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Title: Automated imaging system for single molecules

Abstract

There is provided a high throughput automated single molecule image collection and processing system that requires minimal initial user input. The unique features embodied in the present disclosure allow automated collection and initial processing of optical images of single molecules and their assemblies. Correct focus may be automatically maintained while images are collected. Uneven illumination in fluorescence microscopy is accounted for, and an overall robust imaging operation is provided yielding individual images prepared for further processing in external systems. Embodiments described herein are useful in studies of any macromolecules such as DNA, RNA, peptides and proteins. The automated image collection and processing system and method of same may be implemented and deployed over a computer network, and may be ergonomically optimized to facilitate user interaction.

Inventors:
; ;
Issue Date:
Research Org.:
Univ. of Wisconsin, Madison, WI (United States)
Sponsoring Org.:
USDOE
OSTI Identifier:
1078139
Patent Number(s):
8271251
Application Number:
60542469
Assignee:
Wisconsin Alumni Research Foundation
Patent Classifications (CPCs):
G - PHYSICS G06 - COMPUTING G06K - RECOGNITION OF DATA
DOE Contract Number:  
FG02-99ER62830
Resource Type:
Patent
Country of Publication:
United States
Language:
English
Subject:
47 OTHER INSTRUMENTATION

Citation Formats

Schwartz, David Charles, Runnheim, Rodney, and Forrest, Daniel. Automated imaging system for single molecules. United States: N. p., 2012. Web.
Schwartz, David Charles, Runnheim, Rodney, & Forrest, Daniel. Automated imaging system for single molecules. United States.
Schwartz, David Charles, Runnheim, Rodney, and Forrest, Daniel. Tue . "Automated imaging system for single molecules". United States. https://www.osti.gov/servlets/purl/1078139.
@article{osti_1078139,
title = {Automated imaging system for single molecules},
author = {Schwartz, David Charles and Runnheim, Rodney and Forrest, Daniel},
abstractNote = {There is provided a high throughput automated single molecule image collection and processing system that requires minimal initial user input. The unique features embodied in the present disclosure allow automated collection and initial processing of optical images of single molecules and their assemblies. Correct focus may be automatically maintained while images are collected. Uneven illumination in fluorescence microscopy is accounted for, and an overall robust imaging operation is provided yielding individual images prepared for further processing in external systems. Embodiments described herein are useful in studies of any macromolecules such as DNA, RNA, peptides and proteins. The automated image collection and processing system and method of same may be implemented and deployed over a computer network, and may be ergonomically optimized to facilitate user interaction.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {2012},
month = {9}
}

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Works referenced in this record:

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