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Title: Method for voltage-gated protein fractionation

Abstract

We report unique findings on the voltage dependence of protein exclusion from the pores of nanoporous polymer exclusion membranes. The pores are small enough that proteins are excluded from passage with low applied electric fields, but increasing the field enables proteins to pass through. The requisite field necessary for a change in exclusion is protein-specific with a correlation to protein size. The field-dependence of exclusion is important to consider for preconcentration applications. The ability to selectively gate proteins at exclusion membranes is also a promising means for manipulating and characterizing proteins. We show that field-gated exclusion can be used to selectively remove proteins from a mixture, or to selectively trap protein at one exclusion membrane in a series.

Inventors:
 [1];  [2]
  1. Tracy, CA
  2. Danville, CA
Issue Date:
Research Org.:
Sandia National Laboratories (SNL), Albuquerque, NM, and Livermore, CA (United States)
Sponsoring Org.:
USDOE
OSTI Identifier:
1040702
Patent Number(s):
8163154
Application Number:
11/779,407
Assignee:
Sandia Corporation (Albuquerque, NM)
Patent Classifications (CPCs):
G - PHYSICS G01 - MEASURING G01N - INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
B - PERFORMING OPERATIONS B01 - PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL B01D - SEPARATION
DOE Contract Number:  
AC04-94AL85000
Resource Type:
Patent
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES

Citation Formats

Hatch, Anson, and Singh, Anup K. Method for voltage-gated protein fractionation. United States: N. p., 2012. Web.
Hatch, Anson, & Singh, Anup K. Method for voltage-gated protein fractionation. United States.
Hatch, Anson, and Singh, Anup K. Tue . "Method for voltage-gated protein fractionation". United States. https://www.osti.gov/servlets/purl/1040702.
@article{osti_1040702,
title = {Method for voltage-gated protein fractionation},
author = {Hatch, Anson and Singh, Anup K},
abstractNote = {We report unique findings on the voltage dependence of protein exclusion from the pores of nanoporous polymer exclusion membranes. The pores are small enough that proteins are excluded from passage with low applied electric fields, but increasing the field enables proteins to pass through. The requisite field necessary for a change in exclusion is protein-specific with a correlation to protein size. The field-dependence of exclusion is important to consider for preconcentration applications. The ability to selectively gate proteins at exclusion membranes is also a promising means for manipulating and characterizing proteins. We show that field-gated exclusion can be used to selectively remove proteins from a mixture, or to selectively trap protein at one exclusion membrane in a series.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {2012},
month = {4}
}

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Works referenced in this record:

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