skip to main content
DOE Patents title logo U.S. Department of Energy
Office of Scientific and Technical Information

Title: Multiplex detection of respiratory pathogens

Abstract

Described are kits and methods useful for detection of respiratory pathogens (influenza A (including subtyping capability for H1, H3, H5 and H7 subtypes) influenza B, parainfluenza (type 2), respiratory syncytial virus, and adenovirus) in a sample. Genomic sequence information from the respiratory pathogens was analyzed to identify signature sequences, e.g., polynucleotide sequences useful for confirming the presence or absence of a pathogen in a sample. Primer and probe sets were designed and optimized for use in a PCR based, multiplexed Luminex assay to successfully identify the presence or absence of pathogens in a sample.

Inventors:
 [1];  [2];  [3]
  1. Brentwood, CA
  2. Livermore, CA
  3. Albany, CA
Issue Date:
Research Org.:
Lawrence Livermore National Lab. (LLNL), Livermore, CA (United States)
Sponsoring Org.:
USDOE
OSTI Identifier:
1058899
Patent Number(s):
8232058
Application Number:
12/161,291
Assignee:
Lawrence Livermore National Security, LLC (Livermore, CA)
Patent Classifications (CPCs):
C - CHEMISTRY C12 - BIOCHEMISTRY C12Q - MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS
DOE Contract Number:  
W-7405-ENG-48
Resource Type:
Patent
Country of Publication:
United States
Language:
English
Subject:
60 APPLIED LIFE SCIENCES

Citation Formats

McBride, Mary, Slezak, Thomas, and Birch, James M. Multiplex detection of respiratory pathogens. United States: N. p., 2012. Web.
McBride, Mary, Slezak, Thomas, & Birch, James M. Multiplex detection of respiratory pathogens. United States.
McBride, Mary, Slezak, Thomas, and Birch, James M. Tue . "Multiplex detection of respiratory pathogens". United States. https://www.osti.gov/servlets/purl/1058899.
@article{osti_1058899,
title = {Multiplex detection of respiratory pathogens},
author = {McBride, Mary and Slezak, Thomas and Birch, James M},
abstractNote = {Described are kits and methods useful for detection of respiratory pathogens (influenza A (including subtyping capability for H1, H3, H5 and H7 subtypes) influenza B, parainfluenza (type 2), respiratory syncytial virus, and adenovirus) in a sample. Genomic sequence information from the respiratory pathogens was analyzed to identify signature sequences, e.g., polynucleotide sequences useful for confirming the presence or absence of a pathogen in a sample. Primer and probe sets were designed and optimized for use in a PCR based, multiplexed Luminex assay to successfully identify the presence or absence of pathogens in a sample.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {2012},
month = {7}
}

Patent:

Save / Share:

Works referenced in this record:

Simultaneous Detection of Influenza Viruses A and B Using Real-Time Quantitative PCR
journal, January 2001


Rapid Detection and Identification of Human Adenovirus Species by Adenoplex, a Multiplex PCR-Enzyme Hybridization Assay
journal, September 2004


Multiplexed Reverse Transcriptase PCR Assay for Identification of Viral Respiratory Pathogens at the Point of Care
journal, September 2007


Influenza A viruses in feral Canadian ducks: extensive reassortment in nature
journal, August 2004


A computer program for selection of oligonucleotide primers for polymerase chain reactions
journal, January 1990


Large-Scale Sequence Analysis of Avian Influenza Isolates
journal, March 2006


A multiplexed PCR-coupled liquid bead array for the simultaneous detection of four biothreat agents
journal, April 2005


Comparison of Real-Time PCR Assays with Fluorescent-Antibody Assays for Diagnosis of Respiratory Virus Infections in Children
journal, July 2006


Evolutionary characterization of the six internal genes of H5N1 human influenza A virus
journal, May 2000


Research Profiles: Liquid array single-handedly detects bounty of BW agents
journal, May 2003


Stable and Noncompetitive RNA Internal Control for Routine Clinical Diagnostic Reverse Transcription-PCR
journal, March 2004


Design Strategies and Performance of Custom DNA Sequencing Primers
journal, September 1999


Evolution of H5 subtype avian influenza A viruses in North America
journal, October 1997


Multiplexed Liquid Arrays for Simultaneous Detection of Simulants of Biological Warfare Agents
journal, April 2003


Antigenic and genetic diversity among swine influenza A H1N1 and H1N2 viruses in Europe
journal, April 2002


Clinical and Financial Benefits of Rapid Detection of Respiratory Viruses: an Outcomes Study
journal, January 2000


Molecular changes associated with the transmission of avian influenza a H5N1 and H9N2 viruses to humans
journal, November 2001


Transmission of Eurasian avian H2 influenza virus to shorebirds in North America
journal, December 1999


Origin of the Pandemic 1957 H2 Influenza A Virus and the Persistence of Its Possible Progenitors in the Avian Reservoir
journal, June 1993