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Title: Superactive cellulase formulation using cellobiohydrolase-1 from Penicillium funiculosum

Abstract

Purified cellobiohydrolase I (glycosyl hydrolase family 7 (Cel7A)) enzymes from Penicillium funiculosum demonstrate a high level of specific performance in comparison to other Cel7 family member enzymes when formulated with purified EIcd endoglucanase from A. cellulolyticus and tested on pretreated corn stover. This result is true of the purified native enzyme, as well as recombinantly expressed enzyme, for example, that enzyme expressed in a non-native Aspergillus host. In a specific example, the specific performance of the formulation using purified recombinant Cel7A from Penicillium funiculosum expressed in A. awamori is increased by more than 200% when compared to a formulation using purified Cel7A from Trichoderma reesei.

Inventors:
; ; ; ; ;
Issue Date:
Research Org.:
National Renewable Energy Lab. (NREL), Golden, CO (United States)
Sponsoring Org.:
USDOE
OSTI Identifier:
1079492
Patent Number(s):
8283150
Application Number:
12/247,594
Assignee:
Alliance for Sustainable Energy, LLC (Golden, CO)
Patent Classifications (CPCs):
C - CHEMISTRY C12 - BIOCHEMISTRY C12N - MICROORGANISMS OR ENZYMES
C - CHEMISTRY C12 - BIOCHEMISTRY C12Y - ENZYMES
DOE Contract Number:  
AC36-08GO28308
Resource Type:
Patent
Country of Publication:
United States
Language:
English
Subject:
60 APPLIED LIFE SCIENCES

Citation Formats

Adney, William S., Baker, John O., Decker, Stephen R., Chou, Yat-Chen, Himmel, Michael E., and Ding, Shi-You. Superactive cellulase formulation using cellobiohydrolase-1 from Penicillium funiculosum. United States: N. p., 2012. Web.
Adney, William S., Baker, John O., Decker, Stephen R., Chou, Yat-Chen, Himmel, Michael E., & Ding, Shi-You. Superactive cellulase formulation using cellobiohydrolase-1 from Penicillium funiculosum. United States.
Adney, William S., Baker, John O., Decker, Stephen R., Chou, Yat-Chen, Himmel, Michael E., and Ding, Shi-You. Tue . "Superactive cellulase formulation using cellobiohydrolase-1 from Penicillium funiculosum". United States. https://www.osti.gov/servlets/purl/1079492.
@article{osti_1079492,
title = {Superactive cellulase formulation using cellobiohydrolase-1 from Penicillium funiculosum},
author = {Adney, William S. and Baker, John O. and Decker, Stephen R. and Chou, Yat-Chen and Himmel, Michael E. and Ding, Shi-You},
abstractNote = {Purified cellobiohydrolase I (glycosyl hydrolase family 7 (Cel7A)) enzymes from Penicillium funiculosum demonstrate a high level of specific performance in comparison to other Cel7 family member enzymes when formulated with purified EIcd endoglucanase from A. cellulolyticus and tested on pretreated corn stover. This result is true of the purified native enzyme, as well as recombinantly expressed enzyme, for example, that enzyme expressed in a non-native Aspergillus host. In a specific example, the specific performance of the formulation using purified recombinant Cel7A from Penicillium funiculosum expressed in A. awamori is increased by more than 200% when compared to a formulation using purified Cel7A from Trichoderma reesei.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {2012},
month = {10}
}

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Works referenced in this record:

Evaluation of the cellobiose-fermenting yeastBrettanomyces custersii in the simultaneous saccharification and fermentation of cellulose
journal, May 1992


Family 7 cellobiohydrolases from Phanerochaete chrysosporium: crystal structure of the catalytic module of Cel7D (CBH58) at 1.32 Å resolution and homology models of the isozymes
journal, December 2001


Characterization of the major endo-1,4-β-d-glucanases from the cellulase of Penicillium pinophilum/funiculosum
journal, February 1989


Hydrolysis of cellulose using ternary mixtures of purified celluloses
journal, March 1998


The isolation, purification and properties of the cellobiohydrolase component of Penicillium funiculosum cellulase
journal, July 1980


Simultaneous saccharification and fermentation (SSF) of lignocellulosics to ethanol under vacuum cycling and step feeding
journal, April 1984