Title: Compositions and methods for improved protein production

Abstract

The present invention relates to the identification of novel nucleic acid sequences, designated herein as 7p, 8k, 7E, 9G, 8Q and 203, in a host cell which effect protein production. The present invention also provides host cells having a mutation or deletion of part or all of the gene encoding 7p, 8k, 7E, 9G, 8Q and 203, which are presented in FIG. 1, and are SEQ ID NOS.: 1-6, respectively. The present invention also provides host cells further comprising a nucleic acid encoding a desired heterologous protein such as an enzyme.

Inventors:

Bodie, Elizabeth A ^[1]; Kim, Steve ^[2]

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+ Show Inventor Affiliations

1. San Carlos, CA
2. San Francisco, CA

Issue Date:

Tue Jul 10 00:00:00 EDT 2012

Research Org.:

National Renewable Energy Laboratory (NREL), Golden, CO (United States); Albany Research Center (ARC), Albany, OR (United States)

Sponsoring Org.:

USDOE

OSTI Identifier:

1069103

Patent Number(s):

8216844

Application Number:

12/376,558

Assignee:

Danisco US Inc. (Palo Alto, CA)

Patent Classifications (CPCs):

C - CHEMISTRY C07 - ORGANIC CHEMISTRY C07K - PEPTIDES

C - CHEMISTRY C12 - BIOCHEMISTRY C12N - MICROORGANISMS OR ENZYMES

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C - CHEMISTRY C07 - ORGANIC CHEMISTRY C07K - PEPTIDES

C07K14/37 - from fungi

C - CHEMISTRY C12 - BIOCHEMISTRY C12N - MICROORGANISMS OR ENZYMES

C12N15/01 - Preparation of mutants without inserting foreign genetic material therein
C12N15/80 - for fungi
C12N9/2437 - {Cellulases

C - CHEMISTRY C12 - BIOCHEMISTRY C12P - FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE {

C12P21/00 - Preparation of peptides or proteins
C12P21/02 - having a known sequence of two or more amino acids, e.g. glutathione

Y - NEW / CROSS SECTIONAL TECHNOLOGIES Y02 - TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE Y02P - CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS

Y02P20/52 - using catalysts, e.g. selective catalysts

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DOE Contract Number:  

AC36-99GO10337

Resource Type:

Patent

Country of Publication:

United States

Language:

English

Subject:

59 BASIC BIOLOGICAL SCIENCES