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Title: Detection of nucleic acids by multiple sequential invasive cleavages

Abstract

The present invention relates to means for the detection and characterization of nucleic acid sequences, as well as variations in nucleic acid sequences. The present invention also relates to methods for forming a nucleic acid cleavage structure on a target sequence and cleaving the nucleic acid cleavage structure in a site-specific manner. The structure-specific nuclease activity of a variety of enzymes is used to cleave the target-dependent cleavage structure, thereby indicating the presence of specific nucleic acid sequences or specific variations thereof. The present invention further relates to methods and devices for the separation of nucleic acid molecules based on charge. The present invention also provides methods for the detection of non-target cleavage products via the formation of a complete and activated protein binding region. The invention further provides sensitive and specific methods for the detection of human cytomegalovirus nucleic acid in a sample.

Inventors:
; ; ;
Issue Date:
Research Org.:
Third Wave Technologies, Inc. (Madison, WI)
Sponsoring Org.:
USDOE
OSTI Identifier:
1079451
Patent Number(s):
8288093
Application Number:
12/099,656
Assignee:
Third Wave Technologies, Inc. (Madison, WI)
Patent Classifications (CPCs):
C - CHEMISTRY C12 - BIOCHEMISTRY C12Q - MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS
Y - NEW / CROSS SECTIONAL TECHNOLOGIES Y10 - TECHNICAL SUBJECTS COVERED BY FORMER USPC Y10S - TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
DOE Contract Number:  
FG02-94ER81891
Resource Type:
Patent
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES

Citation Formats

Hall, Jeff G, Lyamichev, Victor I, Mast, Andrea L, and Brow, Mary Ann D. Detection of nucleic acids by multiple sequential invasive cleavages. United States: N. p., 2012. Web.
Hall, Jeff G, Lyamichev, Victor I, Mast, Andrea L, & Brow, Mary Ann D. Detection of nucleic acids by multiple sequential invasive cleavages. United States.
Hall, Jeff G, Lyamichev, Victor I, Mast, Andrea L, and Brow, Mary Ann D. Tue . "Detection of nucleic acids by multiple sequential invasive cleavages". United States. https://www.osti.gov/servlets/purl/1079451.
@article{osti_1079451,
title = {Detection of nucleic acids by multiple sequential invasive cleavages},
author = {Hall, Jeff G and Lyamichev, Victor I and Mast, Andrea L and Brow, Mary Ann D},
abstractNote = {The present invention relates to means for the detection and characterization of nucleic acid sequences, as well as variations in nucleic acid sequences. The present invention also relates to methods for forming a nucleic acid cleavage structure on a target sequence and cleaving the nucleic acid cleavage structure in a site-specific manner. The structure-specific nuclease activity of a variety of enzymes is used to cleave the target-dependent cleavage structure, thereby indicating the presence of specific nucleic acid sequences or specific variations thereof. The present invention further relates to methods and devices for the separation of nucleic acid molecules based on charge. The present invention also provides methods for the detection of non-target cleavage products via the formation of a complete and activated protein binding region. The invention further provides sensitive and specific methods for the detection of human cytomegalovirus nucleic acid in a sample.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {2012},
month = {10}
}

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