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Title: Genetically encoded fluorescent coumarin amino acids

Abstract

The invention relates to orthogonal pairs of tRNAs and aminoacyl-tRNA synthetases that can incorporate the coumarin unnatural amino acid L-(7-hydroxycoumarin-4-yl)ethylglycine into proteins produced in eubacterial host cells such as E. coli. The invention provides, for example but not limited to, novel orthogonal synthetases, methods for identifying and making the novel synthetases, methods for producing proteins containing the unnatural amino acid L-(7-hydroxycoumarin-4-yl)ethylglycine and related translation systems.

Inventors:
 [1];  [1];  [2]
  1. San Diego, CA
  2. La Jolla, CA
Issue Date:
Research Org.:
The Scripps Research Inst., La Jolla, CA (United States)
Sponsoring Org.:
USDOE
OSTI Identifier:
1055725
Patent Number(s):
8192952
Application Number:
12/227,383
Assignee:
The Scripps Research Institute (La Jolla, CA)
Patent Classifications (CPCs):
C - CHEMISTRY C12 - BIOCHEMISTRY C12N - MICROORGANISMS OR ENZYMES
C - CHEMISTRY C12 - BIOCHEMISTRY C12P - FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE {
DOE Contract Number:  
FG02-03ER46051
Resource Type:
Patent
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES

Citation Formats

Wang, Jiangyun, Xie, Jianming, and Schultz, Peter G. Genetically encoded fluorescent coumarin amino acids. United States: N. p., 2012. Web.
Wang, Jiangyun, Xie, Jianming, & Schultz, Peter G. Genetically encoded fluorescent coumarin amino acids. United States.
Wang, Jiangyun, Xie, Jianming, and Schultz, Peter G. Tue . "Genetically encoded fluorescent coumarin amino acids". United States. https://www.osti.gov/servlets/purl/1055725.
@article{osti_1055725,
title = {Genetically encoded fluorescent coumarin amino acids},
author = {Wang, Jiangyun and Xie, Jianming and Schultz, Peter G},
abstractNote = {The invention relates to orthogonal pairs of tRNAs and aminoacyl-tRNA synthetases that can incorporate the coumarin unnatural amino acid L-(7-hydroxycoumarin-4-yl)ethylglycine into proteins produced in eubacterial host cells such as E. coli. The invention provides, for example but not limited to, novel orthogonal synthetases, methods for identifying and making the novel synthetases, methods for producing proteins containing the unnatural amino acid L-(7-hydroxycoumarin-4-yl)ethylglycine and related translation systems.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {2012},
month = {6}
}

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Works referenced in this record:

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