Compositions and methods for the expression of selenoproteins in eukaryotic cells
Abstract
Recombinant nucleic acid constructs for the efficient expression of eukaryotic selenoproteins and related methods for production of recombinant selenoproteins are provided. The nucleic acid constructs comprise novel selenocysteine insertion sequence (SECIS) elements. Certain novel SECIS elements of the invention contain non-canonical quartet sequences. Other novel SECIS elements provided by the invention are chimeric SECIS elements comprising a canonical SECIS element that contains a non-canonical quartet sequence and chimeric SECIS elements comprising a non-canonical SECIS element that contains a canonical quartet sequence. The novel SECIS elements of the invention facilitate the insertion of selenocysteine residues into recombinant polypeptides.
- Inventors:
-
- Lincoln, NE
- Puschino, RU
- Issue Date:
- Research Org.:
- Brookhaven National Laboratory (BNL), Upton, NY (United States)
- Sponsoring Org.:
- USDOE
- OSTI Identifier:
- 1067327
- Patent Number(s):
- 8273549
- Application Number:
- US Patent Application 12/428,007
- Assignee:
- Board of Regents of the University of Nebraska (Lincoln, NE)
- Patent Classifications (CPCs):
-
C - CHEMISTRY C12 - BIOCHEMISTRY C12P - FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE {
- DOE Contract Number:
- FG07-02ID14380
- Resource Type:
- Patent
- Country of Publication:
- United States
- Language:
- English
- Subject:
- 59 BASIC BIOLOGICAL SCIENCES
Citation Formats
Gladyshev, Vadim, and Novoselov, Sergey. Compositions and methods for the expression of selenoproteins in eukaryotic cells. United States: N. p., 2012.
Web.
Gladyshev, Vadim, & Novoselov, Sergey. Compositions and methods for the expression of selenoproteins in eukaryotic cells. United States.
Gladyshev, Vadim, and Novoselov, Sergey. Tue .
"Compositions and methods for the expression of selenoproteins in eukaryotic cells". United States. https://www.osti.gov/servlets/purl/1067327.
@article{osti_1067327,
title = {Compositions and methods for the expression of selenoproteins in eukaryotic cells},
author = {Gladyshev, Vadim and Novoselov, Sergey},
abstractNote = {Recombinant nucleic acid constructs for the efficient expression of eukaryotic selenoproteins and related methods for production of recombinant selenoproteins are provided. The nucleic acid constructs comprise novel selenocysteine insertion sequence (SECIS) elements. Certain novel SECIS elements of the invention contain non-canonical quartet sequences. Other novel SECIS elements provided by the invention are chimeric SECIS elements comprising a canonical SECIS element that contains a non-canonical quartet sequence and chimeric SECIS elements comprising a non-canonical SECIS element that contains a canonical quartet sequence. The novel SECIS elements of the invention facilitate the insertion of selenocysteine residues into recombinant polypeptides.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {Tue Sep 25 00:00:00 EDT 2012},
month = {Tue Sep 25 00:00:00 EDT 2012}
}
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