER20155
Resource Type:
Patent
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES

Citation Formats

Wu, J. H. David, and Newcomb, Michael. Promoters and proteins from Clostridium thermocellum and uses thereof. United States: N. p., 2012. Web.
Wu, J. H. David, & Newcomb, Michael. Promoters and proteins from Clostridium thermocellum and uses thereof. United States.
Wu, J. H. David, and Newcomb, Michael. Tue . "Promoters and proteins from Clostridium thermocellum and uses thereof". United States. https://www.osti.gov/servlets/purl/1080416.
@article{osti_1080416,
title = {Promoters and proteins from Clostridium thermocellum and uses thereof},
author = {Wu, J. H. David and Newcomb, Michael},
abstractNote = {The present invention relates to an inducible and a high expression nucleic acid promoter isolated from Clostridium thermocellum. These promoters are useful for directing expression of a protein or polypeptide encoded by a nucleic acid molecule operably associated with the nucleic acid promoters. The present invention also relates to nucleic acid constructs including the C. thermocellum promoters, and expression vectors and hosts containing such nucleic acid constructs. The present invention also relates to protein isolated from Clostridium thermocellum, including a repressor protein. The present invention also provides methods of using the isolated promoters and proteins from Clostridium thermocellum, including methods for directing inducible in vitro and in vivo expression of a protein or polypeptide in a host, and methods of producing ethanol from a cellulosic biomass.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {2012},
month = {11}
}

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Works referenced in this record:

Transcription of Clostridium thermocellum endoglucanase genes celF and celD.
journal, January 1991


Nucleotide Sequence of celC307 Encoding Endoglucanase C307 of Clostridium sp. Strain F1
journal, February 1991


Cellulase, Clostridia, and Ethanol
journal, March 2005