Assay for vitamin B12 absorption and method of making labeled vitamin B12
Abstract
The invention provides methods for labeling vitamin B12 with .sup.14C, .sup.13C, tritium, and deuterium. When radioisotopes are used, the invention provides for methods of labeling B12 with high specific activity. The invention also provides labeled vitamin B12 compositions made in accordance with the invention.
- Inventors:
-
- Davis, CA
- Elmacero, CA
- Silver Spring, MD
- Orinda, CA
- Issue Date:
- Research Org.:
- Lawrence Livermore National Laboratory (LLNL), Livermore, CA (United States)
- Sponsoring Org.:
- USDOE
- OSTI Identifier:
- 1055506
- Patent Number(s):
- 8202507
- Application Number:
- 11/234,079
- Assignee:
- The Regents of the University of California (Oakland, CA)
- Patent Classifications (CPCs):
-
A - HUMAN NECESSITIES A61 - MEDICAL OR VETERINARY SCIENCE A61K - PREPARATIONS FOR MEDICAL, DENTAL, OR TOILET PURPOSES
C - CHEMISTRY C12 - BIOCHEMISTRY C12P - FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE {
- DOE Contract Number:
- W-7405-ENG-48
- Resource Type:
- Patent
- Country of Publication:
- United States
- Language:
- English
- Subject:
- 60 APPLIED LIFE SCIENCES
Citation Formats
Anderson, Peter J, Dueker, Stephen, Miller, Joshua, Green, Ralph, Roth, John, Carkeet, Colleen, and Buchholz, Bruce A. Assay for vitamin B12 absorption and method of making labeled vitamin B12. United States: N. p., 2012.
Web.
Anderson, Peter J, Dueker, Stephen, Miller, Joshua, Green, Ralph, Roth, John, Carkeet, Colleen, & Buchholz, Bruce A. Assay for vitamin B12 absorption and method of making labeled vitamin B12. United States.
Anderson, Peter J, Dueker, Stephen, Miller, Joshua, Green, Ralph, Roth, John, Carkeet, Colleen, and Buchholz, Bruce A. Tue .
"Assay for vitamin B12 absorption and method of making labeled vitamin B12". United States. https://www.osti.gov/servlets/purl/1055506.
@article{osti_1055506,
title = {Assay for vitamin B12 absorption and method of making labeled vitamin B12},
author = {Anderson, Peter J and Dueker, Stephen and Miller, Joshua and Green, Ralph and Roth, John and Carkeet, Colleen and Buchholz, Bruce A.},
abstractNote = {The invention provides methods for labeling vitamin B12 with .sup.14C, .sup.13C, tritium, and deuterium. When radioisotopes are used, the invention provides for methods of labeling B12 with high specific activity. The invention also provides labeled vitamin B12 compositions made in accordance with the invention.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {Tue Jun 19 00:00:00 EDT 2012},
month = {Tue Jun 19 00:00:00 EDT 2012}
}
Works referenced in this record:
Prospective evaluation of protein bound vitamin B 12 (cobalamin) malabsorption in the elderly using trout flesh labelled in vivo with 57 Co-cobalamin
journal, October 1997
- Aimone-Gastin, I.; Pierson, H.; Jeandel, C.
- Gut, Vol. 41, Issue 4
Biosynthesis of the 5,6-dimethylbenzimidazole moiety of vitamin B12
journal, September 1969
- Alworth, William L.; Baker, Harold Nordean; Lee, Dwight Augustus
- Journal of the American Chemical Society, Vol. 91, Issue 20
Biosynthesis of vitamin B12 in anaerobic bacteria. Transformation of 5-hydroxybenzimidazole and 5-hydroxy-6-methylbenzimidazole into 5,6-dimethylbenzimidazole in Eubacterium limosum
journal, November 1993
- Renz, Paul; Endres, Birgit; Kurz, Birgit
- European Journal of Biochemistry, Vol. 217, Issue 3
Cobalamin and Folate Evaluation: Measurement of Methylmalonic Acid and Homocysteine vs Vitamin B12 and Folate
journal, August 2000
- Klee, George G.
- Clinical Chemistry, Vol. 46, Issue 8
Evaluation of Biosynthetic Pathways to δ-Aminolevulinic Acid in Propionibacterium shermanii Based on Biosynthesis of Vitamin B 12 from d -[1- 13 C]Glucose
journal, April 2000
- Iida, Katsumi; Kajiwara, Masahiro
- Biochemistry, Vol. 39, Issue 13
Biosynthesis of vitamin B12 in anaerobic bacteria . Experiments with Eubacterium limosum on the transformation of 5-hydroxy-6-methyl-benzimidazole, its nucleoside, its cobamide, and of 5-hydroxybenzimidazolylcobamide in vitamin B12
journal, June 1998
- Schulze, Bettina; Vogler, Bernhard; Renz, Paul
- European Journal of Biochemistry, Vol. 254, Issue 3
Biosynthesis of cobalamin in Salmonella typhimurium : transformation of riboflavin into the 5,6-dimethylbenzimidazole moiety
journal, December 1998
- Keck, Bettina; Munder, Michael; Renz, P.
- Archives of Microbiology, Vol. 171, Issue 1
Purification and Characterization of CobT, the Nicotinate-mononucleotide:5,6-Dimethylbenzimidazole Phosphoribosyltransferase Enzyme from Salmonella typhimurium LT2
journal, July 1997
- Trzebiatowski, Jodi R.; Escalante-Semerena, Jorge C.
- Journal of Biological Chemistry, Vol. 272, Issue 28
Biosynthesis of vitamin B12 in anaerobic bacteria. Experiments with Eubacterium limosum on the incorporation of D-[1-13C]erythrose and [13C]formate into the 5,6-dimethylbenzimidazole moiety
journal, March 1992
- Munder, Michael; Vogt, Joachim R. A.; Vogler, Bernhard
- European Journal of Biochemistry, Vol. 204, Issue 2