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Title: Methods and composition for the production of orthogonal tRNA-aminoacyltRNA synthetase pairs

Abstract

This invention provides compositions and methods for generating components of protein biosynthetic machinery including orthogonal tRNAs, orthogonal aminoacyl-tRNA synthetases, and orthogonal pairs of tRNAs/synthetases. Methods for identifying orthogonal pairs are also provided. These components can be used to incorporate unnatural amino acids into proteins in vivo.

Inventors:
 [1];  [2];  [2];  [3];  [4];  [5];  [6];  [7];  [2];  [8];  [2]
  1. La Jolla, CA
  2. San Diego, CA
  3. Cambridge, GB
  4. Lexington, MA
  5. North Haven, CT
  6. Philadelphia, PA
  7. Lancaster, PA
  8. Cambridge, MA
Issue Date:
Research Org.:
The Scripps Research Institute (LaJolla, CA)
Sponsoring Org.:
USDOE
OSTI Identifier:
1042628
Patent Number(s):
8183012
Application Number:
11/009,635
Assignee:
The Scripps Research Institute (LaJolla, CA);The Regents of the University of California (Oakland, CA)
Patent Classifications (CPCs):
C - CHEMISTRY C12 - BIOCHEMISTRY C12P - FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE {
C - CHEMISTRY C12 - BIOCHEMISTRY C12N - MICROORGANISMS OR ENZYMES
DOE Contract Number:  
FG03-00ER45812; AC03-76SF00098
Resource Type:
Patent
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES

Citation Formats

Schultz, Peter G, Wang, Lei, Anderson, John Christopher, Chin, Jason, Liu, David R, Magliery, Thomas J, Meggers, Eric L, Mehl, Ryan Aaron, Pastrnak, Miro, Santoro, Steven William, and Zhang, Zhiwen. Methods and composition for the production of orthogonal tRNA-aminoacyltRNA synthetase pairs. United States: N. p., 2012. Web.
Schultz, Peter G, Wang, Lei, Anderson, John Christopher, Chin, Jason, Liu, David R, Magliery, Thomas J, Meggers, Eric L, Mehl, Ryan Aaron, Pastrnak, Miro, Santoro, Steven William, & Zhang, Zhiwen. Methods and composition for the production of orthogonal tRNA-aminoacyltRNA synthetase pairs. United States.
Schultz, Peter G, Wang, Lei, Anderson, John Christopher, Chin, Jason, Liu, David R, Magliery, Thomas J, Meggers, Eric L, Mehl, Ryan Aaron, Pastrnak, Miro, Santoro, Steven William, and Zhang, Zhiwen. Tue . "Methods and composition for the production of orthogonal tRNA-aminoacyltRNA synthetase pairs". United States. https://www.osti.gov/servlets/purl/1042628.
@article{osti_1042628,
title = {Methods and composition for the production of orthogonal tRNA-aminoacyltRNA synthetase pairs},
author = {Schultz, Peter G and Wang, Lei and Anderson, John Christopher and Chin, Jason and Liu, David R and Magliery, Thomas J and Meggers, Eric L and Mehl, Ryan Aaron and Pastrnak, Miro and Santoro, Steven William and Zhang, Zhiwen},
abstractNote = {This invention provides compositions and methods for generating components of protein biosynthetic machinery including orthogonal tRNAs, orthogonal aminoacyl-tRNA synthetases, and orthogonal pairs of tRNAs/synthetases. Methods for identifying orthogonal pairs are also provided. These components can be used to incorporate unnatural amino acids into proteins in vivo.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {2012},
month = {5}
}

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