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Title: Interfacing nanostructures to biological cells

Abstract

Disclosed herein are methods and materials by which nanostructures such as carbon nanotubes, nanorods, etc. are bound to lectins and/or polysaccharides and prepared for administration to cells. Also disclosed are complexes comprising glycosylated nanostructures, which bind selectively to cells expressing glycosylated surface molecules recognized by the lectin. Exemplified is a complex comprising a carbon nanotube functionalized with a lipid-like alkane, linked to a polymer bearing repeated .alpha.-N-acetylgalactosamine sugar groups. This complex is shown to selectively adhere to the surface of living cells, without toxicity. In the exemplified embodiment, adherence is mediated by a multivalent lectin, which binds both to the cells and the .alpha.-N-acetylgalactosamine groups on the nanostructure.

Inventors:
; ;
Issue Date:
Research Org.:
The Regents of the University of California (Oakland, CA); Lawrence Berkeley National Lab. (LBNL), Berkeley, CA (United States)
Sponsoring Org.:
USDOE
OSTI Identifier:
1078298
Patent Number(s):
8257932
Application Number:
12/034,388
Assignee:
The Regents of the University of California (Oakland, CA)
Patent Classifications (CPCs):
A - HUMAN NECESSITIES A61 - MEDICAL OR VETERINARY SCIENCE A61K - PREPARATIONS FOR MEDICAL, DENTAL, OR TOILET PURPOSES
A - HUMAN NECESSITIES A61 - MEDICAL OR VETERINARY SCIENCE A61P - SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
DOE Contract Number:  
AC02-05CH11231
Resource Type:
Patent
Country of Publication:
United States
Language:
English
Subject:
77 NANOSCIENCE AND NANOTECHNOLOGY; 59 BASIC BIOLOGICAL SCIENCES

Citation Formats

Chen, Xing, Bertozzi, Carolyn R., and Zettl, Alexander K. Interfacing nanostructures to biological cells. United States: N. p., 2012. Web.
Chen, Xing, Bertozzi, Carolyn R., & Zettl, Alexander K. Interfacing nanostructures to biological cells. United States.
Chen, Xing, Bertozzi, Carolyn R., and Zettl, Alexander K. Tue . "Interfacing nanostructures to biological cells". United States. https://www.osti.gov/servlets/purl/1078298.
@article{osti_1078298,
title = {Interfacing nanostructures to biological cells},
author = {Chen, Xing and Bertozzi, Carolyn R. and Zettl, Alexander K.},
abstractNote = {Disclosed herein are methods and materials by which nanostructures such as carbon nanotubes, nanorods, etc. are bound to lectins and/or polysaccharides and prepared for administration to cells. Also disclosed are complexes comprising glycosylated nanostructures, which bind selectively to cells expressing glycosylated surface molecules recognized by the lectin. Exemplified is a complex comprising a carbon nanotube functionalized with a lipid-like alkane, linked to a polymer bearing repeated .alpha.-N-acetylgalactosamine sugar groups. This complex is shown to selectively adhere to the surface of living cells, without toxicity. In the exemplified embodiment, adherence is mediated by a multivalent lectin, which binds both to the cells and the .alpha.-N-acetylgalactosamine groups on the nanostructure.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {2012},
month = {9}
}

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Works referenced in this record:

Length control and sharpening of atomic force microscope carbon nanotube tips assisted by an electron beam
journal, September 2005


Multi-walled carbon nanotube interactions with human epidermal keratinocytes
journal, March 2005


Receptor Mediated Targeting of Lectin Conjugated Gliadin Nanoparticles in the Treatment of Helicobacter pylori
journal, August 2003


Inhibition of Escherichia coli heat-labile enterotoxin by neoglycoprotein and anti-lectin antibodies which mimic GM1 receptor
journal, October 2002


Biomimetic Engineering of Carbon Nanotubes by Using Cell Surface Mucin Mimics
journal, November 2004


Lectin-mediated transport of nanoparticles across Caco-2 and OK cells
journal, November 1999


Synthetic concanavalin A receptors and erythrocyte agglutination
journal, April 1978


Interfacing Carbon Nanotubes with Living Cells
journal, May 2006


Printed covalent glycan array for ligand profiling of diverse glycan binding proteins
journal, November 2004


Airway goblet cell mucin: its structure and regulation of secretion
journal, November 1997


In vivo biodistribution and highly efficient tumour targeting of carbon nanotubes in mice
journal, December 2006


Identification and Characterization of a Novel Human Collectin CL-K1
journal, December 2006


Structural diversity and specific distribution of O-glycans in normal human mucins along the intestinal tract
journal, November 2004


Structure and biosynthesis of human salivary mucins.
journal, December 2000


Tumor-specific targeting of a cell line with natural killer cell activity by asialoglycoprotein receptor gene transfer
journal, December 2001


Highly efficient molecular delivery into mammalian cells using carbon nanotube spearing
journal, May 2005