Title: Interfacing nanostructures to biological cells

Abstract

Disclosed herein are methods and materials by which nanostructures such as carbon nanotubes, nanorods, etc. are bound to lectins and/or polysaccharides and prepared for administration to cells. Also disclosed are complexes comprising glycosylated nanostructures, which bind selectively to cells expressing glycosylated surface molecules recognized by the lectin. Exemplified is a complex comprising a carbon nanotube functionalized with a lipid-like alkane, linked to a polymer bearing repeated .alpha.-N-acetylgalactosamine sugar groups. This complex is shown to selectively adhere to the surface of living cells, without toxicity. In the exemplified embodiment, adherence is mediated by a multivalent lectin, which binds both to the cells and the .alpha.-N-acetylgalactosamine groups on the nanostructure.

Inventors:

Chen, Xing; Bertozzi, Carolyn R.; Zettl, Alexander K.

Issue Date:

Tue Sep 04 00:00:00 EDT 2012

Research Org.:

The Regents of the University of California (Oakland, CA); Lawrence Berkeley National Laboratory (LBNL), Berkeley, CA (United States)

Sponsoring Org.:

USDOE

OSTI Identifier:

1078298

Patent Number(s):

8257932

Application Number:

12/034,388

Assignee:

The Regents of the University of California (Oakland, CA)

Patent Classifications (CPCs):

A - HUMAN NECESSITIES A61 - MEDICAL OR VETERINARY SCIENCE A61K - PREPARATIONS FOR MEDICAL, DENTAL, OR TOILET PURPOSES

A - HUMAN NECESSITIES A61 - MEDICAL OR VETERINARY SCIENCE A61P - SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS

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A - HUMAN NECESSITIES A61 - MEDICAL OR VETERINARY SCIENCE A61K - PREPARATIONS FOR MEDICAL, DENTAL, OR TOILET PURPOSES

A61K47/549 - {Sugars, nucleosides, nucleotides or nucleic acids}
A61K47/58 - obtained by reactions only involving carbon-to-carbon unsaturated bonds, e.g. poly[meth]acrylate, polyacrylamide, polystyrene, polyvinylpyrrolidone, polyvinylalcohol or polystyrene sulfonic acid resin
A61K47/61 - the organic macromolecular compound being a polysaccharide or a derivative thereof
A61K47/6415 - {Toxins or lectins, e.g. clostridial toxins or Pseudomonas exotoxins}
A61K47/6949 - {inclusion complexes, e.g. clathrates, cavitates or fullerenes}

A - HUMAN NECESSITIES A61 - MEDICAL OR VETERINARY SCIENCE A61P - SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS

A61P43/00 - Drugs for specific purposes, not provided for in groups A61P1/00-A61P41/00

B - PERFORMING OPERATIONS B82 - NANOTECHNOLOGY B82Y - SPECIFIC USES OR APPLICATIONS OF NANOSTRUCTURES

B82Y5/00 - Nanobiotechnology or nanomedicine, e.g. protein engineering or drug delivery

C - CHEMISTRY C12 - BIOCHEMISTRY C12N - MICROORGANISMS OR ENZYMES

C12N2501/59 - Lectins
C12N5/0006 - {Modification of the membrane of cells, e.g. cell decoration}

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DOE Contract Number:  

AC02-05CH11231

Resource Type:

Patent

Country of Publication:

United States

Language:

English

Subject:

77 NANOSCIENCE AND NANOTECHNOLOGY; 59 BASIC BIOLOGICAL SCIENCES